Rmu_sc0003076.1_g000033

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003076.1
OG0031241
Physical Location & Seq
Reverse (-)
162504 .. 166404
3901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003076.1_g000033.1.cds

Sequence Viewer

Length: 1056 bp
atgctcggtaaagaagggtggaggttaatgtccaatcctcattctttgattactagattatacaaggccaagtactttcccaattgctccttctgggaggttgaattgggagactctccatcattctcttggagaagcattctaagtgaaaaaccagtgtttaaagctggggaactgtggaagagactttggaaggcaaaagttccagggaaagttcagatttgcgtgtggagaacatgctccaatttgcttcctgcaagtgctaaacttagtactaaaggatatgagggtgacctccattgtctactgtgttcccatccttacaaagacacttcccatgtcttttgtaagtgccctactgcagctgctattctagctgcacccccgtttaattttggacacaatctgttgcctcatattgagtctaaagaatggatgcttgaaaatgcattgcatttaaaggaggtggaacttagggatataaaagaagttttaaacttggtggcaacagtacaagttagcaagattgtcatcgagacagactgcttggatgctgtctccaacattgccaacacgcaatctacttatgtacatgatgaaggcataattgatgatattcgtcaagaactccatcagaggactgaaattacattgcagcatacaccaaggacggtggtccagcacctgtgtcgcctcttgtcgtgcgatgtcgtggtgccgctgccggacgggtggttgtttctaaggcttgctttggattacaaggagaggtttgcacaacggctaacagatgtggttgacggccttgttcctggcttggttatctctgttgcattaatgctcgtagtaaccgaaaaagtcgcgccacaaggaaaagacgaaagaaaaacaagaagaacaaacgacgtgaaaagctatgaaatggcacggtatccatttgatttgacactctacttcttctgcttctctccggctcagaataatcatcgtcgtcgtcgtcgtcgtcaccttcgctcttcaatttccgaggacatggagctgaaaccaattctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

351

Amino Acids

40.34

Weight (kDa)

