Rmu_sc0003711.1_g000003

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003711.1
OG0031029
Physical Location & Seq
Reverse (-)
5587 .. 12153
6567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003711.1_g000003.1.cds

Sequence Viewer

Length: 1482 bp
atggaatccgggcatcagctggctcgcctcttcacccttgtcacgtctgtggtggtgttttgcagcgatttggccggaagagctcgatttggagctgtgaaggttactgtagcaaatctggatttcagtcgatttccggcgattccggccttctccggccaccaaattggcgtcgaaggtgccgctgtcaaattttggtgtccatcggagatggcgccactgtgggcggcggtagcggtggctagggtttgggcttgggtcgttgggcttgggctcaactttggaatgatgggtgcttggtcctttgggcctaggtttgagctcaatcttaggctggttgagtttgctacagtggacaagaagagcaagaccaacctgttgccgaggttgttggctcctgaagaggctgaggacgtgttgacgtcgacgattctgcagcgtttcaggctgctacggcggctcagggcttgccggacggaggctggaggcatcccaggccattctgggcagcgatcgaggtcggaggaggtcgaggttgcaggtttctgggcagcgctgcaaccacgtcgccagcgactccaccgactgcaaaccggtccgtccgacccctggcctccatcctgcaagccccgccgctccgtcgcgcctcgagccgagctgcagcgttggcgtcctcactttagcgaaagtgcggacgtcctctttggaacgcctccacgatgcaccaaccgagtaccagagcgtggccctggtacttggggtcaccggaatcgtcggaaacagcctcgccgagtttctcccacttgccgatcgcgacactcccagcgggccgtggaaggtctacggcgtggcccggagaccccccgcccgcagtggaacgctgaccgcccgattcagtacatccagtgtgacctctctgactctaaacagacagaggcagagctgtcccagctgactgatgtcactcacattttttatgtgacatgggcgagcaagccaactgaagctgagaactgtgagactaatgggaagatgttgagcaatgtgttgaatgctgtgatcgcaaacgcgccgaatttgcagcacatatgtatgcagacgggtcggaagcactacttaggttcgtttgagatgttggggaatgtccaaccccatgaacctccatgtcaagaagacctgccgaggcttaatgccctcaatttttactatgtgatggagaatattctgtttgaggaggtgaagaaaaaggaggggcttacatggtttccggggagtaaaggaacttgggaaggattctgggacgcgtctgatgcggatatggttgctgaacatgagatatgggtagctgtggagccttatgccaagaatgaagcttttaattgcatcaatggggatttgttcaagtggaagcatttgtgggggattctggctgagcagttcgagctagtgcctgctggagttcacgaggagttgagtttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

493

Amino Acids

55.76

Weight (kDa)

8.84

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031029)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000010.1_g000026 Rmu_sc0003711.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 425, 699
Acc36I ACCTGC 2 cut(s) 530, 1185
AccB1I GGYRCC 2 cut(s) 179, 214
AccB7I CCANNNNNTGG 1 cut(s) 743
AccBSI CCGCTC 1 cut(s) 636
AccI GTMKAC 2 cut(s) 425, 841
AccII CGCG 4 cut(s) 644, 814, 1070, 1304
AcoI YGGCCR 2 cut(s) 72, 157
AcsI RAATTY 2 cut(s) 191, 1075
AcuI CTGAAG 2 cut(s) 420, 1023
AcyI GRCGYC 5 cut(s) 171, 215, 422, 670, 696
AfaI GTAC 3 cut(s) 735, 754, 899
AfeI AGCGCT 1 cut(s) 555
AfiI CCNNNNNNNGG 3 cut(s) 478, 609, 743
