RLG00000023568

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0022697
Physical Location & Seq
Reverse (-)
25272698 .. 25276028
3331 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023568

Sequence Viewer

Length: 1182 bp
ATGTCGTTACTTACAAGGCTCTTGCACAGCATCCAGTGTTACAAAATAGTTAAAAGTGCAATCCAAGTCACTCCTGATCTTCATAGGAAAAGTAAAGGTGGTCTTGAGACCAAAGCATCTTTTAGTGCAACCTTTGTGCATAATATCGCTCCCAGAACTAATCCGGGTGCTGAAGGCTTGGCCTGTATCTTAATCAGTCATAGTCTTCCTGAGAAGAGTGATGGAAAATGGCTTGGAATTAAGCGATTACACAGAGACAGTCAATATGACAGCCAAAGCCAGAATATCACTGTCCTTATTTCCTGCACCAATAAAACTGTGGAGGAAATGAAGAATCCAGTTTTGTCTCTCCCTCTCGATCTCTCAGCTTATCTTCCAGAGAAGGAGTTACTCCTTACTTGGACTGGAAAACAAAAAGTTAAAAGGCGCTACTGGAGGATGCATATCCAAGGATTGAAGAAGAGATTCAAAGCAATTCTATGGTTCCAAAGAAATTCAAACCAAAAGAACTTCAGACAGCAACAGAAGAATTTCATCTTAAGAACAAGCTTGGACAAGATTCTATTCTGGGATGAGAATTTGCTCAAGGGAATAGAACAATCAACTCTGAGCTGGGAAAGAAGGCACACCATAATAGGTAGGGTTGCTCAATCGTTAGATTATCTTCACACTGGATGCGAGAAGAAAGTACATCACCGGGACATAAAAGCCAGCAATATCATGTTAGACTTGGATTTCATCCCCAGGCTGGGAGACTTTGGACTGGCTCGAACCATTCAGCAAAGTGAGTTAACTCACCACTCCACCAAAGAGATTGCAGGAATACCAGGTTATATGGCTCCAGAAACATTTCTAACAGGAAGGGCTACTGTTGAAACAGATGTTTATGCATTTGGTGTTCTTGGAACGATCCTTGATGTTGTCGACTCGAGATTGGATAGAGATATTGGTAAGGATGATATGGCATGTGTGCTGATTCTGGGATTAGCATGCTGCCATCCTAACCCACATCAGAAGCCTTCCTTGAGAACTGTCATTAAGGTTCTTACAAGGGAAGCAGATCCACTATTGTCGCCCACCGAAGGACCTGCTTTTGTGTGGCCAGCCATGCCTCCATCATTCCAAGAGACAACTTTGGATGGAAGTCAGATCACTACATTCACAGAACTCTCTGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

394

Amino Acids

44.5

Weight (kDa)

