Rmu_sc0001557.1_g000001

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001557.1
OG0023073
Physical Location & Seq
Forward (+)
5534 .. 6493
960 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 324 bp
atgagtatcgagcctttcaataggttggtgaagctcgcggcgagggcgttctacgatgacataaccacgaaaggggataaccagcccaagactggccggagcgacaatcggggaattgccgtggttgtgctcgacgcgctctcaaggcgacaatgggtgagagaagaggatttggcaaaggacttgaaactgcactcgaagcaacttcgtcgtactttgcgattttttgaggaggagaaactggtcactcgagaccataggaaggagacagctaagggtgcaaagatatttagtgctgcagttacagatgctgttaatacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

107

Amino Acids

12.27

Weight (kDa)

9.91

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 40 - 93 3.2e-07 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023073)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001557.1_g000001 Rmu_sc0001557.1_g000002
rosa_samantha Rh7CG068700 Rh7DG068100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 38, 137
AciI CCGC 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 94
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 3 cut(s) 72, 92, 262
AgsI TTSAA 2 cut(s) 19, 187
AluBI AGCT 2 cut(s) 34, 272
AluI AGCT 2 cut(s) 34, 272
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 246, 260
AlwNI CAGNNNCTG 1 cut(s) 311
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 139
AsuHPI GGTGA 2 cut(s) 40, 169
AvaI CYCGRG 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 283
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BcoDI GTCTC 2 cut(s) 246, 260
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 2 cut(s) 39, 297
BlsI GCNGC 2 cut(s) 40, 298
BmeT110I CYCGRG 1 cut(s) 249
BmsI GCATC 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 127
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 92, 262
Bse1I ACTGG 2 cut(s) 97, 246
BseDI CCNNGG 1 cut(s) 120
BseLI CCNNNNNNNGG 3 cut(s) 72, 92, 262
BseNI ACTGG 2 cut(s) 97, 246
BseRI GAGGAG 2 cut(s) 245, 248
BseXI GCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 176
Bsh1236I CGCG 2 cut(s) 38, 137
BshFI GGCC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 1 cut(s) 97
BslI CCNNNNNNNGG 3 cut(s) 72, 92, 262
BsmAI GTCTC 2 cut(s) 246, 260
BsnI GGCC 1 cut(s) 96
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 132
BspACI CCGC 1 cut(s) 38
BspANI GGCC 1 cut(s) 96
BspFNI CGCG 2 cut(s) 38, 137
BspMAI CTGCAG 1 cut(s) 301
BspTNI GGTCTC 1 cut(s) 246
BsrI ACTGG 2 cut(s) 97, 246
BssECI CCNNGG 1 cut(s) 120
Bst6I CTCTTC 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 36
BstDEI CTNAG 1 cut(s) 273
BstDSI CCRYGG 1 cut(s) 120
BstFNI CGCG 2 cut(s) 38, 137
BstHHI GCGC 1 cut(s) 139
BstMAI GTCTC 2 cut(s) 246, 260
BstMWI GCNNNNNNNGC 5 cut(s) 44, 136, 145, 199, 278
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 2 cut(s) 38, 137
BstV1I GCAGC 1 cut(s) 283
BsuRI GGCC 1 cut(s) 96
BtgI CCRYGG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 36
CaiI CAGNNNCTG 1 cut(s) 311
CfoI GCGC 1 cut(s) 139
CseI GACGC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 213
CviJI RGCY 5 cut(s) 13, 34, 85, 96, 272
CviKI_1 RGCY 5 cut(s) 13, 34, 85, 96, 272
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 1 cut(s) 273
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 249
FaiI YATR 2 cut(s) 62, 258
Fnu4HI GCNGC 2 cut(s) 39, 297
Fsp4HI GCNGC 2 cut(s) 39, 297
GlaI GCGC 1 cut(s) 138
GluI GCNGC 2 cut(s) 39, 297
HaeIII GGCC 1 cut(s) 96
HapII CCGG 1 cut(s) 97
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 139
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HpaII CCGG 1 cut(s) 97
HphI GGTGA 2 cut(s) 40, 169
Hpy188III TCNNGA 1 cut(s) 251
Hpy99I CGWCG 2 cut(s) 137, 213
HpyAV CCTTC 1 cut(s) 256
HpyCH4V TGCA 3 cut(s) 193, 281, 299
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 136, 145, 199, 278
HpyF3I CTNAG 1 cut(s) 273
HspAI GCGC 1 cut(s) 137
LmnI GCTCC 1 cut(s) 99
LpnPI CCDG 4 cut(s) 78, 95, 110, 227
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 1 cut(s) 298
MaeIII GTNAC 2 cut(s) 244, 301
MboII GAAGA 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 1 cut(s) 114
MnlI CCTC 4 cut(s) 36, 160, 223, 226
MseI TTAA 2 cut(s) 315, 322
MspI CCGG 1 cut(s) 97
MvnI CGCG 2 cut(s) 38, 137
MwoI GCNNNNNNNGC 5 cut(s) 44, 136, 145, 199, 278
NmuCI GTSAC 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PkrI GCNGC 2 cut(s) 40, 298
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 311
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SaqAI TTAA 2 cut(s) 315, 322
SatI GCNGC 2 cut(s) 39, 297
SduI GDGCHC 1 cut(s) 132
SetI ASST 3 cut(s) 26, 36, 274
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 1 cut(s) 297
Sfr274I CTCGAG 1 cut(s) 249
SlaI CTCGAG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 142, 249
SmoI CTYRAG 2 cut(s) 142, 249
Sse9I AATT 1 cut(s) 114
SsiI CCGC 1 cut(s) 38
TaqI TCGA 4 cut(s) 9, 132, 197, 250
TasI AATT 1 cut(s) 114
TauI GCSGC 1 cut(s) 41
Tru1I TTAA 2 cut(s) 315, 322
Tru9I TTAA 2 cut(s) 315, 322
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 1 cut(s) 244
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XhoI CTCGAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.