RLG00000033499

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0030948
Physical Location & Seq
Forward (+)
28105828 .. 28110063
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033499

Sequence Viewer

Length: 2277 bp
ATGGCTTTTCAACGCATTAACCTTGTGTTTATTTCCATATTCATTACCTTGGGGACTTTGGCTTCTCATGCCACATCCCCTTCGTTGTATGAAGCTTCCCTTGCTGAAAAACATGAGCAATGGATGGCAAAGCACGGACGCGTTTACCCCAATGCGGCAGAGAAGGAAATGCGTTTCGGCATCTTCAGGAAGAATGTCGAATACGTGGAGAAGTTCAGCAATGAAGCGAATAAGACCTACAAGTTGAACATCAACAGGTTTTCTGATATGACCAACGAGGAATTCCGGAGACATCACTCCGGATTCAAAATGCCCCCCGGCTTAAGTTCAACGTCCTCCAATTTTAGCTACCAAAGCTTGGCTGCTACAGATATTCCAACTAGCGTGGACTGGAGGGAACAACAAGCTGTCACCCCCATAAAAGATCAAAATTCATGCGATGCTAGCTGGGCATTTACAGTAGCAGCAGCTGTGGAAGGGCTAACCAAAATCAAAACCGGCCAATTGATCTCACTCTCCGAGCAGCAACTTGTGGACTGCAATCATCAGAGCTCTGGGTGCAACGGTAGTAGCTTAGAATCCGCCTATTCCTACGTAGTACAAAACGGAGGACTCGCTCGTGAAGAAACTTACCCCTACCAGGCAACAGACATGGGAACATGCGATACTAACAAGGAAAGTGAGCATGCGGCACAGGTCACTGGCTATGAACGCGTCCCTTCCAGAAGTGAAAACGATCTACAGAAGGCTGTGGCCATGCAACCAGTATCGGTTGGCATTACTGCTAAGGGACAAGATTTTCAGATGTACGGCAGTGGGGTGTTCTCCGGCAATTGTGGGACGGGGATCGACCACGCCGTCACGGCCATTGGCTACGGGACGACTGAAGATGGAACCCCCTATTGGTTAATGAAGAACTCATGGGGGGAGGGTTGGGGTGAAAGTGGGTATATGAGGATTCTTAGAAACATTGATGCCCCAGAGGGTATGTGCGGCCTTGCTCTGAATTCCGTCTATCCGACTGGTCCAGTACTTCCACCTAAGCCGACTGCTCCAGCACTTCCACCTAAGCCGACTGCTCCAGCACTTCCACCTAAGCCAACTGCTCCAGTACTTCCACCTAAGCAAGGAAAGAAGAGTAACACAAAAAGAAATATTATCGTCATAATTGTGGTTGTGATTGCTTTTGTCACGATGCTTCTCGGTGGCATATACATTTTCTTTAGAGTCAAGAAGCCAAGGGTAAAACTTGACGACCCAACCAATCATGAATATTTGGATTGGGAAACACGTTACAAAATCATAGAAGGAATTGTTCGTGGTCTTCTTTACCTCCATGAAGATTCTCGACTTCGAATTATTCACCGTGATCTCAAAGCCAGCAACATTTTATTGGATGAAGATATGAAACCTAAAATTGCAGATTTTGGTATGGCAAGGTTGTTTGTGATGGATCAAACTCAAGGAGATACAAAAACCATTGTTGGCACCTATGGATACATGGCACCTGAATATGCAAATCATGGGCGCTTTTCTGTCAAGTCAGATGTCTTTAGTTTTGGTGTGTTAGTTCTTGAAATAATTACTGGCAAGAAGAGTGGTAGTTTCCGAAATGGTGAAAATGAAGACGACCTATTAAGCTATGCCTGGAGAAATTGGAGGGATGATACAACTCTAAATATCATAGATCCCATCTTAACAACAGGCTCAAGATCTGAAATAATGAGATGCATCCGGATTGGATTATTATGTGTCCAAGAAAATTTGAATGATAGACCAACCATAGGGATATTTGGTGGTCAACAACACCACTCTATATTGGCCAACATCTGCAAGTCGCCGGTGCCGGAAAAGAGAAGGCACCCTAGCACTCCAGCTCGAAATGGACCTCCAGATCCAAAGTTTCCAAATTCCAAGGGTCTAGAAACCAGAAGCCCAATATTGGGCCCAAAGTTTTGCAAAACTCAAAAACAAGGAACTCCAATTTACTATGTACACCCTAAGAAGAGATGTCTGCAACCTTTCACTAGAGGTCGGACACCCTTCTGCATCTCCTCCGTCATAGATTCCGGCCAGCCCCACAGCCATCGTCCAACCCACTCTGGCCAAATTGTAGGCACTCTTACCGTCACCCTCACCACTGAAGTTATCAAAAACTTGGCATCGATTGGAGTCCGAGTACAACCCAACCACCCCACTGACCTGGGCACCCAGACATCCGTTGTTCTTTCCATCTCTGGCCACGATTGGAACCACTCCGACCAAAGCACATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

