Rmu_sc0000547.1_g000031

endoplasmic reticulum protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000547.1
OG0025094
Physical Location & Seq
Forward (+)
139783 .. 141923
2141 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
atgaatttgatcgatactcctacagagatcaaggaaaccgcaaagcacctgaagttggaatttgagatgaaaggtcttgggagaacaaattattgtctcgacctggagcccgagcacagtgcagatgaaattttggacatctcatttgatgtgaacttgttagctagatataactctgcgccaacacgccgctactggaatggcataaaagacatttttcgctaccttagaggtacggcggatatgggcttattttatccctatgcatcaaggaatggatcaaacccccttgatcctcagaatgatgctcgccttgttggatatgttgatatagactatctatcagacccgcacaaggcatgcatgttccaagattgggttattttgtcccactgggttttgttacctggcaaggttttaacgaggcacatctttagaggtcaccccccacttatactacatcctgaagatgctttgattcgacatatacatgcatttgctcatcttttcccttcgaccacgggactacaggtgtactatgaaaaggcactcgcagcacaatttatcatcctgttaattgacttgaagctcttcgcagttcaagaaagggctgtgacacaagcctgcttcctcagcaccatgacgacatcaaggaggctcactagtaggaaggtggagaggtttgacaagaagattacgaagagaggagctgtgcctcagaaaacagccaaaagggggagggactatcctgttggccctattttgctcggcttctttatctttgtggtcataggatcatctctgtttcagataatcaggagtgctacaagcgggggcatggtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAMP4

Protein Analysis

281

Amino Acids

32.03

Weight (kDa)

