Rmu_sc0001975.1_g000007

Pre-mRNA branch site p14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001975.1
OG0031175
Physical Location & Seq
Forward (+)
21903 .. 22229
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001975.1_g000007.1.cds

Sequence Viewer

Length: 327 bp
atgaacgcaatcagtctccaggtcaacacgtggctgctgctggaggtcaaccgggtcatctacgtccgctaccgccccttcaacatatccaacggacgagatgtacgatatattcggcaaatacggccacgcgcggcacggcgcgcgtttgtgttctacgaggacatctttgacgccaagacggccgtcgacctcatttctgtctccaaccgctgcctgatcgttcttaattaccagctggccaagacgggccggaagatagaccagaagaaatcactaggatcctggagaaaaataagcaattccttatttccttattctagttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

108

Amino Acids

12.69

Weight (kDa)

10.59

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031175)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001975.1_g000007 Rmu_sc0005532.1_g000010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 189
AccII CGCG 4 cut(s) 132, 134, 144, 146
AciI CCGC 4 cut(s) 67, 73, 134, 211
AclWI GGATC 2 cut(s) 276, 289
AcoI YGGCCR 3 cut(s) 125, 183, 240
AcvI CACGTG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 1 cut(s) 105
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 2 cut(s) 18, 284
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
Alw26I GTCTC 2 cut(s) 20, 208
AlwI GGATC 2 cut(s) 276, 289
AoxI GGCC 4 cut(s) 125, 183, 240, 250
ApeKI GCWGC 3 cut(s) 34, 37, 213
AspLEI GCGC 3 cut(s) 134, 144, 146
AspS9I GGNCC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 53
BalI TGGCCA 1 cut(s) 242
BamHI GGATCC 1 cut(s) 281
BbrPI CACGTG 1 cut(s) 30
BbvI GCAGC 3 cut(s) 21, 24, 200
BceAI ACGGC 4 cut(s) 140, 155, 170, 198
BciT130I CCWGG 2 cut(s) 20, 286
BcnI CCSGG 1 cut(s) 53
BcoDI GTCTC 2 cut(s) 20, 208
BfaI CTAG 2 cut(s) 278, 321
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 4 cut(s) 35, 38, 135, 214
BlsI GCNGC 4 cut(s) 36, 39, 136, 215
Bme1390I CCNGG 3 cut(s) 20, 53, 286
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 283
BmrFI CCNGG 3 cut(s) 20, 53, 286
BoxI GACNNNNGTC 1 cut(s) 185
BpmI CTGGAG 2 cut(s) 62, 307
BpuMI CCSGG 1 cut(s) 53
BsaAI YACGTR 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 174
BseBI CCWGG 2 cut(s) 20, 286
BsePI GCGCGC 1 cut(s) 142
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 3 cut(s) 21, 24, 200
Bsh1236I CGCG 4 cut(s) 132, 134, 144, 146
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 4 cut(s) 127, 185, 242, 252
BsiEI CGRYCG 1 cut(s) 186
BsiSI CCGG 2 cut(s) 52, 253
BsmAI GTCTC 2 cut(s) 20, 208
BsnI GGCC 4 cut(s) 127, 185, 242, 252
Bsp143I GATC 2 cut(s) 219, 281
BspACI CCGC 4 cut(s) 67, 73, 134, 211
BspANI GGCC 4 cut(s) 127, 185, 242, 252
BspFNI CGCG 4 cut(s) 132, 134, 144, 146
BspLI GGNNCC 1 cut(s) 283
BspPI GGATC 2 cut(s) 276, 289
BssHII GCGCGC 1 cut(s) 142
BssMI GATC 2 cut(s) 219, 281
BssNI GRCGYC 1 cut(s) 174
Bst2UI CCWGG 2 cut(s) 20, 286
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 144, 240
BstFNI CGCG 4 cut(s) 132, 134, 144, 146
BstHHI GCGC 3 cut(s) 134, 144, 146
BstKTI GATC 2 cut(s) 222, 284
BstMAI GTCTC 2 cut(s) 20, 208
BstMBI GATC 2 cut(s) 219, 281
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 3 cut(s) 124, 143, 182
BstNI CCWGG 2 cut(s) 20, 286
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 3 cut(s) 18, 51, 284
BstUI CGCG 4 cut(s) 132, 134, 144, 146
BstV1I GCAGC 3 cut(s) 21, 24, 200
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