759

Amino Acids

83.92

Weight (kDa)

8.76

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 38 - 95 3.3e-17 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 340 3.3e-74 Papain family cysteine protease
PK_Tyr_Ser-Thr PF07714 423 - 533 8.1e-25 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 424 - 539 3.8e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030948)

Species Orthologous Gene IDs
rosa_laevigata RLG00000033499
rosa_multiflora Rmu_sc0004596.1_g000015

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
AccB1I GGYRCC 5 cut(s) 1487, 1504, 1843, 1860, 2207
AccB7I CCANNNNNTGG 1 cut(s) 358
AccII CGCG 2 cut(s) 141, 714
AccIII TCCGGA 3 cut(s) 285, 299, 1734
AciI CCGC 4 cut(s) 155, 582, 689, 993
AclWI GGATC 4 cut(s) 854, 1461, 1682, 1889
AcoI YGGCCR 7 cut(s) 499, 753, 864, 1821, 2071, 2104, 2239
AcsI RAATTY 5 cut(s) 281, 430, 1006, 1762, 1909
AcuI CTGAAG 3 cut(s) 169, 906, 2163
AfaI GTAC 6 cut(s) 600, 809, 1032, 1113, 1995, 2181
AfiI CCNNNNNNNGG 8 cut(s) 154, 358, 640, 903, 1127, 1784, 1942, 1943
AflII CTTAAG 1 cut(s) 322
AflIII ACRYGT 3 cut(s) 139, 712, 1289
AgsI TTSAA 6 cut(s) 11, 247, 307, 330, 1577, 1768
AjnI CCWGG 3 cut(s) 639, 1646, 2202
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 886, 918
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 4 cut(s) 854, 1461, 1682, 1889
AlwNI CAGNNNCTG 1 cut(s) 470
Aor13HI TCCGGA 3 cut(s) 285, 299, 1734
AoxI GGCC 9 cut(s) 499, 753, 864, 994, 1821, 1945, 2071, 2104, 2239
ApaI GGGCCC 1 cut(s) 1949
ApeKI GCWGC 4 cut(s) 362, 464, 467, 523
ApoI RAATTY 5 cut(s) 281, 430, 1006, 1762, 1909
AspLEI GCGC 1 cut(s) 1530
AspS9I GGNCC 4 cut(s) 1025, 1886, 1945, 1946
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 6 cut(s) 403, 950, 1355, 1628, 2122, 2128
AsuII TTCGAA 1 cut(s) 1354
AsuNHI GCTAGC 1 cut(s) 443
AvaII GGWCC 2 cut(s) 1025, 1886
BaeGI GKGCMC 2 cut(s) 1949, 2210
BalI TGGCCA 4 cut(s) 755, 1823, 2106, 2241
BanI GGYRCC 5 cut(s) 1487, 1504, 1843, 1860, 2207
BanII GRGCYC 2 cut(s) 554, 1949
BauI CACGAG 1 cut(s) 618
BbsI GAAGAC 2 cut(s) 1316, 1632
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 349, 476, 479, 535
BccI CCATC 6 cut(s) 118, 884, 1444, 1700, 2094, 2240
BceAI ACGGC 3 cut(s) 826, 842, 879
BciT130I CCWGG 3 cut(s) 641, 1648, 2204
BciVI GTATCC 1 cut(s) 1490
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 5 cut(s) 381, 444, 1866, 1922, 2028
