Rmu_sc0000474.1_g000004

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000474.1
OG0031072
Physical Location & Seq
Reverse (-)
29392 .. 36375
6984 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1707 bp
atggtccgggttcagttcttggcggttcttgactgcgtggaccggaaaaccgagcttatgtacgtgtcgtccaattataagtttgttttcgaaccctcagtcactcactcactcagcccccagaaatcggtattcctcacctctatcagtctctcctcacaatcgatcttcctcacaatcgatctggactgctcgatcttctctgaaatcgatcttcctcacaatcgttcttcctcagccctcagtcactcactcactcagcccccagaaatcggtattcctcacctctggtcttctcctcgacgtcacctccgacggtccggcctctcattcttagtctcttcagtctctctatctcattctttgccttttttctgcagggaagagaaagatcctgctgcacagagaaagagagagagaaagatctcgccgttgtccgatatgcctccgagcaagcctgggccaacgccggtgctgagggaaagcattacccccagaactgttttgatcattctggctggaaggttcaagggcaagagggttatctttttgaagcagctgtgttctggcttgcttttggtcactgtcagttctcattatgatggtgacaatgatgaaatggttgttgttgggaatgatggaaatgatgtgtctcagcaaggtgaggacaaggaaccagccagggaaatagaagctacattacaaacagaaaatgaagcaacaactcaagcatcccgaggttggcaaagagccctagctgaatcagacgaagtggttgttgagagtgaagttgataggtatctattagatccaatggagaatcccaaatatgaagattggcagttgcttcaatggctaacatctccatggccgccaaccgcgcagccgtatcttctccgccgcagcgaagaaggaccatgtccgctctcgaagcccgaatctccctcgtcactgctctcgccacccaagcctcctccgtctcctagcaacataaatgttcaaatccctttcccacaatctaaaattttccttggttccgatttagagagagaaaacgcgcgcggacaaattagaccaggaacgaatggtgagtatgatttggtacatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgatgactgtgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

568

Amino Acids

64.18

Weight (kDa)

4.05

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0031072)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000474.1_g000004 Rmu_sc0004339.1_g000022