9.26

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAMP4 PF06624 218 - 274 1.9e-23 Ribosome associated membrane protein RAMP4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025094)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0308681
rosa_multiflora Rmu_sc0000547.1_g000031
rosa_roxburghii Rroxscaffold_7G00185830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 39, 190, 239, 350, 831
AclWI GGATC 3 cut(s) 286, 287, 802
AcsI RAATTY 3 cut(s) 4, 59, 129
AcuI CTGAAG 2 cut(s) 71, 486
AfaI GTAC 2 cut(s) 235, 536
AfiI CCNNNNNNNGG 4 cut(s) 55, 355, 376, 735
AgsI TTSAA 2 cut(s) 586, 602
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 2 cut(s) 102, 406
AluBI AGCT 3 cut(s) 164, 589, 710
AluI AGCT 3 cut(s) 164, 589, 710
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 3 cut(s) 286, 287, 802
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 1 cut(s) 554
ApoI RAATTY 3 cut(s) 4, 59, 129
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
Asp700I GAANNNNTTC 1 cut(s) 590
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 1 cut(s) 755
AsuHPI GGTGA 1 cut(s) 434
AvaI CYCGRG 1 cut(s) 110
BanII GRGCYC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 117
BbvCI CCTCAGC 1 cut(s) 632
BbvI GCAGC 1 cut(s) 566
BceAI ACGGC 1 cut(s) 252
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 2 cut(s) 104, 408
BcoDI GTCTC 1 cut(s) 101
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 2 cut(s) 165, 663
BfmI CTRYAG 2 cut(s) 21, 527
BisI GCNGC 2 cut(s) 190, 555
BlsI GCNGC 2 cut(s) 191, 556
Bme1390I CCNGG 2 cut(s) 104, 408
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 1 cut(s) 108
BmrFI CCNGG 2 cut(s) 104, 408
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 3 cut(s) 275, 295, 460
BmuI ACTGGG 1 cut(s) 403
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 632
Bsa29I ATCGAT 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 519
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 355, 376, 735
Bse1I ACTGG 2 cut(s) 200, 398
BseBI CCWGG 2 cut(s) 104, 408
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 519
BseGI GGATG 2 cut(s) 460, 567
BseLI CCNNNNNNNGG 4 cut(s) 55, 355, 376, 735
BseMII CTCAG 3 cut(s) 311, 646, 731
BseNI ACTGG 2 cut(s) 200, 398
BseRI GAGGAG 1 cut(s) 720
BseXI GCAGC 1 cut(s) 566
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 1 cut(s) 756
BshVI ATCGAT 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 110
BslFI GGGAC 3 cut(s) 373, 537, 755
BslI CCNNNNNNNGG 4 cut(s) 55, 355, 376, 735
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 3 cut(s) 373, 537, 755
BsnI GGCC 1 cut(s) 756
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 2 cut(s) 111, 117
Bsp143I GATC 5 cut(s) 9, 27, 278, 292, 794
BspACI CCGC 5 cut(s) 39, 190, 239, 350, 831
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 3 cut(s) 310, 645, 730
BspDI ATCGAT 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 3 cut(s) 286, 287, 802
BspQI GCTCTTC 1 cut(s) 596
BsrI ACTGG 2 cut(s) 200, 398
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 5 cut(s) 9, 27, 278, 292, 794
Bst2UI CCWGG 2 cut(s) 104, 408
Bst4CI ACNGT 1 cut(s) 119
Bst6I CTCTTC 2 cut(s) 596, 695
BstC8I GCNNGC 3 cut(s) 310, 361, 625
BstDEI CTNAG 4 cut(s) 227, 297, 632, 717
BstDSI CCRYGG 1 cut(s) 519
BstEII GGTNACC 1 cut(s) 440
BstF5I GGATG 2 cut(s) 460, 567
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 5 cut(s) 12, 30, 281, 295, 797
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 5 cut(s) 9, 27, 278, 292, 794
BstMWI GCNNNNNNNGC 2 cut(s) 554, 633
BstNI CCWGG 2 cut(s) 104, 408
BstNSI RCATGY 3 cut(s) 363, 367, 494
BstPI GGTNACC 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 102, 406
BstSFI CTRYAG 2 cut(s) 21, 527
BstV1I GCAGC 1 cut(s) 566
Bsu15I ATCGAT 1 cut(s) 12
BsuRI GGCC 1 cut(s) 756
BsuTUI ATCGAT 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 2 cut(s) 460, 567
BtsIMutI CAGTG 2 cut(s) 124, 391
Cac8I GCNNGC 3 cut(s) 310, 361, 625
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 755
ClaI ATCGAT 1 cut(s) 12
Csp6I GTAC 2 cut(s) 234, 535
