Rmu_sc0000084.1_g000052
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000084.1
OG0031038
Physical Location & Seq
Forward (+)
225295 .. 226619
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
atgatggtgtcggcgtcgccgtcgatggtggcgaggagctccttggaggaaatgctggattcgctgcggcggagggacgaggctgagaagcccaaggaattgccgccggcattgccgtcccggcccacttccaaggctcggctgccgtcggcgcgccgctccttgcccaacaacttcaatgccgaggccttaatccggtcgccggagtgggcccccagtgtgaacaagaggaaagagagggattccggggtcaagaggtgtaataatttttgtgtcaagaagacggagaaggacgcggtggagtcgccgtacgttgcgtcggaggaggagcttcagagccggcaaaaggtggcgaagtccgattggggtgacaacgttgcttatttcatcatgaagaaacttaaggtttggtgtcggggtcctagtggactatgggagtcaggaacaatacaatcaacgtccggggatgtggcatttgtgtcactttcaagtggaaatgtaatcaaagtgcccagggatgagctgctaccagcaaacccagatgttctggaaggggtggatgatcttgcacagcttagctatttaaatgacccttctgttctttgcaatcttcagtctagatattcgcaggacatgatttatagcaaagcagggccagttttgattgcaatgaatcctttcaaagatgtccaaatttgtggagatgattttgtcacagcgtataggaagaaacttgtggacaaacctcatgtttatgcaatagcaaacgctgcttataatgagatgatgagaggtgtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009506 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0012505 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030054 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0055044 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_N Myosin_head

Protein Analysis

266

Amino Acids

29.62

Weight (kDa)

8.7

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_VIII-1_N PF25369 134 - 178 2.4e-18 Myosin-1-like, N-terminal SH3 domain
Myosin_head PF00063 186 - 263 5e-22 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031038)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8314189.1_g000001 Rmu_sc0000084.1_g000052

