RchiOBHm_Chr7g0205221

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0030470
Physical Location & Seq
Forward (+)
22872659 .. 22873375
717 bp
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UTR
Exon/CDS
Intron
PRQ18364

Sequence Viewer

Length: 717 bp
ATGAAGGTCGACGAGAGACGGTTTTTTAGCAGCGGCGTATATCAAACATTTCCTGAAACAGAACCCCTTCCCTCCGAGCATGAGGCGGACCTGGTTCCGATCAGATTTACGCTGTGGAATTACTTGGAAAGGTTTATTATAAAACATGACGGTCAGCCATTACAAGGTCGACCTCACCGGGAAGAATCAACCCTCTTGAAAGAGGAAGTGATTAGGGTTTCTCTGCCCAGCCTCAAGCTTTCATCTAGGAGACGTGAGCACAGAGAGACCTTGATTGAGATTGTTTCCCGAATTTACGATCATATCCTAGAAGATAGGCTTCGAGCTATTGTTGATGAGATTTTTGAGGTGGTTGATGATGAACGGACCGTCCCCTTGCGGCCTATTAGTGTGAGAGGATCGAACGTGAATTTGAGTCTCTACTGTTCTAGGCCTCTAACGGTTCCAGTCGATACCATTCACCTGCCATTTATTCCTCCTCCTGCATTTAAAACATCACCGGCTTTCGAATCATTGAATTTGGAGAAAGTGGAACTTGATAGTTTGGAAGCTGCAGAGAAATCATGTGTTATTTGCATCGAGGACTTGGAGGCCGGTGTCCAAGTTATCCGCTTGCCTTGTTCGCACCTTTACCATGAACGATGCATCGATCGGTGGCTCAAGAAGCCTAGTCATATATGTCCCATTTGTCGATCTCCGATCTGCCGCAGACTTTAG

Protein Analysis

238

Amino Acids

27.68

Weight (kDa)

6.16

Isoelectric Point (pI)

