Rmu_sc0000284.1_g000024

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000284.1
OG0031058
Physical Location & Seq
Forward (+)
94133 .. 97750
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
atggtcttttacagaactggctcggctcgagccgacactgttactaagtcgatcaacaatgtggtccagcaacgccctctacatgatgttctcgtacaacaactacggcaaagacaccagcctctgggatcaggcgaagacggagcgagacgtgttctgggagcggggtcaagttcgagttcgggtttttcaggcggcatgccgaagaatctaaaggctgacgtgaccatgtgtaaggatgggtgtgagtaccggacggtgcaggaggaggtggataaggcgccggagaatttggaggtagggaggaggtttgtgtttcagataaagatgggggtgtacgaggagactgtgagtggctttggagaagcagatcctggtgttcttggtattggtcaatggaataatctcagtcggagtggaggctctgtggcggctttgtcatccaaaacgcgaggaggtatcgccggggaagtccagagccagttcggtggatcagagtttttggatctggtttgtgtcgtttggagccgccctgtggagaggggtagttggggatggcagtgctgcatgctggcgtttggggagggcggagagatgatgggatggtctggttttgctcaagccggcggtttggatgctggtggttggacagactggttccatcttgcgggacggtggcgaggtgaccgcagcagcttgtacttttggactgggctgagcaggggctag

Protein Analysis

242

Amino Acids

26.19

Weight (kDa)