AflIII ACRYGT 2 cut(s) 414, 1302
AgeI ACCGGT 1 cut(s) 593
AgsI TTSAA 2 cut(s) 1051, 1402
AjiI CACGTC 3 cut(s) 45, 415, 566
AjnI CCWGG 3 cut(s) 493, 608, 748
AleI CACNNNNGTG 1 cut(s) 47
Alw21I GWGCWC 2 cut(s) 85, 324
Alw26I GTCTC 2 cut(s) 851, 1013
Ama87I CYCGRG 1 cut(s) 648
Aor51HI AGCGCT 1 cut(s) 555
AoxI GGCC 9 cut(s) 72, 147, 157, 308, 496, 611, 745, 828, 850
ApeKI GCWGC 9 cut(s) 63, 436, 448, 508, 551, 556, 658, 661, 1081
ApoI RAATTY 2 cut(s) 191, 1075
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AsiGI ACCGGT 1 cut(s) 593
AspA2I CCTAGG 1 cut(s) 311
AspLEI GCGC 4 cut(s) 217, 556, 646, 1072
AspS9I GGNCC 6 cut(s) 300, 308, 596, 746, 828, 851
AsuC2I CCSGG 3 cut(s) 10, 854, 1269
AsuHPI GGTGA 3 cut(s) 25, 755, 1249
AvaI CYCGRG 1 cut(s) 648
AvaII GGWCC 2 cut(s) 300, 596
AvrII CCTAGG 1 cut(s) 311
BanI GGYRCC 2 cut(s) 179, 214
BanII GRGCYC 3 cut(s) 85, 276, 324
BauI CACGAG 1 cut(s) 1463
BbsI GAAGAC 1 cut(s) 1179
Bbv12I GWGCWC 2 cut(s) 85, 324
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 9 cut(s) 75, 435, 448, 520, 543, 563, 645, 673, 1093
BccI CCATC 5 cut(s) 205, 211, 283, 625, 1207
BceAI ACGGC 3 cut(s) 470, 815, 860
BcgI CGANNNNNNTGC 6 cut(s) 415, 449, 548, 582, 1085, 1119
BciT130I CCWGG 3 cut(s) 495, 610, 750
BcnI CCSGG 3 cut(s) 10, 854, 1269
BcoDI GTCTC 2 cut(s) 851, 1013
BfaI CTAG 3 cut(s) 243, 312, 1445
BfmI CTRYAG 4 cut(s) 108, 348, 434, 659
BfoI RGCGCY 2 cut(s) 218, 557
BfuAI ACCTGC 2 cut(s) 530, 1185
BlnI CCTAGG 1 cut(s) 311
BlpI GCTNAGC 1 cut(s) 1431
Bme1390I CCNGG 6 cut(s) 10, 495, 610, 750, 854, 1269
Bme18I GGWCC 2 cut(s) 300, 596
BmeT110I CYCGRG 1 cut(s) 648
BmgBI CACGTC 3 cut(s) 45, 415, 566
BmgT120I GGNCC 6 cut(s) 300, 308, 596, 746, 828, 851
BmiI GGNNCC 4 cut(s) 181, 216, 396, 1353
BmrFI CCNGG 6 cut(s) 10, 495, 610, 750, 854, 1269
BmsI GCATC 5 cut(s) 22, 498, 710, 1300, 1392
BoxI GACNNNNGTC 1 cut(s) 959
BpiI GAAGAC 1 cut(s) 1179
BpmI CTGGAG 2 cut(s) 504, 1476
Bpu10I CCTNAGC 2 cut(s) 408, 461
Bpu1102I GCTNAGC 1 cut(s) 1431
BpuMI CCSGG 3 cut(s) 10, 854, 1269
BsaHI GRCGYC 5 cut(s) 171, 215, 422, 670, 696
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 8 cut(s) 311, 383, 493, 608, 748, 831, 1181, 1268
BsaWI WCCGGW 2 cut(s) 593, 765
Bsc4I CCNNNNNNNGG 3 cut(s) 478, 609, 743
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 904
Bse3DI GCAATG 1 cut(s) 1048
BseBI CCWGG 3 cut(s) 495, 610, 750
BseDI CCNNGG 8 cut(s) 311, 383, 493, 608, 748, 831, 1181, 1268
BseGI GGATG 3 cut(s) 489, 617, 900
