RchiOBHm_Chr5g0020541

APO protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0026692
Physical Location & Seq
Reverse (-)
14774003 .. 14776958
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30062

Sequence Viewer

Length: 1338 bp
ATGTTCCAGAGGCCTCCAACAGCATCCTCTACTTTGTGGGAACCGTCTCCGAAAGGTGCTTGCCTCAGTACCGTAGATTTCAAGCCGCCTCAATTATCAGCTGCGAGTTCATATAATCTTGGCTTGAAGTTTGAGCATGGAAAGCTTCGTAAAGGGGGAAGTACATGCAGAACCATCTACTCTGTCAGTAGGAAGCCCAGTGTAGAACCAACTTTCAGGAAGCGAGATGCATCTCCCCAAAATGAGGATCTTCCAGCTGTACTACCTAAGCAAAAGAAAAAACCATATCCCATTCCCTTAGAGAAGATTAAGCAGGCTGCAAAGGTGAACAAAAAACTTGCAGAAAAGGGTATAGAAAAGCCACTCGACCCTCCAAAAAATGGATTACTTGACCCTGATCTAATTCCTGTTGCTCATCAAGTACTGCATGCTTGGAAAGCTTTGATTAAAGGCCTTGCACAGCTGTTGCATGTTATTCCAGTATATGGTTGCCTTGAATGCTCTGAGGTCCATGTAGCCCAGTCTGGCCATCACATTCAGGACTGTCTTGGTCCATCAAATGCTACACGCCGAAGCTTCCACTCATGGATGAAGGGTTCAATCAATGACGTACTTGTACCCATTGAAGCATATCATCTCTACGATCCTTTTGGTCGGCGCATTAAGCATGAAACTAGATTCCAATATGACAGGATTCCAGCAGTTGTGGAGCTGTGCATCCAAGCTGGTGTGGACATACCTGAGTACCCTTCACGCCGAAGAACCAAACCCATCCGAATGATCGGAAAGAAAGTGATCGATCGGGGTGGATTTGTTGAGGAGCCTGAGCAATGGCATGCTGCAAATCCATCTTCACTTGCTATTGCTGATCTTGATACACATGGGGCTTCTGAATGGTTTGCACCTCCACTGTCATCAGATATTCCTAAAATTGCACAGGAGACACTAGATGCATATGAAACTGTCAAGTCTGGAGTGACAAAGCTGATGAAGAAATGCAGTGTGAAGGCATGTGGGTATTGCACGGAGGTTCATGTAGGACCTTGGGGTCATAATGCAAAGCTGTGTGGAGAATTCAAGCACCAATGGAGGGATGGAAAACATGGTTGGCAGGATGCTACCGTGGACGAAGTTTTCCCTCCAAATTATGTATGGCATGTCAAAGATCCCAAAGGGCCTCCAATGAAGGGTGGTGCGCTCAAGAAGTTCTACGGGAAGGTGCCAGCAGTGGTAGAGCTGTGCTTGCAAGCAGGTGCTCAGATTCCTGAGAAATACAAGCCCATGATGAGGCTTGACATTGTAGTCCCTGAAATTGATGAGGCACAGTTAGTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

445

Amino Acids

49.56

Weight (kDa)

