Rmu_sc0000010.1_g000026

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000010.1
OG0031029
Physical Location & Seq
Forward (+)
103871 .. 105584
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1122 bp
atgcagagggctaggttgcccattgctgaagaggacgaggatggagaagaagaagaggaccatgaggaagaaagaggccttgcggggattcttccactgcccgacactcccggcgggccatggaaggtctacggcgtggcccggcggccccggccgcagtggaacgctgaccacccgattcagtacatccagtgtgacctctctgactctacacagaccgaggcagagctctcccagctcactgatgtcactcacattttttatgtcacgtgggcgagcaagccaactgaagctgagaattgtgaggctaatggcaagatgttgagcaatgtgttgaatgctgtgatcccaaacgcgccgaatttgcagcacatatgtatgcagacgggtcggaaacactacttaggttcgtttgagatgttggggaatgtccaaccccatgaacctccatgtcacgaagacctgccgaggcttaatgcccccaatttttactatgtgatggaggatattctgtttgaggaggtgaagaagaaggaggggcttacatggtctgtgcataggccgggactcatattcggcttctcaccttatagtttgatgaatgcgattgggactttgtgtgtttatgctgcgatttgtaagcatgaagggcagccgctcaggtttccggggagtaaaggaacttgggaaggattctgggacgcgtctgatgcggatatggttgctgaacatgaaatatgggcagctgtggagccttatgccaagaatgaagcttttaattgcatcaatggggatgtgttcaagtggaagcatttgtgggggattctggctgagcagttcgagctagtgcctgctggagttcacgaggagttgagttttgaggagatgatgaaagataaagggccagtgtgggatgaaattgtgagagagcatggtttggtgcctaccaaattggaagaggtagggaactggtggtttctggacattatgtttcgacggattgattcaactgcggacagtatgaacaagagcaaagagcatggttttgttggctttaggaacccgaaaactccctttgtttcttggattgacaagatgaagtcttttagaattgttccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

373

Amino Acids

42.63

Weight (kDa)