6.83

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031058)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000284.1_g000024
rosa_roxburghii Rroxscaffold_4G00311650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 280
AccB7I CCANNNNNTGG 1 cut(s) 124
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 1 cut(s) 451
AciI CCGC 8 cut(s) 164, 195, 431, 529, 588, 627, 668, 688
AclWI GGATC 4 cut(s) 136, 365, 499, 513
AcsI RAATTY 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 281
AfaI GTAC 4 cut(s) 96, 251, 338, 701
AfiI CCNNNNNNNGG 3 cut(s) 124, 535, 667
AflIII ACRYGT 1 cut(s) 151
AjiI CACGTC 2 cut(s) 152, 223
AjnI CCWGG 1 cut(s) 373
AluBI AGCT 1 cut(s) 696
AluI AGCT 1 cut(s) 696
Alw26I GTCTC 2 cut(s) 142, 338
AlwI GGATC 4 cut(s) 136, 365, 499, 513
AlwNI CAGNNNCTG 2 cut(s) 124, 374
Ama87I CYCGRG 1 cut(s) 27
ApeKI GCWGC 3 cut(s) 564, 690, 693
ApoI RAATTY 1 cut(s) 289
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 695
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 1 cut(s) 64
BanI GGYRCC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 551, 702, 705
BccI CCATC 6 cut(s) 233, 322, 549, 592, 597, 669
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 375
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 2 cut(s) 142, 338
BfaI CTAG 1 cut(s) 727
BfoI RGCGCY 1 cut(s) 284
BisI GCNGC 6 cut(s) 196, 432, 529, 565, 691, 694
BlpI GCTNAGC 1 cut(s) 716
BlsI GCNGC 6 cut(s) 197, 433, 530, 566, 692, 695
Bme1390I CCNGG 2 cut(s) 375, 466
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 1 cut(s) 27
BmgBI CACGTC 2 cut(s) 152, 223
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 3 cut(s) 282, 527, 659
BmrFI CCNGG 2 cut(s) 375, 466
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 144
Bpu1102I GCTNAGC 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 603
BpuMI CCSGG 1 cut(s) 466
BsaHI GRCGYC 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 465
BsaWI WCCGGW 1 cut(s) 252
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 535, 667
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 4 cut(s) 22, 481, 659, 715
BseBI CCWGG 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 5 cut(s) 244, 440, 560, 608, 640
BseLI CCNNNNNNNGG 3 cut(s) 124, 535, 667
BseMII CTCAG 2 cut(s) 421, 707
BseNI ACTGG 4 cut(s) 22, 481, 659, 715
BseRI GAGGAG 4 cut(s) 281, 319, 356, 468
BseXI GCAGC 3 cut(s) 551, 702, 705
BsgI GTGCAG 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 451
BshNI GGYRCC 1 cut(s) 280
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 4 cut(s) 253, 284, 465, 624
BslFI GGGAC 1 cut(s) 684
BslI CCNNNNNNNGG 3 cut(s) 124, 535, 667
BsmAI GTCTC 2 cut(s) 142, 338
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 684
BsoBI CYCGRG 1 cut(s) 27
Bsp143I GATC 5 cut(s) 51, 128, 370, 491, 505
Bsp1720I GCTNAGC 1 cut(s) 716
BspACI CCGC 8 cut(s) 164, 195, 431, 529, 588, 627, 668, 688
BspCNI CTCAG 2 cut(s) 420, 708
BspFNI CGCG 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 282, 527, 659
BspPI GGATC 4 cut(s) 136, 365, 499, 513
BspT107I GGYRCC 1 cut(s) 280
BsrBI CCGCTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 623
BsrI ACTGG 4 cut(s) 22, 481, 659, 715
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 5 cut(s) 51, 128, 370, 491, 505
BssNI GRCGYC 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 4 cut(s) 40, 259, 349, 675
BstACI GRCGYC 1 cut(s) 281
BstC8I GCNNGC 4 cut(s) 200, 569, 573, 625
BstDEI CTNAG 3 cut(s) 45, 407, 716
BstEII GGTNACC 1 cut(s) 683
BstF5I GGATG 5 cut(s) 244, 440, 560, 608, 640
BstFNI CGCG 1 cut(s) 451
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 5 cut(s) 54, 131, 373, 494, 508
BstMAI GTCTC 2 cut(s) 142, 338
BstMBI GATC 5 cut(s) 51, 128, 370, 491, 505
BstNI CCWGG 1 cut(s) 375
BstNSI RCATGY 2 cut(s) 202, 571
BstPI GGTNACC 1 cut(s) 683
BstSCI CCNGG 2 cut(s) 373, 464
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 3 cut(s) 551, 702, 705
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 2 cut(s) 370, 505
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 2 cut(s) 370, 505
BtrI CACGTC 2 cut(s) 152, 223
BtsCI GGATG 5 cut(s) 244, 440, 560, 608, 640
BtsI GCAGTG 1 cut(s) 566
BtsIMutI CAGTG 2 cut(s) 36, 566
Cac8I GCNNGC 4 cut(s) 200, 569, 573, 625
CaiI CAGNNNCTG 2 cut(s) 124, 374
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 1 cut(s) 64