9.13

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 71 - 267 6.4e-102 APO RNA-binding
APO_RNA-bind PF05634 309 - 423 6.8e-24 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026692)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0020541
rosa_samantha Rh5CG161800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1241
AasI GACNNNNNNGTC 2 cut(s) 1047, 1301
Acc36I ACCTGC 1 cut(s) 1241
AccB1I GGYRCC 1 cut(s) 1219
AccB7I CCANNNNNTGG 2 cut(s) 380, 485
AciI CCGC 1 cut(s) 86
AclWI GGATC 3 cut(s) 255, 638, 1160
AcoI YGGCCR 1 cut(s) 526
AcsI RAATTY 1 cut(s) 1073
AfaI GTAC 7 cut(s) 70, 163, 261, 423, 612, 618, 746
AfiI CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1090, 1187, 1287
AgsI TTSAA 6 cut(s) 82, 127, 497, 600, 626, 1078
AjuI GAANNNNNNNTTGG 2 cut(s) 1090, 1122
AloI GAACNNNNNNTCC 2 cut(s) 580, 612
Alw21I GWGCWC 1 cut(s) 1258
Alw26I GTCTC 2 cut(s) 51, 935
AlwI GGATC 3 cut(s) 255, 638, 1160
AoxI GGCC 4 cut(s) 11, 451, 526, 1175
ApeKI GCWGC 3 cut(s) 101, 317, 839
ApoI RAATTY 1 cut(s) 1073
AspLEI GCGC 2 cut(s) 660, 1198
AspS9I GGNCC 4 cut(s) 508, 551, 1040, 1175
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 3 cut(s) 508, 551, 1040
BaeI ACNNNNGTAYC 2 cut(s) 728, 761
BalI TGGCCA 1 cut(s) 528
BanI GGYRCC 1 cut(s) 1219
Bbv12I GWGCWC 1 cut(s) 1258
BbvI GCAGC 3 cut(s) 88, 304, 826
BccI CCATC 6 cut(s) 182, 537, 562, 779, 856, 1088
BcoDI GTCTC 2 cut(s) 51, 935
BfaI CTAG 2 cut(s) 675, 947
BfuAI ACCTGC 1 cut(s) 1241
BisI GCNGC 4 cut(s) 86, 102, 318, 840
BlsI GCNGC 4 cut(s) 87, 103, 319, 841
BmcAI AGTACT 1 cut(s) 423
Bme18I GGWCC 3 cut(s) 508, 551, 1040
BmgT120I GGNCC 4 cut(s) 508, 551, 1040, 1175
BmiI GGNNCC 3 cut(s) 42, 822, 1221
BmrI ACTGGG 2 cut(s) 192, 514
BmsI GCATC 6 cut(s) 32, 217, 239, 726, 940, 1105
BmuI ACTGGG 2 cut(s) 192, 514
BpmI CTGGAG 1 cut(s) 993
Bpu10I CCTNAGC 2 cut(s) 267, 825
BpuEI CTTGAG 1 cut(s) 1184
Bsa29I ATCGAT 1 cut(s) 798
BsaJI CCNNGG 2 cut(s) 1043, 1122
Bsc4I CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1090, 1187, 1287
Bse1I ACTGG 3 cut(s) 198, 479, 520
Bse3DI GCAATG 1 cut(s) 836
BseCI ATCGAT 1 cut(s) 798
BseDI CCNNGG 2 cut(s) 1043, 1122
BseGI GGATG 6 cut(s) 23, 594, 717, 771, 1099, 1120
BseLI CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1090, 1187, 1287
BseMI GCAATG 1 cut(s) 836
BseMII CTCAG 6 cut(s) 79, 495, 732, 816, 1257, 1271
BseNI ACTGG 3 cut(s) 198, 479, 520
BseRI GAGGAG 1 cut(s) 833
BseXI GCAGC 3 cut(s) 88, 304, 826
Bsh1285I CGRYCG 1 cut(s) 802
BshFI GGCC 4 cut(s) 13, 453, 528, 1177
BshNI GGYRCC 1 cut(s) 1219
BshVI ATCGAT 1 cut(s) 798
BsiEI CGRYCG 1 cut(s) 802
BsiHKAI GWGCWC 1 cut(s) 1258
BslFI GGGAC 1 cut(s) 1289
BslI CCNNNNNNNGG 6 cut(s) 244, 380, 485, 1090, 1187, 1287
BsmAI GTCTC 2 cut(s) 51, 935
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 1289
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 4 cut(s) 13, 453, 528, 1177
Bsp1286I GDGCHC 1 cut(s) 1258
Bsp143I GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
BspACI CCGC 1 cut(s) 86
BspANI GGCC 4 cut(s) 13, 453, 528, 1177
BspCNI CTCAG 6 cut(s) 78, 496, 733, 817, 1258, 1270
BspDI ATCGAT 1 cut(s) 798
BspLI GGNNCC 3 cut(s) 42, 822, 1221
BspMI ACCTGC 1 cut(s) 1241
BspPI GGATC 3 cut(s) 255, 638, 1160
BspT107I GGYRCC 1 cut(s) 1219