4.97

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 71 - 320 5e-24 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0031029)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000010.1_g000026 Rmu_sc0003711.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 471
AccB1I GGYRCC 1 cut(s) 940
AccBSI CCGCTC 1 cut(s) 658
AccI GTMKAC 1 cut(s) 129
AccII CGCG 2 cut(s) 356, 704
AciI CCGC 7 cut(s) 83, 114, 145, 155, 656, 713, 1013
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 2 cut(s) 48, 309
AcvI CACGTG 1 cut(s) 270
AfaI GTAC 1 cut(s) 185
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 3 cut(s) 337, 802, 1008
AluBI AGCT 6 cut(s) 229, 238, 293, 746, 773, 844
AluI AGCT 6 cut(s) 229, 238, 293, 746, 773, 844
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 1 cut(s) 340
AoxI GGCC 7 cut(s) 76, 116, 138, 146, 152, 560, 902
ApeKI GCWGC 4 cut(s) 367, 629, 652, 743
ApoI RAATTY 1 cut(s) 361
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 5 cut(s) 58, 116, 139, 147, 902
AsuC2I CCSGG 5 cut(s) 111, 142, 151, 564, 669
AsuHPI GGTGA 2 cut(s) 535, 576
AvaII GGWCC 1 cut(s) 58
BanI GGYRCC 1 cut(s) 940
BanII GRGCYC 1 cut(s) 231
BauI CACGAG 1 cut(s) 863
BbrPI CACGTG 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 379, 616, 664, 755
BccI CCATC 2 cut(s) 35, 493
BceAI ACGGC 1 cut(s) 148
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BcnI CCSGG 5 cut(s) 111, 142, 151, 564, 669
BfaI CTAG 2 cut(s) 12, 845
BfuAI ACCTGC 1 cut(s) 471
BisI GCNGC 7 cut(s) 146, 155, 368, 630, 653, 656, 744
BlpI GCTNAGC 1 cut(s) 831
BlsI GCNGC 7 cut(s) 147, 156, 369, 631, 654, 657, 745
Bme1390I CCNGG 5 cut(s) 111, 142, 151, 564, 669
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 5 cut(s) 58, 116, 139, 147, 902
BmiI GGNNCC 4 cut(s) 149, 753, 942, 1061
BmrFI CCNGG 5 cut(s) 111, 142, 151, 564, 669
BmsI GCATC 2 cut(s) 700, 792
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 876
Bpu10I CCTNAGC 1 cut(s) 659
Bpu1102I GCTNAGC 1 cut(s) 831
BpuMI CCSGG 5 cut(s) 111, 142, 151, 564, 669
BsaAI YACGTR 1 cut(s) 270
BsaJI CCNNGG 5 cut(s) 119, 149, 219, 467, 668
Bse1I ACTGG 3 cut(s) 190, 905, 974
Bse3DI GCAATG 2 cut(s) 21, 334
BseDI CCNNGG 5 cut(s) 119, 149, 219, 467, 668
BseGI GGATG 4 cut(s) 46, 186, 799, 919
BseMI GCAATG 2 cut(s) 21, 334
BseMII CTCAG 3 cut(s) 285, 673, 822
BseNI ACTGG 3 cut(s) 190, 905, 974
BseRI GAGGAG 3 cut(s) 533, 881, 896
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 4 cut(s) 379, 616, 664, 755
BseYI CCCAGC 1 cut(s) 234
Bsh1236I CGCG 2 cut(s) 356, 704
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 7 cut(s) 78, 118, 140, 148, 154, 562, 904
BshNI GGYRCC 1 cut(s) 940
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 5 cut(s) 111, 142, 151, 563, 668
BslFI GGGAC 3 cut(s) 579, 625, 713
BsmFI GGGAC 3 cut(s) 579, 625, 713
BsmI GAATGC 2 cut(s) 343, 607
BsnI GGCC 7 cut(s) 78, 118, 140, 148, 154, 562, 904
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 1 cut(s) 345
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 7 cut(s) 83, 114, 145, 155, 656, 713, 1013
BspANI GGCC 7 cut(s) 78, 118, 140, 148, 154, 562, 904
BspCNI CTCAG 3 cut(s) 286, 672, 823
BspFNI CGCG 2 cut(s) 356, 704
BspLI GGNNCC 4 cut(s) 149, 753, 942, 1061
BspMI ACCTGC 1 cut(s) 471
BspPI GGATC 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 940
