Rmu_sc0002028.1_g000013

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002028.1
OG0031180
Physical Location & Seq
Forward (+)
58514 .. 58955
442 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 291 bp
atgcaggcaatgattattcctgcttctaaggaagaaggaattctaattaagaagagctatgctgtctttaatgatgatgggacccttgctgaacttaaaggttttgagatcaaacgtagaggtgagctgaagatcatcaaagttttccagactgaccaggtcaactcacagaggacaagtcagatcgatgaaattgtaaagtatgaagttacacaggggcttttaggggagaaggacataggcagctacgtagaggaggagagaagtttgcaaagactaaaaatagtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000228 GO:0000278 GO:0000731 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006272 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008296 GO:0008297 GO:0008310 GO:0008408 GO:0008622 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009987 GO:0010015 GO:0010086 GO:0016020 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0018130 GO:0019438 GO:0022402 GO:0022616 GO:0022622 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044770 GO:0044772 GO:0044843 GO:0045004 GO:0045005 GO:0046483 GO:0048046 GO:0048364 GO:0048519 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048598 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051302 GO:0051716 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071897 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:0099402 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1903047 GO:1905392 GO:1990234 GO:2000026 GO:2000028 GO:2000241 GO:2000242
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

96

Amino Acids

10.95

Weight (kDa)

5.52

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031180)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002028.1_g000013
rosa_samantha Rh5AG359000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 149
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 3 cut(s) 57, 127, 246
AluI AGCT 3 cut(s) 57, 127, 246
ApeKI GCWGC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 39
Asp700I GAANNNNTTC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 134
AvaII GGWCC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 255
BccI CCATC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 158
BisI GCNGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 82, 83
BmrFI CCNGG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 186
BseMI GCAATG 1 cut(s) 15
BseRI GAGGAG 2 cut(s) 269, 272
BseXI GCAGC 1 cut(s) 255
BshVI ATCGAT 1 cut(s) 186
BslFI GGGAC 1 cut(s) 94
BsmFI GGGAC 1 cut(s) 94
Bsp143I GATC 3 cut(s) 108, 132, 183
BspDI ATCGAT 1 cut(s) 186
BspLI GGNNCC 2 cut(s) 82, 83
BspQI GCTCTTC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 15
BssMI GATC 3 cut(s) 108, 132, 183
Bst2UI CCWGG 1 cut(s) 158
Bst6I CTCTTC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 27
BstKTI GATC 3 cut(s) 111, 135, 186
BstMBI GATC 3 cut(s) 108, 132, 183
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 156
BstSNI TACGTA 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 255
Bsu15I ATCGAT 1 cut(s) 186
BsuTUI ATCGAT 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 81
ClaI ATCGAT 1 cut(s) 186
CsiI ACCWGGT 1 cut(s) 156
CviJI RGCY 4 cut(s) 57, 127, 220, 246
CviKI_1 RGCY 4 cut(s) 57, 127, 220, 246
DdeI CTNAG 1 cut(s) 27
DpnI GATC 3 cut(s) 110, 134, 185
DpnII GATC 3 cut(s) 108, 132, 183
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco105I TACGTA 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 81
EcoRI GAATTC 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 156
FaiI YATR 3 cut(s) 60, 204, 239
FaqI GGGAC 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 244
GluI GCNGC 1 cut(s) 244
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 2 cut(s) 29, 226
HpyCH4IV ACGT 2 cut(s) 115, 249
HpyCH4V TGCA 2 cut(s) 4, 271
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 2 cut(s) 115, 249
KflI GGGWCCC 1 cut(s) 81
Kzo9I GATC 3 cut(s) 108, 132, 183
LguI GCTCTTC 1 cut(s) 47
LpnPI CCDG 5 cut(s) 33, 143, 161, 170, 200
Lsp1109I GCAGC 1 cut(s) 255
MabI ACCWGGT 1 cut(s) 156
MaeII ACGT 2 cut(s) 115, 249
MaeIII GTNAC 1 cut(s) 208
MalI GATC 3 cut(s) 110, 134, 185
MboI GATC 3 cut(s) 108, 132, 183
MboII GAAGA 3 cut(s) 44, 64, 142
MluCI AATT 3 cut(s) 39, 45, 192
MnlI CCTC 4 cut(s) 113, 165, 247, 250
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 3 cut(s) 48, 69, 96
MspR9I CCNGG 1 cut(s) 158
MvaI CCWGG 1 cut(s) 158
NdeII GATC 3 cut(s) 108, 132, 183
NlaIV GGNNCC 2 cut(s) 82, 83
PciSI GCTCTTC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 39
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 1 cut(s) 245
Ppu21I YACGTR 1 cut(s) 250
PpuMI RGGWCCY 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 82, 83
PspPI GGNCC 1 cut(s) 81
PspPPI RGGWCCY 1 cut(s) 81
PsyI GACNNNGTC 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 47
SaqAI TTAA 3 cut(s) 48, 69, 96
SatI GCNGC 1 cut(s) 244
Sau3AI GATC 3 cut(s) 108, 132, 183
Sau96I GGNCC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 158
SetI ASST 8 cut(s) 59, 103, 118, 124, 129, 162, 248, 252
SexAI ACCWGGT 1 cut(s) 156
SgeI CNNG 8 cut(s) 17, 32, 98, 160, 169, 170, 189, 227
SinI GGWCC 1 cut(s) 81
SnaBI TACGTA 1 cut(s) 250
Sse9I AATT 3 cut(s) 39, 45, 192
StyD4I CCNGG 1 cut(s) 156
TaiI ACGT 2 cut(s) 118, 252
TaqI TCGA 1 cut(s) 186
TasI AATT 3 cut(s) 39, 45, 192
Tru1I TTAA 3 cut(s) 48, 69, 96
Tru9I TTAA 3 cut(s) 48, 69, 96
TseI GCWGC 1 cut(s) 243
TspDTI ATGAA 2 cut(s) 204, 219
Tth111I GACNNNGTC 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 1 cut(s) 39
XmnI GAANNNNTTC 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.