BsrDI GCAATG 1 cut(s) 836
BsrI ACTGG 3 cut(s) 198, 479, 520
BssECI CCNNGG 2 cut(s) 1043, 1122
BssMI GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
BssT1I CCWWGG 1 cut(s) 1043
Bst4CI ACNGT 7 cut(s) 45, 73, 545, 912, 964, 1123, 1326
BstC8I GCNNGC 7 cut(s) 61, 315, 429, 837, 1224, 1244, 1248
BstDEI CTNAG 8 cut(s) 65, 267, 298, 504, 741, 825, 1257, 1266
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 6 cut(s) 23, 594, 717, 771, 1099, 1120
BstHHI GCGC 2 cut(s) 660, 1198
BstKTI GATC 8 cut(s) 250, 400, 646, 783, 798, 802, 871, 1168
BstMAI GTCTC 2 cut(s) 51, 935
BstMBI GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
BstMCI CGRYCG 1 cut(s) 802
BstMWI GCNNNNNNNGC 5 cut(s) 142, 437, 498, 664, 1243
BstNSI RCATGY 6 cut(s) 168, 431, 473, 839, 1014, 1160
BstV1I GCAGC 3 cut(s) 88, 304, 826
BstX2I RGATCY 2 cut(s) 247, 1165
BstYI RGATCY 2 cut(s) 247, 1165
Bsu15I ATCGAT 1 cut(s) 798
BsuRI GGCC 4 cut(s) 13, 453, 528, 1177
BsuTUI ATCGAT 1 cut(s) 798
BtgI CCRYGG 1 cut(s) 1122
BtsCI GGATG 6 cut(s) 23, 594, 717, 771, 1099, 1120
BtsI GCAGTG 2 cut(s) 1006, 1233
BtsIMutI CAGTG 4 cut(s) 205, 908, 1006, 1233
BveI ACCTGC 1 cut(s) 1241
Cac8I GCNNGC 7 cut(s) 61, 315, 429, 837, 1224, 1244, 1248
CfoI GCGC 2 cut(s) 660, 1198
Cfr13I GGNCC 4 cut(s) 508, 551, 1040, 1175
ClaI ATCGAT 1 cut(s) 798
Csp6I GTAC 7 cut(s) 69, 162, 260, 422, 611, 617, 745
CviQI GTAC 7 cut(s) 69, 162, 260, 422, 611, 617, 745
DdeI CTNAG 8 cut(s) 65, 267, 298, 504, 741, 825, 1257, 1266
DpnI GATC 8 cut(s) 249, 399, 645, 782, 797, 801, 870, 1167
DpnII GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
DrdI GACNNNNNNGTC 2 cut(s) 1047, 1301
DseDI GACNNNNNNGTC 2 cut(s) 1047, 1301
EaeI YGGCCR 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 1043
Eco147I AGGCCT 2 cut(s) 13, 453
Eco47I GGWCC 3 cut(s) 508, 551, 1040
EcoO109I RGGNCCY 2 cut(s) 1040, 1175
EcoRI GAATTC 1 cut(s) 1073
EcoT14I CCWWGG 1 cut(s) 1043
EcoT22I ATGCAT 2 cut(s) 232, 955
ErhI CCWWGG 1 cut(s) 1043
Esp3I CGTCTC 1 cut(s) 51
FaqI GGGAC 1 cut(s) 1289
FauNDI CATATG 1 cut(s) 955
Fnu4HI GCNGC 4 cut(s) 86, 102, 318, 840
FokI GGATG 6 cut(s) 10, 601, 704, 758, 1106, 1127
Fsp4HI GCNGC 4 cut(s) 86, 102, 318, 840
FspBI CTAG 2 cut(s) 675, 947
GlaI GCGC 2 cut(s) 659, 1197
GluI GCNGC 4 cut(s) 86, 102, 318, 840
GsuI CTGGAG 1 cut(s) 993
HaeIII GGCC 4 cut(s) 13, 453, 528, 1177
HhaI GCGC 2 cut(s) 660, 1198
Hin6I GCGC 2 cut(s) 658, 1196
HinP1I GCGC 2 cut(s) 658, 1196
HindIII AAGCTT 3 cut(s) 143, 438, 574
HinfI GANTC 3 cut(s) 678, 694, 1261
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 3 cut(s) 328, 733, 1126
Hpy188I TCNGA 7 cut(s) 51, 505, 776, 785, 892, 919, 1260
Hpy188III TCNNGA 7 cut(s) 7, 217, 539, 872, 972, 1201, 1265
Hpy8I GTNNAC 3 cut(s) 328, 733, 1126
HpyAV CCTTC 5 cut(s) 586, 759, 1000, 1180, 1210
HpyCH4III ACNGT 7 cut(s) 45, 73, 545, 912, 964, 1123, 1326
HpyCH4IV ACGT 1 cut(s) 609
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 437, 498, 664, 1243
HpyF3I CTNAG 8 cut(s) 65, 267, 298, 504, 741, 825, 1257, 1266
HpySE526I ACGT 1 cut(s) 609
HspAI GCGC 2 cut(s) 658, 1196
Kzo9I GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
LmnI GCTCC 2 cut(s) 709, 820
Lsp1109I GCAGC 3 cut(s) 88, 304, 826
