Rmu_sc0000766.1_g000043

LIMR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000766.1
OG0025491
Physical Location & Seq
Forward (+)
244493 .. 245333
841 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
atgggggatttcaacctcgcgctcgtaatcgtcgccattatactggtggtcgttgtcttcatcttcaatgtctacctcctcgtcaattaccagcacccggacgacaagaaccaggcctactttcccaaattcgtcgtcgttttggggctctccgtcgccgcgatttccattctcatgttaccggcagacgtggccaaccggcaggcctgccgccacgcgatatacaatggcgcgtgtaatctcactcttccgatgaaggatctctggctcgccgtctatatcctcgacgccgtcctcgtcttcctcatcattcccttcgctatgttctactacgaaggcgaccaggacaagactgttggaaagagaattggaagcgcaatgttgtgggtgggggtaagcgccgttgtgtgtggtctcgttctcggcatcttgtacgggcttgttggaaaggtggactttactgttaggcacctctcttcttccacagctgcatttcctagttcattttcctattctagcggtgaaccatgtatcagtggctcgaaacaggtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04977 map04977
Pfam Domains
Protein Families
LMBR1

Protein Analysis

184

Amino Acids

20.0

Weight (kDa)

6.8

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LMBR1 PF04791 9 - 154 1.7e-33 LMBR1-like membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025491)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8032496.1_g000001 Rmu_co8091672.1_g000001 Rmu_sc0000766.1_g000043