BseLI CCNNNNNNNGG 3 cut(s) 478, 609, 743
BseMI GCAATG 1 cut(s) 1048
BseMII CTCAG 4 cut(s) 399, 475, 999, 1422
BseNI ACTGG 1 cut(s) 904
BseRI GAGGAG 3 cut(s) 539, 1247, 1481
BseXI GCAGC 9 cut(s) 75, 435, 448, 520, 543, 563, 645, 673, 1093
BseYI CCCAGC 2 cut(s) 822, 948
Bsh1236I CGCG 4 cut(s) 644, 814, 1070, 1304
Bsh1285I CGRYCG 2 cut(s) 515, 812
BshFI GGCC 9 cut(s) 74, 149, 159, 310, 498, 613, 747, 830, 852
BshNI GGYRCC 2 cut(s) 179, 214
BshTI ACCGGT 1 cut(s) 593
BsiEI CGRYCG 2 cut(s) 515, 812
BsiHKAI GWGCWC 2 cut(s) 85, 324
BsiHKCI CYCGRG 1 cut(s) 648
BslFI GGGAC 2 cut(s) 931, 1313
BslI CCNNNNNNNGG 3 cut(s) 478, 609, 743
BsmAI GTCTC 2 cut(s) 851, 1013
BsmFI GGGAC 2 cut(s) 931, 1313
BsmI GAATGC 1 cut(s) 1057
BsnI GGCC 9 cut(s) 74, 149, 159, 310, 498, 613, 747, 830, 852
Bso31I GGTCTC 1 cut(s) 851
BsoBI CYCGRG 1 cut(s) 648
Bsp1286I GDGCHC 3 cut(s) 85, 276, 324
Bsp143I GATC 3 cut(s) 512, 809, 1059
Bsp1720I GCTNAGC 1 cut(s) 1431
Bsp68I TCGCGA 1 cut(s) 814
BspANI GGCC 9 cut(s) 74, 149, 159, 310, 498, 613, 747, 830, 852
BspCNI CTCAG 4 cut(s) 400, 474, 1000, 1423
BspFNI CGCG 4 cut(s) 644, 814, 1070, 1304
BspLI GGNNCC 4 cut(s) 181, 216, 396, 1353
BspMAI CTGCAG 2 cut(s) 438, 663
BspMI ACCTGC 2 cut(s) 530, 1185
BspQI GCTCTTC 2 cut(s) 73, 356
BspT107I GGYRCC 2 cut(s) 179, 214
BspTNI GGTCTC 1 cut(s) 851
BsrBI CCGCTC 1 cut(s) 636
BsrDI GCAATG 1 cut(s) 1048
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 1 cut(s) 904
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 8 cut(s) 311, 383, 493, 608, 748, 831, 1181, 1268
BssMI GATC 3 cut(s) 512, 809, 1059
BssNI GRCGYC 5 cut(s) 171, 215, 422, 670, 696
BssSI CACGAG 1 cut(s) 1463
BssT1I CCWWGG 1 cut(s) 311
Bst2BI CACGAG 1 cut(s) 1463
Bst2UI CCWGG 3 cut(s) 495, 610, 750
Bst4CI ACNGT 4 cut(s) 109, 222, 352, 1016
Bst6I CTCTTC 4 cut(s) 35, 73, 356, 396
BstACI GRCGYC 5 cut(s) 171, 215, 422, 670, 696
BstDEI CTNAG 6 cut(s) 329, 408, 461, 1008, 1117, 1431
BstDSI CCRYGG 1 cut(s) 831
BstEII GGTNACC 1 cut(s) 761
BstF5I GGATG 3 cut(s) 489, 617, 900
BstFNI CGCG 4 cut(s) 644, 814, 1070, 1304
BstH2I RGCGCY 2 cut(s) 218, 557
BstHHI GCGC 4 cut(s) 217, 556, 646, 1072
BstKTI GATC 3 cut(s) 515, 812, 1062
BstMAI GTCTC 2 cut(s) 851, 1013
BstMBI GATC 3 cut(s) 512, 809, 1059
BstMCI CGRYCG 2 cut(s) 515, 812
BstNI CCWGG 3 cut(s) 495, 610, 750
BstPAI GACNNNNGTC 1 cut(s) 959
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 6 cut(s) 8, 493, 608, 748, 852, 1267
BstSFI CTRYAG 4 cut(s) 108, 348, 434, 659