BstZI CGGCCG 1 cut(s) 183
BsuRI GGCC 4 cut(s) 127, 185, 242, 252
Cac8I GCNNGC 2 cut(s) 144, 240
CfoI GCGC 3 cut(s) 134, 144, 146
Cfr13I GGNCC 1 cut(s) 250
CseI GACGC 1 cut(s) 182
Csp6I GTAC 1 cut(s) 104
CviJI RGCY 6 cut(s) 34, 127, 185, 238, 242, 252
CviKI_1 RGCY 6 cut(s) 34, 127, 185, 238, 242, 252
CviQI GTAC 1 cut(s) 104
DpnI GATC 2 cut(s) 221, 283
DpnII GATC 2 cut(s) 219, 281
EaeI YGGCCR 3 cut(s) 125, 183, 240
EagI CGGCCG 1 cut(s) 183
EclXI CGGCCG 1 cut(s) 183
Eco52I CGGCCG 1 cut(s) 183
Eco72I CACGTG 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 18, 284
FaiI YATR 2 cut(s) 86, 111
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 4 cut(s) 35, 38, 135, 214
Fsp4HI GCNGC 4 cut(s) 35, 38, 135, 214
FspBI CTAG 2 cut(s) 278, 321
GlaI GCGC 3 cut(s) 133, 143, 145
GluI GCNGC 4 cut(s) 35, 38, 135, 214
GsuI CTGGAG 2 cut(s) 62, 307
HaeIII GGCC 4 cut(s) 127, 185, 242, 252
HapII CCGG 2 cut(s) 52, 253
HgaI GACGC 1 cut(s) 182
HhaI GCGC 3 cut(s) 134, 144, 146
Hin1I GRCGYC 1 cut(s) 174
Hin6I GCGC 3 cut(s) 132, 142, 144
HinP1I GCGC 3 cut(s) 132, 142, 144
HincII GTYRAC 3 cut(s) 25, 49, 190
HindII GTYRAC 3 cut(s) 25, 49, 190
HpaII CCGG 2 cut(s) 52, 253
Hpy166II GTNNAC 3 cut(s) 25, 49, 190
Hpy8I GTNNAC 3 cut(s) 25, 49, 190
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 88
HpyCH4IV ACGT 2 cut(s) 29, 63
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 143, 182
HpySE526I ACGT 2 cut(s) 29, 63
Hsp92I GRCGYC 1 cut(s) 174
HspAI GCGC 3 cut(s) 132, 142, 144
Kzo9I GATC 2 cut(s) 219, 281
Lsp1109I GCAGC 3 cut(s) 21, 24, 200
MaeI CTAG 2 cut(s) 278, 321
MaeII ACGT 2 cut(s) 29, 63
MalI GATC 2 cut(s) 221, 283
MboI GATC 2 cut(s) 219, 281
MboII GAAGA 2 cut(s) 268, 280
MflI RGATCY 1 cut(s) 281
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 2 cut(s) 229, 301
MluNI TGGCCA 1 cut(s) 242
MmeI TCCRAC 2 cut(s) 114, 231
MnlI CCTC 3 cut(s) 37, 154, 203
Mox20I TGGCCA 1 cut(s) 242
MscI TGGCCA 1 cut(s) 242
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 2 cut(s) 213, 238
MspI CCGG 2 cut(s) 52, 253
MspR9I CCNGG 3 cut(s) 20, 53, 286
MvaI CCWGG 2 cut(s) 20, 286
MvnI CGCG 4 cut(s) 132, 134, 144, 146
MwoI GCNNNNNNNGC 3 cut(s) 124, 143, 182
NciI CCSGG 1 cut(s) 53
NdeII GATC 2 cut(s) 219, 281
NlaIV GGNNCC 1 cut(s) 283
PauI GCGCGC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 284
PkrI GCNGC 4 cut(s) 36, 39, 136, 215
PmaCI CACGTG 1 cut(s) 30
PmlI CACGTG 1 cut(s) 30
Ppu21I YACGTR 1 cut(s) 30
PshAI GACNNNNGTC 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 18, 284
PspCI CACGTG 1 cut(s) 30
PspGI CCWGG 2 cut(s) 18, 284
PspN4I GGNNCC 1 cut(s) 283
PspPI GGNCC 1 cut(s) 250
PsuI RGATCY 1 cut(s) 281
PteI GCGCGC 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 238
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
SalI GTCGAC 1 cut(s) 188
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 35, 38, 135, 214
Sau3AI GATC 2 cut(s) 219, 281
Sau96I GGNCC 1 cut(s) 250
ScrFI CCNGG 3 cut(s) 20, 53, 286
SetI ASST 6 cut(s) 24, 32, 48, 66, 195, 240
Sse9I AATT 2 cut(s) 229, 301
SsiI CCGC 4 cut(s) 67, 73, 134, 211
SspMI CTAG 2 cut(s) 278, 321
StyD4I CCNGG 3 cut(s) 18, 51, 284
TaiI ACGT 2 cut(s) 32, 66
TaqI TCGA 1 cut(s) 189
TasI AATT 2 cut(s) 229, 301
TauI GCSGC 1 cut(s) 137
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 3 cut(s) 34, 37, 213
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 2 cut(s) 278, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.