Rmu_sc0000516.1_g000063

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000516.1
OG0023046
Physical Location & Seq
Reverse (-)
268347 .. 270189
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
atggtgatcgcattgattccaattctgaagagaaataaaatagcagaacgaaaccctgttctcatagttttccctagttcagttatccacaactgggagagtgaattctctaagtggtctaactttagtgttgctgtttaccatggtgcaaatcgtgatttgatttatgataaattggaagctcatggagttgagatacttatcacaagctttaacacatacagaatttgtggcagccagttttcatatgttaattgggggattgttgttgtcgatgaggcacaccggtttaagaatgaaaaatcaaagctctacatagcatgtctagaatttaaaacctcaaaacgaattgctttcactggaactatgatgaagaacaaaatcatggaattgtttaacctgtttgactgggttgcacctggatcgttaggaacatgggaacattttcgggagttttatgatgaacctctcaaacatggccaaagatcaactgctccggaaggatttgtccgcattgctgatgagcgaaaacagcatctagtagctgttaatgttgagaaggacaaaagaggaaaccattggagcgtgaggatctcagccgcggcctcggattttccttctgaaatggctgatgcagcctccatcagcaactgctttgaacacctcatagtcgccggagatgccaccgaggaggatgaagacgacgatgaagtaggcgaaggcgaaaccggcttctgggtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

247

Amino Acids

28.17

Weight (kDa)

5.4

Isoelectric Point (pI)

26.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 2 - 162 8.6e-36 SNF2-related domain
DEAD PF00270 3 - 126 2.9e-07 DEAD/DEAH box helicase
ResIII PF04851 10 - 123 2.7e-06 Type III restriction enzyme, res subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023046)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000516.1_g000023 Rmu_sc0000516.1_g000063 Rmu_sc0000516.1_g000066 Rmu_sc0018229.1_g000004