BfmI CTRYAG 2 cut(s) 366, 740
BfoI RGCGCY 1 cut(s) 1531
BfrI CTTAAG 1 cut(s) 322
BfuI GTATCC 1 cut(s) 1490
BglI GCCNNNNNGGC 1 cut(s) 863
BglII AGATCT 1 cut(s) 1712
BisI GCNGC 7 cut(s) 156, 363, 465, 468, 524, 690, 994
BlsI GCNGC 7 cut(s) 157, 364, 466, 469, 525, 691, 995
BmcAI AGTACT 2 cut(s) 1032, 1113
Bme1390I CCNGG 4 cut(s) 318, 641, 1648, 2204
Bme18I GGWCC 2 cut(s) 1025, 1886
BmgT120I GGNCC 4 cut(s) 1025, 1886, 1945, 1946
BmiI GGNNCC 8 cut(s) 895, 1489, 1506, 1845, 1862, 1947, 2209, 2252
BmrFI CCNGG 4 cut(s) 318, 641, 1648, 2204
BmsI GCATC 8 cut(s) 189, 430, 964, 1185, 1718, 1740, 2058, 2171
BmtI GCTAGC 1 cut(s) 447
BpiI GAAGAC 2 cut(s) 1316, 1632
BpmI CTGGAG 7 cut(s) 412, 1038, 1065, 1092, 1669, 1857, 1875
Bpu10I CCTNAGC 5 cut(s) 786, 1041, 1068, 1095, 1122
Bpu14I TTCGAA 1 cut(s) 1354
BpuEI CTTGAG 2 cut(s) 1446, 1693
BpuMI CCSGG 1 cut(s) 318
Bsa29I ATCGAT 1 cut(s) 2165
BsaAI YACGTR 2 cut(s) 205, 595
BsaJI CCNNGG 5 cut(s) 48, 316, 1238, 1914, 2203
BsaWI WCCGGW 3 cut(s) 285, 299, 1734
BsaXI ACNNNNNCTCC 2 cut(s) 2163, 2193
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 358, 640, 903, 1127, 1784, 1942, 1943
Bse118I RCCGGY 2 cut(s) 497, 1840
Bse1I ACTGG 7 cut(s) 395, 706, 764, 1027, 1028, 1109, 1591
Bse3DI GCAATG 2 cut(s) 125, 226
BseAI TCCGGA 3 cut(s) 285, 299, 1734
BseBI CCWGG 3 cut(s) 641, 1648, 2204
BseCI ATCGAT 1 cut(s) 2165
BseDI CCNNGG 5 cut(s) 48, 316, 1238, 1914, 2203
BseGI GGATG 7 cut(s) 74, 129, 1402, 1669, 1731, 2216, 2270
BseLI CCNNNNNNNGG 8 cut(s) 154, 358, 640, 903, 1127, 1784, 1942, 1943
BseMI GCAATG 2 cut(s) 125, 226
BseNI ACTGG 7 cut(s) 395, 706, 764, 1027, 1028, 1109, 1591
BseRI GAGGAG 1 cut(s) 2044
BseSI GKGCMC 2 cut(s) 1949, 2210
BseXI GCAGC 4 cut(s) 349, 476, 479, 535
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 2 cut(s) 141, 714
BshFI GGCC 9 cut(s) 501, 755, 866, 996, 1823, 1947, 2073, 2106, 2241
BshNI GGYRCC 5 cut(s) 1487, 1504, 1843, 1860, 2207
BshVI ATCGAT 1 cut(s) 2165
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 9 cut(s) 286, 300, 318, 498, 828, 1735, 1841, 1847, 2070
BslFI GGGAC 5 cut(s) 67, 701, 804, 853, 892
BslI CCNNNNNNNGG 8 cut(s) 154, 358, 640, 903, 1127, 1784, 1942, 1943
BsmAI GTCTC 1 cut(s) 283
BsmFI GGGAC 5 cut(s) 67, 701, 804, 853, 892
BsnI GGCC 9 cut(s) 501, 755, 866, 996, 1823, 1947, 2073, 2106, 2241
Bsp119I TTCGAA 1 cut(s) 1354
Bsp120I GGGCCC 1 cut(s) 1945
Bsp1286I GDGCHC 3 cut(s) 554, 1949, 2210
Bsp13I TCCGGA 3 cut(s) 285, 299, 1734