BsrBI CCGCTC 1 cut(s) 658
BsrDI GCAATG 2 cut(s) 21, 334
BsrI ACTGG 3 cut(s) 190, 905, 974
BssECI CCNNGG 5 cut(s) 119, 149, 219, 467, 668
BssMI GATC 1 cut(s) 345
BssSI CACGAG 1 cut(s) 863
BssT1I CCWWGG 1 cut(s) 119
Bst2BI CACGAG 1 cut(s) 863
Bst4CI ACNGT 1 cut(s) 1019
Bst6I CTCTTC 3 cut(s) 24, 48, 951
BstBAI YACGTR 1 cut(s) 270
BstC8I GCNNGC 4 cut(s) 116, 277, 281, 852
BstDEI CTNAG 4 cut(s) 294, 403, 659, 831
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 4 cut(s) 46, 186, 799, 919
BstFNI CGCG 2 cut(s) 356, 704
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 1 cut(s) 348
BstMBI GATC 1 cut(s) 345
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 7 cut(s) 151, 154, 235, 364, 649, 710, 841
BstSCI CCNGG 5 cut(s) 109, 140, 149, 562, 667
BstUI CGCG 2 cut(s) 356, 704
BstV1I GCAGC 4 cut(s) 379, 616, 664, 755
BstV2I GAAGAC 1 cut(s) 465
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 7 cut(s) 78, 118, 140, 148, 154, 562, 904
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 4 cut(s) 46, 186, 799, 919
BtsI GCAGTG 2 cut(s) 95, 164
BtsIMutI CAGTG 5 cut(s) 95, 164, 197, 240, 912
BveI ACCTGC 1 cut(s) 471
Cac8I GCNNGC 4 cut(s) 116, 277, 281, 852
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 5 cut(s) 58, 116, 139, 147, 902
CseI GACGC 2 cut(s) 693, 710
Csp6I GTAC 1 cut(s) 184
CviAII CATG 9 cut(s) 62, 120, 440, 450, 546, 644, 731, 932, 1040
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 4 cut(s) 294, 403, 659, 831
DpnI GATC 1 cut(s) 347
DpnII GATC 1 cut(s) 345
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Eam1104I CTCTTC 3 cut(s) 24, 48, 951
EarI CTCTTC 3 cut(s) 24, 48, 951
Ecl136II GAGCTC 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 119
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 58
Eco52I CGGCCG 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 48, 309
Eco72I CACGTG 1 cut(s) 270
EcoICRI GAGCTC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 9 cut(s) 65, 123, 443, 453, 549, 647, 734, 935, 1043
FaqI GGGAC 3 cut(s) 579, 625, 713
FatI CATG 9 cut(s) 61, 119, 439, 449, 545, 643, 730, 931, 1039
FauI CCCGC 2 cut(s) 76, 107
FauNDI CATATG 1 cut(s) 374
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 7 cut(s) 146, 155, 368, 630, 653, 656, 744
FokI GGATG 4 cut(s) 53, 173, 806, 926
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 7 cut(s) 146, 155, 368, 630, 653, 656, 744
FspBI CTAG 2 cut(s) 12, 845
GlaI GCGC 1 cut(s) 357
GluI GCNGC 7 cut(s) 146, 155, 368, 630, 653, 656, 744
GsaI CCCAGC 1 cut(s) 238
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 7 cut(s) 78, 118, 140, 148, 154, 562, 904
HapII CCGG 5 cut(s) 111, 142, 151, 563, 668
HgaI GACGC 2 cut(s) 693, 710
HhaI GCGC 1 cut(s) 358
Hin1II CATG 9 cut(s) 65, 123, 443, 453, 549, 647, 734, 935, 1043
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 771
HinfI GANTC 7 cut(s) 88, 178, 206, 567, 693, 823, 1004
HpaII CCGG 5 cut(s) 111, 142, 151, 563, 668
HphI GGTGA 2 cut(s) 535, 576
Hpy166II GTNNAC 2 cut(s) 130, 862
Hpy188I TCNGA 3 cut(s) 205, 393, 709
Hpy188III TCNNGA 3 cut(s) 455, 863, 980
Hpy8I GTNNAC 2 cut(s) 130, 862
Hpy99I CGWCG 1 cut(s) 999
HpyAV CCTTC 4 cut(s) 118, 526, 641, 683
HpyCH4III ACNGT 1 cut(s) 1019
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 5 cut(s) 4, 367, 382, 556, 783
HpyF10VI GCNNNNNNNGC 7 cut(s) 151, 154, 235, 364, 649, 710, 841