8.79

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031241)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003076.1_g000033
rosa_rugosa Rorug03G0202200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 715
AccI GTMKAC 1 cut(s) 304
AccII CGCG 1 cut(s) 863
AciI CCGC 1 cut(s) 719
AfaI GTAC 4 cut(s) 74, 274, 513, 591
AgsI TTSAA 3 cut(s) 104, 443, 1020
AjiI CACGTC 1 cut(s) 907
AjnI CCWGG 2 cut(s) 205, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 5 cut(s) 167, 365, 377, 915, 1039
AluI AGCT 5 cut(s) 167, 365, 377, 915, 1039
Alw26I GTCTC 4 cut(s) 105, 178, 530, 562
AlwNI CAGNNNCTG 1 cut(s) 685
AoxI GGCC 2 cut(s) 66, 802
ApeKI GCWGC 5 cut(s) 362, 365, 377, 655, 721
AseI ATTAAT 1 cut(s) 836
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 2 cut(s) 302, 998
AvaII GGWCC 1 cut(s) 676
BaeGI GKGCMC 1 cut(s) 356
BanI GGYRCC 1 cut(s) 715
BbvI GCAGC 5 cut(s) 352, 364, 374, 667, 708
BccI CCATC 3 cut(s) 127, 324, 639
BceAI ACGGC 2 cut(s) 797, 817
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 2 cut(s) 207, 813
BciVI GTATCC 1 cut(s) 942
BcoDI GTCTC 4 cut(s) 105, 178, 530, 562
BfaI CTAG 2 cut(s) 54, 374
BfmI CTRYAG 1 cut(s) 360
BfuI GTATCC 1 cut(s) 942
BisI GCNGC 6 cut(s) 363, 366, 378, 656, 719, 722
BlsI GCNGC 6 cut(s) 364, 367, 379, 657, 720, 723
BmcAI AGTACT 2 cut(s) 74, 274
Bme1390I CCNGG 2 cut(s) 207, 813
Bme18I GGWCC 1 cut(s) 676
BmgBI CACGTC 1 cut(s) 907
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 1 cut(s) 717
BmrFI CCNGG 2 cut(s) 207, 813
BmsI GCATC 2 cut(s) 426, 541
BoxI GACNNNNGTC 1 cut(s) 674
BsaBI GATNNNNATC 1 cut(s) 530
BsaJI CCNNGG 3 cut(s) 206, 665, 1026
Bse1I ACTGG 1 cut(s) 155
Bse3DI GCAATG 3 cut(s) 449, 564, 650
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 2 cut(s) 207, 813
BseDI CCNNGG 3 cut(s) 206, 665, 1026
BseGI GGATG 3 cut(s) 316, 441, 556
BseJI GATNNNNATC 1 cut(s) 530
BseMI GCAATG 3 cut(s) 449, 564, 650
BseMII CTCAG 1 cut(s) 989
BseNI ACTGG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 5 cut(s) 352, 364, 374, 667, 708
BseYI CCCAGC 1 cut(s) 167
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 863
BshFI GGCC 2 cut(s) 68, 804
BshNI GGYRCC 1 cut(s) 715
BsiSI CCGG 2 cut(s) 725, 971
BsmAI GTCTC 4 cut(s) 105, 178, 530, 562
BsmI GAATGC 1 cut(s) 138
BsnI GGCC 2 cut(s) 68, 804
Bsp1286I GDGCHC 1 cut(s) 356
Bsp1407I TGTACA 1 cut(s) 589
BspACI CCGC 1 cut(s) 719
BspANI GGCC 2 cut(s) 68, 804
BspCNI CTCAG 1 cut(s) 988
BspFNI CGCG 1 cut(s) 863
BspLI GGNNCC 1 cut(s) 717
BspMAI CTGCAG 1 cut(s) 364
BspQI GCTCTTC 1 cut(s) 1021
BspT107I GGYRCC 1 cut(s) 715
BsrDI GCAATG 3 cut(s) 449, 564, 650
BsrGI TGTACA 1 cut(s) 589
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 3 cut(s) 206, 665, 1026
BssT1I CCWWGG 1 cut(s) 665
Bst2UI CCWGG 2 cut(s) 207, 813
Bst4CI ACNGT 5 cut(s) 177, 309, 511, 673, 930
Bst6I CTCTTC 2 cut(s) 176, 1021
BstAUI TGTACA 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 5 cut(s) 143, 269, 473, 743, 975
BstEII GGTNACC 1 cut(s) 290
BstF5I GGATG 3 cut(s) 316, 441, 556
BstFNI CGCG 1 cut(s) 863
BstHHI GCGC 1 cut(s) 865
BstMAI GTCTC 4 cut(s) 105, 178, 530, 562
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 2 cut(s) 207, 813
BstNSI RCATGY 1 cut(s) 240
BstPAI GACNNNNGTC 1 cut(s) 674
BstPI GGTNACC 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 205, 811
BstSFI CTRYAG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 356
BstUI CGCG 1 cut(s) 863
BstV1I GCAGC 5 cut(s) 352, 364, 374, 667, 708
BsuI GTATCC 1 cut(s) 942
BsuRI GGCC 2 cut(s) 68, 804
BtgZI GCGATG 1 cut(s) 720
BtrI CACGTC 1 cut(s) 907
BtsCI GGATG 3 cut(s) 316, 441, 556
BtsIMutI CAGTG 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 750
CaiI CAGNNNCTG 1 cut(s) 685
CfoI GCGC 1 cut(s) 865
Cfr13I GGNCC 1 cut(s) 676
Csp6I GTAC 4 cut(s) 73, 273, 512, 590
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviAII CATG 4 cut(s) 237, 338, 593, 1033
CviQI GTAC 4 cut(s) 73, 273, 512, 590
DdeI CTNAG 5 cut(s) 143, 269, 473, 743, 975
DraI TTTAAA 3 cut(s) 163, 459, 495
Eam1104I CTCTTC 2 cut(s) 176, 1021
EarI CTCTTC 2 cut(s) 176, 1021
Eco130I CCWWGG 1 cut(s) 665
Eco47I GGWCC 1 cut(s) 676
Eco91I GGTNACC 1 cut(s) 290
EcoO65I GGTNACC 1 cut(s) 290