BstUI CGCG 4 cut(s) 644, 814, 1070, 1304
BstV1I GCAGC 9 cut(s) 75, 435, 448, 520, 543, 563, 645, 673, 1093
BstV2I GAAGAC 1 cut(s) 1179
BstXI CCANNNNNNTGG 1 cut(s) 167
BsuRI GGCC 9 cut(s) 74, 149, 159, 310, 498, 613, 747, 830, 852
BtgI CCRYGG 1 cut(s) 831
BtrI CACGTC 3 cut(s) 45, 415, 566
BtsCI GGATG 3 cut(s) 489, 617, 900
BtsI GCAGTG 1 cut(s) 878
BtsIMutI CAGTG 4 cut(s) 218, 357, 878, 911
BtuMI TCGCGA 1 cut(s) 814
BveI ACCTGC 2 cut(s) 530, 1185
CfoI GCGC 4 cut(s) 217, 556, 646, 1072
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 6 cut(s) 300, 308, 596, 746, 828, 851
CpoI CGGWCCG 1 cut(s) 596
CseI GACGC 4 cut(s) 160, 659, 1293, 1310
Csp6I GTAC 3 cut(s) 734, 753, 898
CspAI ACCGGT 1 cut(s) 593
CspI CGGWCCG 1 cut(s) 596
CviAII CATG 5 cut(s) 984, 1154, 1164, 1260, 1331
CviQI GTAC 3 cut(s) 734, 753, 898
DdeI CTNAG 6 cut(s) 329, 408, 461, 1008, 1117, 1431
DinI GGCGCC 1 cut(s) 216
DpnI GATC 3 cut(s) 514, 811, 1061
DpnII GATC 3 cut(s) 512, 809, 1059
EaeI YGGCCR 2 cut(s) 72, 157
Eam1104I CTCTTC 4 cut(s) 35, 73, 356, 396
EarI CTCTTC 4 cut(s) 35, 73, 356, 396
Ecl136II GAGCTC 2 cut(s) 83, 322
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 3 cut(s) 85, 276, 324
Eco31I GGTCTC 1 cut(s) 851
Eco47I GGWCC 2 cut(s) 300, 596
Eco47III AGCGCT 1 cut(s) 555
Eco53kI GAGCTC 2 cut(s) 83, 322
Eco57I CTGAAG 2 cut(s) 420, 1023
Eco88I CYCGRG 1 cut(s) 648
Eco91I GGTNACC 1 cut(s) 761
EcoICRI GAGCTC 2 cut(s) 83, 322
EcoO65I GGTNACC 1 cut(s) 761
EcoRII CCWGG 3 cut(s) 493, 608, 748
EcoT14I CCWWGG 1 cut(s) 311
EcoT38I GRGCYC 3 cut(s) 85, 276, 324
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 5 cut(s) 987, 1157, 1167, 1263, 1334
FalI AAGNNNNNCTT 2 cut(s) 1100, 1132
FaqI GGGAC 2 cut(s) 931, 1313
FatI CATG 5 cut(s) 983, 1153, 1163, 1259, 1330
FauI CCCGC 4 cut(s) 638, 819, 872, 876
FauNDI CATATG 1 cut(s) 1088
FblI GTMKAC 2 cut(s) 425, 841
FokI GGATG 3 cut(s) 476, 604, 887
FriOI GRGCYC 3 cut(s) 85, 276, 324
FspBI CTAG 3 cut(s) 243, 312, 1445
GlaI GCGC 4 cut(s) 216, 555, 645, 1071
GsaI CCCAGC 2 cut(s) 826, 952
GsuI CTGGAG 2 cut(s) 504, 1476
HaeII RGCGCY 2 cut(s) 218, 557
HaeIII GGCC 9 cut(s) 74, 149, 159, 310, 498, 613, 747, 830, 852
HgaI GACGC 4 cut(s) 160, 659, 1293, 1310
HhaI GCGC 4 cut(s) 217, 556, 646, 1072
Hin1I GRCGYC 5 cut(s) 171, 215, 422, 670, 696
Hin1II CATG 5 cut(s) 987, 1157, 1167, 1263, 1334
Hin6I GCGC 4 cut(s) 215, 554, 644, 1070
HinP1I GCGC 4 cut(s) 215, 554, 644, 1070
HincII GTYRAC 2 cut(s) 420, 426
HindII GTYRAC 2 cut(s) 420, 426