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 777
AccBSI CCGCTC 1 cut(s) 159
AccII CGCG 2 cut(s) 154, 296
AciI CCGC 5 cut(s) 67, 70, 104, 157, 296
AclI AACGTT 1 cut(s) 375
AcsI RAATTY 1 cut(s) 693
AcuI CTGAAG 2 cut(s) 317, 596
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 4 cut(s) 138, 195, 202, 346
AflII CTTAAG 1 cut(s) 401
AgsI TTSAA 3 cut(s) 178, 489, 682
AjnI CCWGG 1 cut(s) 512
AluBI AGCT 5 cut(s) 39, 331, 523, 574, 579
AluI AGCT 5 cut(s) 39, 331, 523, 574, 579
Alw21I GWGCWC 1 cut(s) 41
AoxI GGCC 4 cut(s) 122, 186, 210, 653
ApaI GGGCCC 1 cut(s) 214
ApeKI GCWGC 4 cut(s) 64, 142, 523, 770
ApoI RAATTY 1 cut(s) 693
AscI GGCGCGCC 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 677
AspLEI GCGC 2 cut(s) 154, 156
AspS9I GGNCC 5 cut(s) 123, 210, 211, 419, 653
AsuC2I CCSGG 3 cut(s) 121, 247, 463
AsuHPI GGTGA 1 cut(s) 380
AvaII GGWCC 1 cut(s) 419
BaeGI GKGCMC 2 cut(s) 214, 513
BanII GRGCYC 2 cut(s) 41, 214
BbsI GAAGAC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 4 cut(s) 51, 129, 510, 757
BccI CCATC 1 cut(s) 19
BceAI ACGGC 4 cut(s) 4, 100, 130, 292
BciT130I CCWGG 1 cut(s) 514
BcnI CCSGG 3 cut(s) 121, 247, 463
BfaI CTAG 2 cut(s) 423, 618
BfrI CTTAAG 1 cut(s) 401
BglI GCCNNNNNGGC 1 cut(s) 121
BisI GCNGC 7 cut(s) 65, 68, 104, 143, 157, 524, 771
BlpI GCTNAGC 1 cut(s) 575
BlsI GCNGC 7 cut(s) 66, 69, 105, 144, 158, 525, 772
Bme1390I CCNGG 4 cut(s) 121, 247, 463, 514
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 5 cut(s) 123, 210, 211, 419, 653
BmiI GGNNCC 3 cut(s) 212, 213, 420
BmrFI CCNGG 4 cut(s) 121, 247, 463, 514
BmrI ACTGGG 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 287
Bpu1102I GCTNAGC 1 cut(s) 575
BpuMI CCSGG 3 cut(s) 121, 247, 463
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 8 cut(s) 42, 93, 132, 183, 246, 462, 512, 513
BsaWI WCCGGW 1 cut(s) 195
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 195, 202, 346
Bse118I RCCGGY 2 cut(s) 106, 339
Bse1I ACTGG 2 cut(s) 216, 656
Bse3DI GCAATG 2 cut(s) 110, 675
BseBI CCWGG 1 cut(s) 514
BseDI CCNNGG 8 cut(s) 42, 93, 132, 183, 246, 462, 512, 513
BseGI GGATG 3 cut(s) 472, 523, 565
BseLI CCNNNNNNNGG 4 cut(s) 138, 195, 202, 346
BseMI GCAATG 2 cut(s) 110, 675
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 2 cut(s) 216, 656
BsePI GCGCGC 1 cut(s) 152
BseRI GAGGAG 3 cut(s) 49, 338, 341
BseSI GKGCMC 2 cut(s) 214, 513
BseXI GCAGC 4 cut(s) 51, 129, 510, 757
Bsh1236I CGCG 2 cut(s) 154, 296
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 4 cut(s) 124, 188, 212, 655
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 7 cut(s) 107, 121, 196, 203, 246, 340, 462
BsiWI CGTACG 1 cut(s) 309
BslFI GGGAC 2 cut(s) 89, 103
BslI CCNNNNNNNGG 4 cut(s) 138, 195, 202, 346
BsmFI GGGAC 2 cut(s) 89, 103
BsnI GGCC 4 cut(s) 124, 188, 212, 655
Bsp120I GGGCCC 1 cut(s) 210
Bsp1286I GDGCHC 3 cut(s) 41, 214, 513
Bsp143I GATC 1 cut(s) 562
Bsp1720I GCTNAGC 1 cut(s) 575
BspACI CCGC 5 cut(s) 67, 70, 104, 157, 296
BspANI GGCC 4 cut(s) 124, 188, 212, 655
BspCNI CTCAG 1 cut(s) 76
BspFNI CGCG 2 cut(s) 154, 296
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 3 cut(s) 212, 213, 420
BspTI CTTAAG 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 159
BsrDI GCAATG 2 cut(s) 110, 675
BsrFI RCCGGY 2 cut(s) 106, 339
BsrI ACTGG 2 cut(s) 216, 656
