Rmu_sc0001634.1_g000032

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001634.1
OG0031146
Physical Location & Seq
Forward (+)
184464 .. 186070
1607 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
atgttgacaaaccataccttccaagcttcgaaaaccaaagccatcctaaccctcttgcaaggatgcaacaccctcaaaaggctcagaaaaatccacgcccacgtcatcaccaacggtcttcaacaccacccttccatttgcaacaagctcctcaacttctgcgccgtctccgtgtcaggcagcttaccctacgctcagctgctcttccattatcacatcccaaacccacaaacccaagactggaattccatgatcagaggtgatcaggttcacaaatttgtagtggatgacaagtcacatccagatactgaagaaatctatcgaaaattggaggtagtagtccaacgaactgctctacatggttatctgcaagaggaatctcttatcagagcctgtgaatttacctccatagacaccaactgttcgggaacttcaggttcatgtcacagccgtcggagtccggtcaccggtcgccggattttggtcaactttcgcaggattccggccacctgtcgccggattccggtcatcggctaccgcccatcagaatttgctaaaaatctcattggaaagttttttgcctccaatagacatttattgccccctaatagacgtctattgccccccaataggactttcagttgccagaatgggaactattctccctaa

Protein Analysis

222

Amino Acids

25.27

Weight (kDa)

9.96

Isoelectric Point (pI)