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 78
AatII GACGTC 1 cut(s) 307
AccBSI CCGCTC 1 cut(s) 925
AccII CGCG 4 cut(s) 881, 1058, 1060, 1062
AciI CCGC 7 cut(s) 23, 872, 879, 898, 901, 923, 1062
AclWI GGATC 2 cut(s) 386, 803
AcoI YGGCCR 1 cut(s) 869
AcsI RAATTY 1 cut(s) 1023
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 1 cut(s) 304
AfaI GTAC 2 cut(s) 62, 1104
AfiI CCNNNNNNNGG 3 cut(s) 127, 272, 741
AflIII ACRYGT 1 cut(s) 63
AgsI TTSAA 4 cut(s) 529, 553, 851, 1001
AjnI CCWGG 3 cut(s) 457, 680, 1075
AluBI AGCT 4 cut(s) 55, 559, 695, 758
AluI AGCT 4 cut(s) 55, 559, 695, 758
Alw26I GTCTC 5 cut(s) 155, 343, 352, 657, 984
AlwI GGATC 2 cut(s) 386, 803
Ama87I CYCGRG 1 cut(s) 735
AoxI GGCC 3 cut(s) 322, 461, 869
ApeKI GCWGC 4 cut(s) 398, 556, 883, 903
ApoI RAATTY 1 cut(s) 1023
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AspLEI GCGC 3 cut(s) 883, 1060, 1062
AspS9I GGNCC 5 cut(s) 4, 40, 318, 461, 914
AsuC2I CCSGG 1 cut(s) 8
AsuHPI GGTGA 6 cut(s) 130, 275, 299, 617, 674, 1100
AsuII TTCGAA 1 cut(s) 90
AvaI CYCGRG 1 cut(s) 735
AvaII GGWCC 4 cut(s) 4, 40, 318, 914
BanII GRGCYC 1 cut(s) 754
BarI GAAGNNNNNNTAC 2 cut(s) 684, 716
BbsI GAAGAC 1 cut(s) 285
BbvCI CCTCAGC 2 cut(s) 235, 476
BbvI GCAGC 4 cut(s) 385, 568, 895, 915
BccI CCATC 2 cut(s) 596, 632
BceAI ACGGC 2 cut(s) 415, 871
BciT130I CCWGG 3 cut(s) 459, 682, 1077
BclI TGATCA 1 cut(s) 507
BcnI CCSGG 1 cut(s) 8
BcoDI GTCTC 5 cut(s) 155, 343, 352, 657, 984
BfaI CTAG 2 cut(s) 755, 984
BfmI CTRYAG 1 cut(s) 376
BglII AGATCT 1 cut(s) 423
BisI GCNGC 6 cut(s) 399, 557, 872, 884, 901, 904
BlsI GCNGC 6 cut(s) 400, 558, 873, 885, 902, 905
Bme1390I CCNGG 4 cut(s) 8, 459, 682, 1077
Bme18I GGWCC 4 cut(s) 4, 40, 318, 914
BmeT110I CYCGRG 1 cut(s) 735
BmgT120I GGNCC 5 cut(s) 4, 40, 318, 461, 914
BmiI GGNNCC 2 cut(s) 675, 1036
BmrFI CCNGG 4 cut(s) 8, 459, 682, 1077
BmsI GCATC 1 cut(s) 740
BpiI GAAGAC 1 cut(s) 285
Bpu10I CCTNAGC 2 cut(s) 235, 476
Bpu14I TTCGAA 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 711
BpuMI CCSGG 1 cut(s) 8
Bsa29I ATCGAT 3 cut(s) 164, 180, 210
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 1 cut(s) 304
BsaJI CCNNGG 5 cut(s) 458, 681, 736, 866, 1030
BsaWI WCCGGW 1 cut(s) 42
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 272, 741
Bse118I RCCGGY 1 cut(s) 469
Bse8I GATNNNNATC 1 cut(s) 798
BseBI CCWGG 3 cut(s) 459, 682, 1077
BseCI ATCGAT 3 cut(s) 164, 180, 210
BseDI CCNNGG 5 cut(s) 458, 681, 736, 866, 1030
BseGI GGATG 1 cut(s) 731
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 3 cut(s) 127, 272, 741
BseMII CTCAG 7 cut(s) 111, 127, 249, 256, 272, 467, 668
BsePI GCGCGC 1 cut(s) 1058
BseRI GAGGAG 3 cut(s) 145, 288, 963
BseXI GCAGC 4 cut(s) 385, 568, 895, 915
BsgI GTGCAG 1 cut(s) 384
Bsh1236I CGCG 4 cut(s) 881, 1058, 1060, 1062
BshFI GGCC 3 cut(s) 324, 463, 871
BshVI ATCGAT 3 cut(s) 164, 180, 210
BsiHKCI CYCGRG 1 cut(s) 735
BsiSI CCGG 4 cut(s) 7, 43, 321, 470
BslI CCNNNNNNNGG 3 cut(s) 127, 272, 741
BsmAI GTCTC 5 cut(s) 155, 343, 352, 657, 984
BsmBI CGTCTC 1 cut(s) 984
BsnI GGCC 3 cut(s) 324, 463, 871
BsoBI CYCGRG 1 cut(s) 735
Bsp119I TTCGAA 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 754
Bsp143I GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 7 cut(s) 23, 872, 879, 898, 901, 923, 1062
BspANI GGCC 3 cut(s) 324, 463, 871
BspCNI CTCAG 7 cut(s) 110, 126, 248, 255, 271, 468, 667
BspDI ATCGAT 3 cut(s) 164, 180, 210
BspFNI CGCG 4 cut(s) 881, 1058, 1060, 1062
