Rh5AG359000

DNA polymerase epsilon catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0031180
Physical Location & Seq
Forward (+)
60364723 .. 60379234
14512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG359000.1

Sequence Viewer

Length: 477 bp
ATGTGGTCAATTGAATTTGAAGTGGATGGACCATACAAGGCAATGATTATTCCTGCTTCTAAGGAAGAAGGAATTCTAATTAAGAAGCGCTATGCTGTCTTTAATGATGATGGGACCCTTGCTGAACTTAAAGGTTTTGAGATCAAACGTAGAGGTGAGCTGAAGCTCATCAAAGTTTTCCAGAATCAAGGAAAGGATATAGCAGACAGTGAGTTGCTTGATTATATATCAGAATCAAGCACCAAGAGCAAGTCTTTGGCGGAATATGGAAGCCAAAAGTCATGTGCAGCAACCACTGCAAGGCGTCTTGCTGATTTTCTTGGTGATACAATGGTCAAAGATAAAGGACTACGTTGCCAGTATATAGTTGCATGTGAACCAAAGATGTTCAAGACAGCAATCCCAACTCAAAGAGACTGGAAACTAATTTTTGGCTTAGCCAAGATAGACAGTGACTTGCACTATACGCTCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
DNA_pol_B DNA_pol_B_exo1 DUF1744

Protein Analysis

158

Amino Acids

17.85

Weight (kDa)