HindIII AAGCTT 1 cut(s) 1371
HinfI GANTC 9 cut(s) 5, 142, 430, 576, 769, 892, 920, 1293, 1423
HphI GGTGA 3 cut(s) 25, 755, 1249
Hpy166II GTNNAC 5 cut(s) 355, 420, 426, 842, 1462
Hpy188I TCNGA 7 cut(s) 208, 523, 604, 777, 919, 1107, 1309
Hpy188III TCNNGA 5 cut(s) 119, 398, 813, 1169, 1463
Hpy8I GTNNAC 5 cut(s) 355, 420, 426, 842, 1462
Hpy99I CGWCG 6 cut(s) 176, 427, 430, 570, 644, 777
HpyAV CCTTC 5 cut(s) 94, 160, 170, 830, 1283
HpyCH4III ACNGT 4 cut(s) 109, 222, 352, 1016
HpyCH4IV ACGT 5 cut(s) 44, 414, 422, 565, 696
HpyF3I CTNAG 6 cut(s) 329, 408, 461, 1008, 1117, 1431
HpySE526I ACGT 5 cut(s) 44, 414, 422, 565, 696
Hsp92I GRCGYC 5 cut(s) 171, 215, 422, 670, 696
Hsp92II CATG 5 cut(s) 987, 1157, 1167, 1263, 1334
HspAI GCGC 4 cut(s) 215, 554, 644, 1070
KasI GGCGCC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 512, 809, 1059
LguI GCTCTTC 2 cut(s) 73, 356
LmnI GCTCC 4 cut(s) 92, 400, 641, 1351
Lsp1109I GCAGC 9 cut(s) 75, 435, 448, 520, 543, 563, 645, 673, 1093
LweI GCATC 5 cut(s) 22, 498, 710, 1300, 1392
MaeI CTAG 3 cut(s) 243, 312, 1445
MaeII ACGT 5 cut(s) 44, 414, 422, 565, 696
MaeIII GTNAC 6 cut(s) 40, 103, 761, 908, 961, 979
MalI GATC 3 cut(s) 514, 811, 1061
MbiI CCGCTC 1 cut(s) 636
MboI GATC 3 cut(s) 512, 809, 1059
MboII GAAGA 7 cut(s) 22, 90, 373, 413, 1042, 1184, 1252
MhlI GDGCHC 3 cut(s) 85, 276, 324
MluCI AATT 5 cut(s) 165, 191, 1075, 1198, 1378
MluI ACGCGT 1 cut(s) 1302
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 2 cut(s) 570, 914
MmeI TCCRAC 5 cut(s) 501, 627, 755, 1085, 1171
MseI TTAA 2 cut(s) 1188, 1377
MslI CAYNNNNRTG 2 cut(s) 47, 1091
MspA1I CMGCKG 4 cut(s) 19, 185, 826, 952
MspR9I CCNGG 6 cut(s) 10, 495, 610, 750, 854, 1269
Mva1269I GAATGC 1 cut(s) 1057
MvaI CCWGG 3 cut(s) 495, 610, 750
MvnI CGCG 4 cut(s) 644, 814, 1070, 1304
NarI GGCGCC 1 cut(s) 215
NciI CCSGG 3 cut(s) 10, 854, 1269
NdeI CATATG 1 cut(s) 1088
NdeII GATC 3 cut(s) 512, 809, 1059
NlaIII CATG 5 cut(s) 987, 1157, 1167, 1263, 1334
NlaIV GGNNCC 4 cut(s) 181, 216, 396, 1353
NmeAIII GCCGAG 4 cut(s) 408, 679, 815, 1206
NmuCI GTSAC 5 cut(s) 40, 761, 908, 961, 979
NruI TCGCGA 1 cut(s) 814
OliI CACNNNNGTG 1 cut(s) 47
PaeR7I CTCGAG 1 cut(s) 648
PciSI GCTCTTC 2 cut(s) 73, 356
PcsI WCGNNNNNNNCGW 1 cut(s) 571
PctI GAATGC 1 cut(s) 1057
PfeI GAWTC 7 cut(s) 5, 142, 430, 769, 892, 1293, 1423
PflMI CCANNNNNTGG 1 cut(s) 743
PinAI ACCGGT 1 cut(s) 593
Ple19I CGATCG 2 cut(s) 515, 812
PleI GAGTC 2 cut(s) 570, 914
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 2 cut(s) 570, 914