BspLI GGNNCC 2 cut(s) 675, 1036
BspMAI CTGCAG 1 cut(s) 380
BspPI GGATC 2 cut(s) 386, 803
BspT104I TTCGAA 1 cut(s) 90
BsrBI CCGCTC 1 cut(s) 925
BsrFI RCCGGY 1 cut(s) 469
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 5 cut(s) 458, 681, 736, 866, 1030
BssHII GCGCGC 1 cut(s) 1058
BssMI GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
BssNI GRCGYC 1 cut(s) 304
BssT1I CCWWGG 2 cut(s) 866, 1030
Bst2UI CCWGG 3 cut(s) 459, 682, 1077
Bst4CI ACNGT 4 cut(s) 318, 502, 586, 1700
Bst6I CTCTTC 2 cut(s) 346, 378
BstACI GRCGYC 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 455, 572, 1060
BstDEI CTNAG 8 cut(s) 97, 113, 235, 242, 258, 334, 476, 654
BstDSI CCRYGG 1 cut(s) 866
BstF5I GGATG 1 cut(s) 731
BstFNI CGCG 4 cut(s) 881, 1058, 1060, 1062
BstHHI GCGC 3 cut(s) 883, 1060, 1062
BstKTI GATC 8 cut(s) 168, 184, 198, 214, 394, 426, 510, 811
BstMAI GTCTC 5 cut(s) 155, 343, 352, 657, 984
BstMBI GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
BstMWI GCNNNNNNNGC 4 cut(s) 853, 880, 931, 967
BstNI CCWGG 3 cut(s) 459, 682, 1077
BstSCI CCNGG 4 cut(s) 6, 457, 680, 1075
BstSFI CTRYAG 1 cut(s) 376
BstUI CGCG 4 cut(s) 881, 1058, 1060, 1062
BstV1I GCAGC 4 cut(s) 385, 568, 895, 915
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 3 cut(s) 391, 423, 808
BstYI RGATCY 3 cut(s) 391, 423, 808
Bsu15I ATCGAT 3 cut(s) 164, 180, 210
BsuRI GGCC 3 cut(s) 324, 463, 871
BsuTUI ATCGAT 3 cut(s) 164, 180, 210
BtgI CCRYGG 1 cut(s) 866
BtsCI GGATG 1 cut(s) 731
BtsI GCAGTG 1 cut(s) 950
BtsIMutI CAGTG 2 cut(s) 582, 950
Cac8I GCNNGC 3 cut(s) 455, 572, 1060
CfoI GCGC 3 cut(s) 883, 1060, 1062
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 5 cut(s) 4, 40, 318, 461, 914
ClaI ATCGAT 3 cut(s) 164, 180, 210
CpoI CGGWCCG 1 cut(s) 318
Csp6I GTAC 2 cut(s) 61, 1103
CspI CGGWCCG 1 cut(s) 318
CviAII CATG 3 cut(s) 867, 918, 1106
CviQI GTAC 2 cut(s) 61, 1103
DdeI CTNAG 8 cut(s) 97, 113, 235, 242, 258, 334, 476, 654
DpnI GATC 8 cut(s) 167, 183, 197, 213, 393, 425, 509, 810
DpnII GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
EaeI YGGCCR 1 cut(s) 869
Eam1104I CTCTTC 2 cut(s) 346, 378
EarI CTCTTC 2 cut(s) 346, 378
EciI GGCGGA 1 cut(s) 887
Eco130I CCWWGG 2 cut(s) 866, 1030
Eco24I GRGCYC 1 cut(s) 754
Eco47I GGWCC 4 cut(s) 4, 40, 318, 914
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 735
EcoRII CCWGG 3 cut(s) 457, 680, 1075
EcoT14I CCWWGG 2 cut(s) 866, 1030
EcoT38I GRGCYC 1 cut(s) 754
ErhI CCWWGG 2 cut(s) 866, 1030
Esp3I CGTCTC 1 cut(s) 984
FaeI CATG 3 cut(s) 870, 921, 1109
FatI CATG 3 cut(s) 866, 917, 1105
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 6 cut(s) 399, 557, 872, 884, 901, 904
FokI GGATG 1 cut(s) 718
FriOI GRGCYC 1 cut(s) 754
Fsp4HI GCNGC 6 cut(s) 399, 557, 872, 884, 901, 904
FspBI CTAG 2 cut(s) 755, 984
GlaI GCGC 3 cut(s) 882, 1059, 1061
GluI GCNGC 6 cut(s) 399, 557, 872, 884, 901, 904
HaeIII GGCC 3 cut(s) 324, 463, 871
HapII CCGG 4 cut(s) 7, 43, 321, 470
HhaI GCGC 3 cut(s) 883, 1060, 1062
Hin1I GRCGYC 1 cut(s) 304
Hin1II CATG 3 cut(s) 870, 921, 1109
Hin6I GCGC 3 cut(s) 881, 1058, 1060
HinP1I GCGC 3 cut(s) 881, 1058, 1060
HinfI GANTC 3 cut(s) 761, 820, 938
HpaII CCGG 4 cut(s) 7, 43, 321, 470
HphI GGTGA 6 cut(s) 130, 275, 299, 617, 674, 1100
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 6 cut(s) 205, 314, 439, 450, 766, 1039
Hpy188III TCNNGA 4 cut(s) 29, 185, 735, 928
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 2 cut(s) 306, 318
