Rmu_co8254497.1_g000001

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8254497.1
Physical Location & Seq
Reverse (-)
1 .. 640
640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8254497.1_g000001.1.cds

Sequence Viewer

Length: 445 bp
atgaatgttgtaaataagaagaacaaaaatgccaacctcaaggctcacaacgtgaagaattatctttgggtctttgtcaatgctcttattgacaaccctgcctttgattcccaaacaaaggaaactttgacgattcgcccaagcagttttgggtctaaatgtgaactaccgcaagagttcttaaagaaagttgcaaagtctggggttgtggaaagtcttctctcttgggcaagttttaagcagaacaaagatctgaagaaaactgatggaacaaaaacggagaggcttcataacatcaacaagctggaggatgctaacttagcaggaggaaggaactctgaaaagtgtaccttgattttaacagagggagattcagctaaggctcttgcaatggcgggactggctgttgttggtcgggaccattatggtgtttttccattgagag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling

Protein Analysis

148

Amino Acids

16.36

Weight (kDa)

9.57

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready

Orthologous Genes (Group: OG0029228)

Species Orthologous Gene IDs
pyrus_communis pycom02g25930
rosa_multiflora Rmu_co8254497.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 170, 395
AcuI CTGAAG 1 cut(s) 275
AdeI CACNNNGTG 1 cut(s) 52
AfaI GTAC 1 cut(s) 349
AfiI CCNNNNNNNGG 1 cut(s) 118
AluBI AGCT 2 cut(s) 304, 377
AluI AGCT 2 cut(s) 304, 377
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 418
AvaII GGWCC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 209
BccI CCATC 1 cut(s) 260
BglII AGATCT 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 1 cut(s) 419
BmsI GCATC 1 cut(s) 301
BpiI GAAGAC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 378
BpuEI CTTGAG 1 cut(s) 23
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 396
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 396
BseNI ACTGG 1 cut(s) 405
BslFI GGGAC 2 cut(s) 411, 431
BslI CCNNNNNNNGG 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 411, 431
Bsp143I GATC 1 cut(s) 250
BspACI CCGC 2 cut(s) 170, 395
BspLI GGNNCC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 1 cut(s) 405
BssMI GATC 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 319, 378
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 1 cut(s) 253
BstMBI GATC 1 cut(s) 250
BstMWI GCNNNNNNNGC 2 cut(s) 320, 401
BstV2I GAAGAC 1 cut(s) 209
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BtsCI GGATG 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 418
Csp6I GTAC 1 cut(s) 348
CviJI RGCY 6 cut(s) 44, 286, 304, 377, 383, 404
CviKI_1 RGCY 6 cut(s) 44, 286, 304, 377, 383, 404
CviQI GTAC 1 cut(s) 348
DdeI CTNAG 2 cut(s) 319, 378
DpnI GATC 1 cut(s) 252
DpnII GATC 1 cut(s) 250
DraIII CACNNNGTG 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 275
FaiI YATR 2 cut(s) 291, 426
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 2 cut(s) 411, 431
FauI CCCGC 1 cut(s) 388
FokI GGATG 1 cut(s) 323
GsuI CTGGAG 1 cut(s) 326
HinfI GANTC 3 cut(s) 107, 133, 371
Hpy166II GTNNAC 2 cut(s) 164, 348
Hpy188I TCNGA 2 cut(s) 255, 340
Hpy188III TCNNGA 1 cut(s) 416
Hpy8I GTNNAC 2 cut(s) 164, 348
HpyAV CCTTC 1 cut(s) 324
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 194, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 401
HpyF3I CTNAG 2 cut(s) 319, 378
HpySE526I ACGT 1 cut(s) 51
Kzo9I GATC 1 cut(s) 250
LpnPI CCDG 5 cut(s) 111, 186, 290, 309, 386
LweI GCATC 1 cut(s) 301
MaeII ACGT 1 cut(s) 51
MalI GATC 1 cut(s) 252
MboI GATC 1 cut(s) 250
MboII GAAGA 4 cut(s) 31, 67, 209, 268
MflI RGATCY 1 cut(s) 250
MluCI AATT 1 cut(s) 58
MnlI CCTC 5 cut(s) 47, 276, 301, 320, 358
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 3 cut(s) 182, 237, 359
MslI CAYNNNNRTG 1 cut(s) 426
MwoI GCNNNNNNNGC 2 cut(s) 320, 401
NdeII GATC 1 cut(s) 250
NlaIV GGNNCC 1 cut(s) 419
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 3 cut(s) 107, 133, 371
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 1 cut(s) 418
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 1 cut(s) 349
RsaNI GTAC 1 cut(s) 348
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 3 cut(s) 182, 237, 359
Sau3AI GATC 1 cut(s) 250
Sau96I GGNCC 1 cut(s) 418
SetI ASST 5 cut(s) 39, 54, 306, 353, 379
SfaNI GCATC 1 cut(s) 301
SinI GGWCC 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 1 cut(s) 58
SsiI CCGC 2 cut(s) 170, 395
TaiI ACGT 1 cut(s) 54
TasI AATT 1 cut(s) 58
TfiI GAWTC 3 cut(s) 107, 133, 371
Tru1I TTAA 3 cut(s) 182, 237, 359
Tru9I TTAA 3 cut(s) 182, 237, 359
TspDTI ATGAA 2 cut(s) 17, 278
TspGWI ACGGA 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 418
XmnI GAANNNNTTC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.