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 602
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 3 cut(s) 511, 600, 602
AclWI GGATC 2 cut(s) 430, 599
AcoI YGGCCR 1 cut(s) 478
AcsI RAATTY 3 cut(s) 104, 225, 329
AcuI CTGAAG 1 cut(s) 47
AfiI CCNNNNNNNGG 2 cut(s) 94, 735
AgeI ACCGGT 1 cut(s) 285
AgsI TTSAA 1 cut(s) 659
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 4 cut(s) 182, 210, 310, 545
AluI AGCT 4 cut(s) 182, 210, 310, 545
AlwI GGATC 2 cut(s) 430, 599
AlwNI CAGNNNCTG 1 cut(s) 651
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 2 cut(s) 478, 603
ApeKI GCWGC 2 cut(s) 234, 635
ApoI RAATTY 3 cut(s) 104, 225, 329
AsiGI ACCGGT 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 444
AsuHPI GGTGA 1 cut(s) 16
BalI TGGCCA 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 705
BbvI GCAGC 2 cut(s) 246, 647
BccI CCATC 1 cut(s) 650
BcgI CGANNNNNNTGC 2 cut(s) 405, 439
BciT130I CCWGG 1 cut(s) 420
BfaI CTAG 3 cut(s) 75, 326, 539
BisI GCNGC 4 cut(s) 235, 600, 603, 636
BlsI GCNGC 4 cut(s) 236, 601, 604, 637
Bme1390I CCNGG 1 cut(s) 420
BmrFI CCNGG 1 cut(s) 420
BmrI ACTGGG 2 cut(s) 103, 418
BmsI GCATC 3 cut(s) 544, 622, 670
BmuI ACTGGG 2 cut(s) 103, 418
BpiI GAAGAC 1 cut(s) 705
BsaBI GATNNNNATC 1 cut(s) 200
BsaJI CCNNGG 4 cut(s) 142, 600, 606, 687
BsaWI WCCGGW 2 cut(s) 285, 496
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 735
Bse118I RCCGGY 2 cut(s) 285, 728
Bse1I ACTGG 4 cut(s) 98, 238, 364, 413
Bse3DI GCAATG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 200
BseAI TCCGGA 1 cut(s) 496
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 4 cut(s) 142, 600, 606, 687
BseGI GGATG 1 cut(s) 700
BseJI GATNNNNATC 1 cut(s) 200
BseLI CCNNNNNNNGG 2 cut(s) 94, 735
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 1 cut(s) 609
BseNI ACTGG 4 cut(s) 98, 238, 364, 413
BseRI GAGGAG 1 cut(s) 704
BseXI GCAGC 2 cut(s) 246, 647
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 2 cut(s) 480, 605
BshTI ACCGGT 1 cut(s) 285
BsiSI CCGG 4 cut(s) 286, 497, 675, 729
BslI CCNNNNNNNGG 2 cut(s) 94, 735
BsnI GGCC 2 cut(s) 480, 605
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 4 cut(s) 6, 422, 485, 591
Bsp19I CCATGG 1 cut(s) 142
BspACI CCGC 3 cut(s) 511, 600, 602
BspANI GGCC 2 cut(s) 480, 605
BspCNI CTCAG 1 cut(s) 608
BspEI TCCGGA 1 cut(s) 496
BspFNI CGCG 1 cut(s) 602
BspPI GGATC 2 cut(s) 430, 599
BsrDI GCAATG 1 cut(s) 513
BsrFI RCCGGY 2 cut(s) 285, 728
BsrI ACTGG 4 cut(s) 98, 238, 364, 413
BssAI RCCGGY 2 cut(s) 285, 728
BssECI CCNNGG 4 cut(s) 142, 600, 606, 687
BssMI GATC 4 cut(s) 6, 422, 485, 591
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 420
Bst6I CTCTTC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 111, 595
BstDSI CCRYGG 2 cut(s) 142, 600
BstF5I GGATG 1 cut(s) 700
BstFNI CGCG 1 cut(s) 602
BstKTI GATC 4 cut(s) 9, 425, 488, 594
BstMBI GATC 4 cut(s) 6, 422, 485, 591
BstMWI GCNNNNNNNGC 4 cut(s) 532, 635, 680, 729
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 418
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 2 cut(s) 246, 647
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 1 cut(s) 591
BstYI RGATCY 1 cut(s) 591
BsuRI GGCC 2 cut(s) 480, 605
BtgI CCRYGG 2 cut(s) 142, 600
BtsCI GGATG 1 cut(s) 700
BtsIMutI CAGTG 1 cut(s) 357
CaiI CAGNNNCTG 1 cut(s) 651
Cfr10I RCCGGY 2 cut(s) 285, 728
Cfr42I CCGCGG 1 cut(s) 603
CspAI ACCGGT 1 cut(s) 285
CviAII CATG 6 cut(s) 143, 185, 321, 385, 435, 476
DdeI CTNAG 2 cut(s) 111, 595
