RLG00000027313
HSP70 Family

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0028132
Physical Location & Seq
Reverse (-)
10512636 .. 10514081
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027313

Sequence Viewer

Length: 780 bp
ATGGCTAGAGAAGAAACTGTACATGCTATTGGGATTGATTTGGGAACTACAAATTCGTGTGTTGCAGTATGGCAAAATGATCATGTCGAAATCATAATAAACGATCTGGGGATGAGGACCACCCCTTCTATTGTTGCCTTCAGGGGTAGTCAGAAGTTAGTAGGTGATGCAGCCATTTACCAACTCGAGAGAAATCCCACAAATTCTGTCTTCGATGCCAAGCAGTTGATAGGCAGGAGATTCAGTGACGCATCAGTTCAAAATGATATAAAGCTTTGGCCATTCAAAGTAACTGAAGGTCCTGGTGACAAGCCCAAGATCGTGGTTACCCATGAGGATCAGGAGAAACAGTTTTCTGCTGAAGATATCTCATCCATGGTTCTCACAAAGATGCGTGGTATCGCTGAAGCGTATATCGGCACAACTGTGAAGAATGCAGTAATCACTGTCCCTGCTTTTTTCAATGACTCACAGAGACAAGCTTCAAAAACTGCTGGTGCTGCAGCAGGACTAAATGTGAAGTGTATTATCAATGAACCAACTGTTGCAGCCATAGCCTATGGGCTCGTTAGGAAGGCTGGCTGGTATAGCAAGAGAAATGTGATGGTGTTTGATATGGGTGGTGGTACTTTGGATGTTTCATTGCTTACCATAGGTGATGGTGCCTGTGAAGTGAAGACCACTGCTGGACACACCCACCTCGGTTGTGAAGACTTTGATAACAAAATGCTGAGTTTTTGTGTTGAGGAATTTAAGAGGGAGCACAATTTGGACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

260

Amino Acids

28.25

Weight (kDa)

5.71

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 259 3.2e-101 Hsp70 protein
MreB_Mbl PF06723 142 - 251 1.2e-07 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028132)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g22330
rosa_laevigata RLG00000027313