9.33

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 191 - 301 5.6e-18 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 199 - 301 4.8e-19 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022697)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479481
rosa_laevigata RLG00000023568
rosa_roxburghii Rroxscaffold_6G00402210
rosa_rugosa Rorug03G0175600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1096
AccI GTMKAC 1 cut(s) 924
AclWI GGATC 2 cut(s) 904, 1055
AcoI YGGCCR 1 cut(s) 1100
AcsI RAATTY 3 cut(s) 493, 529, 577
AcuI CTGAAG 2 cut(s) 192, 496
AfaI GTAC 1 cut(s) 690
AfiI CCNNNNNNNGG 3 cut(s) 748, 749, 1082
AflII CTTAAG 1 cut(s) 538
AgsI TTSAA 4 cut(s) 457, 469, 498, 875
AjnI CCWGG 2 cut(s) 743, 826
AluBI AGCT 3 cut(s) 368, 549, 612
AluI AGCT 3 cut(s) 368, 549, 612
Alw26I GTCTC 5 cut(s) 101, 249, 351, 747, 1121
AlwI GGATC 2 cut(s) 904, 1055
Ama87I CYCGRG 1 cut(s) 928
AoxI GGCC 2 cut(s) 180, 1100
ApeKI GCWGC 1 cut(s) 993
ApoI RAATTY 3 cut(s) 493, 529, 577
Asp700I GAANNNNTTC 3 cut(s) 464, 530, 849
AspLEI GCGC 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 1085
AsuC2I CCSGG 2 cut(s) 165, 698
AsuHPI GGTGA 2 cut(s) 686, 788
AvaI CYCGRG 1 cut(s) 928
AvaII GGWCC 1 cut(s) 1085
BalI TGGCCA 1 cut(s) 1102
BbsI GAAGAC 1 cut(s) 197
BbvI GCAGC 1 cut(s) 980
BccI CCATC 4 cut(s) 215, 1005, 1123, 1133
BcgI CGANNNNNNTGC 2 cut(s) 1070, 1104
BciT130I CCWGG 2 cut(s) 745, 828
BcnI CCSGG 2 cut(s) 165, 698
BcoDI GTCTC 5 cut(s) 101, 249, 351, 747, 1121
BfoI RGCGCY 1 cut(s) 430
BfrI CTTAAG 1 cut(s) 538
BfuAI ACCTGC 1 cut(s) 1096
BisI GCNGC 1 cut(s) 994
BlsI GCNGC 1 cut(s) 995
Bme1390I CCNGG 4 cut(s) 165, 698, 745, 828
Bme18I GGWCC 1 cut(s) 1085
BmeT110I CYCGRG 1 cut(s) 928
BmgT120I GGNCC 1 cut(s) 1085
BmiI GGNNCC 2 cut(s) 485, 840
BmrFI CCNGG 4 cut(s) 165, 698, 745, 828
BmsI GCATC 4 cut(s) 39, 125, 429, 665
BpiI GAAGAC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 454, 825
BpuEI CTTGAG 3 cut(s) 125, 569, 1045
BpuMI CCSGG 2 cut(s) 165, 698
BsaBI GATNNNNATC 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 448, 743
Bsc4I CCNNNNNNNGG 3 cut(s) 748, 749, 1082
Bse1I ACTGG 6 cut(s) 34, 338, 409, 437, 676, 768
Bse8I GATNNNNATC 1 cut(s) 443
BseBI CCWGG 2 cut(s) 745, 828
BseDI CCNNGG 2 cut(s) 448, 743
BseGI GGATG 8 cut(s) 30, 444, 577, 680, 738, 961, 997, 1144
BseJI GATNNNNATC 1 cut(s) 443
BseLI CCNNNNNNNGG 3 cut(s) 748, 749, 1082
BseMII CTCAG 3 cut(s) 201, 378, 599
BseNI ACTGG 6 cut(s) 34, 338, 409, 437, 676, 768
BseXI GCAGC 1 cut(s) 980
BseYI CCCAGC 2 cut(s) 612, 748
BsgI GTGCAG 1 cut(s) 289
BshFI GGCC 2 cut(s) 182, 1102
BsiHKCI CYCGRG 1 cut(s) 928
BsiSI CCGG 2 cut(s) 164, 697
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 3 cut(s) 748, 749, 1082
BsmAI GTCTC 5 cut(s) 101, 249, 351, 747, 1121
BsmFI GGGAC 1 cut(s) 713
BsnI GGCC 2 cut(s) 182, 1102
Bso31I GGTCTC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 928
Bsp143I GATC 5 cut(s) 76, 358, 909, 1060, 1149
BspANI GGCC 2 cut(s) 182, 1102
BspCNI CTCAG 3 cut(s) 202, 377, 600
BspLI GGNNCC 2 cut(s) 485, 840
BspMI ACCTGC 1 cut(s) 1096
BspPI GGATC 2 cut(s) 904, 1055