DpnI GATC 4 cut(s) 8, 424, 487, 593
DpnII GATC 4 cut(s) 6, 422, 485, 591
DraI TTTAAA 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 6 cut(s) 146, 188, 324, 388, 438, 479
FatI CATG 6 cut(s) 142, 184, 320, 384, 434, 475
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 4 cut(s) 235, 600, 603, 636
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 4 cut(s) 235, 600, 603, 636
FspBI CTAG 3 cut(s) 75, 326, 539
GluI GCNGC 4 cut(s) 235, 600, 603, 636
HaeIII GGCC 2 cut(s) 480, 605
HapII CCGG 4 cut(s) 286, 497, 675, 729
Hin1II CATG 6 cut(s) 146, 188, 324, 388, 438, 479
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 1 cut(s) 16
HpaII CCGG 4 cut(s) 286, 497, 675, 729
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 27, 610, 622, 743
Hpy188III TCNNGA 4 cut(s) 155, 326, 449, 497
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 707
HpyAV CCTTC 4 cut(s) 494, 553, 627, 713
HpyCH4V TGCA 3 cut(s) 149, 416, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 532, 635, 680, 729
HpyF3I CTNAG 2 cut(s) 111, 595
Hsp92II CATG 6 cut(s) 146, 188, 324, 388, 438, 479
Kpn2I TCCGGA 1 cut(s) 496
KspI CCGCGG 1 cut(s) 603
Kzo9I GATC 4 cut(s) 6, 422, 485, 591
LmnI GCTCC 2 cut(s) 499, 582
Lsp1109I GCAGC 2 cut(s) 246, 647
LweI GCATC 3 cut(s) 544, 622, 670
MaeI CTAG 3 cut(s) 75, 326, 539
MalI GATC 4 cut(s) 8, 424, 487, 593
MboI GATC 4 cut(s) 6, 422, 485, 591
MboII GAAGA 3 cut(s) 40, 385, 710
MflI RGATCY 1 cut(s) 591
MlsI TGGCCA 1 cut(s) 480
MluCI AATT 8 cut(s) 21, 104, 173, 225, 253, 329, 348, 389
MluNI TGGCCA 1 cut(s) 480
Mox20I TGGCCA 1 cut(s) 480
MroI TCCGGA 1 cut(s) 496
MroXI GAANNNNTTC 1 cut(s) 444
MscI TGGCCA 1 cut(s) 480
MseI TTAA 6 cut(s) 213, 252, 291, 333, 396, 549
Msp20I TGGCCA 1 cut(s) 480
MspA1I CMGCKG 1 cut(s) 602
MspI CCGG 4 cut(s) 286, 497, 675, 729
MspR9I CCNGG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 420
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 4 cut(s) 532, 635, 680, 729
NcoI CCATGG 1 cut(s) 142
NdeI CATATG 1 cut(s) 247
NdeII GATC 4 cut(s) 6, 422, 485, 591
NlaIII CATG 6 cut(s) 146, 188, 324, 388, 438, 479
NspI RCATGY 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 1 cut(s) 16
PinAI ACCGGT 1 cut(s) 285
PkrI GCNGC 4 cut(s) 236, 601, 604, 637
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 651
PsuI RGATCY 1 cut(s) 591
SacII CCGCGG 1 cut(s) 603
SaqAI TTAA 6 cut(s) 213, 252, 291, 333, 396, 549
SatI GCNGC 4 cut(s) 235, 600, 603, 636
Sau3AI GATC 4 cut(s) 6, 422, 485, 591
ScrFI CCNGG 1 cut(s) 420
SetI ASST 9 cut(s) 184, 212, 312, 341, 402, 421, 469, 547, 666
SfaNI GCATC 3 cut(s) 544, 622, 670
Sfr303I CCGCGG 1 cut(s) 603
SgrBI CCGCGG 1 cut(s) 603
Sse9I AATT 8 cut(s) 21, 104, 173, 225, 253, 329, 348, 389
SsiI CCGC 3 cut(s) 511, 600, 602
SspMI CTAG 3 cut(s) 75, 326, 539
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 1 cut(s) 142
TaqI TCGA 1 cut(s) 273
TasI AATT 8 cut(s) 21, 104, 173, 225, 253, 329, 348, 389
TauI GCSGC 2 cut(s) 602, 605
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 6 cut(s) 213, 252, 291, 333, 396, 549
Tru9I TTAA 6 cut(s) 213, 252, 291, 333, 396, 549
TscAI CASTG 1 cut(s) 364
TseI GCWGC 2 cut(s) 234, 635
TspDTI ATGAA 6 cut(s) 234, 312, 386, 477, 711, 723
TspRI CASTG 1 cut(s) 364
XapI RAATTY 3 cut(s) 104, 225, 329
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 1 cut(s) 324
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 3 cut(s) 75, 326, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.