Rmu_sc0000516.1_g000023

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000516.1
OG0023046
Physical Location & Seq
Forward (+)
91392 .. 92000
609 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
atggtgatcgcattgattccaattctgaagagaaatgaaatagcagaacgaaaccctgttctcatagttttccctagttcagttatccacaactgggagagtgaattctccaagtggtctaactttagtgttgctgtttaccatggtgcaaatcgtgatttgatttatgataaattggaagctcatggagttgagatacttatcacaagctttgacacatacagaatttgtggcagcccgtttttagatgttaattgggagattgttgttgtcgatgaggcacaccggtttaagaatgaacaatcaaagctctacatagcatgtctagaatttaaaaccttaaaacgaattgcttttactggaactatgatgaagaacaaaatcatggaattgtttaacctgtttgactgggttgcacctggatcgttgggaacacgggaacattttcgggagttttatgatgaacctgtcaaacatggccaaagatcaactgcttcggaaggatttgtcttcattgctgatgagcgaaaacagcatctagtagctgttaatgttgagaaggacaaaagaggaaaccattggtcatcttatcatggggaaggaagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

202

Amino Acids

23.45

Weight (kDa)

6.51

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 2 - 165 5.5e-35 SNF2-related domain
DEAD PF00270 3 - 126 1e-06 DEAD/DEAH box helicase
ResIII PF04851 9 - 123 9.1e-06 Type III restriction enzyme, res subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023046)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000516.1_g000023 Rmu_sc0000516.1_g000063 Rmu_sc0000516.1_g000066 Rmu_sc0018229.1_g000004