HpyAV CCTTC 2 cut(s) 516, 905
HpyCH4III ACNGT 4 cut(s) 318, 502, 586, 1700
HpyCH4IV ACGT 2 cut(s) 63, 304
HpyCH4V TGCA 2 cut(s) 378, 401
HpyF10VI GCNNNNNNNGC 4 cut(s) 853, 880, 931, 967
HpyF3I CTNAG 8 cut(s) 97, 113, 235, 242, 258, 334, 476, 654
HpySE526I ACGT 2 cut(s) 63, 304
Hsp92I GRCGYC 1 cut(s) 304
Hsp92II CATG 3 cut(s) 870, 921, 1109
HspAI GCGC 3 cut(s) 881, 1058, 1060
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
Lsp1109I GCAGC 4 cut(s) 385, 568, 895, 915
LweI GCATC 1 cut(s) 740
MaeI CTAG 2 cut(s) 755, 984
MaeII ACGT 2 cut(s) 63, 304
MaeIII GTNAC 6 cut(s) 100, 245, 305, 580, 605, 948
MalI GATC 8 cut(s) 167, 183, 197, 213, 393, 425, 509, 810
MauBI CGCGCGCG 1 cut(s) 1058
MbiI CCGCTC 1 cut(s) 925
MboI GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
MflI RGATCY 3 cut(s) 391, 423, 808
MhlI GDGCHC 1 cut(s) 754
MluCI AATT 3 cut(s) 73, 1023, 1068
MmeI TCCRAC 1 cut(s) 337
MslI CAYNNNNRTG 3 cut(s) 600, 865, 1110
MspA1I CMGCKG 1 cut(s) 559
MspI CCGG 4 cut(s) 7, 43, 321, 470
MspR9I CCNGG 4 cut(s) 8, 459, 682, 1077
MvaI CCWGG 3 cut(s) 459, 682, 1077
MvnI CGCG 4 cut(s) 881, 1058, 1060, 1062
MwoI GCNNNNNNNGC 4 cut(s) 853, 880, 931, 967
NciI CCSGG 1 cut(s) 8
NcoI CCATGG 1 cut(s) 866
NdeII GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
NlaIII CATG 3 cut(s) 870, 921, 1109
NlaIV GGNNCC 2 cut(s) 675, 1036
NmuCI GTSAC 6 cut(s) 100, 245, 305, 580, 605, 948
NspV TTCGAA 1 cut(s) 90
PauI GCGCGC 1 cut(s) 1058
PcsI WCGNNNNNNNCGW 1 cut(s) 310
PfeI GAWTC 3 cut(s) 761, 820, 938
PflFI GACNNNGTC 1 cut(s) 918
PkrI GCNGC 6 cut(s) 400, 558, 873, 885, 902, 905
Ppu21I YACGTR 1 cut(s) 64
PsiI TTATAA 1 cut(s) 78
Psp6I CCWGG 3 cut(s) 457, 680, 1075
PspGI CCWGG 3 cut(s) 457, 680, 1075
PspN4I GGNNCC 2 cut(s) 675, 1036
PspPI GGNCC 5 cut(s) 4, 40, 318, 461, 914
PstI CTGCAG 1 cut(s) 380
PsuI RGATCY 3 cut(s) 391, 423, 808
PsyI GACNNNGTC 1 cut(s) 918
PteI GCGCGC 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 2 cut(s) 62, 1104
RsaNI GTAC 2 cut(s) 61, 1103
RseI CAYNNNNRTG 3 cut(s) 600, 865, 1110
Rsr2I CGGWCCG 1 cut(s) 318
RsrII CGGWCCG 1 cut(s) 318
SatI GCNGC 6 cut(s) 399, 557, 872, 884, 901, 904
Sau3AI GATC 8 cut(s) 165, 181, 195, 211, 391, 423, 507, 808
Sau96I GGNCC 5 cut(s) 4, 40, 318, 461, 914
ScrFI CCNGG 4 cut(s) 8, 459, 682, 1077
SduI GDGCHC 1 cut(s) 754
SfaNI GCATC 1 cut(s) 740
SfcI CTRYAG 1 cut(s) 376
SfuI TTCGAA 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 469
SinI GGWCC 4 cut(s) 4, 40, 318, 914
SmiMI CAYNNNNRTG 3 cut(s) 600, 865, 1110
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
Sse9I AATT 3 cut(s) 73, 1023, 1068
SsiI CCGC 7 cut(s) 23, 872, 879, 898, 901, 923, 1062
SspMI CTAG 2 cut(s) 755, 984
StyD4I CCNGG 4 cut(s) 6, 457, 680, 1075
StyI CCWWGG 2 cut(s) 866, 1030
TaaI ACNGT 4 cut(s) 318, 502, 586, 1700
TaiI ACGT 2 cut(s) 66, 307
TaqI TCGA 7 cut(s) 90, 164, 180, 194, 210, 301, 929
TasI AATT 3 cut(s) 73, 1023, 1068
TauI GCSGC 2 cut(s) 874, 903
TfiI GAWTC 3 cut(s) 761, 820, 938
TscAI CASTG 2 cut(s) 589, 957
TseFI GTSAC 6 cut(s) 100, 245, 305, 580, 605, 948
TseI GCWGC 4 cut(s) 398, 556, 883, 903
Tsp45I GTSAC 6 cut(s) 100, 245, 305, 580, 605, 948
TspDTI ATGAA 3 cut(s) 630, 729, 846
TspGWI ACGGA 1 cut(s) 966
TspRI CASTG 2 cut(s) 589, 957
Tth111I GACNNNGTC 1 cut(s) 918
VpaK11BI GGWCC 4 cut(s) 4, 40, 318, 914
XapI RAATTY 1 cut(s) 1023
XspI CTAG 2 cut(s) 755, 984
ZraI GACGTC 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.