Rmu_sc0002587.1_g000046

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002587.1
OG0028968
Physical Location & Seq
Forward (+)
143954 .. 145606
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002587.1_g000046.1.cds

Sequence Viewer

Length: 930 bp
atggggccagccaacaatctgggtcgcatttggttttgctttgtttgcaagaagcacgagtctggtcataggtgtggattaagttttggggatggtgatttactttgtatgagattgattatttgcatttgggaaagttgttgttccttgtgctttgacggctctggaccactcgctgtcggaagttatggaggccggattcgtcgacggccggtgagcacggtggtggtgtcggtgtttaccggtggagctccgaagagaggagtggaagaagagagagtagacttcagcaagggtagaagagcagagcactttgctgaagcgttcgatgaacatgaagagaagcttggggaaggtaataaggaggtggacggctggagagatgctttaacaaaagtagccaatctcgctggctggacttcaaaggattataggtatgaaacagagcttatcaaagagattgtgcaagcacagtggagcaaagtgcatcacagtctcacaatatttggttcctcagagaagttagttggaatggacactaagctggaggaaatagaagttcttttagatcaagaagcaaatgatgttcgctttctaggggtatgggggatgggtgggttgggtaagagtaagacgacccttgttagactagtttatgagaaaatctctcatcaatttgaagtttgtatctttcttgctaatgtcagagaggtttctgcaaaccacggtctagcttatctacaaaagcagattctttccgaaatcttgaaggaagaaaatgtacaagtatgggatgtttatagtggaatcgctatgactaagagatgtttatgtaataaagcagttcttctaattcttgacgatgtggataaagtagagcaacttgaaaccttggtgggagaaaaagtctgctttggtttgaggctttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

309

Amino Acids

34.64

Weight (kDa)

5.97

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028968)

Species Orthologous Gene IDs
prunus_persica Prupe.8G056600_v2.0.a1
rosa_multiflora Rmu_sc0002587.1_g000046

