Rmu_sc0000366.1_g000004

histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000366.1
OG0031065
Physical Location & Seq
Forward (+)
34671 .. 38983
4313 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 999 bp
atgcaaaaatccaaactgtactcgaggtatcggttcggtcttagtttcttcggaccggcccgacccgagtccagagtaaatttacaaaagcgcgccttcattgccctgcccgcgaaacccaaaagtccatttcaaaatcaaataagcattattagggccccgctcaatcacagccatccactacaaccagtcaaacatccaagtctccacgtcatcgatccgcacctgtctgtctccaccaatcaaatcccaccccgctctccgaaaccttgtcagctagcttctcctcgaccggtttcttcccggccggcttcttctctgccattgttgagggtttcagagctttctaggaagctccgagcggagaagaagcttccggcttcgaaggacgccgccgagaagaagaagaagaagaagaagaagcgtccaaagaagagcgtggagacctacaagacctacatctttaaggccatggggattatgaacagtttcatcaatgacatatttgagaagctggctgccgactcttccaagcttgcgaggtacaataagcagatgacgatcacttcgagggagattcaaactgcggtgaggttggtgcttcctggtctccggaggtggccggaaaaggtcgccggatttagtggcagagccgcgccgacttccgccaatggtgtcggggccgggctgctcctcttcatctcatcaagcttaacaactttcttaactagcataaactcaaggcgatctccccagttcaagaatcactcaaaacgaagctctacagctcgagatatccggatcgaaagttccgttcgatctaagtcggggttgacttgcagccgccgccacagaccactccaccgttcaaggctaccacaggaagctgcgcaaggaagaggcgaaggcgtgggacatgttctggggtcaattgatgtcctgtggagcgagaaccagcttggagaaggaatgctctgttttggttgctggtttcggtag

Protein Analysis

332

Amino Acids

37.14

Weight (kDa)

11.25

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 156 - 199 1.7e-09 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031065)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000366.1_g000004 Rmu_sc0000366.1_g000026