Bsp1407I TGTACA 1 cut(s) 1993
Bsp143I GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
BspACI CCGC 4 cut(s) 155, 582, 689, 993
BspANI GGCC 9 cut(s) 501, 755, 866, 996, 1823, 1947, 2073, 2106, 2241
BspDI ATCGAT 1 cut(s) 2165
BspEI TCCGGA 3 cut(s) 285, 299, 1734
BspFNI CGCG 2 cut(s) 141, 714
BspHI TCATGA 1 cut(s) 1267
BspLI GGNNCC 8 cut(s) 895, 1489, 1506, 1845, 1862, 1947, 2209, 2252
BspOI GCTAGC 1 cut(s) 447
BspPI GGATC 4 cut(s) 854, 1461, 1682, 1889
BspT104I TTCGAA 1 cut(s) 1354
BspT107I GGYRCC 5 cut(s) 1487, 1504, 1843, 1860, 2207
BspTI CTTAAG 1 cut(s) 322
BsrDI GCAATG 2 cut(s) 125, 226
BsrFI RCCGGY 2 cut(s) 497, 1840
BsrGI TGTACA 1 cut(s) 1993
BsrI ACTGG 7 cut(s) 395, 706, 764, 1027, 1028, 1109, 1591
BssAI RCCGGY 2 cut(s) 497, 1840
BssECI CCNNGG 5 cut(s) 48, 316, 1238, 1914, 2203
BssMI GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 3 cut(s) 48, 1238, 1914
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 3 cut(s) 641, 1648, 2204
Bst4CI ACNGT 4 cut(s) 460, 566, 1367, 2128
Bst6I CTCTTC 3 cut(s) 1130, 1589, 2000
BstAFI CTTAAG 1 cut(s) 322
BstAUI TGTACA 1 cut(s) 1993
BstBAI YACGTR 2 cut(s) 205, 595
BstBI TTCGAA 1 cut(s) 1354
BstC8I GCNNGC 4 cut(s) 445, 687, 1381, 2075
BstDEI CTNAG 8 cut(s) 574, 786, 962, 1041, 1068, 1095, 1122, 2001
BstENI CCTNNNNNAGG 1 cut(s) 1125
BstF5I GGATG 7 cut(s) 74, 129, 1402, 1669, 1731, 2216, 2270
BstFNI CGCG 2 cut(s) 141, 714
BstH2I RGCGCY 1 cut(s) 1531
BstHHI GCGC 1 cut(s) 1530
BstKTI GATC 9 cut(s) 427, 510, 739, 849, 1372, 1456, 1690, 1715, 1897
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
BstMWI GCNNNNNNNGC 8 cut(s) 68, 101, 354, 444, 449, 558, 711, 863
BstNI CCWGG 3 cut(s) 641, 1648, 2204
BstNSI RCATGY 2 cut(s) 663, 689
BstSCI CCNGG 4 cut(s) 316, 639, 1646, 2202
BstSFI CTRYAG 2 cut(s) 366, 740
BstSLI GKGCMC 2 cut(s) 1949, 2210
BstSNI TACGTA 1 cut(s) 595
BstUI CGCG 2 cut(s) 141, 714
BstV1I GCAGC 4 cut(s) 349, 476, 479, 535
BstV2I GAAGAC 2 cut(s) 1316, 1632
BstX2I RGATCY 3 cut(s) 1687, 1712, 1894
BstXI CCANNNNNNTGG 1 cut(s) 2203
BstYI RGATCY 3 cut(s) 1687, 1712, 1894
Bsu15I ATCGAT 1 cut(s) 2165
BsuI GTATCC 1 cut(s) 1490
BsuRI GGCC 9 cut(s) 501, 755, 866, 996, 1823, 1947, 2073, 2106, 2241
BsuTUI ATCGAT 1 cut(s) 2165
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 7 cut(s) 74, 129, 1402, 1669, 1731, 2216, 2270
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 4 cut(s) 699, 820, 2139, 2196
Cac8I GCNNGC 4 cut(s) 445, 687, 1381, 2075