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 662
AclWI GGATC 1 cut(s) 345
AcoI YGGCCR 1 cut(s) 278
AcsI RAATTY 3 cut(s) 52, 202, 749
AcuI CTGAAG 4 cut(s) 124, 315, 381, 426
AfaI GTAC 2 cut(s) 21, 628
AgsI TTSAA 4 cut(s) 260, 286, 463, 486
AjnI CCWGG 1 cut(s) 301
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 2 cut(s) 274, 482
AluI AGCT 2 cut(s) 274, 482
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 1 cut(s) 345
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 4 cut(s) 170, 500, 503, 548
ApoI RAATTY 3 cut(s) 52, 202, 749
AspS9I GGNCC 2 cut(s) 117, 299
AsuHPI GGTGA 3 cut(s) 176, 317, 668
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 2 cut(s) 117, 299
BalI TGGCCA 1 cut(s) 280
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 567
BarI GAAGNNNNNNTAC 1 cut(s) 35
BbsI GAAGAC 3 cut(s) 202, 683, 717
Bbv12I GWGCWC 1 cut(s) 765
BbvI GCAGC 4 cut(s) 182, 487, 515, 560
BccI CCATC 2 cut(s) 598, 653
BciT130I CCWGG 1 cut(s) 303
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 469
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 501
BisI GCNGC 4 cut(s) 171, 501, 504, 549
BlsI GCNGC 4 cut(s) 172, 502, 505, 550
Bme1390I CCNGG 1 cut(s) 303
Bme18I GGWCC 2 cut(s) 117, 299
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 2 cut(s) 117, 299
BmiI GGNNCC 1 cut(s) 664
BmrFI CCNGG 1 cut(s) 303
BmsI GCATC 4 cut(s) 157, 205, 260, 381
BpiI GAAGAC 3 cut(s) 202, 683, 717
BsaJI CCNNGG 2 cut(s) 375, 700
BsaXI ACNNNNNCTCC 4 cut(s) 229, 259, 335, 365
Bse3DI GCAATG 1 cut(s) 641
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 375, 700
BseGI GGATG 3 cut(s) 117, 371, 640
BseMI GCAATG 1 cut(s) 641
BseMII CTCAG 1 cut(s) 722
BseXI GCAGC 4 cut(s) 182, 487, 515, 560
BshFI GGCC 1 cut(s) 280
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 765
BsiHKCI CYCGRG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 434
BsmAI GTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 434
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 1 cut(s) 280
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 567, 765
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 4 cut(s) 79, 103, 318, 337
Bsp19I CCATGG 1 cut(s) 375
BspANI GGCC 1 cut(s) 280
BspCNI CTCAG 1 cut(s) 723
BspLI GGNNCC 1 cut(s) 664
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 1 cut(s) 345
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 641
BsrGI TGTACA 1 cut(s) 19
BssECI CCNNGG 2 cut(s) 375, 700
BssMI GATC 4 cut(s) 79, 103, 318, 337
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 5 cut(s) 19, 351, 427, 448, 544
BstAUI TGTACA 1 cut(s) 19
BstC8I GCNNGC 1 cut(s) 580
BstDEI CTNAG 1 cut(s) 731
BstDSI CCRYGG 1 cut(s) 375
BstEII GGTNACC 1 cut(s) 325
BstF5I GGATG 3 cut(s) 117, 371, 640
BstKTI GATC 4 cut(s) 82, 106, 321, 340
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 4 cut(s) 79, 103, 318, 337
BstMWI GCNNNNNNNGC 3 cut(s) 500, 554, 588
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 1 cut(s) 26
BstPI GGTNACC 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 301
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 4 cut(s) 182, 487, 515, 560
BstV2I GAAGAC 3 cut(s) 202, 683, 717
BstXI CCANNNNNNTGG 1 cut(s) 322
BsuRI GGCC 1 cut(s) 280
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 3 cut(s) 117, 371, 640
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 3 cut(s) 250, 444, 681
Cac8I GCNNGC 1 cut(s) 580
Cfr13I GGNCC 2 cut(s) 117, 299
CseI GACGC 1 cut(s) 257
Csp6I GTAC 2 cut(s) 20, 627
CspCI CAANNNNNGTGG 2 cut(s) 686, 721
CviAII CATG 4 cut(s) 23, 83, 332, 376
CviQI GTAC 2 cut(s) 20, 627
DdeI CTNAG 1 cut(s) 731
DpnI GATC 4 cut(s) 81, 105, 320, 339
DpnII GATC 4 cut(s) 79, 103, 318, 337
EaeI YGGCCR 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 567
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 2 cut(s) 117, 299
Eco57I CTGAAG 4 cut(s) 124, 315, 381, 426
Eco88I CYCGRG 1 cut(s) 185