Csp6I GTAC 4 cut(s) 95, 250, 337, 700
CviAII CATG 4 cut(s) 83, 199, 229, 568
CviQI GTAC 4 cut(s) 95, 250, 337, 700
DdeI CTNAG 3 cut(s) 45, 407, 716
DinI GGCGCC 1 cut(s) 282
DpnI GATC 5 cut(s) 53, 130, 372, 493, 507
DpnII GATC 5 cut(s) 51, 128, 370, 491, 505
EciI GGCGGA 1 cut(s) 603
Eco47I GGWCC 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 683
EcoO65I GGTNACC 1 cut(s) 683
EcoRII CCWGG 1 cut(s) 373
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 142
FaeI CATG 4 cut(s) 86, 202, 232, 571
FaiI YATR 4 cut(s) 84, 200, 230, 569
FaqI GGGAC 1 cut(s) 684
FatI CATG 4 cut(s) 82, 198, 228, 567
FauI CCCGC 2 cut(s) 157, 661
Fnu4HI GCNGC 6 cut(s) 196, 432, 529, 565, 691, 694
FokI GGATG 5 cut(s) 251, 427, 567, 615, 647
Fsp4HI GCNGC 6 cut(s) 196, 432, 529, 565, 691, 694
FspBI CTAG 1 cut(s) 727
GlaI GCGC 1 cut(s) 282
GluI GCNGC 6 cut(s) 196, 432, 529, 565, 691, 694
HaeII RGCGCY 1 cut(s) 284
HapII CCGG 4 cut(s) 253, 284, 465, 624
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 4 cut(s) 86, 202, 232, 571
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 1 cut(s) 208
HpaII CCGG 4 cut(s) 253, 284, 465, 624
HphI GGTGA 1 cut(s) 695
Hpy166II GTNNAC 1 cut(s) 337
Hpy188I TCNGA 3 cut(s) 321, 414, 496
Hpy188III TCNNGA 1 cut(s) 475
Hpy8I GTNNAC 1 cut(s) 337
HpyCH4III ACNGT 4 cut(s) 40, 259, 349, 675
HpyCH4IV ACGT 2 cut(s) 151, 222
HpyCH4V TGCA 2 cut(s) 262, 567
HpyF3I CTNAG 3 cut(s) 45, 407, 716
HpySE526I ACGT 2 cut(s) 151, 222
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 4 cut(s) 86, 202, 232, 571
HspAI GCGC 1 cut(s) 281
KasI GGCGCC 1 cut(s) 280
KroI GCCGGC 1 cut(s) 623
KroNI GCCGGC 1 cut(s) 625
Kzo9I GATC 5 cut(s) 51, 128, 370, 491, 505
LmnI GCTCC 3 cut(s) 143, 161, 525
Lsp1109I GCAGC 3 cut(s) 551, 702, 705
LweI GCATC 1 cut(s) 625
MaeI CTAG 1 cut(s) 727
MaeII ACGT 2 cut(s) 151, 222
MaeIII GTNAC 3 cut(s) 40, 223, 683
MalI GATC 5 cut(s) 53, 130, 372, 493, 507
MbiI CCGCTC 1 cut(s) 164
MboI GATC 5 cut(s) 51, 128, 370, 491, 505
MboII GAAGA 2 cut(s) 149, 217
MflI RGATCY 2 cut(s) 370, 505
MluCI AATT 1 cut(s) 289
Mly113I GGCGCC 1 cut(s) 281
MmeI TCCRAC 2 cut(s) 392, 626
MroNI GCCGGC 1 cut(s) 623
MspI CCGG 4 cut(s) 253, 284, 465, 624
MspR9I CCNGG 2 cut(s) 375, 466
MvaI CCWGG 1 cut(s) 375
MvnI CGCG 1 cut(s) 451
NaeI GCCGGC 1 cut(s) 625
NarI GGCGCC 1 cut(s) 281
NciI CCSGG 1 cut(s) 466
NdeII GATC 5 cut(s) 51, 128, 370, 491, 505
NgoMIV GCCGGC 1 cut(s) 623
NlaIII CATG 4 cut(s) 86, 202, 232, 571
NlaIV GGNNCC 3 cut(s) 282, 527, 659
NmuCI GTSAC 2 cut(s) 223, 683
NspI RCATGY 2 cut(s) 202, 571
PaeI GCATGC 2 cut(s) 202, 571
PaeR7I CTCGAG 1 cut(s) 27
PdiI GCCGGC 1 cut(s) 625
PfeI GAWTC 1 cut(s) 208
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 6 cut(s) 197, 433, 530, 566, 692, 695
PluTI GGCGCC 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 373
PspEI GGTNACC 1 cut(s) 683
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 3 cut(s) 282, 527, 659
PspPI GGNCC 1 cut(s) 64
PspXI VCTCGAGB 1 cut(s) 27
PstNI CAGNNNCTG 2 cut(s) 124, 374
PsuI RGATCY 2 cut(s) 370, 505
RsaI GTAC 4 cut(s) 96, 251, 338, 701
RsaNI GTAC 4 cut(s) 95, 250, 337, 700
SatI GCNGC 6 cut(s) 196, 432, 529, 565, 691, 694
Sau3AI GATC 5 cut(s) 51, 128, 370, 491, 505
Sau96I GGNCC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 375, 466
SetI ASST 8 cut(s) 154, 225, 273, 300, 311, 460, 685, 698
SfaNI GCATC 1 cut(s) 625
SfoI GGCGCC 1 cut(s) 282
Sfr274I CTCGAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 64
SlaI CTCGAG 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 27, 618
SmoI CTYRAG 2 cut(s) 27, 618
SphI GCATGC 2 cut(s) 202, 571
Sse9I AATT 1 cut(s) 289
SsiI CCGC 8 cut(s) 164, 195, 431, 529, 588, 627, 668, 688
SspDI GGCGCC 1 cut(s) 280
SspMI CTAG 1 cut(s) 727
StyD4I CCNGG 2 cut(s) 373, 464
TaaI ACNGT 4 cut(s) 40, 259, 349, 675
TaiI ACGT 2 cut(s) 154, 225
TaqI TCGA 3 cut(s) 28, 50, 176
TasI AATT 1 cut(s) 289
TatI WGTACW 1 cut(s) 699
TauI GCSGC 3 cut(s) 198, 434, 531
TfiI GAWTC 1 cut(s) 208
TscAI CASTG 2 cut(s) 43, 566
TseFI GTSAC 2 cut(s) 223, 683
TseI GCWGC 3 cut(s) 564, 690, 693
Tsp45I GTSAC 2 cut(s) 223, 683
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 2 cut(s) 43, 566
Van91I CCANNNNNTGG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 1 cut(s) 289
XceI RCATGY 2 cut(s) 202, 571
XhoI CTCGAG 1 cut(s) 27
XspI CTAG 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.