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 205, 282
AcoI YGGCCR 1 cut(s) 209
AcuI CTGAAG 2 cut(s) 271, 339
AfaI GTAC 1 cut(s) 783
AfiI CCNNNNNNNGG 1 cut(s) 260
AgeI ACCGGT 1 cut(s) 242
AgsI TTSAA 4 cut(s) 423, 680, 769, 887
AhlI ACTAGT 1 cut(s) 649
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 5 cut(s) 251, 346, 448, 544, 734
AluI AGCT 5 cut(s) 251, 346, 448, 544, 734
Alw21I GWGCWC 3 cut(s) 221, 253, 312
Alw26I GTCTC 1 cut(s) 500
AoxI GGCC 3 cut(s) 5, 193, 209
AsiGI ACCGGT 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 5, 167
AsuHPI GGTGA 2 cut(s) 107, 226
AvaII GGWCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 253
BarI GAAGNNNNNNTAC 2 cut(s) 765, 797
BauI CACGAG 1 cut(s) 56
Bbv12I GWGCWC 3 cut(s) 221, 253, 312
BccI CCATC 2 cut(s) 86, 604
BceAI ACGGC 3 cut(s) 175, 224, 388
BcoDI GTCTC 1 cut(s) 500
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 3 cut(s) 596, 650, 731
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 5, 167
BmiI GGNNCC 2 cut(s) 6, 511
BmsI GCATC 2 cut(s) 373, 496
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 2 cut(s) 397, 566
BsaJI CCNNGG 2 cut(s) 724, 891
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse118I RCCGGY 2 cut(s) 211, 242
BseDI CCNNGG 2 cut(s) 724, 891
BseGI GGATG 3 cut(s) 97, 615, 799
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMII CTCAG 1 cut(s) 528
BseRI GAGGAG 1 cut(s) 276
BseX3I CGGCCG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 3 cut(s) 7, 195, 211
BshTI ACCGGT 1 cut(s) 242
BsiEI CGRYCG 1 cut(s) 212
BsiHKAI GWGCWC 3 cut(s) 221, 253, 312
BsiSI CCGG 3 cut(s) 196, 212, 243
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 500
BsnI GGCC 3 cut(s) 7, 195, 211
Bsp1286I GDGCHC 3 cut(s) 221, 253, 312
Bsp1407I TGTACA 1 cut(s) 781
Bsp143I GATC 1 cut(s) 568
BspANI GGCC 3 cut(s) 7, 195, 211
BspCNI CTCAG 1 cut(s) 527
BspLI GGNNCC 2 cut(s) 6, 511
BspQI GCTCTTC 1 cut(s) 295
BsrFI RCCGGY 2 cut(s) 211, 242
BsrGI TGTACA 1 cut(s) 781
BssAI RCCGGY 2 cut(s) 211, 242
BssECI CCNNGG 2 cut(s) 724, 891
BssMI GATC 1 cut(s) 568
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 891
Bst2BI CACGAG 1 cut(s) 56
Bst4CI ACNGT 4 cut(s) 223, 474, 494, 728
Bst6I CTCTTC 4 cut(s) 251, 267, 295, 333
BstAUI TGTACA 1 cut(s) 781
BstC8I GCNNGC 3 cut(s) 9, 412, 468
BstDEI CTNAG 3 cut(s) 514, 540, 819
BstDSI CCRYGG 1 cut(s) 724
BstF5I GGATG 3 cut(s) 97, 615, 799
BstKTI GATC 1 cut(s) 571
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 1 cut(s) 568
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 3 cut(s) 45, 159, 407
BstXI CCANNNNNNTGG 1 cut(s) 19
BstZI CGGCCG 1 cut(s) 209
BsuRI GGCC 3 cut(s) 7, 195, 211
BtgI CCRYGG 1 cut(s) 724
BtsCI GGATG 3 cut(s) 97, 615, 799
BtsIMutI CAGTG 1 cut(s) 479
Cac8I GCNNGC 3 cut(s) 9, 412, 468
Cfr10I RCCGGY 2 cut(s) 211, 242
Cfr13I GGNCC 2 cut(s) 5, 167
Csp6I GTAC 1 cut(s) 782
CspAI ACCGGT 1 cut(s) 242
CspCI CAANNNNNGTGG 2 cut(s) 455, 490
CviAII CATG 1 cut(s) 335
CviQI GTAC 1 cut(s) 782
DdeI CTNAG 3 cut(s) 514, 540, 819
DpnI GATC 1 cut(s) 570
DpnII GATC 1 cut(s) 568
EaeI YGGCCR 1 cut(s) 209
EagI CGGCCG 1 cut(s) 209
Eam1104I CTCTTC 4 cut(s) 251, 267, 295, 333
EarI CTCTTC 4 cut(s) 251, 267, 295, 333
Ecl136II GAGCTC 1 cut(s) 251
EclXI CGGCCG 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 891