56.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Eplus_motif PF20430 90 - 116 2.8e-07 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0031146)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001634.1_g000032 Rmu_sc0022983.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 616
AciI CCGC 1 cut(s) 538
AcoI YGGCCR 1 cut(s) 504
AcsI RAATTY 4 cut(s) 244, 275, 398, 548
AcuI CTGAAG 2 cut(s) 330, 417
AcyI GRCGYC 1 cut(s) 613
AfiI CCNNNNNNNGG 9 cut(s) 78, 240, 467, 474, 481, 516, 523, 530, 630
AgeI ACCGGT 1 cut(s) 467
AgsI TTSAA 1 cut(s) 122
AjiI CACGTC 1 cut(s) 103
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AluBI AGCT 4 cut(s) 26, 148, 183, 199
AluI AGCT 4 cut(s) 26, 148, 183, 199
Alw26I GTCTC 1 cut(s) 172
AlwNI CAGNNNCTG 2 cut(s) 308, 393
AoxI GGCC 1 cut(s) 504
ApeKI GCWGC 2 cut(s) 180, 199
ApoI RAATTY 4 cut(s) 244, 275, 398, 548
AsiGI ACCGGT 1 cut(s) 467
AspLEI GCGC 1 cut(s) 164
AsuHPI GGTGA 3 cut(s) 100, 272, 457
AsuII TTCGAA 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 186, 192
BccI CCATC 2 cut(s) 50, 550
BceAI ACGGC 2 cut(s) 149, 435
BclI TGATCA 2 cut(s) 252, 262
BcoDI GTCTC 1 cut(s) 172
BisI GCNGC 2 cut(s) 181, 200
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 2 cut(s) 182, 201
BmgBI CACGTC 1 cut(s) 103
BmsI GCATC 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 29
BsaHI GRCGYC 1 cut(s) 613
BsaWI WCCGGW 3 cut(s) 460, 467, 523
Bsc4I CCNNNNNNNGG 9 cut(s) 78, 240, 467, 474, 481, 516, 523, 530, 630
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 1 cut(s) 245
BseGI GGATG 5 cut(s) 42, 68, 216, 292, 298
BseLI CCNNNNNNNGG 9 cut(s) 78, 240, 467, 474, 481, 516, 523, 530, 630
BseMII CTCAG 2 cut(s) 97, 209
BseNI ACTGG 1 cut(s) 245
BseRI GAGGAG 1 cut(s) 140
BseXI GCAGC 2 cut(s) 186, 192
Bsh1285I CGRYCG 1 cut(s) 472
BshFI GGCC 1 cut(s) 506
BshTI ACCGGT 1 cut(s) 467
BsiEI CGRYCG 1 cut(s) 472
BsiSI CCGG 6 cut(s) 461, 468, 475, 503, 517, 524
BslI CCNNNNNNNGG 9 cut(s) 78, 240, 467, 474, 481, 516, 523, 530, 630
BsmAI GTCTC 1 cut(s) 172
BsmBI CGTCTC 1 cut(s) 172
BsnI GGCC 1 cut(s) 506
Bsp119I TTCGAA 1 cut(s) 29
Bsp143I GATC 2 cut(s) 252, 262
Bsp1720I GCTNAGC 1 cut(s) 195
BspACI CCGC 1 cut(s) 538
BspANI GGCC 1 cut(s) 506
BspCNI CTCAG 2 cut(s) 96, 208
BspQI GCTCTTC 1 cut(s) 209
BspT104I TTCGAA 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 1 cut(s) 245
BssAI RCCGGY 1 cut(s) 467
BssMI GATC 2 cut(s) 252, 262
BssNI GRCGYC 1 cut(s) 613
Bst4CI ACNGT 2 cut(s) 116, 422
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 613
BstBI TTCGAA 1 cut(s) 29
BstDEI CTNAG 2 cut(s) 83, 195
BstEII GGTNACC 1 cut(s) 463
BstF5I GGATG 5 cut(s) 42, 68, 216, 292, 298
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 2 cut(s) 255, 265
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 2 cut(s) 252, 262
BstMCI CGRYCG 1 cut(s) 472
BstPI GGTNACC 1 cut(s) 463
BstV1I GCAGC 2 cut(s) 186, 192
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 1 cut(s) 506
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 5 cut(s) 42, 68, 216, 292, 298
CaiI CAGNNNCTG 2 cut(s) 308, 393
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 467
CspAI ACCGGT 1 cut(s) 467
CviAII CATG 3 cut(s) 250, 359, 441
DdeI CTNAG 2 cut(s) 83, 195
DpnI GATC 2 cut(s) 254, 264
DpnII GATC 2 cut(s) 252, 262
EaeI YGGCCR 1 cut(s) 504
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
Eco57I CTGAAG 2 cut(s) 330, 417
Eco91I GGTNACC 1 cut(s) 463
EcoO65I GGTNACC 1 cut(s) 463
EcoRI GAATTC 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 3 cut(s) 253, 362, 444
FaiI YATR 5 cut(s) 15, 251, 360, 410, 442
FatI CATG 3 cut(s) 249, 358, 440
FbaI TGATCA 2 cut(s) 252, 262
Fnu4HI GCNGC 2 cut(s) 181, 200
FokI GGATG 5 cut(s) 29, 75, 203, 285, 299
Fsp4HI GCNGC 2 cut(s) 181, 200
GlaI GCGC 1 cut(s) 163
GluI GCNGC 2 cut(s) 181, 200
HaeIII GGCC 1 cut(s) 506
HapII CCGG 6 cut(s) 461, 468, 475, 503, 517, 524
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 613
Hin1II CATG 3 cut(s) 253, 362, 444
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 2 cut(s) 6, 487
HindII GTYRAC 2 cut(s) 6, 487
HindIII AAGCTT 1 cut(s) 24
HinfI GANTC 4 cut(s) 377, 457, 499, 520
HpaII CCGG 6 cut(s) 461, 468, 475, 503, 517, 524
HphI GGTGA 3 cut(s) 100, 272, 457
Hpy166II GTNNAC 3 cut(s) 6, 271, 487
Hpy188I TCNGA 5 cut(s) 86, 257, 389, 456, 547
Hpy188III TCNNGA 2 cut(s) 302, 426
Hpy8I GTNNAC 3 cut(s) 6, 271, 487
Hpy99I CGWCG 1 cut(s) 456
HpyAV CCTTC 2 cut(s) 28, 141
HpyCH4III ACNGT 2 cut(s) 116, 422
HpyCH4IV ACGT 2 cut(s) 102, 613
HpyCH4V TGCA 4 cut(s) 58, 66, 141, 370
HpyF3I CTNAG 2 cut(s) 83, 195
HpySE526I ACGT 2 cut(s) 102, 613
Hsp92I GRCGYC 1 cut(s) 613
Hsp92II CATG 3 cut(s) 253, 362, 444
HspAI GCGC 1 cut(s) 162
Ksp22I TGATCA 2 cut(s) 252, 262
Kzo9I GATC 2 cut(s) 252, 262
LguI GCTCTTC 1 cut(s) 209
LmnI GCTCC 1 cut(s) 153
Lsp1109I GCAGC 2 cut(s) 186, 192
LweI GCATC 1 cut(s) 53
MaeII ACGT 2 cut(s) 102, 613
MaeIII GTNAC 3 cut(s) 294, 443, 463
MalI GATC 2 cut(s) 254, 264
MboI GATC 2 cut(s) 252, 262
MboII GAAGA 3 cut(s) 110, 196, 323
MluCI AATT 5 cut(s) 244, 275, 326, 398, 548
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 2 cut(s) 367, 434
MnlI CCTC 8 cut(s) 62, 83, 161, 251, 325, 367, 415, 592
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 6 cut(s) 461, 468, 475, 503, 517, 524
NdeII GATC 2 cut(s) 252, 262
NlaIII CATG 3 cut(s) 253, 362, 444
NmuCI GTSAC 3 cut(s) 294, 443, 463
NspV TTCGAA 1 cut(s) 29
PciSI GCTCTTC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 377, 499, 520
PinAI ACCGGT 1 cut(s) 467
PkrI GCNGC 2 cut(s) 182, 201
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PspEI GGTNACC 1 cut(s) 463
PstNI CAGNNNCTG 2 cut(s) 308, 393
PvuII CAGCTG 1 cut(s) 199
SapI GCTCTTC 1 cut(s) 209
SatI GCNGC 2 cut(s) 181, 200
Sau3AI GATC 2 cut(s) 252, 262
SchI GAGTC 1 cut(s) 466
SfaNI GCATC 1 cut(s) 53
SfuI TTCGAA 1 cut(s) 29
Sse9I AATT 5 cut(s) 244, 275, 326, 398, 548
SsiI CCGC 1 cut(s) 538
TaaI ACNGT 2 cut(s) 116, 422
TaiI ACGT 2 cut(s) 105, 616
TaqI TCGA 2 cut(s) 29, 322
TasI AATT 5 cut(s) 244, 275, 326, 398, 548
TfiI GAWTC 3 cut(s) 377, 499, 520
TseFI GTSAC 3 cut(s) 294, 443, 463
TseI GCWGC 2 cut(s) 180, 199
Tsp45I GTSAC 3 cut(s) 294, 443, 463
TspDTI ATGAA 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 160
XapI RAATTY 4 cut(s) 244, 275, 398, 548
ZraI GACGTC 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.