EcoRII CCWGG 2 cut(s) 205, 811
EcoT14I CCWWGG 1 cut(s) 665
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 665
FaeI CATG 4 cut(s) 240, 341, 596, 1036
FalI AAGNNNNNCTT 2 cut(s) 736, 768
FatI CATG 4 cut(s) 236, 337, 592, 1032
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 6 cut(s) 363, 366, 378, 656, 719, 722
FokI GGATG 3 cut(s) 303, 448, 563
Fsp4HI GCNGC 6 cut(s) 363, 366, 378, 656, 719, 722
FspBI CTAG 2 cut(s) 54, 374
GlaI GCGC 1 cut(s) 864
GluI GCNGC 6 cut(s) 363, 366, 378, 656, 719, 722
GsaI CCCAGC 1 cut(s) 171
HaeIII GGCC 2 cut(s) 68, 804
HapII CCGG 2 cut(s) 725, 971
HhaI GCGC 1 cut(s) 865
Hin1II CATG 4 cut(s) 240, 341, 596, 1036
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HinfI GANTC 2 cut(s) 113, 422
HpaII CCGG 2 cut(s) 725, 971
HphI GGTGA 2 cut(s) 302, 998
Hpy166II GTNNAC 2 cut(s) 305, 799
Hpy188I TCNGA 5 cut(s) 219, 636, 978, 1027, 1055
Hpy188III TCNNGA 2 cut(s) 535, 623
Hpy8I GTNNAC 2 cut(s) 305, 799
Hpy99I CGWCG 6 cut(s) 908, 993, 996, 999, 1002, 1005
HpyAV CCTTC 5 cut(s) 8, 100, 187, 593, 1019
HpyCH4III ACNGT 5 cut(s) 177, 309, 511, 673, 930
HpyCH4IV ACGT 1 cut(s) 906
HpyCH4V TGCA 8 cut(s) 257, 362, 380, 449, 454, 655, 776, 833
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 5 cut(s) 143, 269, 473, 743, 975
HpySE526I ACGT 1 cut(s) 906
Hsp92II CATG 4 cut(s) 240, 341, 596, 1036
HspAI GCGC 1 cut(s) 863
LguI GCTCTTC 1 cut(s) 1021
LmnI GCTCC 3 cut(s) 92, 245, 1036
Lsp1109I GCAGC 5 cut(s) 352, 364, 374, 667, 708
LweI GCATC 2 cut(s) 426, 541
MaeI CTAG 2 cut(s) 54, 374
MaeII ACGT 1 cut(s) 906
MaeIII GTNAC 3 cut(s) 290, 847, 1004
MboII GAAGA 4 cut(s) 193, 906, 949, 1008
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 8 cut(s) 82, 104, 244, 391, 606, 645, 1020, 1047
MlyI GAGTC 2 cut(s) 107, 431
MmeI TCCRAC 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 451
MseI TTAA 6 cut(s) 26, 162, 390, 458, 494, 836
MspA1I CMGCKG 2 cut(s) 365, 721
MspI CCGG 2 cut(s) 725, 971
MspR9I CCNGG 2 cut(s) 207, 813
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 138
MvaI CCWGG 2 cut(s) 207, 813
MvnI CGCG 1 cut(s) 863
MwoI GCNNNNNNNGC 1 cut(s) 374
NlaIII CATG 4 cut(s) 240, 341, 596, 1036
NlaIV GGNNCC 1 cut(s) 717
NmuCI GTSAC 2 cut(s) 290, 1004
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 1021
PcsI WCGNNNNNNNCGW 4 cut(s) 849, 994, 997, 1000
PctI GAATGC 1 cut(s) 138
PkrI GCNGC 6 cut(s) 364, 367, 379, 657, 720, 723
PleI GAGTC 2 cut(s) 107, 430
PpsI GAGTC 2 cut(s) 107, 430
PshAI GACNNNNGTC 1 cut(s) 674
PshBI ATTAAT 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 205, 811
PspEI GGTNACC 1 cut(s) 290
PspFI CCCAGC 1 cut(s) 167
PspGI CCWGG 2 cut(s) 205, 811
PspN4I GGNNCC 1 cut(s) 717
PspPI GGNCC 1 cut(s) 676
PstI CTGCAG 1 cut(s) 364
PstNI CAGNNNCTG 1 cut(s) 685
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 4 cut(s) 74, 274, 513, 591
RsaNI GTAC 4 cut(s) 73, 273, 512, 590
SapI GCTCTTC 1 cut(s) 1021
SaqAI TTAA 6 cut(s) 26, 162, 390, 458, 494, 836
SatI GCNGC 6 cut(s) 363, 366, 378, 656, 719, 722
Sau96I GGNCC 1 cut(s) 676
ScaI AGTACT 2 cut(s) 74, 274
SchI GAGTC 2 cut(s) 107, 431
ScrFI CCNGG 2 cut(s) 207, 813
SduI GDGCHC 1 cut(s) 356
SfaNI GCATC 2 cut(s) 426, 541
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 1 cut(s) 676
Sse9I AATT 8 cut(s) 82, 104, 244, 391, 606, 645, 1020, 1047
SsiI CCGC 1 cut(s) 719
SspMI CTAG 2 cut(s) 54, 374
StyD4I CCNGG 2 cut(s) 205, 811
StyI CCWWGG 1 cut(s) 665
TaaI ACNGT 5 cut(s) 177, 309, 511, 673, 930
TaiI ACGT 1 cut(s) 909
TaqI TCGA 1 cut(s) 534
TasI AATT 8 cut(s) 82, 104, 244, 391, 606, 645, 1020, 1047
TatI WGTACW 4 cut(s) 72, 272, 511, 589
TauI GCSGC 1 cut(s) 721
Tru1I TTAA 6 cut(s) 26, 162, 390, 458, 494, 836
Tru9I TTAA 6 cut(s) 26, 162, 390, 458, 494, 836
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 2 cut(s) 290, 1004
TseI GCWGC 5 cut(s) 362, 365, 377, 655, 721
Tsp45I GTSAC 2 cut(s) 290, 1004
TspDTI ATGAA 2 cut(s) 612, 933
TspRI CASTG 1 cut(s) 162
VpaK11BI GGWCC 1 cut(s) 676
VspI ATTAAT 1 cut(s) 836
XceI RCATGY 1 cut(s) 240
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 304
XspI CTAG 2 cut(s) 54, 374
ZrmI AGTACT 2 cut(s) 74, 274
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.