BspTI CTTAAG 1 cut(s) 538
BspTNI GGTCTC 1 cut(s) 101
BsrI ACTGG 6 cut(s) 34, 338, 409, 437, 676, 768
BssECI CCNNGG 2 cut(s) 448, 743
BssMI GATC 5 cut(s) 76, 358, 909, 1060, 1149
BssT1I CCWWGG 1 cut(s) 448
Bst2UI CCWGG 2 cut(s) 745, 828
Bst4CI ACNGT 5 cut(s) 260, 292, 319, 871, 1033
Bst6I CTCTTC 2 cut(s) 209, 455
BstAFI CTTAAG 1 cut(s) 538
BstC8I GCNNGC 3 cut(s) 712, 991, 1104
BstDEI CTNAG 3 cut(s) 210, 364, 608
BstF5I GGATG 8 cut(s) 30, 444, 577, 680, 738, 961, 997, 1144
BstH2I RGCGCY 1 cut(s) 430
BstHHI GCGC 1 cut(s) 429
BstKTI GATC 5 cut(s) 79, 361, 912, 1063, 1152
BstMAI GTCTC 5 cut(s) 101, 249, 351, 747, 1121
BstMBI GATC 5 cut(s) 76, 358, 909, 1060, 1149
BstMWI GCNNNNNNNGC 1 cut(s) 1108
BstNI CCWGG 2 cut(s) 745, 828
BstNSI RCATGY 2 cut(s) 969, 993
BstSCI CCNGG 4 cut(s) 163, 696, 743, 826
BstV1I GCAGC 1 cut(s) 980
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 1060
BsuRI GGCC 2 cut(s) 182, 1102
BtsCI GGATG 8 cut(s) 30, 444, 577, 680, 738, 961, 997, 1144
BtsIMutI CAGTG 3 cut(s) 41, 288, 669
BveI ACCTGC 1 cut(s) 1096
Cac8I GCNNGC 3 cut(s) 712, 991, 1104
CfoI GCGC 1 cut(s) 429
Cfr13I GGNCC 1 cut(s) 1085
CsiI ACCWGGT 1 cut(s) 826
Csp6I GTAC 1 cut(s) 689
CviAII CATG 4 cut(s) 721, 966, 990, 1108
CviQI GTAC 1 cut(s) 689
DdeI CTNAG 3 cut(s) 210, 364, 608
DpnI GATC 5 cut(s) 78, 360, 911, 1062, 1151
DpnII GATC 5 cut(s) 76, 358, 909, 1060, 1149
EaeI YGGCCR 1 cut(s) 1100
Eam1104I CTCTTC 2 cut(s) 209, 455
EarI CTCTTC 2 cut(s) 209, 455
Eco130I CCWWGG 1 cut(s) 448
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 1085
Eco57I CTGAAG 2 cut(s) 192, 496
Eco88I CYCGRG 1 cut(s) 928
EcoO109I RGGNCCY 1 cut(s) 1085
EcoRII CCWGG 2 cut(s) 743, 826
EcoT14I CCWWGG 1 cut(s) 448
EcoT22I ATGCAT 2 cut(s) 444, 892
ErhI CCWWGG 1 cut(s) 448
FaeI CATG 4 cut(s) 724, 969, 993, 1111
FalI AAGNNNNNCTT 4 cut(s) 87, 119, 1007, 1039
FaqI GGGAC 1 cut(s) 713
FatI CATG 4 cut(s) 720, 965, 989, 1107
FblI GTMKAC 1 cut(s) 924
Fnu4HI GCNGC 1 cut(s) 994
FokI GGATG 8 cut(s) 17, 451, 584, 687, 725, 968, 984, 1151
Fsp4HI GCNGC 1 cut(s) 994
GlaI GCGC 1 cut(s) 428
GluI GCNGC 1 cut(s) 994
GsaI CCCAGC 2 cut(s) 616, 752
GsuI CTGGAG 2 cut(s) 454, 825
HaeII RGCGCY 1 cut(s) 430
HaeIII GGCC 2 cut(s) 182, 1102
HapII CCGG 2 cut(s) 164, 697
HhaI GCGC 1 cut(s) 429
Hin1II CATG 4 cut(s) 724, 969, 993, 1111
Hin6I GCGC 1 cut(s) 427
HinP1I GCGC 1 cut(s) 427
HincII GTYRAC 2 cut(s) 792, 925
HindII GTYRAC 2 cut(s) 792, 925
HindIII AAGCTT 1 cut(s) 547
HinfI GANTC 5 cut(s) 334, 465, 559, 926, 976
HpaI GTTAAC 1 cut(s) 792
HpaII CCGG 2 cut(s) 164, 697
HphI GGTGA 2 cut(s) 686, 788
Hpy166II GTNNAC 2 cut(s) 792, 925
Hpy188I TCNGA 4 cut(s) 515, 609, 1014, 1149
Hpy188III TCNNGA 8 cut(s) 74, 104, 209, 356, 377, 842, 930, 1173
Hpy8I GTNNAC 2 cut(s) 792, 925
HpyAV CCTTC 6 cut(s) 167, 376, 615, 855, 1029, 1076
HpyCH4III ACNGT 5 cut(s) 260, 292, 319, 871, 1033
HpyCH4V TGCA 8 cut(s) 25, 59, 128, 139, 306, 442, 818, 890
HpyF10VI GCNNNNNNNGC 1 cut(s) 1108
HpyF3I CTNAG 3 cut(s) 210, 364, 608
Hsp92II CATG 4 cut(s) 724, 969, 993, 1111
HspAI GCGC 1 cut(s) 427
KspAI GTTAAC 1 cut(s) 792
Kzo9I GATC 5 cut(s) 76, 358, 909, 1060, 1149