BssAI RCCGGY 2 cut(s) 106, 339
BssECI CCNNGG 8 cut(s) 42, 93, 132, 183, 246, 462, 512, 513
BssHII GCGCGC 1 cut(s) 152
BssMI GATC 1 cut(s) 562
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 3 cut(s) 42, 93, 132
Bst2UI CCWGG 1 cut(s) 514
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 770
BstC8I GCNNGC 3 cut(s) 108, 154, 341
BstDEI CTNAG 2 cut(s) 84, 575
BstF5I GGATG 3 cut(s) 472, 523, 565
BstFNI CGCG 2 cut(s) 154, 296
BstHHI GCGC 2 cut(s) 154, 156
BstKTI GATC 1 cut(s) 565
BstMBI GATC 1 cut(s) 562
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 5 cut(s) 61, 112, 121, 151, 770
BstNI CCWGG 1 cut(s) 514
BstSCI CCNGG 4 cut(s) 119, 245, 461, 512
BstSLI GKGCMC 2 cut(s) 214, 513
BstUI CGCG 2 cut(s) 154, 296
BstV1I GCAGC 4 cut(s) 51, 129, 510, 757
BstV2I GAAGAC 1 cut(s) 287
BstXI CCANNNNNNTGG 1 cut(s) 698
BsuRI GGCC 4 cut(s) 124, 188, 212, 655
BtsCI GGATG 3 cut(s) 472, 523, 565
BtsIMutI CAGTG 1 cut(s) 223
Cac8I GCNNGC 3 cut(s) 108, 154, 341
CciI TCATGA 1 cut(s) 390
CfoI GCGC 2 cut(s) 154, 156
Cfr10I RCCGGY 2 cut(s) 106, 339
Cfr13I GGNCC 5 cut(s) 123, 210, 211, 419, 653
CseI GACGC 3 cut(s) 3, 302, 306
Csp6I GTAC 1 cut(s) 310
CviAII CATG 3 cut(s) 391, 634, 749
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 2 cut(s) 84, 575
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
DraI TTTAAA 1 cut(s) 585
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 39
Eco130I CCWWGG 3 cut(s) 42, 93, 132
Eco147I AGGCCT 1 cut(s) 188
Eco24I GRGCYC 2 cut(s) 41, 214
Eco47I GGWCC 1 cut(s) 419
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 2 cut(s) 317, 596
EcoICRI GAGCTC 1 cut(s) 39
EcoO109I RGGNCCY 2 cut(s) 211, 419
EcoRII CCWGG 1 cut(s) 512
EcoT14I CCWWGG 3 cut(s) 42, 93, 132
EcoT38I GRGCYC 2 cut(s) 41, 214
ErhI CCWWGG 3 cut(s) 42, 93, 132
FaeI CATG 3 cut(s) 394, 637, 752
FaiI YATR 9 cut(s) 392, 433, 635, 642, 723, 750, 756, 777, 799
FaqI GGGAC 2 cut(s) 89, 103
FatI CATG 3 cut(s) 390, 633, 748
Fnu4HI GCNGC 7 cut(s) 65, 68, 104, 143, 157, 524, 771
FokI GGATG 3 cut(s) 479, 530, 572
FriOI GRGCYC 2 cut(s) 41, 214
Fsp4HI GCNGC 7 cut(s) 65, 68, 104, 143, 157, 524, 771
FspBI CTAG 2 cut(s) 423, 618
GlaI GCGC 2 cut(s) 153, 155
GluI GCNGC 7 cut(s) 65, 68, 104, 143, 157, 524, 771
HaeIII GGCC 4 cut(s) 124, 188, 212, 655
HapII CCGG 7 cut(s) 107, 121, 196, 203, 246, 340, 462
HgaI GACGC 3 cut(s) 3, 302, 306
HhaI GCGC 2 cut(s) 154, 156
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 3 cut(s) 394, 637, 752
Hin6I GCGC 2 cut(s) 152, 154
HinP1I GCGC 2 cut(s) 152, 154
HinfI GANTC 5 cut(s) 59, 242, 302, 437, 673
HpaII CCGG 7 cut(s) 107, 121, 196, 203, 246, 340, 462
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 3 cut(s) 223, 428, 739
Hpy188I TCNGA 3 cut(s) 322, 336, 361
Hpy188III TCNNGA 6 cut(s) 253, 277, 391, 441, 548, 618
Hpy8I GTNNAC 3 cut(s) 223, 428, 739
Hpy99I CGWCG 4 cut(s) 19, 25, 151, 322
HpyAV CCTTC 3 cut(s) 283, 545, 603
HpyCH4IV ACGT 3 cut(s) 312, 375, 458
HpyCH4V TGCA 4 cut(s) 569, 606, 668, 758
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 112, 121, 151, 770
HpyF3I CTNAG 2 cut(s) 84, 575
HpySE526I ACGT 3 cut(s) 312, 375, 458
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 3 cut(s) 394, 637, 752
HspAI GCGC 2 cut(s) 152, 154
KroI GCCGGC 2 cut(s) 106, 339
KroNI GCCGGC 2 cut(s) 108, 341
Kzo9I GATC 1 cut(s) 562
