RLG00000023655

Amidohydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0024789
Physical Location & Seq
Reverse (-)
26669322 .. 26671528
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023655

Sequence Viewer

Length: 378 bp
ATGAAAAACCCAGTCCAGACTATCTGCTGCAACAATGGCTGTAGGGGAGCCCAGAGCACCAAAGTAATAAATTCACATCTCCATGTTTGGGCTTTTCCTCAGAAGGCTGCTGGTAAATATCCTTACTTTCCTGGCCCAGAACCCACTTTGCCTGGGGATATCGATTTGTTGCTTCAGGTTTGTAGAGAAAACAACAACCATGGGTCTAGAAGAATGCTATTCACATCGAACTCTAAGCCGGCCGAGATCTACTCCTGTTATTTATTTCTGCTGGTATATATCTTTACTTTCCTGGCCAAGAACCCACTTTGCCTGGGGATATCAATTTGTTGCTTCAGGTATGGCTTTCATGAACTTCCCTATGTGTTTCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_2

Protein Analysis

126

Amino Acids

14.15

Weight (kDa)

8.82

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024789)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0319171 RchiOBHm_Chr2g0135531
rosa_laevigata RLG00000023655

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 240, 294
AcsI RAATTY 1 cut(s) 70
AcuI CTGAAG 2 cut(s) 158, 319
AfiI CCNNNNNNNGG 1 cut(s) 88
AjnI CCWGG 4 cut(s) 130, 151, 291, 312
Alw21I GWGCWC 1 cut(s) 59
AoxI GGCC 3 cut(s) 133, 240, 294
ApeKI GCWGC 2 cut(s) 27, 107
ApoI RAATTY 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 134
BalI TGGCCA 1 cut(s) 296
BanII GRGCYC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 2 cut(s) 14, 94
BciT130I CCWGG 4 cut(s) 132, 153, 293, 314
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 1 cut(s) 40
BglII AGATCT 1 cut(s) 246
BisI GCNGC 2 cut(s) 28, 108
BlsI GCNGC 2 cut(s) 29, 109
Bme1390I CCNGG 4 cut(s) 132, 153, 293, 314
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 4 cut(s) 132, 153, 293, 314
BmrI ACTGGG 1 cut(s) 5
BmuI ACTGGG 1 cut(s) 5
BplI GAGNNNNNCTC 2 cut(s) 236, 268
Bsa29I ATCGAT 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 152, 199, 313
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 1 cut(s) 11
BseBI CCWGG 4 cut(s) 132, 153, 293, 314
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 152, 199, 313
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 11
BseX3I CGGCCG 1 cut(s) 240
BseXI GCAGC 2 cut(s) 14, 94
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 3 cut(s) 135, 242, 296
BshVI ATCGAT 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 88
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 3 cut(s) 135, 242, 296
Bsp1286I GDGCHC 2 cut(s) 52, 59
Bsp143I GATC 1 cut(s) 246
Bsp19I CCATGG 1 cut(s) 199
BspANI GGCC 3 cut(s) 135, 242, 296
BspCNI CTCAG 1 cut(s) 112
BspDI ATCGAT 1 cut(s) 162
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 49
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 3 cut(s) 152, 199, 313
BssMI GATC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 199
Bst2UI CCWGG 4 cut(s) 132, 153, 293, 314
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 2 cut(s) 99, 234
BstDSI CCRYGG 1 cut(s) 199
BstKTI GATC 1 cut(s) 249
BstMBI GATC 1 cut(s) 246
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstNI CCWGG 4 cut(s) 132, 153, 293, 314
BstSCI CCNGG 4 cut(s) 130, 151, 291, 312
BstSFI CTRYAG 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 14, 94
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BstZI CGGCCG 1 cut(s) 240
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 3 cut(s) 135, 242, 296