LmnI GCTCC 2 cut(s) 154, 844
Lsp1109I GCAGC 1 cut(s) 980
LweI GCATC 4 cut(s) 39, 125, 429, 665
MabI ACCWGGT 1 cut(s) 826
MaeIII GTNAC 4 cut(s) 6, 38, 67, 387
MalI GATC 5 cut(s) 78, 360, 911, 1062, 1151
MboI GATC 5 cut(s) 76, 358, 909, 1060, 1149
MflI RGATCY 1 cut(s) 1060
MlsI TGGCCA 1 cut(s) 1102
MluCI AATT 5 cut(s) 237, 474, 493, 529, 577
MluNI TGGCCA 1 cut(s) 1102
MlyI GAGTC 1 cut(s) 920
MnlI CCTC 4 cut(s) 316, 363, 429, 1122
Mox20I TGGCCA 1 cut(s) 1102
Mph1103I ATGCAT 2 cut(s) 444, 892
MroXI GAANNNNTTC 3 cut(s) 464, 530, 849
MscI TGGCCA 1 cut(s) 1102
MseI TTAA 7 cut(s) 51, 191, 240, 420, 539, 791, 1038
Msp20I TGGCCA 1 cut(s) 1102
MspCI CTTAAG 1 cut(s) 538
MspI CCGG 2 cut(s) 164, 697
MspR9I CCNGG 4 cut(s) 165, 698, 745, 828
MvaI CCWGG 2 cut(s) 745, 828
MwoI GCNNNNNNNGC 1 cut(s) 1108
NciI CCSGG 2 cut(s) 165, 698
NdeII GATC 5 cut(s) 76, 358, 909, 1060, 1149
NlaIII CATG 4 cut(s) 724, 969, 993, 1111
NlaIV GGNNCC 2 cut(s) 485, 840
NmuCI GTSAC 1 cut(s) 67
NsiI ATGCAT 2 cut(s) 444, 892
NspI RCATGY 2 cut(s) 969, 993
PaeI GCATGC 1 cut(s) 993
PaeR7I CTCGAG 1 cut(s) 928
PdmI GAANNNNTTC 3 cut(s) 464, 530, 849
PfeI GAWTC 4 cut(s) 334, 465, 559, 976
PkrI GCNGC 1 cut(s) 995
PleI GAGTC 1 cut(s) 920
PpsI GAGTC 1 cut(s) 920
PpuMI RGGWCCY 1 cut(s) 1085
Psp5II RGGWCCY 1 cut(s) 1085
Psp6I CCWGG 2 cut(s) 743, 826
PspFI CCCAGC 2 cut(s) 612, 748
PspGI CCWGG 2 cut(s) 743, 826
PspN4I GGNNCC 2 cut(s) 485, 840
PspPI GGNCC 1 cut(s) 1085
PspPPI RGGWCCY 1 cut(s) 1085
PsuI RGATCY 1 cut(s) 1060
RsaI GTAC 1 cut(s) 690
RsaNI GTAC 1 cut(s) 689
SalI GTCGAC 1 cut(s) 923
SaqAI TTAA 7 cut(s) 51, 191, 240, 420, 539, 791, 1038
SatI GCNGC 1 cut(s) 994
Sau3AI GATC 5 cut(s) 76, 358, 909, 1060, 1149
Sau96I GGNCC 1 cut(s) 1085
SchI GAGTC 1 cut(s) 920
ScrFI CCNGG 4 cut(s) 165, 698, 745, 828
SetI ASST 9 cut(s) 100, 134, 370, 551, 614, 640, 832, 1044, 1090
SexAI ACCWGGT 1 cut(s) 826
SfaNI GCATC 4 cut(s) 39, 125, 429, 665
Sfr274I CTCGAG 1 cut(s) 928
SinI GGWCC 1 cut(s) 1085
SlaI CTCGAG 1 cut(s) 928
SmlI CTYRAG 5 cut(s) 104, 538, 584, 928, 1024
SmoI CTYRAG 5 cut(s) 104, 538, 584, 928, 1024
SphI GCATGC 1 cut(s) 993
Sse9I AATT 5 cut(s) 237, 474, 493, 529, 577
StyD4I CCNGG 4 cut(s) 163, 696, 743, 826
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 5 cut(s) 260, 292, 319, 871, 1033
TaqI TCGA 4 cut(s) 357, 769, 924, 929
TasI AATT 5 cut(s) 237, 474, 493, 529, 577
TatI WGTACW 1 cut(s) 688
TfiI GAWTC 4 cut(s) 334, 465, 559, 976
Tru1I TTAA 7 cut(s) 51, 191, 240, 420, 539, 791, 1038
Tru9I TTAA 7 cut(s) 51, 191, 240, 420, 539, 791, 1038
TscAI CASTG 3 cut(s) 41, 295, 676
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 1 cut(s) 993
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 4 cut(s) 71, 344, 523, 727
TspRI CASTG 3 cut(s) 41, 295, 676
Vha464I CTTAAG 1 cut(s) 538
VpaK11BI GGWCC 1 cut(s) 1085
XapI RAATTY 3 cut(s) 493, 529, 577
XceI RCATGY 2 cut(s) 969, 993
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XhoI CTCGAG 1 cut(s) 928
XmiI GTMKAC 1 cut(s) 924
XmnI GAANNNNTTC 3 cut(s) 464, 530, 849
Zsp2I ATGCAT 2 cut(s) 444, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.