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 468
AccI GTMKAC 1 cut(s) 72
AccII CGCG 4 cut(s) 20, 161, 218, 233
AciI CCGC 3 cut(s) 159, 211, 519
AclWI GGATC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 1 cut(s) 128
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 2 cut(s) 13, 67
AjiI CACGTC 1 cut(s) 190
AjnI CCWGG 2 cut(s) 111, 342
AluBI AGCT 1 cut(s) 488
AluI AGCT 1 cut(s) 488
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 1 cut(s) 267
AoxI GGCC 3 cut(s) 114, 192, 204
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AspLEI GCGC 4 cut(s) 22, 233, 377, 401
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 533
BalI TGGCCA 1 cut(s) 194
BanI GGYRCC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 2 cut(s) 49, 292
BbvI GCAGC 1 cut(s) 475
BceAI ACGGC 3 cut(s) 257, 275, 386
BciT130I CCWGG 2 cut(s) 113, 344
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 2 cut(s) 498, 516
BfoI RGCGCY 1 cut(s) 402
BisI GCNGC 3 cut(s) 159, 211, 489
BlsI GCNGC 3 cut(s) 160, 212, 490
Bme1390I CCNGG 3 cut(s) 98, 113, 344
BmgBI CACGTC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 470
BmrFI CCNGG 3 cut(s) 98, 113, 344
BmsI GCATC 1 cut(s) 435
BpiI GAAGAC 2 cut(s) 49, 292
BpuMI CCSGG 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 419
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse118I RCCGGY 2 cut(s) 181, 198
Bse1I ACTGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 2 cut(s) 113, 344
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 384
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 475
Bsh1236I CGCG 4 cut(s) 20, 161, 218, 233
BshFI GGCC 3 cut(s) 116, 194, 206
BshNI GGYRCC 1 cut(s) 468
BsiSI CCGG 3 cut(s) 98, 182, 199
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 419
BsnI GGCC 3 cut(s) 116, 194, 206
Bso31I GGTCTC 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 3 cut(s) 159, 211, 519
BspANI GGCC 3 cut(s) 116, 194, 206
BspFNI CGCG 4 cut(s) 20, 161, 218, 233
BspLI GGNNCC 1 cut(s) 470
BspPI GGATC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 384
BsrFI RCCGGY 2 cut(s) 181, 198
BsrI ACTGG 1 cut(s) 48
BssAI RCCGGY 2 cut(s) 181, 198
BssMI GATC 1 cut(s) 259
BssNI GRCGYC 1 cut(s) 288
Bst2UI CCWGG 2 cut(s) 113, 344
Bst4CI ACNGT 2 cut(s) 355, 463
Bst6I CTCTTC 2 cut(s) 252, 481
BstACI GRCGYC 1 cut(s) 288
BstC8I GCNNGC 3 cut(s) 204, 208, 270
BstFNI CGCG 4 cut(s) 20, 161, 218, 233
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 4 cut(s) 22, 233, 377, 401
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 2 cut(s) 113, 344
BstSCI CCNGG 3 cut(s) 96, 111, 342
BstUI CGCG 4 cut(s) 20, 161, 218, 233
BstV1I GCAGC 1 cut(s) 475
BstV2I GAAGAC 2 cut(s) 49, 292
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 1 cut(s) 259
BsuRI GGCC 3 cut(s) 116, 194, 206
BtrI CACGTC 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 541
Cac8I GCNNGC 3 cut(s) 204, 208, 270
CfoI GCGC 4 cut(s) 22, 233, 377, 401
Cfr10I RCCGGY 2 cut(s) 181, 198
CseI GACGC 1 cut(s) 296
Csp6I GTAC 1 cut(s) 433
CviAII CATG 2 cut(s) 175, 528
CviJI RGCY 8 cut(s) 116, 148, 194, 206, 268, 439, 488, 540
CviKI_1 RGCY 8 cut(s) 116, 148, 194, 206, 268, 439, 488, 540
CviQI GTAC 1 cut(s) 433
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 252, 481
EarI CTCTTC 2 cut(s) 252, 481
Eco147I AGGCCT 2 cut(s) 116, 206
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 111, 342
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 2 cut(s) 178, 531
FaiI YATR 7 cut(s) 41, 176, 223, 279, 323, 529, 553
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FatI CATG 2 cut(s) 174, 527
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 159, 211, 489
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 3 cut(s) 159, 211, 489
FspBI CTAG 2 cut(s) 498, 516
GlaI GCGC 4 cut(s) 21, 232, 376, 400
GluI GCNGC 3 cut(s) 159, 211, 489
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 3 cut(s) 116, 194, 206
HapII CCGG 3 cut(s) 98, 182, 199
HgaI GACGC 1 cut(s) 296
HhaI GCGC 4 cut(s) 22, 233, 377, 401
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 2 cut(s) 178, 531
Hin6I GCGC 4 cut(s) 20, 231, 375, 399
HinP1I GCGC 4 cut(s) 20, 231, 375, 399
HpaII CCGG 3 cut(s) 98, 182, 199
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 3 cut(s) 73, 454, 524
Hpy188I TCNGA 1 cut(s) 252
Hpy8I GTNNAC 3 cut(s) 73, 454, 524
Hpy99I CGWCG 5 cut(s) 35, 137, 140, 158, 290
HpyAV CCTTC 3 cut(s) 250, 325, 329
HpyCH4III ACNGT 2 cut(s) 355, 463
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 2 cut(s) 178, 531
HspAI GCGC 4 cut(s) 20, 231, 375, 399
Kzo9I GATC 1 cut(s) 259
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 1 cut(s) 435
MaeI CTAG 2 cut(s) 498, 516
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 1 cut(s) 177
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 6 cut(s) 49, 55, 239, 292, 468, 471
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 3 cut(s) 85, 128, 366
MluNI TGGCCA 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 337, 424
MnlI CCTC 7 cut(s) 26, 86, 89, 293, 305, 314, 482
Mox20I TGGCCA 1 cut(s) 194
MscI TGGCCA 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 173
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 3 cut(s) 98, 182, 199
MspR9I CCNGG 3 cut(s) 98, 113, 344
MvaI CCWGG 2 cut(s) 113, 344
MvnI CGCG 4 cut(s) 20, 161, 218, 233
MwoI GCNNNNNNNGC 1 cut(s) 191
NciI CCSGG 1 cut(s) 98
NdeII GATC 1 cut(s) 259
NlaIII CATG 2 cut(s) 178, 531
NlaIV GGNNCC 1 cut(s) 470
NmeAIII GCCGAG 1 cut(s) 402
PceI AGGCCT 2 cut(s) 116, 206
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PflFI GACNNNGTC 1 cut(s) 290
PkrI GCNGC 3 cut(s) 160, 212, 490
Psp6I CCWGG 2 cut(s) 111, 342
PspGI CCWGG 2 cut(s) 111, 342
PspN4I GGNNCC 1 cut(s) 470
PsrI GAACNNNNNNTAC 2 cut(s) 101, 133
PsuI RGATCY 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 173
SatI GCNGC 3 cut(s) 159, 211, 489
Sau3AI GATC 1 cut(s) 259
ScrFI CCNGG 3 cut(s) 98, 113, 344
SduI GDGCHC 1 cut(s) 150
SetI ASST 7 cut(s) 18, 78, 192, 453, 474, 490, 552
SfaNI GCATC 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 3 cut(s) 85, 128, 366
SseBI AGGCCT 2 cut(s) 116, 206
SsiI CCGC 3 cut(s) 159, 211, 519
SspMI CTAG 2 cut(s) 498, 516
StuI AGGCCT 2 cut(s) 116, 206
StyD4I CCNGG 3 cut(s) 96, 111, 342
TaaI ACNGT 2 cut(s) 355, 463
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 285, 542
TasI AATT 3 cut(s) 85, 128, 366
TauI GCSGC 2 cut(s) 161, 213
TscAI CASTG 1 cut(s) 541
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 3 cut(s) 49, 269, 492
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 290
XapI RAATTY 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 2 cut(s) 498, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.