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 1 cut(s) 478
AcsI RAATTY 3 cut(s) 104, 225, 329
AcuI CTGAAG 1 cut(s) 47
AfiI CCNNNNNNNGG 1 cut(s) 94
AgeI ACCGGT 1 cut(s) 285
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 4 cut(s) 182, 210, 310, 545
AluI AGCT 4 cut(s) 182, 210, 310, 545
AlwI GGATC 1 cut(s) 430
AoxI GGCC 1 cut(s) 478
ApeKI GCWGC 1 cut(s) 234
ApoI RAATTY 3 cut(s) 104, 225, 329
AsiGI ACCGGT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 444
AsuHPI GGTGA 1 cut(s) 16
BalI TGGCCA 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 246
BcgI CGANNNNNNTGC 2 cut(s) 405, 439
BciT130I CCWGG 1 cut(s) 420
BfaI CTAG 3 cut(s) 75, 326, 539
BisI GCNGC 1 cut(s) 235
BlsI GCNGC 1 cut(s) 236
Bme1390I CCNGG 1 cut(s) 420
BmrFI CCNGG 1 cut(s) 420
BmrI ACTGGG 2 cut(s) 103, 418
BmsI GCATC 1 cut(s) 544
BmuI ACTGGG 2 cut(s) 103, 418
BpiI GAAGAC 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 285
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 285
Bse1I ACTGG 3 cut(s) 98, 364, 413
Bse3DI GCAATG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 200
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 142
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 513
BseNI ACTGG 3 cut(s) 98, 364, 413
BseXI GCAGC 1 cut(s) 246
BshFI GGCC 1 cut(s) 480
BshTI ACCGGT 1 cut(s) 285
BsiSI CCGG 1 cut(s) 286
BslI CCNNNNNNNGG 1 cut(s) 94
BsnI GGCC 1 cut(s) 480
Bsp143I GATC 3 cut(s) 6, 422, 485
Bsp19I CCATGG 1 cut(s) 142
BspANI GGCC 1 cut(s) 480
BspPI GGATC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 513
BsrFI RCCGGY 1 cut(s) 285
BsrI ACTGG 3 cut(s) 98, 364, 413
BssAI RCCGGY 1 cut(s) 285
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 3 cut(s) 6, 422, 485
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 420
Bst6I CTCTTC 1 cut(s) 23
BstDSI CCRYGG 1 cut(s) 142
BstKTI GATC 3 cut(s) 9, 425, 488
BstMBI GATC 3 cut(s) 6, 422, 485
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 418
BstV1I GCAGC 1 cut(s) 246
BstV2I GAAGAC 1 cut(s) 502
BsuRI GGCC 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 285
CspAI ACCGGT 1 cut(s) 285
CviAII CATG 6 cut(s) 143, 185, 321, 385, 476, 593
CviJI RGCY 6 cut(s) 182, 210, 237, 310, 480, 545
CviKI_1 RGCY 6 cut(s) 182, 210, 237, 310, 480, 545
DpnI GATC 3 cut(s) 8, 424, 487
DpnII GATC 3 cut(s) 6, 422, 485
DraI TTTAAA 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 6 cut(s) 146, 188, 324, 388, 479, 596
FatI CATG 6 cut(s) 142, 184, 320, 384, 475, 592
Fnu4HI GCNGC 1 cut(s) 235
Fsp4HI GCNGC 1 cut(s) 235
FspBI CTAG 3 cut(s) 75, 326, 539
GluI GCNGC 1 cut(s) 235
HaeIII GGCC 1 cut(s) 480
HapII CCGG 1 cut(s) 286
Hin1II CATG 6 cut(s) 146, 188, 324, 388, 479, 596
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 1 cut(s) 16
HpaII CCGG 1 cut(s) 286
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 2 cut(s) 27, 499
Hpy188III TCNNGA 3 cut(s) 155, 326, 449
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 494, 553, 593
HpyCH4V TGCA 2 cut(s) 149, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
Hsp92II CATG 6 cut(s) 146, 188, 324, 388, 479, 596
Kzo9I GATC 3 cut(s) 6, 422, 485
LpnPI CCDG 9 cut(s) 69, 79, 299, 345, 394, 405, 413, 432, 480
Lsp1109I GCAGC 1 cut(s) 246
LweI GCATC 1 cut(s) 544
MaeI CTAG 3 cut(s) 75, 326, 539
MalI GATC 3 cut(s) 8, 424, 487
MboI GATC 3 cut(s) 6, 422, 485
MboII GAAGA 3 cut(s) 40, 385, 502
MlsI TGGCCA 1 cut(s) 480
MluCI AATT 8 cut(s) 21, 104, 173, 225, 253, 329, 348, 389
MluNI TGGCCA 1 cut(s) 480
MnlI CCTC 2 cut(s) 271, 563
Mox20I TGGCCA 1 cut(s) 480
MroXI GAANNNNTTC 1 cut(s) 444
MscI TGGCCA 1 cut(s) 480
MseI TTAA 6 cut(s) 252, 291, 333, 341, 396, 549
Msp20I TGGCCA 1 cut(s) 480
MspI CCGG 1 cut(s) 286
MspR9I CCNGG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 1 cut(s) 532
NcoI CCATGG 1 cut(s) 142
NdeII GATC 3 cut(s) 6, 422, 485
NlaIII CATG 6 cut(s) 146, 188, 324, 388, 479, 596
NspI RCATGY 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 1 cut(s) 16
PinAI ACCGGT 1 cut(s) 285
PkrI GCNGC 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
SaqAI TTAA 6 cut(s) 252, 291, 333, 341, 396, 549
SatI GCNGC 1 cut(s) 235
Sau3AI GATC 3 cut(s) 6, 422, 485
ScrFI CCNGG 1 cut(s) 420
SetI ASST 8 cut(s) 184, 212, 312, 341, 402, 421, 469, 547
SfaNI GCATC 1 cut(s) 544
Sse9I AATT 8 cut(s) 21, 104, 173, 225, 253, 329, 348, 389
SspMI CTAG 3 cut(s) 75, 326, 539
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 1 cut(s) 142
TaqI TCGA 1 cut(s) 273
TasI AATT 8 cut(s) 21, 104, 173, 225, 253, 329, 348, 389
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 6 cut(s) 252, 291, 333, 341, 396, 549
Tru9I TTAA 6 cut(s) 252, 291, 333, 341, 396, 549
TseI GCWGC 1 cut(s) 234
TspDTI ATGAA 5 cut(s) 51, 312, 386, 477, 502
XapI RAATTY 3 cut(s) 104, 225, 329
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 1 cut(s) 324
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 3 cut(s) 75, 326, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.