Eco91I GGTNACC 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 301
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 4 cut(s) 26, 86, 335, 379
FaqI GGGAC 1 cut(s) 434
FatI CATG 4 cut(s) 22, 82, 331, 375
FauNDI CATATG 1 cut(s) 776
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 4 cut(s) 171, 501, 504, 549
FokI GGATG 3 cut(s) 124, 358, 647
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 4 cut(s) 171, 501, 504, 549
FspBI CTAG 1 cut(s) 6
GluI GCNGC 4 cut(s) 171, 501, 504, 549
HaeIII GGCC 1 cut(s) 280
HgaI GACGC 1 cut(s) 257
Hin1II CATG 4 cut(s) 26, 86, 335, 379
HindIII AAGCTT 2 cut(s) 272, 480
HinfI GANTC 2 cut(s) 240, 467
HphI GGTGA 3 cut(s) 176, 317, 668
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 2 cut(s) 187, 341
HpyAV CCTTC 4 cut(s) 135, 148, 290, 568
HpyCH4III ACNGT 5 cut(s) 19, 351, 427, 448, 544
HpyCH4V TGCA 5 cut(s) 65, 170, 437, 503, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 554, 588
HpyF3I CTNAG 1 cut(s) 731
Hsp92II CATG 4 cut(s) 26, 86, 335, 379
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 4 cut(s) 79, 103, 318, 337
LmnI GCTCC 1 cut(s) 760
Lsp1109I GCAGC 4 cut(s) 182, 487, 515, 560
LweI GCATC 4 cut(s) 157, 205, 260, 381
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 4 cut(s) 245, 289, 305, 325
MalI GATC 4 cut(s) 81, 105, 320, 339
MboI GATC 4 cut(s) 79, 103, 318, 337
MboII GAAGA 6 cut(s) 23, 202, 374, 442, 688, 722
MhlI GDGCHC 2 cut(s) 567, 765
MlsI TGGCCA 1 cut(s) 280
MluCI AATT 4 cut(s) 52, 202, 749, 766
MluNI TGGCCA 1 cut(s) 280
MlyI GAGTC 1 cut(s) 461
MnlI CCTC 5 cut(s) 108, 328, 710, 739, 750
Mox20I TGGCCA 1 cut(s) 280
MscI TGGCCA 1 cut(s) 280
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 2 cut(s) 389, 425
Msp20I TGGCCA 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 303
Mva1269I GAATGC 1 cut(s) 439
MvaI CCWGG 1 cut(s) 303
MwoI GCNNNNNNNGC 3 cut(s) 500, 554, 588
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 1 cut(s) 776
NdeII GATC 4 cut(s) 79, 103, 318, 337
NlaIII CATG 4 cut(s) 26, 86, 335, 379
NlaIV GGNNCC 1 cut(s) 664
NmuCI GTSAC 2 cut(s) 245, 305
NspI RCATGY 1 cut(s) 26
OliI CACNNNNGTG 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 185
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 1 cut(s) 240
PkrI GCNGC 4 cut(s) 172, 502, 505, 550
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PpuMI RGGWCCY 1 cut(s) 299
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 301
PspEI GGTNACC 1 cut(s) 325
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 664
PspPI GGNCC 2 cut(s) 117, 299
PspPPI RGGWCCY 1 cut(s) 299
PstI CTGCAG 1 cut(s) 505
RsaI GTAC 2 cut(s) 21, 628
RsaNI GTAC 2 cut(s) 20, 627
RseI CAYNNNNRTG 2 cut(s) 389, 425
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 4 cut(s) 171, 501, 504, 549
Sau3AI GATC 4 cut(s) 79, 103, 318, 337
Sau96I GGNCC 2 cut(s) 117, 299
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 2 cut(s) 567, 765
SetI ASST 6 cut(s) 166, 276, 301, 484, 658, 702
SfaNI GCATC 4 cut(s) 157, 205, 260, 381
SfcI CTRYAG 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 185
SinI GGWCC 2 cut(s) 117, 299
SlaI CTCGAG 1 cut(s) 185
SmiMI CAYNNNNRTG 2 cut(s) 389, 425
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 4 cut(s) 52, 202, 749, 766
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 301
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 5 cut(s) 19, 351, 427, 448, 544
TaqI TCGA 3 cut(s) 87, 186, 213
TasI AATT 4 cut(s) 52, 202, 749, 766
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 3 cut(s) 250, 451, 688
TseFI GTSAC 2 cut(s) 245, 305
TseI GCWGC 4 cut(s) 170, 500, 503, 548
Tsp45I GTSAC 2 cut(s) 245, 305
TspDTI ATGAA 2 cut(s) 549, 630
TspRI CASTG 3 cut(s) 250, 451, 688
VpaK11BI GGWCC 2 cut(s) 117, 299
XapI RAATTY 3 cut(s) 52, 202, 749
XceI RCATGY 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 185
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.