CaiI CAGNNNCTG 1 cut(s) 470
CciI TCATGA 1 cut(s) 1267
CfoI GCGC 1 cut(s) 1530
Cfr10I RCCGGY 2 cut(s) 497, 1840
Cfr13I GGNCC 4 cut(s) 1025, 1886, 1945, 1946
ClaI ATCGAT 1 cut(s) 2165
CseI GACGC 2 cut(s) 147, 703
Csp6I GTAC 6 cut(s) 599, 808, 1031, 1112, 1994, 2180
CspCI CAANNNNNGTGG 4 cut(s) 366, 401, 1579, 1614
CviQI GTAC 6 cut(s) 599, 808, 1031, 1112, 1994, 2180
DdeI CTNAG 8 cut(s) 574, 786, 962, 1041, 1068, 1095, 1122, 2001
DpnI GATC 9 cut(s) 426, 509, 738, 848, 1371, 1455, 1689, 1714, 1896
DpnII GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 7 cut(s) 499, 753, 864, 1821, 2071, 2104, 2239
Eam1104I CTCTTC 3 cut(s) 1130, 1589, 2000
EarI CTCTTC 3 cut(s) 1130, 1589, 2000
EciI GGCGGA 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 552
Eco105I TACGTA 1 cut(s) 595
Eco130I CCWWGG 3 cut(s) 48, 1238, 1914
Eco24I GRGCYC 2 cut(s) 554, 1949
Eco47I GGWCC 2 cut(s) 1025, 1886
Eco53kI GAGCTC 1 cut(s) 552
Eco57I CTGAAG 3 cut(s) 169, 906, 2163
EcoICRI GAGCTC 1 cut(s) 552
EcoNI CCTNNNNNAGG 1 cut(s) 1125
EcoRI GAATTC 2 cut(s) 281, 1006
EcoRII CCWGG 3 cut(s) 639, 1646, 2202
EcoT14I CCWWGG 3 cut(s) 48, 1238, 1914
EcoT22I ATGCAT 1 cut(s) 1733
EcoT38I GRGCYC 2 cut(s) 554, 1949
ErhI CCWWGG 3 cut(s) 48, 1238, 1914
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 5 cut(s) 67, 701, 804, 853, 892
Fnu4HI GCNGC 7 cut(s) 156, 363, 465, 468, 524, 690, 994
FokI GGATG 7 cut(s) 61, 136, 1409, 1676, 1718, 2203, 2257
FriOI GRGCYC 2 cut(s) 554, 1949
Fsp4HI GCNGC 7 cut(s) 156, 363, 465, 468, 524, 690, 994
FspBI CTAG 5 cut(s) 381, 444, 1866, 1922, 2028
GlaI GCGC 1 cut(s) 1529
GluI GCNGC 7 cut(s) 156, 363, 465, 468, 524, 690, 994
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 7 cut(s) 412, 1038, 1065, 1092, 1669, 1857, 1875
HaeII RGCGCY 1 cut(s) 1531
HaeIII GGCC 9 cut(s) 501, 755, 866, 996, 1823, 1947, 2073, 2106, 2241
HapII CCGG 9 cut(s) 286, 300, 318, 498, 828, 1735, 1841, 1847, 2070
HgaI GACGC 2 cut(s) 147, 703
HhaI GCGC 1 cut(s) 1530
Hin6I GCGC 1 cut(s) 1528
HinP1I GCGC 1 cut(s) 1528
HincII GTYRAC 1 cut(s) 1802
HindII GTYRAC 1 cut(s) 1802
HindIII AAGCTT 2 cut(s) 93, 355
HinfI GANTC 8 cut(s) 303, 578, 612, 958, 1227, 1343, 2066, 2172
HpaII CCGG 9 cut(s) 286, 300, 318, 498, 828, 1735, 1841, 1847, 2070
HphI GGTGA 6 cut(s) 403, 950, 1355, 1628, 2122, 2128
Hpy166II GTNNAC 5 cut(s) 145, 388, 535, 1802, 1996
Hpy8I GTNNAC 5 cut(s) 145, 388, 535, 1802, 1996
HpyAV CCTTC 8 cut(s) 90, 157, 470, 729, 739, 1301, 1851, 2053