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 240
CciI TCATGA 1 cut(s) 349
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 162
CviAII CATG 3 cut(s) 83, 200, 350
CviJI RGCY 9 cut(s) 39, 50, 92, 107, 135, 238, 242, 296, 345
CviKI_1 RGCY 9 cut(s) 39, 50, 92, 107, 135, 238, 242, 296, 345
DdeI CTNAG 2 cut(s) 99, 234
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
EaeI YGGCCR 2 cut(s) 240, 294
EagI CGGCCG 1 cut(s) 240
EclXI CGGCCG 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 52
Eco32I GATATC 2 cut(s) 160, 321
Eco52I CGGCCG 1 cut(s) 240
Eco57I CTGAAG 2 cut(s) 158, 319
EcoRII CCWGG 4 cut(s) 130, 151, 291, 312
EcoRV GATATC 2 cut(s) 160, 321
EcoT14I CCWWGG 1 cut(s) 199
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 199
FaeI CATG 3 cut(s) 86, 203, 353
FaiI YATR 7 cut(s) 84, 201, 277, 279, 342, 351, 363
FatI CATG 3 cut(s) 82, 199, 349
Fnu4HI GCNGC 2 cut(s) 28, 108
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 2 cut(s) 28, 108
FspBI CTAG 1 cut(s) 207
GluI GCNGC 2 cut(s) 28, 108
HaeIII GGCC 3 cut(s) 135, 242, 296
HapII CCGG 1 cut(s) 239
Hin1II CATG 3 cut(s) 86, 203, 353
HpaII CCGG 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 102
Hpy188III TCNNGA 3 cut(s) 16, 207, 350
HpyAV CCTTC 1 cut(s) 97
HpyCH4V TGCA 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 2 cut(s) 99, 234
Hsp92II CATG 3 cut(s) 86, 203, 353
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 2 cut(s) 14, 94
MaeI CTAG 1 cut(s) 207
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 1 cut(s) 222
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 2 cut(s) 52, 59
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 2 cut(s) 70, 324
MluNI TGGCCA 1 cut(s) 296
MnlI CCTC 1 cut(s) 108
Mox20I TGGCCA 1 cut(s) 296
MroNI GCCGGC 1 cut(s) 238
MscI TGGCCA 1 cut(s) 296
MseI TTAA 1 cut(s) 376
MslI CAYNNNNRTG 1 cut(s) 81
Msp20I TGGCCA 1 cut(s) 296
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 4 cut(s) 132, 153, 293, 314
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 4 cut(s) 132, 153, 293, 314
MwoI GCNNNNNNNGC 1 cut(s) 36
NaeI GCCGGC 1 cut(s) 240
NcoI CCATGG 1 cut(s) 199
NdeII GATC 1 cut(s) 246
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 3 cut(s) 86, 203, 353
NlaIV GGNNCC 1 cut(s) 49
NmeAIII GCCGAG 1 cut(s) 268
PagI TCATGA 1 cut(s) 349
PctI GAATGC 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 240
PkrI GCNGC 2 cut(s) 29, 109
Psp6I CCWGG 4 cut(s) 130, 151, 291, 312
PspGI CCWGG 4 cut(s) 130, 151, 291, 312
PspN4I GGNNCC 1 cut(s) 49
PspPI GGNCC 1 cut(s) 134
PsuI RGATCY 1 cut(s) 246
RseI CAYNNNNRTG 1 cut(s) 81
SaqAI TTAA 1 cut(s) 376
SatI GCNGC 2 cut(s) 28, 108
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 1 cut(s) 134
ScrFI CCNGG 4 cut(s) 132, 153, 293, 314
SduI GDGCHC 2 cut(s) 52, 59
SetI ASST 2 cut(s) 180, 341
SfcI CTRYAG 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 81
Sse9I AATT 2 cut(s) 70, 324
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 4 cut(s) 130, 151, 291, 312
StyI CCWWGG 1 cut(s) 199
TaqI TCGA 2 cut(s) 162, 227
TasI AATT 2 cut(s) 70, 324
Tru1I TTAA 1 cut(s) 376
Tru9I TTAA 1 cut(s) 376
TseI GCWGC 2 cut(s) 27, 107
TspDTI ATGAA 3 cut(s) 17, 338, 366
XapI RAATTY 1 cut(s) 70
XbaI TCTAGA 1 cut(s) 206
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.