Rmu_co8250683.1_g000001

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8250683.1
OG0023027
Physical Location & Seq
Forward (+)
70 .. 573
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8250683.1_g000001.1.cds

Sequence Viewer

Length: 504 bp
atggtcactcaatttggaaccggtaccccttcctcttcttctttcacccataaacattcttggacatatgatgtctttctaagtttcagaggtcaggatacaagcagaaattttacagaccatttgtatagcgctttgagtcaacaggggattaagaccttcgataacgttgaggttccaaaaggagaagtgaatggcccggcgattttcagagcaatcgaagagtcaagaatttctgtcatcatattatctcacaattatgcaacttcaaggtggtgtttggaggaattggtgaagatccttggatgtagaaactcaaagcaacaacaggttattcctgtgttctacaaggttagcccctccgatgtaaggacctgtcaggttggttttggtgaggcattagctgcgcatgaaattgagttcaaggataagatatatgaatggagagcagctcttatggaagtagcaaatctctctgggtggcattttccggaggacgggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

167

Amino Acids

18.9

Weight (kDa)

6.05

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023027)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8250683.1_g000001 Rmu_sc0002938.1_g000008 Rmu_ssc0000388.1_g000025
rosa_roxburghii Rroxscaffold_7G00201100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 408
Acc65I GGTACC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 490
AclI AACGTT 1 cut(s) 168
AclWI GGATC 1 cut(s) 292
AcsI RAATTY 2 cut(s) 109, 231
AfaI GTAC 1 cut(s) 25
AfeI AGCGCT 1 cut(s) 133
AfiI CCNNNNNNNGG 2 cut(s) 369, 497
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 2 cut(s) 270, 424
AluBI AGCT 2 cut(s) 404, 452
AluI AGCT 2 cut(s) 404, 452
AlwI GGATC 1 cut(s) 292
Aor13HI TCCGGA 1 cut(s) 490
Aor51HI AGCGCT 1 cut(s) 133
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 2 cut(s) 404, 449
ApoI RAATTY 2 cut(s) 109, 231
AsiGI ACCGGT 1 cut(s) 20
Asp718I GGTACC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 134, 409
AspS9I GGNCC 2 cut(s) 197, 372
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 37, 304, 404
AvaII GGWCC 1 cut(s) 372
BanI GGYRCC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 391, 461
BciVI GTATCC 1 cut(s) 91
BcnI CCSGG 1 cut(s) 200
BfoI RGCGCY 1 cut(s) 135
BfuI GTATCC 1 cut(s) 91
BisI GCNGC 2 cut(s) 405, 450
BlsI GCNGC 2 cut(s) 406, 451
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 372
BmgT120I GGNCC 2 cut(s) 197, 372
BmiI GGNNCC 3 cut(s) 19, 25, 177
BmrFI CCNGG 1 cut(s) 200
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpuMI CCSGG 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 301
BsaWI WCCGGW 2 cut(s) 20, 490
BsaXI ACNNNNNCTCC 1 cut(s) 485
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 497
Bse118I RCCGGY 1 cut(s) 20
BseAI TCCGGA 1 cut(s) 490
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 369, 497
BseXI GCAGC 2 cut(s) 391, 461
BshFI GGCC 1 cut(s) 198
BshNI GGYRCC 1 cut(s) 23
BshTI ACCGGT 1 cut(s) 20
BsiSI CCGG 3 cut(s) 21, 200, 491
BslI CCNNNNNNNGG 2 cut(s) 369, 497
BsnI GGCC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 490
Bsp143I GATC 1 cut(s) 297
BspANI GGCC 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 490
BspLI GGNNCC 3 cut(s) 19, 25, 177
BspPI GGATC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 301
Bst6I CTCTTC 2 cut(s) 40, 216
BstAPI GCANNNNNTGC 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 2 cut(s) 134, 409
BstKTI GATC 1 cut(s) 300
BstMBI GATC 1 cut(s) 297
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 391, 461
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BsuI GTATCC 1 cut(s) 91
BsuRI GGCC 1 cut(s) 198