HpyCH4III ACNGT 4 cut(s) 460, 566, 1367, 2128
HpyCH4IV ACGT 4 cut(s) 204, 332, 594, 1291
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 101, 354, 444, 449, 558, 711, 863
HpyF3I CTNAG 8 cut(s) 574, 786, 962, 1041, 1068, 1095, 1122, 2001
HpySE526I ACGT 4 cut(s) 204, 332, 594, 1291
HspAI GCGC 1 cut(s) 1528
Kpn2I TCCGGA 3 cut(s) 285, 299, 1734
Kzo9I GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
LmnI GCTCC 3 cut(s) 1057, 1084, 1111
Lsp1109I GCAGC 4 cut(s) 349, 476, 479, 535
LweI GCATC 8 cut(s) 189, 430, 964, 1185, 1718, 1740, 2058, 2171
MaeI CTAG 5 cut(s) 381, 444, 1866, 1922, 2028
MaeII ACGT 4 cut(s) 204, 332, 594, 1291
MaeIII GTNAC 7 cut(s) 409, 697, 859, 1139, 1189, 1292, 2128
MalI GATC 9 cut(s) 426, 509, 738, 848, 1371, 1455, 1689, 1714, 1896
MboI GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
MfeI CAATTG 2 cut(s) 503, 832
MflI RGATCY 3 cut(s) 1687, 1712, 1894
MhlI GDGCHC 3 cut(s) 554, 1949, 2210
MlsI TGGCCA 4 cut(s) 755, 1823, 2106, 2241
MluI ACGCGT 2 cut(s) 139, 712
MluNI TGGCCA 4 cut(s) 755, 1823, 2106, 2241
MlyI GAGTC 3 cut(s) 606, 1236, 2181
MmeI TCCRAC 4 cut(s) 401, 1043, 2015, 2117
Mox20I TGGCCA 4 cut(s) 755, 1823, 2106, 2241
Mph1103I ATGCAT 1 cut(s) 1733
MroI TCCGGA 3 cut(s) 285, 299, 1734
MscI TGGCCA 4 cut(s) 755, 1823, 2106, 2241
MseI TTAA 5 cut(s) 18, 323, 908, 1637, 1697
MslI CAYNNNNRTG 1 cut(s) 1169
Msp20I TGGCCA 4 cut(s) 755, 1823, 2106, 2241
MspA1I CMGCKG 1 cut(s) 470
MspCI CTTAAG 1 cut(s) 322
MspI CCGG 9 cut(s) 286, 300, 318, 498, 828, 1735, 1841, 1847, 2070
MspR9I CCNGG 4 cut(s) 318, 641, 1648, 2204
MunI CAATTG 2 cut(s) 503, 832
MvaI CCWGG 3 cut(s) 641, 1648, 2204
MvnI CGCG 2 cut(s) 141, 714
MwoI GCNNNNNNNGC 8 cut(s) 68, 101, 354, 444, 449, 558, 711, 863
NciI CCSGG 1 cut(s) 318
NdeII GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
NheI GCTAGC 1 cut(s) 443
NlaIV GGNNCC 8 cut(s) 895, 1489, 1506, 1845, 1862, 1947, 2209, 2252
NmuCI GTSAC 5 cut(s) 409, 697, 859, 1189, 2128
NsiI ATGCAT 1 cut(s) 1733
NspI RCATGY 2 cut(s) 663, 689
NspV TTCGAA 1 cut(s) 1354
PaeI GCATGC 1 cut(s) 689
PagI TCATGA 1 cut(s) 1267
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PfeI GAWTC 5 cut(s) 303, 578, 958, 1343, 2066
PflMI CCANNNNNTGG 1 cut(s) 358
PkrI GCNGC 7 cut(s) 157, 364, 466, 469, 525, 691, 995
PleI GAGTC 3 cut(s) 606, 1235, 2180
PpsI GAGTC 3 cut(s) 606, 1235, 2180
Ppu21I YACGTR 2 cut(s) 205, 595
Psp124BI GAGCTC 1 cut(s) 554
Psp6I CCWGG 3 cut(s) 639, 1646, 2202