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 891
AccBSI CCGCTC 3 cut(s) 163, 258, 362
AccII CGCG 3 cut(s) 93, 113, 656
AccIII TCCGGA 2 cut(s) 612, 798
AclWI GGATC 2 cut(s) 212, 809
AcoI YGGCCR 2 cut(s) 305, 620
AcsI RAATTY 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 2 cut(s) 20, 545
AflIII ACRYGT 1 cut(s) 916
AgeI ACCGGT 1 cut(s) 292
AgsI TTSAA 4 cut(s) 134, 581, 760, 870
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 1 cut(s) 604
AloI GAACNNNNNNTCC 2 cut(s) 793, 825
Alw26I GTCTC 4 cut(s) 209, 238, 437, 614
AlwI GGATC 2 cut(s) 212, 809
Ama87I CYCGRG 3 cut(s) 22, 65, 789
Aor13HI TCCGGA 2 cut(s) 612, 798
AoxI GGCC 6 cut(s) 57, 156, 305, 468, 620, 681
ApaI GGGCCC 1 cut(s) 160
ApeKI GCWGC 4 cut(s) 518, 688, 840, 887
ApoI RAATTY 1 cut(s) 79
ArsI GACNNNNNNTTYG 2 cut(s) 550, 582
AsiGI ACCGGT 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 4 cut(s) 93, 95, 658, 892
AspS9I GGNCC 5 cut(s) 53, 58, 156, 157, 681
AsuC2I CCSGG 2 cut(s) 304, 685
AsuHPI GGTGA 1 cut(s) 601
AsuII TTCGAA 1 cut(s) 383
AsuNHI GCTAGC 1 cut(s) 277
AvaI CYCGRG 3 cut(s) 22, 65, 789
AvaII GGWCC 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 160
BbvI GCAGC 4 cut(s) 505, 675, 852, 874
BccI CCATC 1 cut(s) 183
BciT130I CCWGG 1 cut(s) 606
BcnI CCSGG 2 cut(s) 304, 685
BcoDI GTCTC 4 cut(s) 209, 238, 437, 614
BfaI CTAG 3 cut(s) 278, 348, 729
BfmI CTRYAG 1 cut(s) 783
BisI GCNGC 8 cut(s) 393, 519, 654, 689, 841, 844, 847, 888
BlsI GCNGC 8 cut(s) 394, 520, 655, 690, 842, 845, 848, 889
Bme1390I CCNGG 3 cut(s) 304, 606, 685
Bme18I GGWCC 1 cut(s) 53
BmeT110I CYCGRG 3 cut(s) 22, 65, 789
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 5 cut(s) 53, 58, 156, 157, 681
BmiI GGNNCC 3 cut(s) 158, 159, 682
BmrFI CCNGG 3 cut(s) 304, 606, 685
BmrI ACTGGG 1 cut(s) 748
BmtI GCTAGC 1 cut(s) 281
BmuI ACTGGG 1 cut(s) 748
BoxI GACNNNNGTC 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 724
BpuMI CCSGG 2 cut(s) 304, 685
Bsa29I ATCGAT 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 560
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 2 cut(s) 437, 614
BsaJI CCNNGG 1 cut(s) 471
BsaWI WCCGGW 3 cut(s) 292, 612, 798
Bse118I RCCGGY 3 cut(s) 55, 292, 307
Bse1I ACTGG 2 cut(s) 188, 754
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 560
BseAI TCCGGA 2 cut(s) 612, 798
BseBI CCWGG 1 cut(s) 606
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 2 cut(s) 175, 196
BseJI GATNNNNATC 1 cut(s) 560
BseMI GCAATG 1 cut(s) 99
BseNI ACTGG 2 cut(s) 188, 754
BsePI GCGCGC 1 cut(s) 91
BseRI GAGGAG 2 cut(s) 276, 683
BseSI GKGCMC 1 cut(s) 160
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 4 cut(s) 505, 675, 852, 874
Bsh1236I CGCG 3 cut(s) 93, 113, 656
Bsh1285I CGRYCG 2 cut(s) 293, 308
BshFI GGCC 6 cut(s) 59, 158, 307, 470, 622, 683
BshTI ACCGGT 1 cut(s) 292
BshVI ATCGAT 1 cut(s) 216
BsiEI CGRYCG 2 cut(s) 293, 308
BsiHKCI CYCGRG 3 cut(s) 22, 65, 789
BslFI GGGAC 1 cut(s) 927
BsmAI GTCTC 4 cut(s) 209, 238, 437, 614
BsmFI GGGAC 1 cut(s) 927
BsmI GAATGC 1 cut(s) 975
BsnI GGCC 6 cut(s) 59, 158, 307, 470, 622, 683
Bso31I GGTCTC 2 cut(s) 437, 614
BsoBI CYCGRG 3 cut(s) 22, 65, 789
Bsp119I TTCGAA 1 cut(s) 383
Bsp120I GGGCCC 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 160
Bsp13I TCCGGA 2 cut(s) 612, 798
Bsp143I GATC 5 cut(s) 217, 561, 746, 801, 818
Bsp19I CCATGG 1 cut(s) 471
BspANI GGCC 6 cut(s) 59, 158, 307, 470, 622, 683
BspDI ATCGAT 1 cut(s) 216
BspEI TCCGGA 2 cut(s) 612, 798
BspFNI CGCG 3 cut(s) 93, 113, 656
BspLI GGNNCC 3 cut(s) 158, 159, 682
BspOI GCTAGC 1 cut(s) 281
BspPI GGATC 2 cut(s) 212, 809
BspQI GCTCTTC 1 cut(s) 428
BspT104I TTCGAA 1 cut(s) 383