Eco24I GRGCYC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 167
Eco52I CGGCCG 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 2 cut(s) 271, 339
EcoICRI GAGCTC 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 891
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 891
FaeI CATG 1 cut(s) 338
FalI AAGNNNNNCTT 4 cut(s) 330, 362, 831, 863
FatI CATG 1 cut(s) 334
FblI GTMKAC 2 cut(s) 205, 282
FokI GGATG 3 cut(s) 104, 622, 806
FriOI GRGCYC 1 cut(s) 253
FspBI CTAG 3 cut(s) 596, 650, 731
GsuI CTGGAG 2 cut(s) 397, 566
HaeIII GGCC 3 cut(s) 7, 195, 211
HapII CCGG 3 cut(s) 196, 212, 243
Hin1II CATG 1 cut(s) 338
HincII GTYRAC 1 cut(s) 206
HindII GTYRAC 1 cut(s) 206
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 4 cut(s) 59, 199, 751, 807
HpaII CCGG 3 cut(s) 196, 212, 243
HphI GGTGA 2 cut(s) 107, 226
Hpy166II GTNNAC 4 cut(s) 206, 240, 283, 370
Hpy188I TCNGA 5 cut(s) 182, 255, 517, 707, 760
Hpy188III TCNNGA 4 cut(s) 165, 572, 766, 857
Hpy8I GTNNAC 4 cut(s) 206, 240, 283, 370
Hpy99I CGWCG 2 cut(s) 207, 210
HpyAV CCTTC 2 cut(s) 347, 763
HpyCH4III ACNGT 4 cut(s) 223, 474, 494, 728
HpyCH4V TGCA 5 cut(s) 48, 126, 466, 487, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 159, 407
HpyF3I CTNAG 3 cut(s) 514, 540, 819
Hsp92II CATG 1 cut(s) 338
Kzo9I GATC 1 cut(s) 568
LguI GCTCTTC 1 cut(s) 295
LmnI GCTCC 3 cut(s) 248, 256, 477
LweI GCATC 2 cut(s) 373, 496
MaeI CTAG 3 cut(s) 596, 650, 731
MalI GATC 1 cut(s) 570
MboI GATC 1 cut(s) 568
MboII GAAGA 7 cut(s) 268, 281, 284, 312, 350, 785, 839
MhlI GDGCHC 3 cut(s) 221, 253, 312
MluCI AATT 2 cut(s) 674, 852
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 2 cut(s) 160, 508
MnlI CCTC 7 cut(s) 185, 254, 358, 523, 541, 703, 915
MseI TTAA 2 cut(s) 80, 389
MslI CAYNNNNRTG 2 cut(s) 72, 224
MspI CCGG 3 cut(s) 196, 212, 243
MwoI GCNNNNNNNGC 3 cut(s) 45, 159, 407
NdeII GATC 1 cut(s) 568
NlaIII CATG 1 cut(s) 338
NlaIV GGNNCC 2 cut(s) 6, 511
OliI CACNNNNGTG 1 cut(s) 224
PciSI GCTCTTC 1 cut(s) 295
PfeI GAWTC 3 cut(s) 199, 751, 807
PinAI ACCGGT 1 cut(s) 242
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 6, 511
PspPI GGNCC 2 cut(s) 5, 167
RsaI GTAC 1 cut(s) 783
RsaNI GTAC 1 cut(s) 782
RseI CAYNNNNRTG 2 cut(s) 72, 224
SacI GAGCTC 1 cut(s) 253
SalI GTCGAC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 295
SaqAI TTAA 2 cut(s) 80, 389
Sau3AI GATC 1 cut(s) 568
Sau96I GGNCC 2 cut(s) 5, 167
SchI GAGTC 1 cut(s) 68
SduI GDGCHC 3 cut(s) 221, 253, 312
SfaNI GCATC 2 cut(s) 373, 496
SgrDI CGTCGACG 1 cut(s) 204
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 2 cut(s) 72, 224
SpeI ACTAGT 1 cut(s) 649
Sse9I AATT 2 cut(s) 674, 852
SspI AATATT 1 cut(s) 504
SspMI CTAG 3 cut(s) 596, 650, 731
SstI GAGCTC 1 cut(s) 253
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 4 cut(s) 223, 474, 494, 728
TaqI TCGA 2 cut(s) 205, 327
TasI AATT 2 cut(s) 674, 852
TatI WGTACW 1 cut(s) 781
TfiI GAWTC 3 cut(s) 199, 751, 807
Tru1I TTAA 2 cut(s) 80, 389
Tru9I TTAA 2 cut(s) 80, 389
TscAI CASTG 1 cut(s) 479
TspDTI ATGAA 3 cut(s) 345, 351, 453
TspRI CASTG 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 167
XmiI GTMKAC 2 cut(s) 205, 282
XspI CTAG 3 cut(s) 596, 650, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.