67.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 184 - 231 6.5e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 188 - 231 9.6e-14 Ring finger domain
zf-RING_11 PF17123 189 - 216 3.2e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 189 - 230 5.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 189 - 231 5.3e-06 zinc-RING finger domain
zf-C3HC4 PF00097 189 - 230 5.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0030470)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0205221
rosa_multiflora Rmu_sc0009349.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 140
AarI CACCTGC 1 cut(s) 471
Acc36I ACCTGC 1 cut(s) 471
AccI GTMKAC 2 cut(s) 9, 169
AciI CCGC 5 cut(s) 33, 86, 379, 610, 706
AclWI GGATC 1 cut(s) 406
AcsI RAATTY 3 cut(s) 291, 409, 517
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 2 cut(s) 199, 517
AjiI CACGTC 1 cut(s) 254
AjnI CCWGG 1 cut(s) 90
AloI GAACNNNNNNTCC 2 cut(s) 354, 386
AluBI AGCT 3 cut(s) 238, 326, 551
AluI AGCT 3 cut(s) 238, 326, 551
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 4 cut(s) 10, 244, 260, 422
AlwI GGATC 1 cut(s) 406
AoxI GGCC 3 cut(s) 380, 431, 591
ApeKI GCWGC 2 cut(s) 30, 551
ApoI RAATTY 3 cut(s) 291, 409, 517
Asp700I GAANNNNTTC 1 cut(s) 66
AspS9I GGNCC 2 cut(s) 88, 366
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 167, 452, 489
AsuII TTCGAA 1 cut(s) 507
AvaII GGWCC 2 cut(s) 88, 366
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 42, 538
BciT130I CCWGG 1 cut(s) 92
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 4 cut(s) 10, 244, 260, 422
BfaI CTAG 4 cut(s) 246, 308, 429, 669
BfmI CTRYAG 1 cut(s) 552
BfuAI ACCTGC 1 cut(s) 471
BisI GCNGC 5 cut(s) 31, 34, 380, 552, 706
BlsI GCNGC 5 cut(s) 32, 35, 381, 553, 707
Bme1390I CCNGG 2 cut(s) 92, 179
Bme18I GGWCC 2 cut(s) 88, 366
BmgBI CACGTC 1 cut(s) 254
BmgT120I GGNCC 2 cut(s) 88, 366
BmiI GGNNCC 2 cut(s) 96, 444
BmrFI CCNGG 2 cut(s) 92, 179
BmsI GCATC 3 cut(s) 585, 632, 654
Bpu14I TTCGAA 1 cut(s) 507
BpuEI CTTGAG 2 cut(s) 218, 644
BpuMI CCSGG 1 cut(s) 179
Bsa29I ATCGAT 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse118I RCCGGY 2 cut(s) 499, 593
Bse1I ACTGG 1 cut(s) 446
BseBI CCWGG 1 cut(s) 92
BseCI ATCGAT 1 cut(s) 648
BseLI CCNNNNNNNGG 1 cut(s) 164
BseNI ACTGG 1 cut(s) 446
BseRI GAGGAG 1 cut(s) 468
BseXI GCAGC 2 cut(s) 42, 538
BseYI CCCAGC 1 cut(s) 227
Bsh1285I CGRYCG 1 cut(s) 652
BshFI GGCC 3 cut(s) 382, 433, 593
BshVI ATCGAT 1 cut(s) 648
BsiEI CGRYCG 1 cut(s) 652
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 3 cut(s) 178, 500, 594
BslFI GGGAC 2 cut(s) 356, 666
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 4 cut(s) 10, 244, 260, 422
BsmBI CGTCTC 2 cut(s) 10, 244
BsmFI GGGAC 2 cut(s) 356, 666
BsnI GGCC 3 cut(s) 382, 433, 593
Bso31I GGTCTC 1 cut(s) 260
Bsp119I TTCGAA 1 cut(s) 507
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 6 cut(s) 99, 298, 398, 649, 692, 699
BspACI CCGC 5 cut(s) 33, 86, 379, 610, 706
BspANI GGCC 3 cut(s) 382, 433, 593
BspDI ATCGAT 1 cut(s) 648
BspLI GGNNCC 2 cut(s) 96, 444
BspMAI CTGCAG 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 471
BspPI GGATC 1 cut(s) 406
BspT104I TTCGAA 1 cut(s) 507
BspTNI GGTCTC 1 cut(s) 260
BsrFI RCCGGY 2 cut(s) 499, 593
BsrI ACTGG 1 cut(s) 446
BssAI RCCGGY 2 cut(s) 499, 593
BssMI GATC 6 cut(s) 99, 298, 398, 649, 692, 699
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 5 cut(s) 21, 152, 370, 425, 442
BstBI TTCGAA 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 614
BstKTI GATC 6 cut(s) 102, 301, 401, 652, 695, 702
BstMAI GTCTC 4 cut(s) 10, 244, 260, 422
BstMBI GATC 6 cut(s) 99, 298, 398, 649, 692, 699
BstMCI CGRYCG 1 cut(s) 652
BstMWI GCNNNNNNNGC 2 cut(s) 622, 664
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 90, 177
BstSFI CTRYAG 1 cut(s) 552
BstV1I GCAGC 2 cut(s) 42, 538
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 3 cut(s) 382, 433, 593
BsuTUI ATCGAT 1 cut(s) 648
BtrI CACGTC 1 cut(s) 254
BveI ACCTGC 1 cut(s) 471
Cac8I GCNNGC 1 cut(s) 614
Cfr10I RCCGGY 2 cut(s) 499, 593
Cfr13I GGNCC 2 cut(s) 88, 366
ClaI ATCGAT 1 cut(s) 648
CpoI CGGWCCG 1 cut(s) 366
CsiI ACCWGGT 1 cut(s) 90
CspI CGGWCCG 1 cut(s) 366