BtsCI GGATG 1 cut(s) 311
CfoI GCGC 2 cut(s) 134, 409
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 197, 372
Csp6I GTAC 1 cut(s) 24
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 1 cut(s) 410
CviJI RGCY 4 cut(s) 198, 357, 404, 452
CviKI_1 RGCY 4 cut(s) 198, 357, 404, 452
CviQI GTAC 1 cut(s) 24
DdeI CTNAG 1 cut(s) 80
DpnI GATC 1 cut(s) 299
DpnII GATC 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 40, 216
EarI CTCTTC 2 cut(s) 40, 216
Eco130I CCWWGG 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 372
Eco47III AGCGCT 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 1 cut(s) 413
FatI CATG 1 cut(s) 409
FauNDI CATATG 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 405, 450
FokI GGATG 1 cut(s) 318
Fsp4HI GCNGC 2 cut(s) 405, 450
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 2 cut(s) 133, 408
GluI GCNGC 2 cut(s) 405, 450
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 198
HapII CCGG 3 cut(s) 21, 200, 491
HhaI GCGC 2 cut(s) 134, 409
Hin1II CATG 1 cut(s) 413
Hin6I GCGC 2 cut(s) 132, 407
HinP1I GCGC 2 cut(s) 132, 407
HincII GTYRAC 1 cut(s) 143
HindII GTYRAC 1 cut(s) 143
HinfI GANTC 2 cut(s) 139, 224
HpaII CCGG 3 cut(s) 21, 200, 491
HphI GGTGA 3 cut(s) 37, 304, 404
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 3 cut(s) 89, 212, 364
Hpy188III TCNNGA 3 cut(s) 95, 228, 491
Hpy8I GTNNAC 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 39, 169
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 1 cut(s) 413
HspAI GCGC 2 cut(s) 132, 407
Kpn2I TCCGGA 1 cut(s) 490
KpnI GGTACC 1 cut(s) 27
Kzo9I GATC 1 cut(s) 297
LpnPI CCDG 9 cut(s) 34, 80, 131, 213, 314, 351, 365, 388, 462
Lsp1109I GCAGC 2 cut(s) 391, 461
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 4
MalI GATC 1 cut(s) 299
MboI GATC 1 cut(s) 297
MboII GAAGA 4 cut(s) 27, 30, 233, 307
MflI RGATCY 1 cut(s) 297
MluCI AATT 6 cut(s) 11, 109, 231, 256, 287, 414
MlyI GAGTC 2 cut(s) 148, 233
MnlI CCTC 7 cut(s) 43, 83, 166, 277, 370, 388, 487
MroI TCCGGA 1 cut(s) 490
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 258
MspI CCGG 3 cut(s) 21, 200, 491
MspR9I CCNGG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 404
NciI CCSGG 1 cut(s) 200
NdeI CATATG 1 cut(s) 67
NdeII GATC 1 cut(s) 297
NlaIII CATG 1 cut(s) 413
NlaIV GGNNCC 3 cut(s) 19, 25, 177
NmuCI GTSAC 1 cut(s) 4
NsbI TGCGCA 1 cut(s) 408
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 2 cut(s) 406, 451
PleI GAGTC 2 cut(s) 147, 232
PpsI GAGTC 2 cut(s) 147, 232
PpuMI RGGWCCY 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 372
PspN4I GGNNCC 3 cut(s) 19, 25, 177
PspPI GGNCC 2 cut(s) 197, 372
PspPPI RGGWCCY 1 cut(s) 372
PsuI RGATCY 1 cut(s) 297
RsaI GTAC 1 cut(s) 25
RsaNI GTAC 1 cut(s) 24
RseI CAYNNNNRTG 1 cut(s) 258
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 2 cut(s) 405, 450
Sau3AI GATC 1 cut(s) 297
Sau96I GGNCC 2 cut(s) 197, 372
SchI GAGTC 2 cut(s) 148, 233
ScrFI CCNGG 1 cut(s) 200
SinI GGWCC 1 cut(s) 372
SmiMI CAYNNNNRTG 1 cut(s) 258
Sse9I AATT 6 cut(s) 11, 109, 231, 256, 287, 414
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 301
TaiI ACGT 1 cut(s) 171
TaqI TCGA 2 cut(s) 162, 219
TasI AATT 6 cut(s) 11, 109, 231, 256, 287, 414
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 2 cut(s) 404, 449
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 426, 453
VpaK11BI GGWCC 1 cut(s) 372
XapI RAATTY 2 cut(s) 109, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.