HpyF3I CTNAG 4 cut(s) 294, 403, 659, 831
HpySE526I ACGT 1 cut(s) 269
Hsp92II CATG 9 cut(s) 65, 123, 443, 453, 549, 647, 734, 935, 1043
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 1 cut(s) 345
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 4 cut(s) 379, 616, 664, 755
LweI GCATC 2 cut(s) 700, 792
MaeI CTAG 2 cut(s) 12, 845
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 4 cut(s) 194, 247, 265, 452
MalI GATC 1 cut(s) 347
MbiI CCGCTC 1 cut(s) 658
MboI GATC 1 cut(s) 345
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 7 cut(s) 298, 361, 484, 778, 918, 950, 1110
MluI ACGCGT 1 cut(s) 702
MlyI GAGTC 2 cut(s) 200, 561
MmeI TCCRAC 2 cut(s) 371, 457
MseI TTAA 2 cut(s) 474, 777
MslI CAYNNNNRTG 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 746
MspI CCGG 5 cut(s) 111, 142, 151, 563, 668
MspR9I CCNGG 5 cut(s) 111, 142, 151, 564, 669
Mva1269I GAATGC 2 cut(s) 343, 607
MvnI CGCG 2 cut(s) 356, 704
MwoI GCNNNNNNNGC 7 cut(s) 151, 154, 235, 364, 649, 710, 841
NciI CCSGG 5 cut(s) 111, 142, 151, 564, 669
NcoI CCATGG 1 cut(s) 119
NdeI CATATG 1 cut(s) 374
NdeII GATC 1 cut(s) 345
NlaIII CATG 9 cut(s) 65, 123, 443, 453, 549, 647, 734, 935, 1043
NlaIV GGNNCC 4 cut(s) 149, 753, 942, 1061
NmeAIII GCCGAG 1 cut(s) 492
NmuCI GTSAC 4 cut(s) 194, 247, 265, 452
PceI AGGCCT 1 cut(s) 78
PctI GAATGC 2 cut(s) 343, 607
PfeI GAWTC 5 cut(s) 88, 178, 693, 823, 1004
PkrI GCNGC 7 cut(s) 147, 156, 369, 631, 654, 657, 745
PleI GAGTC 2 cut(s) 200, 561
PmaCI CACGTG 1 cut(s) 270
PmlI CACGTG 1 cut(s) 270
PpsI GAGTC 2 cut(s) 200, 561
Ppu21I YACGTR 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 231
PspCI CACGTG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 234
PspN4I GGNNCC 4 cut(s) 149, 753, 942, 1061
PspPI GGNCC 5 cut(s) 58, 116, 139, 147, 902
PvuII CAGCTG 1 cut(s) 746
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 474, 777
SatI GCNGC 7 cut(s) 146, 155, 368, 630, 653, 656, 744
Sau3AI GATC 1 cut(s) 345
Sau96I GGNCC 5 cut(s) 58, 116, 139, 147, 902
SchI GAGTC 2 cut(s) 200, 561
ScrFI CCNGG 5 cut(s) 111, 142, 151, 564, 669
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 2 cut(s) 700, 792
SinI GGWCC 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 377
Sse9I AATT 7 cut(s) 298, 361, 484, 778, 918, 950, 1110
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 7 cut(s) 83, 114, 145, 155, 656, 713, 1013
SspMI CTAG 2 cut(s) 12, 845
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 5 cut(s) 109, 140, 149, 562, 667
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 1019
TaiI ACGT 1 cut(s) 272
TaqI TCGA 2 cut(s) 840, 994
TaqII GACCGA 1 cut(s) 233
TasI AATT 7 cut(s) 298, 361, 484, 778, 918, 950, 1110
TatI WGTACW 1 cut(s) 183
TauI GCSGC 3 cut(s) 148, 157, 658
TfiI GAWTC 5 cut(s) 88, 178, 693, 823, 1004
Tru1I TTAA 2 cut(s) 474, 777
Tru9I TTAA 2 cut(s) 474, 777
TscAI CASTG 5 cut(s) 102, 164, 197, 247, 912
TseFI GTSAC 4 cut(s) 194, 247, 265, 452
TseI GCWGC 4 cut(s) 367, 629, 652, 743
Tsp45I GTSAC 4 cut(s) 194, 247, 265, 452
TspDTI ATGAA 9 cut(s) 456, 614, 660, 747, 783, 905, 930, 1037, 1112
TspGWI ACGGA 1 cut(s) 1012
TspRI CASTG 5 cut(s) 102, 164, 197, 247, 912
VpaK11BI GGWCC 1 cut(s) 58
XapI RAATTY 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 2 cut(s) 12, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.