CviAII CATG 5 cut(s) 360, 364, 491, 640, 838
CviQI GTAC 2 cut(s) 234, 535
DdeI CTNAG 4 cut(s) 227, 297, 632, 717
DpnI GATC 5 cut(s) 11, 29, 280, 294, 796
DpnII GATC 5 cut(s) 9, 27, 278, 292, 794
Eam1104I CTCTTC 2 cut(s) 596, 695
EarI CTCTTC 2 cut(s) 596, 695
EciI GGCGGA 1 cut(s) 254
Eco24I GRGCYC 1 cut(s) 111
Eco57I CTGAAG 2 cut(s) 71, 486
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 440
EcoO65I GGTNACC 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 102, 406
EcoT22I ATGCAT 3 cut(s) 268, 365, 496
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 5 cut(s) 363, 367, 494, 643, 841
FaqI GGGAC 3 cut(s) 373, 537, 755
FatI CATG 5 cut(s) 359, 363, 490, 639, 837
FauI CCCGC 2 cut(s) 357, 824
Fnu4HI GCNGC 2 cut(s) 190, 555
FokI GGATG 2 cut(s) 447, 554
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 190, 555
FspBI CTAG 2 cut(s) 165, 663
GlaI GCGC 1 cut(s) 180
GluI GCNGC 2 cut(s) 190, 555
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 1 cut(s) 756
HhaI GCGC 1 cut(s) 181
Hin1II CATG 5 cut(s) 363, 367, 494, 643, 841
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HinfI GANTC 1 cut(s) 478
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 154, 535
Hpy188I TCNGA 4 cut(s) 300, 346, 720, 810
Hpy188III TCNNGA 4 cut(s) 98, 464, 602, 817
Hpy8I GTNNAC 2 cut(s) 154, 535
HpyAV CCTTC 2 cut(s) 522, 664
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4V TGCA 4 cut(s) 122, 266, 363, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 554, 633
HpyF3I CTNAG 4 cut(s) 227, 297, 632, 717
Hsp92II CATG 5 cut(s) 363, 367, 494, 643, 841
HspAI GCGC 1 cut(s) 179
Kzo9I GATC 5 cut(s) 9, 27, 278, 292, 794
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 2 cut(s) 106, 707
Lsp1109I GCAGC 1 cut(s) 566
LweI GCATC 3 cut(s) 275, 295, 460
MaeI CTAG 2 cut(s) 165, 663
MaeIII GTNAC 3 cut(s) 402, 440, 613
MalI GATC 5 cut(s) 11, 29, 280, 294, 796
MboI GATC 5 cut(s) 9, 27, 278, 292, 794
MboII GAAGA 4 cut(s) 479, 583, 703, 712
MhlI GDGCHC 2 cut(s) 111, 117
MluCI AATT 6 cut(s) 4, 59, 88, 129, 560, 576
MmeI TCCRAC 2 cut(s) 36, 298
Mph1103I ATGCAT 3 cut(s) 268, 365, 496
MroXI GAANNNNTTC 1 cut(s) 590
MseI TTAA 2 cut(s) 419, 575
MslI CAYNNNNRTG 1 cut(s) 489
MspR9I CCNGG 2 cut(s) 104, 408
MvaI CCWGG 2 cut(s) 104, 408
MwoI GCNNNNNNNGC 2 cut(s) 554, 633
NdeII GATC 5 cut(s) 9, 27, 278, 292, 794
NlaIII CATG 5 cut(s) 363, 367, 494, 643, 841
NlaIV GGNNCC 1 cut(s) 108
NmeAIII GCCGAG 1 cut(s) 747
NmuCI GTSAC 2 cut(s) 440, 613
NsiI ATGCAT 3 cut(s) 268, 365, 496
NspI RCATGY 3 cut(s) 363, 367, 494
PaeI GCATGC 1 cut(s) 363
PciSI GCTCTTC 1 cut(s) 596
PdmI GAANNNNTTC 1 cut(s) 590
PfeI GAWTC 1 cut(s) 478
PkrI GCNGC 2 cut(s) 191, 556
Psp6I CCWGG 2 cut(s) 102, 406
PspEI GGTNACC 1 cut(s) 440
PspGI CCWGG 2 cut(s) 102, 406
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 1 cut(s) 755
RsaI GTAC 2 cut(s) 235, 536
RsaNI GTAC 2 cut(s) 234, 535
RseI CAYNNNNRTG 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 596
SaqAI TTAA 2 cut(s) 419, 575
SatI GCNGC 2 cut(s) 190, 555
Sau3AI GATC 5 cut(s) 9, 27, 278, 292, 794
Sau96I GGNCC 1 cut(s) 755
ScrFI CCNGG 2 cut(s) 104, 408
SduI GDGCHC 2 cut(s) 111, 117
SfaNI GCATC 3 cut(s) 275, 295, 460
SfcI CTRYAG 2 cut(s) 21, 527
SmiMI CAYNNNNRTG 1 cut(s) 489
SpeI ACTAGT 1 cut(s) 662
SphI GCATGC 1 cut(s) 363
Sse9I AATT 6 cut(s) 4, 59, 88, 129, 560, 576
SsiI CCGC 5 cut(s) 39, 190, 239, 350, 831
SspMI CTAG 2 cut(s) 165, 663
StyD4I CCNGG 2 cut(s) 102, 406
TaaI ACNGT 1 cut(s) 119
TaqI TCGA 4 cut(s) 12, 99, 481, 515
TasI AATT 6 cut(s) 4, 59, 88, 129, 560, 576
TatI WGTACW 1 cut(s) 534
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 2 cut(s) 419, 575
Tru9I TTAA 2 cut(s) 419, 575
TscAI CASTG 2 cut(s) 124, 398
TseFI GTSAC 2 cut(s) 440, 613
TseI GCWGC 1 cut(s) 554
Tsp45I GTSAC 2 cut(s) 440, 613
TspDTI ATGAA 4 cut(s) 17, 83, 141, 555
TspRI CASTG 2 cut(s) 124, 398
XapI RAATTY 3 cut(s) 4, 59, 129
XceI RCATGY 3 cut(s) 363, 367, 494
XmnI GAANNNNTTC 1 cut(s) 590
XspI CTAG 2 cut(s) 165, 663
Zsp2I ATGCAT 3 cut(s) 268, 365, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.