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 3 cut(s) 639, 1646, 2202
PspN4I GGNNCC 8 cut(s) 895, 1489, 1506, 1845, 1862, 1947, 2209, 2252
PspOMI GGGCCC 1 cut(s) 1945
PspPI GGNCC 4 cut(s) 1025, 1886, 1945, 1946
PsrI GAACNNNNNNTAC 2 cut(s) 649, 681
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 3 cut(s) 1687, 1712, 1894
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 6 cut(s) 600, 809, 1032, 1113, 1995, 2181
RsaNI GTAC 6 cut(s) 599, 808, 1031, 1112, 1994, 2180
RseI CAYNNNNRTG 1 cut(s) 1169
SacI GAGCTC 1 cut(s) 554
SaqAI TTAA 5 cut(s) 18, 323, 908, 1637, 1697
SatI GCNGC 7 cut(s) 156, 363, 465, 468, 524, 690, 994
Sau3AI GATC 9 cut(s) 424, 507, 736, 846, 1369, 1453, 1687, 1712, 1894
Sau96I GGNCC 4 cut(s) 1025, 1886, 1945, 1946
ScaI AGTACT 2 cut(s) 1032, 1113
SchI GAGTC 3 cut(s) 606, 1236, 2181
ScrFI CCNGG 4 cut(s) 318, 641, 1648, 2204
SduI GDGCHC 3 cut(s) 554, 1949, 2210
SfaNI GCATC 8 cut(s) 189, 430, 964, 1185, 1718, 1740, 2058, 2171
SfcI CTRYAG 2 cut(s) 366, 740
SfuI TTCGAA 1 cut(s) 1354
SgrAI CRCCGGYG 1 cut(s) 1840
SinI GGWCC 2 cut(s) 1025, 1886
SmiMI CAYNNNNRTG 1 cut(s) 1169
SmlI CTYRAG 3 cut(s) 322, 1461, 1708
SmoI CTYRAG 3 cut(s) 322, 1461, 1708
SnaBI TACGTA 1 cut(s) 595
SphI GCATGC 1 cut(s) 689
SsiI CCGC 4 cut(s) 155, 582, 689, 993
SspI AATATT 3 cut(s) 1156, 1274, 1941
SspMI CTAG 5 cut(s) 381, 444, 1866, 1922, 2028
SstI GAGCTC 1 cut(s) 554
StyD4I CCNGG 4 cut(s) 316, 639, 1646, 2202
StyI CCWWGG 3 cut(s) 48, 1238, 1914
TaaI ACNGT 4 cut(s) 460, 566, 1367, 2128
TaiI ACGT 4 cut(s) 207, 335, 597, 1294
TaqI TCGA 6 cut(s) 198, 849, 1348, 1354, 1879, 2165
TatI WGTACW 5 cut(s) 598, 1030, 1111, 1993, 2179
TauI GCSGC 3 cut(s) 158, 692, 996
TfiI GAWTC 5 cut(s) 303, 578, 958, 1343, 2066
Tru1I TTAA 5 cut(s) 18, 323, 908, 1637, 1697
Tru9I TTAA 5 cut(s) 18, 323, 908, 1637, 1697
TscAI CASTG 4 cut(s) 706, 820, 2146, 2203
TseFI GTSAC 5 cut(s) 409, 697, 859, 1189, 2128
TseI GCWGC 4 cut(s) 362, 464, 467, 523
Tsp45I GTSAC 5 cut(s) 409, 697, 859, 1189, 2128
TspGWI ACGGA 5 cut(s) 150, 621, 1000, 2047, 2209
TspRI CASTG 4 cut(s) 706, 820, 2146, 2203
Van91I CCANNNNNTGG 1 cut(s) 358
Vha464I CTTAAG 1 cut(s) 322
VpaK11BI GGWCC 2 cut(s) 1025, 1886
XagI CCTNNNNNAGG 1 cut(s) 1125
XapI RAATTY 5 cut(s) 281, 430, 1006, 1762, 1909
XbaI TCTAGA 1 cut(s) 1921
XceI RCATGY 2 cut(s) 663, 689
XspI CTAG 5 cut(s) 381, 444, 1866, 1922, 2028
ZrmI AGTACT 2 cut(s) 1032, 1113
Zsp2I ATGCAT 1 cut(s) 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.