CviAII CATG 4 cut(s) 80, 146, 564, 635
DpnI GATC 6 cut(s) 101, 300, 400, 651, 694, 701
DpnII GATC 6 cut(s) 99, 298, 398, 649, 692, 699
DraI TTTAAA 1 cut(s) 490
EciI GGCGGA 1 cut(s) 101
Eco147I AGGCCT 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 2 cut(s) 88, 366
EcoRII CCWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 647
Esp3I CGTCTC 2 cut(s) 10, 244
FaeI CATG 4 cut(s) 83, 149, 567, 638
FalI AAGNNNNNCTT 4 cut(s) 303, 335, 519, 551
FaqI GGGAC 2 cut(s) 356, 666
FatI CATG 4 cut(s) 79, 145, 563, 634
FblI GTMKAC 2 cut(s) 9, 169
Fnu4HI GCNGC 5 cut(s) 31, 34, 380, 552, 706
Fsp4HI GCNGC 5 cut(s) 31, 34, 380, 552, 706
FspBI CTAG 4 cut(s) 246, 308, 429, 669
GluI GCNGC 5 cut(s) 31, 34, 380, 552, 706
GsaI CCCAGC 1 cut(s) 231
HaeIII GGCC 3 cut(s) 382, 433, 593
HapII CCGG 3 cut(s) 178, 500, 594
Hin1II CATG 4 cut(s) 83, 149, 567, 638
HincII GTYRAC 2 cut(s) 10, 170
HindII GTYRAC 2 cut(s) 10, 170
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 3 cut(s) 185, 415, 509
HpaII CCGG 3 cut(s) 178, 500, 594
HphI GGTGA 3 cut(s) 167, 452, 489
Hpy166II GTNNAC 2 cut(s) 10, 170
Hpy188I TCNGA 4 cut(s) 76, 99, 104, 699
Hpy188III TCNNGA 4 cut(s) 53, 196, 288, 661
Hpy8I GTNNAC 2 cut(s) 10, 170
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 5 cut(s) 21, 152, 370, 425, 442
HpyCH4IV ACGT 2 cut(s) 253, 405
HpyCH4V TGCA 4 cut(s) 485, 554, 576, 645
HpyF10VI GCNNNNNNNGC 2 cut(s) 622, 664
HpySE526I ACGT 2 cut(s) 253, 405
Hsp92II CATG 4 cut(s) 83, 149, 567, 638
Kzo9I GATC 6 cut(s) 99, 298, 398, 649, 692, 699
Lsp1109I GCAGC 2 cut(s) 42, 538
LweI GCATC 3 cut(s) 585, 632, 654
MabI ACCWGGT 1 cut(s) 90
MaeI CTAG 4 cut(s) 246, 308, 429, 669
MaeII ACGT 2 cut(s) 253, 405
MalI GATC 6 cut(s) 101, 300, 400, 651, 694, 701
MboI GATC 6 cut(s) 99, 298, 398, 649, 692, 699
MboII GAAGA 2 cut(s) 194, 323
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 4 cut(s) 118, 291, 409, 517
MlyI GAGTC 1 cut(s) 424
Mph1103I ATGCAT 1 cut(s) 647
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 3 cut(s) 178, 500, 594
MspR9I CCNGG 2 cut(s) 92, 179
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 2 cut(s) 622, 664
NciI CCSGG 1 cut(s) 179
NdeII GATC 6 cut(s) 99, 298, 398, 649, 692, 699
NlaIII CATG 4 cut(s) 83, 149, 567, 638
NlaIV GGNNCC 2 cut(s) 96, 444
NsiI ATGCAT 1 cut(s) 647
NspV TTCGAA 1 cut(s) 507
PaqCI CACCTGC 1 cut(s) 471
PceI AGGCCT 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 2 cut(s) 185, 509
PkrI GCNGC 5 cut(s) 32, 35, 381, 553, 707
Ple19I CGATCG 1 cut(s) 652
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
PsiI TTATAA 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 90
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 96, 444
PspPI GGNCC 2 cut(s) 88, 366
PstI CTGCAG 1 cut(s) 556
PvuI CGATCG 1 cut(s) 652
Rsr2I CGGWCCG 1 cut(s) 366
RsrII CGGWCCG 1 cut(s) 366
SalI GTCGAC 2 cut(s) 8, 168
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 5 cut(s) 31, 34, 380, 552, 706
Sau3AI GATC 6 cut(s) 99, 298, 398, 649, 692, 699
Sau96I GGNCC 2 cut(s) 88, 366
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 92, 179
SduI GDGCHC 1 cut(s) 261
SexAI ACCWGGT 1 cut(s) 90
SfaNI GCATC 3 cut(s) 585, 632, 654
SfcI CTRYAG 1 cut(s) 552
SfuI TTCGAA 1 cut(s) 507
SinI GGWCC 2 cut(s) 88, 366
SmlI CTYRAG 2 cut(s) 233, 659
SmoI CTYRAG 2 cut(s) 233, 659
Sse9I AATT 4 cut(s) 118, 291, 409, 517
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 5 cut(s) 33, 86, 379, 610, 706
SspMI CTAG 4 cut(s) 246, 308, 429, 669
StuI AGGCCT 1 cut(s) 433
StyD4I CCNGG 2 cut(s) 90, 177
TaaI ACNGT 5 cut(s) 21, 152, 370, 425, 442
TaiI ACGT 2 cut(s) 256, 408
TaqI TCGA 9 cut(s) 9, 169, 322, 401, 450, 507, 579, 648, 691
TasI AATT 4 cut(s) 118, 291, 409, 517
TauI GCSGC 3 cut(s) 36, 382, 708
TfiI GAWTC 2 cut(s) 185, 509
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseI GCWGC 2 cut(s) 30, 551
TspDTI ATGAA 4 cut(s) 17, 231, 375, 651
TspGWI ACGGA 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 88, 366
XapI RAATTY 3 cut(s) 291, 409, 517
XmiI GTMKAC 2 cut(s) 9, 169
XmnI GAANNNNTTC 1 cut(s) 66
XspI CTAG 4 cut(s) 246, 308, 429, 669
Zsp2I ATGCAT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.