RchiOBHm_Chr7g0207341

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0030527
Physical Location & Seq
Reverse (-)
24716501 .. 24716779
279 bp
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UTR
Exon/CDS
Intron
PRQ18556

Sequence Viewer

Length: 279 bp
ATGAGATTGAACTGGAATACTGAGTATGGACAGTTCTTCCTCGAATGGTACTCAGAGAAGCTATTAAGGCCGGGAAATAGACTCCCGGCGGCTGCAAAAGGAGTGTTCCAAGGAACTATAGCTAAACTATCTGGAAAGGTAGCTGGGATTCATTGGCACTACAGAACAAGATCCCATGCTGCTGAATTAACTGCTAGCTACTATAATACATTAATACTAGACTTAGAGATGGTTACAAATCAACAGCAAAAATGTTCAGCAAACATGGGGTTGTATTAA

Protein Analysis

92

Amino Acids

10.57

Weight (kDa)

9.41

Isoelectric Point (pI)

24.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 4 - 72 2.6e-24 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0030527)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0207341
rosa_samantha Rh7DG237900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 89
AclWI GGATC 1 cut(s) 165
AfaI GTAC 1 cut(s) 50
AgsI TTSAA 1 cut(s) 10
AluBI AGCT 4 cut(s) 61, 122, 143, 198
AluI AGCT 4 cut(s) 61, 122, 143, 198
AlwI GGATC 1 cut(s) 165
AoxI GGCC 1 cut(s) 68
ApeKI GCWGC 2 cut(s) 92, 179
AseI ATTAAT 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 72, 86
AsuNHI GCTAGC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 79, 166
BccI CCATC 1 cut(s) 223
BcnI CCSGG 2 cut(s) 72, 86
BfaI CTAG 2 cut(s) 195, 218
BfmI CTRYAG 2 cut(s) 117, 160
BisI GCNGC 3 cut(s) 90, 93, 180
BlsI GCNGC 3 cut(s) 91, 94, 181
Bme1390I CCNGG 2 cut(s) 72, 86
BmrFI CCNGG 2 cut(s) 72, 86
BmtI GCTAGC 1 cut(s) 198
BpuMI CCSGG 2 cut(s) 72, 86
BsaJI CCNNGG 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 12, 66
BseNI ACTGG 1 cut(s) 17
BseXI GCAGC 2 cut(s) 79, 166
BseYI CCCAGC 1 cut(s) 143
BshFI GGCC 1 cut(s) 70
BsiSI CCGG 2 cut(s) 71, 86
BsnI GGCC 1 cut(s) 70
Bsp143I GATC 1 cut(s) 170
BspACI CCGC 1 cut(s) 89
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 2 cut(s) 13, 65
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 165
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 3 cut(s) 21, 52, 223
BstKTI GATC 1 cut(s) 173
BstMBI GATC 1 cut(s) 170
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstSCI CCNGG 2 cut(s) 70, 84
BstSFI CTRYAG 2 cut(s) 117, 160
BstV1I GCAGC 2 cut(s) 79, 166
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
BsuRI GGCC 1 cut(s) 70
Cac8I GCNNGC 1 cut(s) 196
Csp6I GTAC 1 cut(s) 49
CviAII CATG 2 cut(s) 176, 265
CviJI RGCY 6 cut(s) 61, 70, 92, 122, 143, 198
CviKI_1 RGCY 6 cut(s) 61, 70, 92, 122, 143, 198
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 3 cut(s) 21, 52, 223
DpnI GATC 1 cut(s) 172
DpnII GATC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 2 cut(s) 179, 268
FaiI YATR 5 cut(s) 27, 119, 177, 204, 266
FatI CATG 2 cut(s) 175, 264
Fnu4HI GCNGC 3 cut(s) 90, 93, 180
Fsp4HI GCNGC 3 cut(s) 90, 93, 180
FspBI CTAG 2 cut(s) 195, 218
GluI GCNGC 3 cut(s) 90, 93, 180
GsaI CCCAGC 1 cut(s) 147
HaeIII GGCC 1 cut(s) 70
HapII CCGG 2 cut(s) 71, 86
Hin1II CATG 2 cut(s) 179, 268
HinfI GANTC 2 cut(s) 81, 148
HpaII CCGG 2 cut(s) 71, 86
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 95
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 3 cut(s) 21, 52, 223
Hsp92II CATG 2 cut(s) 179, 268
Kzo9I GATC 1 cut(s) 170
LpnPI CCDG 4 cut(s) 84, 99, 117, 129
Lsp1109I GCAGC 2 cut(s) 79, 166
MaeI CTAG 2 cut(s) 195, 218
MaeIII GTNAC 1 cut(s) 232
MalI GATC 1 cut(s) 172
MboI GATC 1 cut(s) 170
MboII GAAGA 1 cut(s) 28
MflI RGATCY 1 cut(s) 170
MluCI AATT 1 cut(s) 185
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 1 cut(s) 50
MseI TTAA 4 cut(s) 65, 188, 212, 277
MspI CCGG 2 cut(s) 71, 86
MspR9I CCNGG 2 cut(s) 72, 86
MwoI GCNNNNNNNGC 1 cut(s) 67
NciI CCSGG 2 cut(s) 72, 86
NdeII GATC 1 cut(s) 170
NheI GCTAGC 1 cut(s) 194
NlaIII CATG 2 cut(s) 179, 268
PfeI GAWTC 1 cut(s) 148
PkrI GCNGC 3 cut(s) 91, 94, 181
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PshBI ATTAAT 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 143
PsrI GAACNNNNNNTAC 2 cut(s) 17, 49
PsuI RGATCY 1 cut(s) 170
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SaqAI TTAA 4 cut(s) 65, 188, 212, 277
SatI GCNGC 3 cut(s) 90, 93, 180
Sau3AI GATC 1 cut(s) 170
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 2 cut(s) 72, 86
SetI ASST 5 cut(s) 63, 124, 141, 145, 200
SfcI CTRYAG 2 cut(s) 117, 160
Sse9I AATT 1 cut(s) 185
SsiI CCGC 1 cut(s) 89
SspMI CTAG 2 cut(s) 195, 218
StyD4I CCNGG 2 cut(s) 70, 84
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 42
TasI AATT 1 cut(s) 185
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 4 cut(s) 65, 188, 212, 277
Tru9I TTAA 4 cut(s) 65, 188, 212, 277
TseI GCWGC 2 cut(s) 92, 179
TspDTI ATGAA 1 cut(s) 140
VspI ATTAAT 1 cut(s) 212
XspI CTAG 2 cut(s) 195, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.