PshAI GACNNNNGTC 1 cut(s) 959
Psp124BI GAGCTC 2 cut(s) 85, 324
Psp6I CCWGG 3 cut(s) 493, 608, 748
PspEI GGTNACC 1 cut(s) 761
PspFI CCCAGC 2 cut(s) 822, 948
PspGI CCWGG 3 cut(s) 493, 608, 748
PspN4I GGNNCC 4 cut(s) 181, 216, 396, 1353
PspPI GGNCC 6 cut(s) 300, 308, 596, 746, 828, 851
PspXI VCTCGAGB 1 cut(s) 648
PstI CTGCAG 2 cut(s) 438, 663
PvuI CGATCG 2 cut(s) 515, 812
PvuII CAGCTG 2 cut(s) 19, 952
RruI TCGCGA 1 cut(s) 814
RsaI GTAC 3 cut(s) 735, 754, 899
RsaNI GTAC 3 cut(s) 734, 753, 898
RseI CAYNNNNRTG 2 cut(s) 47, 1091
Rsr2I CGGWCCG 1 cut(s) 596
RsrII CGGWCCG 1 cut(s) 596
SacI GAGCTC 2 cut(s) 85, 324
SalI GTCGAC 1 cut(s) 424
SapI GCTCTTC 2 cut(s) 73, 356
SaqAI TTAA 2 cut(s) 1188, 1377
Sau3AI GATC 3 cut(s) 512, 809, 1059
Sau96I GGNCC 6 cut(s) 300, 308, 596, 746, 828, 851
SchI GAGTC 2 cut(s) 570, 914
ScrFI CCNGG 6 cut(s) 10, 495, 610, 750, 854, 1269
SduI GDGCHC 3 cut(s) 85, 276, 324
SfaNI GCATC 5 cut(s) 22, 498, 710, 1300, 1392
SfcI CTRYAG 4 cut(s) 108, 348, 434, 659
SfoI GGCGCC 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 648
SgrDI CGTCGACG 1 cut(s) 424
SinI GGWCC 2 cut(s) 300, 596
SlaI CTCGAG 1 cut(s) 648
SmiMI CAYNNNNRTG 2 cut(s) 47, 1091
SmlI CTYRAG 1 cut(s) 648
SmoI CTYRAG 1 cut(s) 648
Sse9I AATT 5 cut(s) 165, 191, 1075, 1198, 1378
SspDI GGCGCC 1 cut(s) 214
SspI AATATT 1 cut(s) 1222
SspMI CTAG 3 cut(s) 243, 312, 1445
SstI GAGCTC 2 cut(s) 85, 324
StyD4I CCNGG 6 cut(s) 8, 493, 608, 748, 852, 1267
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 4 cut(s) 109, 222, 352, 1016
TaiI ACGT 5 cut(s) 47, 417, 425, 568, 699
TaqI TCGA 8 cut(s) 85, 130, 174, 425, 515, 531, 649, 1440
TasI AATT 5 cut(s) 165, 191, 1075, 1198, 1378
TatI WGTACW 1 cut(s) 897
TauI GCSGC 4 cut(s) 185, 230, 460, 636
TfiI GAWTC 7 cut(s) 5, 142, 430, 769, 892, 1293, 1423
Tru1I TTAA 2 cut(s) 1188, 1377
Tru9I TTAA 2 cut(s) 1188, 1377
TscAI CASTG 4 cut(s) 225, 357, 878, 911
TseFI GTSAC 5 cut(s) 40, 761, 908, 961, 979
TseI GCWGC 9 cut(s) 63, 436, 448, 508, 551, 556, 658, 661, 1081
Tsp45I GTSAC 5 cut(s) 40, 761, 908, 961, 979
TspDTI ATGAA 2 cut(s) 1170, 1383
TspGWI ACGGA 3 cut(s) 491, 588, 628
TspRI CASTG 4 cut(s) 225, 357, 878, 911
Van91I CCANNNNNTGG 1 cut(s) 743
VpaK11BI GGWCC 2 cut(s) 300, 596
XapI RAATTY 2 cut(s) 191, 1075
XhoI CTCGAG 1 cut(s) 648
XmaJI CCTAGG 1 cut(s) 311
XmiI GTMKAC 2 cut(s) 425, 841
XspI CTAG 3 cut(s) 243, 312, 1445
ZraI GACGTC 2 cut(s) 423, 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.