BspTNI GGTCTC 2 cut(s) 437, 614
BsrBI CCGCTC 3 cut(s) 163, 258, 362
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 3 cut(s) 55, 292, 307
BsrI ACTGG 2 cut(s) 188, 754
BssAI RCCGGY 3 cut(s) 55, 292, 307
BssECI CCNNGG 1 cut(s) 471
BssHII GCGCGC 1 cut(s) 91
BssMI GATC 5 cut(s) 217, 561, 746, 801, 818
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 3 cut(s) 18, 488, 866
Bst6I CTCTTC 4 cut(s) 428, 532, 701, 892
BstACI GRCGYC 1 cut(s) 390
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 6 cut(s) 93, 111, 279, 309, 516, 537
BstDEI CTNAG 2 cut(s) 41, 822
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 2 cut(s) 175, 196
BstFNI CGCG 3 cut(s) 93, 113, 656
BstHHI GCGC 4 cut(s) 93, 95, 658, 892
BstKTI GATC 5 cut(s) 220, 564, 749, 804, 821
BstMAI GTCTC 4 cut(s) 209, 238, 437, 614
BstMBI GATC 5 cut(s) 217, 561, 746, 801, 818
BstMCI CGRYCG 2 cut(s) 293, 308
BstMWI GCNNNNNNNGC 3 cut(s) 101, 110, 846
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 1 cut(s) 920
BstPAI GACNNNNGTC 1 cut(s) 67
BstSCI CCNGG 3 cut(s) 302, 604, 683
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 160
BstUI CGCG 3 cut(s) 93, 113, 656
BstV1I GCAGC 4 cut(s) 505, 675, 852, 874
BstZI CGGCCG 1 cut(s) 305
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 6 cut(s) 59, 158, 307, 470, 622, 683
BsuTUI ATCGAT 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 471
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 175, 196
Cac8I GCNNGC 6 cut(s) 93, 111, 279, 309, 516, 537
CfoI GCGC 4 cut(s) 93, 95, 658, 892
Cfr10I RCCGGY 3 cut(s) 55, 292, 307
Cfr13I GGNCC 5 cut(s) 53, 58, 156, 157, 681
ClaI ATCGAT 1 cut(s) 216
CpoI CGGWCCG 1 cut(s) 53
CseI GACGC 2 cut(s) 398, 413
Csp6I GTAC 2 cut(s) 19, 544
CspAI ACCGGT 1 cut(s) 292
CspI CGGWCCG 1 cut(s) 53
CviAII CATG 2 cut(s) 472, 917
CviQI GTAC 2 cut(s) 19, 544
DdeI CTNAG 2 cut(s) 41, 822
DpnI GATC 5 cut(s) 219, 563, 748, 803, 820
DpnII GATC 5 cut(s) 217, 561, 746, 801, 818
EaeI YGGCCR 2 cut(s) 305, 620
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 4 cut(s) 428, 532, 701, 892
EarI CTCTTC 4 cut(s) 428, 532, 701, 892
EciI GGCGGA 1 cut(s) 655
EclXI CGGCCG 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 160
Eco31I GGTCTC 2 cut(s) 437, 614
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 305
Eco88I CYCGRG 3 cut(s) 22, 65, 789
EcoO109I RGGNCCY 2 cut(s) 156, 157
EcoRII CCWGG 1 cut(s) 604
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 2 cut(s) 475, 920
FaiI YATR 5 cut(s) 473, 482, 503, 734, 918
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 1 cut(s) 927
FatI CATG 2 cut(s) 471, 916
FauI CCCGC 3 cut(s) 118, 168, 263
Fnu4HI GCNGC 8 cut(s) 393, 519, 654, 689, 841, 844, 847, 888
FokI GGATG 2 cut(s) 162, 183
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 8 cut(s) 393, 519, 654, 689, 841, 844, 847, 888
FspBI CTAG 3 cut(s) 278, 348, 729
FspI TGCGCA 1 cut(s) 891
GlaI GCGC 4 cut(s) 92, 94, 657, 891
GluI GCNGC 8 cut(s) 393, 519, 654, 689, 841, 844, 847, 888
HaeIII GGCC 6 cut(s) 59, 158, 307, 470, 622, 683
HgaI GACGC 2 cut(s) 398, 413
HhaI GCGC 4 cut(s) 93, 95, 658, 892
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 2 cut(s) 475, 920
Hin6I GCGC 4 cut(s) 91, 93, 656, 890
HinP1I GCGC 4 cut(s) 91, 93, 656, 890
HincII GTYRAC 1 cut(s) 834
HindII GTYRAC 1 cut(s) 834
HindIII AAGCTT 3 cut(s) 371, 533, 709
HinfI GANTC 4 cut(s) 68, 524, 577, 763
HphI GGTGA 1 cut(s) 601
Hpy166II GTNNAC 1 cut(s) 834
Hpy188I TCNGA 4 cut(s) 53, 264, 340, 359
Hpy188III TCNNGA 5 cut(s) 72, 613, 760, 791, 799
Hpy8I GTNNAC 1 cut(s) 834
HpyAV CCTTC 4 cut(s) 106, 379, 899, 959
HpyCH4III ACNGT 3 cut(s) 18, 488, 866
