Rmu_sc0002510.1_g000010

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002510.1
OG0025453
Physical Location & Seq
Reverse (-)
32228 .. 32885
658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002510.1_g000010.1.cds

Sequence Viewer

Length: 366 bp
atgatgtcgacaagctatcgacagcctgttgacatgctgtcgacatggatgccgagttcttcagacgacagttctctaaatcatatttcatcatatggcagcgagatgtacgacagtcagctccgtatcagacgacagtttttagccgcaggagcagtgaaagtgagtgatgtgtcatttactggtttccatggaacttcagcggttgagcaagcgataacattggattgctctaatttgggttgcactaacattataatggatcatgtcaacataacctcagctgtgcctggaagctactccgagatttatgcaacaatgccaaaggaaaatcaagccttactacacctgatgtttcttgcctag

Protein Analysis

121

Amino Acids

13.28

Weight (kDa)

5.06

Isoelectric Point (pI)

64.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025453)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002510.1_g000010 Rmu_ssc0000125.1_g000024
rosa_rugosa Rorug05G0595200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AccI GTMKAC 2 cut(s) 8, 41
AciI CCGC 2 cut(s) 147, 203
AclWI GGATC 1 cut(s) 270
AcuI CTGAAG 2 cut(s) 45, 183
AfaI GTAC 1 cut(s) 110
AhdI GACNNNNNGTC 1 cut(s) 37
AjnI CCWGG 1 cut(s) 289
AloI GAACNNNNNNTCC 2 cut(s) 40, 72
AluBI AGCT 4 cut(s) 15, 121, 284, 297
AluI AGCT 4 cut(s) 15, 121, 284, 297
AlwI GGATC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 99
BbvCI CCTCAGC 1 cut(s) 280
BbvI GCAGC 1 cut(s) 111
BcgI CGANNNNNNTGC 4 cut(s) 31, 65, 293, 327
BciT130I CCWGG 1 cut(s) 291
BfaI CTAG 1 cut(s) 364
BisI GCNGC 2 cut(s) 100, 147
BlsI GCNGC 2 cut(s) 101, 148
Bme1390I CCNGG 1 cut(s) 291
BmeRI GACNNNNNGTC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 54
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 262
Bsp19I CCATGG 1 cut(s) 190
BspACI CCGC 2 cut(s) 147, 203
BspCNI CTCAG 1 cut(s) 293
BspPI GGATC 1 cut(s) 270
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 71, 116, 138
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 280
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 1 cut(s) 54
BstKTI GATC 1 cut(s) 265
BstMBI GATC 1 cut(s) 262
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 37
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 111
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 213
Csp6I GTAC 1 cut(s) 109
CviAII CATG 4 cut(s) 34, 45, 191, 266
CviJI RGCY 7 cut(s) 15, 25, 121, 146, 284, 297, 338
CviKI_1 RGCY 7 cut(s) 15, 25, 121, 146, 284, 297, 338
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 1 cut(s) 280
DpnI GATC 1 cut(s) 264
DpnII GATC 1 cut(s) 262
DriI GACNNNNNGTC 1 cut(s) 37
Eam1105I GACNNNNNGTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 45, 183
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 4 cut(s) 37, 48, 194, 269
FatI CATG 4 cut(s) 33, 44, 190, 265
FauNDI CATATG 1 cut(s) 94
FblI GTMKAC 2 cut(s) 8, 41
Fnu4HI GCNGC 2 cut(s) 100, 147
FokI GGATG 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 100, 147
FspBI CTAG 1 cut(s) 364
GluI GCNGC 2 cut(s) 100, 147
Hin1II CATG 4 cut(s) 37, 48, 194, 269
HincII GTYRAC 4 cut(s) 9, 31, 42, 271
HindII GTYRAC 4 cut(s) 9, 31, 42, 271
Hpy166II GTNNAC 4 cut(s) 9, 31, 42, 271
Hpy188I TCNGA 3 cut(s) 64, 131, 304
Hpy8I GTNNAC 4 cut(s) 9, 31, 42, 271
HpyCH4III ACNGT 3 cut(s) 71, 116, 138
HpyCH4V TGCA 2 cut(s) 246, 314
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
HpyF3I CTNAG 1 cut(s) 280
Hsp92II CATG 4 cut(s) 37, 48, 194, 269
Kzo9I GATC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 126, 152
LpnPI CCDG 6 cut(s) 39, 135, 168, 276, 303, 362
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 1 cut(s) 39
MaeI CTAG 1 cut(s) 364
MalI GATC 1 cut(s) 264
MboI GATC 1 cut(s) 262
MboII GAAGA 1 cut(s) 51
MluCI AATT 1 cut(s) 235
MnlI CCTC 1 cut(s) 289
MslI CAYNNNNRTG 1 cut(s) 257
MspA1I CMGCKG 2 cut(s) 203, 284
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 152
NcoI CCATGG 1 cut(s) 190
NdeI CATATG 1 cut(s) 94
NdeII GATC 1 cut(s) 262
NlaIII CATG 4 cut(s) 37, 48, 194, 269
NmeAIII GCCGAG 1 cut(s) 78
NspI RCATGY 1 cut(s) 37
PkrI GCNGC 2 cut(s) 101, 148
PsiI TTATAA 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 257
SalI GTCGAC 2 cut(s) 7, 40
SatI GCNGC 2 cut(s) 100, 147
Sau3AI GATC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 291
SetI ASST 6 cut(s) 17, 123, 281, 286, 299, 351
SfaNI GCATC 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 257
Sse9I AATT 1 cut(s) 235
SsiI CCGC 2 cut(s) 147, 203
SspMI CTAG 1 cut(s) 364
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 3 cut(s) 71, 116, 138
TaqI TCGA 3 cut(s) 8, 19, 41
TasI AATT 1 cut(s) 235
TauI GCSGC 1 cut(s) 149
TscAI CASTG 1 cut(s) 162
TseI GCWGC 1 cut(s) 99
TspDTI ATGAA 1 cut(s) 78
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 1 cut(s) 162
XceI RCATGY 1 cut(s) 37
XmiI GTMKAC 2 cut(s) 8, 41
XspI CTAG 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.