8.34

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
POL2_thumb PF22634 62 - 134 4.4e-21 DNA polymerase epsilon catalytic subunit A, thumb domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031180)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002028.1_g000013
rosa_samantha Rh5AG359000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 260
AcsI RAATTY 2 cut(s) 14, 72
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 304
AfeI AGCGCT 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 300
AgsI TTSAA 3 cut(s) 14, 20, 391
AluBI AGCT 2 cut(s) 160, 166
AluI AGCT 2 cut(s) 160, 166
Alw26I GTCTC 1 cut(s) 408
Aor51HI AGCGCT 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 2 cut(s) 14, 72
Asp700I GAANNNNTTC 1 cut(s) 72
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 2 cut(s) 29, 114
AsuHPI GGTGA 2 cut(s) 167, 335
AvaII GGWCC 2 cut(s) 29, 114
BaeI ACNNNNGTAYC 2 cut(s) 318, 351
BbvI GCAGC 1 cut(s) 299
BccI CCATC 2 cut(s) 20, 104
BcoDI GTCTC 1 cut(s) 408
BfoI RGCGCY 1 cut(s) 91
BisI GCNGC 1 cut(s) 288
BlpI GCTNAGC 1 cut(s) 436
BlsI GCNGC 1 cut(s) 289
Bme18I GGWCC 2 cut(s) 29, 114
BmgT120I GGNCC 2 cut(s) 29, 114
BmiI GGNNCC 2 cut(s) 115, 116
Bpu1102I GCTNAGC 1 cut(s) 436
BsaHI GRCGYC 1 cut(s) 304
Bsc4I CCNNNNNNNGG 1 cut(s) 300
Bse1I ACTGG 2 cut(s) 358, 422
Bse3DI GCAATG 1 cut(s) 48
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 300
BseMI GCAATG 1 cut(s) 48
BseNI ACTGG 2 cut(s) 358, 422
BseXI GCAGC 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 306
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 300
BsmAI GTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 127
Bsp143I GATC 1 cut(s) 141
Bsp1720I GCTNAGC 1 cut(s) 436
BspACI CCGC 1 cut(s) 260
BspLI GGNNCC 2 cut(s) 115, 116
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 2 cut(s) 358, 422
BssMI GATC 1 cut(s) 141
BssNI GRCGYC 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 209, 452
BstACI GRCGYC 1 cut(s) 304
BstAPI GCANNNNNTGC 1 cut(s) 296
BstDEI CTNAG 2 cut(s) 60, 436
BstF5I GGATG 1 cut(s) 31
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 1 cut(s) 144
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 1 cut(s) 141
BstMWI GCNNNNNNNGC 3 cut(s) 246, 296, 466
BstNSI RCATGY 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 299
BtsCI GGATG 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 3 cut(s) 214, 294, 457
CfoI GCGC 1 cut(s) 90
Cfr13I GGNCC 2 cut(s) 29, 114
CseI GACGC 1 cut(s) 293
CviAII CATG 2 cut(s) 282, 372
CviJI RGCY 5 cut(s) 160, 166, 273, 435, 440
CviKI_1 RGCY 5 cut(s) 160, 166, 273, 435, 440
DdeI CTNAG 2 cut(s) 60, 436
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 29, 114
Eco47III AGCGCT 1 cut(s) 89
Eco57I CTGAAG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 114
EcoRI GAATTC 1 cut(s) 72
FaeI CATG 2 cut(s) 285, 375
FaqI GGGAC 1 cut(s) 127
FatI CATG 2 cut(s) 281, 371
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 1 cut(s) 288
GlaI GCGC 1 cut(s) 89
GluI GCNGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 91
HgaI GACGC 1 cut(s) 293
HhaI GCGC 1 cut(s) 90
Hin1I GRCGYC 1 cut(s) 304
Hin1II CATG 2 cut(s) 285, 375
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HinfI GANTC 2 cut(s) 184, 233
HphI GGTGA 2 cut(s) 167, 335
Hpy166II GTNNAC 1 cut(s) 377
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 2 cut(s) 181, 391
Hpy8I GTNNAC 1 cut(s) 377
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 2 cut(s) 209, 452
HpyCH4IV ACGT 2 cut(s) 148, 352
HpyCH4V TGCA 4 cut(s) 287, 299, 371, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 246, 296, 466
HpyF3I CTNAG 2 cut(s) 60, 436
HpySE526I ACGT 2 cut(s) 148, 352
Hsp92I GRCGYC 1 cut(s) 304
Hsp92II CATG 2 cut(s) 285, 375
HspAI GCGC 1 cut(s) 88
KflI GGGWCCC 1 cut(s) 114
Kzo9I GATC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 474
LpnPI CCDG 4 cut(s) 66, 194, 371, 403
Lsp1109I GCAGC 1 cut(s) 299
MaeII ACGT 2 cut(s) 148, 352
MaeIII GTNAC 1 cut(s) 452
MalI GATC 1 cut(s) 143
MboI GATC 1 cut(s) 141
MboII GAAGA 1 cut(s) 77
MfeI CAATTG 1 cut(s) 9
MluCI AATT 5 cut(s) 9, 14, 72, 78, 426
MnlI CCTC 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 72
MseI TTAA 3 cut(s) 81, 102, 129
MunI CAATTG 1 cut(s) 9
MwoI GCNNNNNNNGC 3 cut(s) 246, 296, 466
NdeII GATC 1 cut(s) 141
NlaIII CATG 2 cut(s) 285, 375
NlaIV GGNNCC 2 cut(s) 115, 116
NmuCI GTSAC 1 cut(s) 452
NspI RCATGY 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 2 cut(s) 184, 233
PkrI GCNGC 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 114
Psp5II RGGWCCY 1 cut(s) 114
PspN4I GGNNCC 2 cut(s) 115, 116
PspPI GGNCC 2 cut(s) 29, 114
PspPPI RGGWCCY 1 cut(s) 114
SaqAI TTAA 3 cut(s) 81, 102, 129
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 1 cut(s) 141
Sau96I GGNCC 2 cut(s) 29, 114
SetI ASST 6 cut(s) 136, 151, 157, 162, 168, 355
SinI GGWCC 2 cut(s) 29, 114
Sse9I AATT 5 cut(s) 9, 14, 72, 78, 426
SsiI CCGC 1 cut(s) 260
TaaI ACNGT 2 cut(s) 209, 452
TaiI ACGT 2 cut(s) 151, 355
TasI AATT 5 cut(s) 9, 14, 72, 78, 426
TfiI GAWTC 2 cut(s) 184, 233
Tru1I TTAA 3 cut(s) 81, 102, 129
Tru9I TTAA 3 cut(s) 81, 102, 129
TscAI CASTG 3 cut(s) 214, 301, 457
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 452
TspRI CASTG 3 cut(s) 214, 301, 457
VpaK11BI GGWCC 2 cut(s) 29, 114
XapI RAATTY 2 cut(s) 14, 72
XceI RCATGY 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.