LweI GCATC 6 cut(s) 32, 217, 239, 726, 940, 1105
MaeI CTAG 2 cut(s) 675, 947
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 1 cut(s) 976
MalI GATC 8 cut(s) 249, 399, 645, 782, 797, 801, 870, 1167
MboI GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
MboII GAAGA 5 cut(s) 242, 316, 771, 843, 1003
MflI RGATCY 2 cut(s) 247, 1165
MhlI GDGCHC 1 cut(s) 1258
MlsI TGGCCA 1 cut(s) 528
MluCI AATT 6 cut(s) 92, 402, 930, 1073, 1144, 1311
MluNI TGGCCA 1 cut(s) 528
MmeI TCCRAC 1 cut(s) 41
Mox20I TGGCCA 1 cut(s) 528
Mph1103I ATGCAT 2 cut(s) 232, 955
MscI TGGCCA 1 cut(s) 528
MseI TTAA 3 cut(s) 309, 447, 663
MslI CAYNNNNRTG 1 cut(s) 776
Msp20I TGGCCA 1 cut(s) 528
MspA1I CMGCKG 3 cut(s) 101, 257, 463
Mva1269I GAATGC 1 cut(s) 503
MwoI GCNNNNNNNGC 5 cut(s) 142, 437, 498, 664, 1243
NdeI CATATG 1 cut(s) 955
NdeII GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
NlaIV GGNNCC 3 cut(s) 42, 822, 1221
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 2 cut(s) 232, 955
NspI RCATGY 6 cut(s) 168, 431, 473, 839, 1014, 1160
PaeI GCATGC 2 cut(s) 431, 839
PaqCI CACCTGC 1 cut(s) 1241
PceI AGGCCT 2 cut(s) 13, 453
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 3 cut(s) 678, 694, 1261
PflMI CCANNNNNTGG 2 cut(s) 380, 485
PkrI GCNGC 4 cut(s) 87, 103, 319, 841
Ple19I CGATCG 1 cut(s) 802
PpuMI RGGWCCY 1 cut(s) 1040
Psp5II RGGWCCY 1 cut(s) 1040
PspN4I GGNNCC 3 cut(s) 42, 822, 1221
PspPI GGNCC 4 cut(s) 508, 551, 1040, 1175
PspPPI RGGWCCY 1 cut(s) 1040
PsuI RGATCY 2 cut(s) 247, 1165
PvuI CGATCG 1 cut(s) 802
PvuII CAGCTG 3 cut(s) 101, 257, 463
RsaI GTAC 7 cut(s) 70, 163, 261, 423, 612, 618, 746
RsaNI GTAC 7 cut(s) 69, 162, 260, 422, 611, 617, 745
RseI CAYNNNNRTG 1 cut(s) 776
SaqAI TTAA 3 cut(s) 309, 447, 663
SatI GCNGC 4 cut(s) 86, 102, 318, 840
Sau3AI GATC 8 cut(s) 247, 397, 643, 780, 795, 799, 868, 1165
Sau96I GGNCC 4 cut(s) 508, 551, 1040, 1175
ScaI AGTACT 1 cut(s) 423
SduI GDGCHC 1 cut(s) 1258
SfaNI GCATC 6 cut(s) 32, 217, 239, 726, 940, 1105
SinI GGWCC 3 cut(s) 508, 551, 1040
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 1199
SmoI CTYRAG 1 cut(s) 1199
SphI GCATGC 2 cut(s) 431, 839
Sse9I AATT 6 cut(s) 92, 402, 930, 1073, 1144, 1311
SseBI AGGCCT 2 cut(s) 13, 453
SsiI CCGC 1 cut(s) 86
SspMI CTAG 2 cut(s) 675, 947
StuI AGGCCT 2 cut(s) 13, 453
StyI CCWWGG 1 cut(s) 1043
TaaI ACNGT 7 cut(s) 45, 73, 545, 912, 964, 1123, 1326
TaiI ACGT 1 cut(s) 612
TaqI TCGA 2 cut(s) 366, 798
TasI AATT 6 cut(s) 92, 402, 930, 1073, 1144, 1311
TatI WGTACW 3 cut(s) 161, 259, 421
TauI GCSGC 1 cut(s) 88
TfiI GAWTC 3 cut(s) 678, 694, 1261
Tru1I TTAA 3 cut(s) 309, 447, 663
Tru9I TTAA 3 cut(s) 309, 447, 663
TscAI CASTG 4 cut(s) 205, 915, 1006, 1233
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 3 cut(s) 101, 317, 839
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 7 cut(s) 99, 605, 684, 972, 1004, 1022, 1199
TspGWI ACGGA 1 cut(s) 1040
TspRI CASTG 4 cut(s) 205, 915, 1006, 1233
Van91I CCANNNNNTGG 2 cut(s) 380, 485
VpaK11BI GGWCC 3 cut(s) 508, 551, 1040
XapI RAATTY 1 cut(s) 1073
XceI RCATGY 6 cut(s) 168, 431, 473, 839, 1014, 1160
XcmI CCANNNNNNNNNTGG 2 cut(s) 1091, 1149
XspI CTAG 2 cut(s) 675, 947
ZrmI AGTACT 1 cut(s) 423
Zsp2I ATGCAT 2 cut(s) 232, 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.