LmnI GCTCC 4 cut(s) 36, 44, 164, 328
Lsp1109I GCAGC 4 cut(s) 51, 129, 510, 757
MaeI CTAG 2 cut(s) 423, 618
MaeII ACGT 3 cut(s) 312, 375, 458
MaeIII GTNAC 3 cut(s) 368, 480, 712
MalI GATC 1 cut(s) 564
MbiI CCGCTC 1 cut(s) 159
MboI GATC 1 cut(s) 562
MboII GAAGA 4 cut(s) 292, 406, 602, 739
MhlI GDGCHC 3 cut(s) 41, 214, 513
MluCI AATT 3 cut(s) 98, 265, 693
MlyI GAGTC 2 cut(s) 311, 446
MmeI TCCRAC 1 cut(s) 300
MroNI GCCGGC 2 cut(s) 106, 339
MroXI GAANNNNTTC 1 cut(s) 677
MseI TTAA 3 cut(s) 191, 402, 584
MslI CAYNNNNRTG 1 cut(s) 753
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 7 cut(s) 107, 121, 196, 203, 246, 340, 462
MspR9I CCNGG 4 cut(s) 121, 247, 463, 514
MvaI CCWGG 1 cut(s) 514
MvnI CGCG 2 cut(s) 154, 296
MwoI GCNNNNNNNGC 5 cut(s) 61, 112, 121, 151, 770
NaeI GCCGGC 2 cut(s) 108, 341
NciI CCSGG 3 cut(s) 121, 247, 463
NdeII GATC 1 cut(s) 562
NgoMIV GCCGGC 2 cut(s) 106, 339
NlaIII CATG 3 cut(s) 394, 637, 752
NlaIV GGNNCC 3 cut(s) 212, 213, 420
NmeAIII GCCGAG 2 cut(s) 118, 208
NmuCI GTSAC 3 cut(s) 368, 480, 712
PagI TCATGA 1 cut(s) 390
PalAI GGCGCGCC 1 cut(s) 152
PasI CCCWGGG 1 cut(s) 513
PauI GCGCGC 1 cut(s) 152
PceI AGGCCT 1 cut(s) 188
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 29
PdiI GCCGGC 2 cut(s) 108, 341
PdmI GAANNNNTTC 1 cut(s) 677
PfeI GAWTC 3 cut(s) 59, 242, 673
Pfl23II CGTACG 1 cut(s) 309
PkrI GCNGC 7 cut(s) 66, 69, 105, 144, 158, 525, 772
PleI GAGTC 2 cut(s) 310, 445
PpsI GAGTC 2 cut(s) 310, 445
PpuMI RGGWCCY 1 cut(s) 419
PsiI TTATAA 1 cut(s) 777
Psp124BI GAGCTC 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 375
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 1 cut(s) 512
PspGI CCWGG 1 cut(s) 512
PspLI CGTACG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 212, 213, 420
PspOMI GGGCCC 1 cut(s) 210
PspPI GGNCC 5 cut(s) 123, 210, 211, 419, 653
PspPPI RGGWCCY 1 cut(s) 419
PteI GCGCGC 1 cut(s) 152
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 753
SacI GAGCTC 1 cut(s) 41
SaqAI TTAA 3 cut(s) 191, 402, 584
SatI GCNGC 7 cut(s) 65, 68, 104, 143, 157, 524, 771
Sau3AI GATC 1 cut(s) 562
Sau96I GGNCC 5 cut(s) 123, 210, 211, 419, 653
SchI GAGTC 2 cut(s) 311, 446
ScrFI CCNGG 4 cut(s) 121, 247, 463, 514
SduI GDGCHC 3 cut(s) 41, 214, 513
SgsI GGCGCGCC 1 cut(s) 152
SinI GGWCC 1 cut(s) 419
SmiI ATTTAAAT 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 3 cut(s) 98, 265, 693
SseBI AGGCCT 1 cut(s) 188
SsiI CCGC 5 cut(s) 67, 70, 104, 157, 296
SspMI CTAG 2 cut(s) 423, 618
SstI GAGCTC 1 cut(s) 41
StuI AGGCCT 1 cut(s) 188
StyD4I CCNGG 4 cut(s) 119, 245, 461, 512
StyI CCWWGG 3 cut(s) 42, 93, 132
SwaI ATTTAAAT 1 cut(s) 585
TaiI ACGT 3 cut(s) 315, 378, 461
TaqI TCGA 1 cut(s) 23
TasI AATT 3 cut(s) 98, 265, 693
TauI GCSGC 3 cut(s) 70, 106, 159
TfiI GAWTC 3 cut(s) 59, 242, 673
Tru1I TTAA 3 cut(s) 191, 402, 584
Tru9I TTAA 3 cut(s) 191, 402, 584
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 3 cut(s) 368, 480, 712
TseI GCWGC 4 cut(s) 64, 142, 523, 770
Tsp45I GTSAC 3 cut(s) 368, 480, 712
TspDTI ATGAA 3 cut(s) 376, 407, 686
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 1 cut(s) 223
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 1 cut(s) 693
XbaI TCTAGA 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 677
XspI CTAG 2 cut(s) 423, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.