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 4, 840
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 110, 846
HpyF3I CTNAG 2 cut(s) 41, 822
HpySE526I ACGT 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 2 cut(s) 475, 920
HspAI GCGC 4 cut(s) 91, 93, 656, 890
Kpn2I TCCGGA 2 cut(s) 612, 798
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Kzo9I GATC 5 cut(s) 217, 561, 746, 801, 818
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 3 cut(s) 360, 696, 945
Lsp1109I GCAGC 4 cut(s) 505, 675, 852, 874
MaeI CTAG 3 cut(s) 278, 348, 729
MaeII ACGT 1 cut(s) 210
MalI GATC 5 cut(s) 219, 563, 748, 803, 820
MbiI CCGCTC 3 cut(s) 163, 258, 362
MboI GATC 5 cut(s) 217, 561, 746, 801, 818
MfeI CAATTG 1 cut(s) 930
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 2 cut(s) 79, 930
MlyI GAGTC 2 cut(s) 77, 518
MnlI CCTC 9 cut(s) 18, 297, 324, 534, 564, 585, 609, 704, 893
MroI TCCGGA 2 cut(s) 612, 798
MroNI GCCGGC 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 3 cut(s) 465, 713, 725
MspR9I CCNGG 3 cut(s) 304, 606, 685
MunI CAATTG 1 cut(s) 930
Mva1269I GAATGC 1 cut(s) 975
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 3 cut(s) 93, 113, 656
MwoI GCNNNNNNNGC 3 cut(s) 101, 110, 846
NaeI GCCGGC 1 cut(s) 309
NciI CCSGG 2 cut(s) 304, 685
NcoI CCATGG 1 cut(s) 471
NdeII GATC 5 cut(s) 217, 561, 746, 801, 818
NgoMIV GCCGGC 1 cut(s) 307
NheI GCTAGC 1 cut(s) 277
NlaIII CATG 2 cut(s) 475, 920
NlaIV GGNNCC 3 cut(s) 158, 159, 682
NmeAIII GCCGAG 1 cut(s) 421
NsbI TGCGCA 1 cut(s) 891
NspI RCATGY 1 cut(s) 920
NspV TTCGAA 1 cut(s) 383
PaeR7I CTCGAG 2 cut(s) 22, 789
PauI GCGCGC 1 cut(s) 91
PciI ACATGT 1 cut(s) 916
PciSI GCTCTTC 1 cut(s) 428
PcsI WCGNNNNNNNCGW 2 cut(s) 566, 810
PctI GAATGC 1 cut(s) 975
PdiI GCCGGC 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 2 cut(s) 577, 763
PinAI ACCGGT 1 cut(s) 292
PkrI GCNGC 8 cut(s) 394, 520, 655, 690, 842, 845, 848, 889
PleI GAGTC 2 cut(s) 76, 518
PpsI GAGTC 2 cut(s) 76, 518
PscI ACATGT 1 cut(s) 916
PshAI GACNNNNGTC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 3 cut(s) 158, 159, 682
PspOMI GGGCCC 1 cut(s) 156
PspPI GGNCC 5 cut(s) 53, 58, 156, 157, 681
PspXI VCTCGAGB 1 cut(s) 22
PteI GCGCGC 1 cut(s) 91
RsaI GTAC 2 cut(s) 20, 545
RsaNI GTAC 2 cut(s) 19, 544
Rsr2I CGGWCCG 1 cut(s) 53
RsrII CGGWCCG 1 cut(s) 53
SapI GCTCTTC 1 cut(s) 428
SaqAI TTAA 3 cut(s) 465, 713, 725
SatI GCNGC 8 cut(s) 393, 519, 654, 689, 841, 844, 847, 888
Sau3AI GATC 5 cut(s) 217, 561, 746, 801, 818
Sau96I GGNCC 5 cut(s) 53, 58, 156, 157, 681
SchI GAGTC 2 cut(s) 77, 518
ScrFI CCNGG 3 cut(s) 304, 606, 685
SduI GDGCHC 1 cut(s) 160
SfcI CTRYAG 1 cut(s) 783
Sfr274I CTCGAG 2 cut(s) 22, 789
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 1 cut(s) 53
SlaI CTCGAG 2 cut(s) 22, 789
SmlI CTYRAG 3 cut(s) 22, 739, 789
SmoI CTYRAG 3 cut(s) 22, 739, 789
Sse9I AATT 2 cut(s) 79, 930
SspMI CTAG 3 cut(s) 278, 348, 729
StyD4I CCNGG 3 cut(s) 302, 604, 683
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 3 cut(s) 18, 488, 866
TaiI ACGT 1 cut(s) 213
TaqI TCGA 8 cut(s) 23, 216, 289, 383, 569, 790, 804, 817
TaqII GACCGA 1 cut(s) 26
TasI AATT 2 cut(s) 79, 930
TatI WGTACW 1 cut(s) 18
TauI GCSGC 4 cut(s) 395, 656, 846, 849
TfiI GAWTC 2 cut(s) 577, 763
Tru1I TTAA 3 cut(s) 465, 713, 725
Tru9I TTAA 3 cut(s) 465, 713, 725
TseI GCWGC 4 cut(s) 518, 688, 840, 887
TspDTI ATGAA 4 cut(s) 88, 481, 497, 688
TspGWI ACGGA 1 cut(s) 802
VpaK11BI GGWCC 1 cut(s) 53
XapI RAATTY 1 cut(s) 79
XceI RCATGY 1 cut(s) 920
XhoI CTCGAG 2 cut(s) 22, 789
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 3 cut(s) 278, 348, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.