Rmu_sc0003318.1_g000003
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003318.1
OG0031114
Physical Location & Seq
Reverse (-)
11846 .. 15831
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003318.1_g000003.1.cds

Sequence Viewer

Length: 1047 bp
atggctctaaaagctgcaatcgaactcaatgtcttcaatatcatagccaattcaggacctggagctcatgtcacatcaaaagaaattgtatctcaaatctcaaccacgaatccaaatgcagcagctgagaatttgaagcgaatattaagagtcctaagtgtgcattctttgttatctgtatctcaaaggccaagctcaagtggtaagactgtactagaagagacttatggtttaacaaaggatactctttgcctagttccaaatgaagatggagtttccttagctccatttattatgttaacttctaatatgaccactgtgaaaagcttgtccatgcttaaagaaacagtgcttgaacctgggagctgtccttttaacaaggctcatggtgtgtccgcatacgaaggttttgaagaggtagaacagttgatggatgttggaggaggcaatggaactacaattgccaaagtagtctctcggtatccacacattcatgggattaacttcgatttacctaatgttgtcgctcaaggaattgctaaataccaagcagaggtcggttcggcagtggcttggggaaaaatggatcggtttagtaccgaatccagccctagattatttagtcattacattgcgagtcccattttaccaagtgatctcttaatgagtagaactaaactaatacccaaacctgagcaaagggagcaaaagcccaggccctcttctgccaagtcgtcgccgccgacgacgactctggcggtctgccgttccaacaatccatcaagtctgggtctacaagtcactaccccgatctgtctttcccttatgtacaccggaaggagaagctctgctacccgaacaaccctcccctccgacactacttctcaccccgacgccgcttcaatcagcaaaccgacttgtgctgccgatatgcccgccgtgcttgacttcgatcctcctccgctgcaagacgttaatcacttgaccacagatcccgatctgcactcactcgcactcgacccggcgtcttccaccaaacagcactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

348

Amino Acids

37.18

Weight (kDa)

6.79

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0031114)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001088.1_g000001 Rmu_sc0003318.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 793
AciI CCGC 6 cut(s) 396, 740, 758, 897, 936, 962
AclWI GGATC 3 cut(s) 594, 947, 986
AcsI RAATTY 1 cut(s) 130
AcyI GRCGYC 2 cut(s) 894, 1025
AfaI GTAC 3 cut(s) 213, 598, 830
AfiI CCNNNNNNNGG 1 cut(s) 553
AgsI TTSAA 5 cut(s) 37, 136, 356, 413, 903
AhdI GACNNNNNGTC 1 cut(s) 1024
AjnI CCWGG 3 cut(s) 58, 358, 713
AluBI AGCT 8 cut(s) 14, 65, 125, 195, 284, 327, 366, 846
AluI AGCT 8 cut(s) 14, 65, 125, 195, 284, 327, 366, 846
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 2 cut(s) 215, 478
AlwI GGATC 3 cut(s) 594, 947, 986
AlwNI CAGNNNCTG 2 cut(s) 59, 125
AoxI GGCC 2 cut(s) 188, 716
ApeKI GCWGC 5 cut(s) 14, 119, 122, 923, 964
ApoI RAATTY 1 cut(s) 130
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
AspS9I GGNCC 2 cut(s) 56, 717
AsuC2I CCSGG 1 cut(s) 1022
AsuHPI GGTGA 1 cut(s) 878
AvaII GGWCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 835, 867
BbsI GAAGAC 2 cut(s) 25, 1020
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 4 cut(s) 131, 134, 910, 951
BccI CCATC 3 cut(s) 263, 424, 787
BceAI ACGGC 2 cut(s) 750, 923
BciT130I CCWGG 3 cut(s) 60, 360, 715
BciVI GTATCC 2 cut(s) 235, 492
BcnI CCSGG 1 cut(s) 1022
BcoDI GTCTC 2 cut(s) 215, 478
BfaI CTAG 3 cut(s) 215, 254, 612
BfuI GTATCC 2 cut(s) 235, 492
BisI GCNGC 7 cut(s) 15, 120, 123, 740, 897, 924, 965
BlsI GCNGC 7 cut(s) 16, 121, 124, 741, 898, 925, 966
Bme1390I CCNGG 4 cut(s) 60, 360, 715, 1022
Bme18I GGWCC 1 cut(s) 56
BmeRI GACNNNNNGTC 1 cut(s) 1024
BmgT120I GGNCC 2 cut(s) 56, 717
BmrFI CCNGG 4 cut(s) 60, 360, 715, 1022
BpiI GAAGAC 2 cut(s) 25, 1020
BpmI CTGGAG 1 cut(s) 81
Bpu10I CCTNAGC 2 cut(s) 280, 693
BpuEI CTTGAG 2 cut(s) 181, 513
BpuMI CCSGG 1 cut(s) 1022
BsaBI GATNNNNATC 1 cut(s) 996
BsaHI GRCGYC 2 cut(s) 894, 1025
BsaJI CCNNGG 2 cut(s) 359, 713
BsaWI WCCGGW 1 cut(s) 833
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse3DI GCAATG 2 cut(s) 454, 630
Bse8I GATNNNNATC 1 cut(s) 996
BseBI CCWGG 3 cut(s) 60, 360, 715
BseDI CCNNGG 2 cut(s) 359, 713
BseGI GGATG 1 cut(s) 439
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 1 cut(s) 553
BseMI GCAATG 2 cut(s) 454, 630
BseMII CTCAG 2 cut(s) 117, 684
BseRI GAGGAG 2 cut(s) 456, 948
BseXI GCAGC 4 cut(s) 131, 134, 910, 951
BsgI GTGCAG 1 cut(s) 986
BshFI GGCC 2 cut(s) 190, 718
BsiHKAI GWGCWC 1 cut(s) 67
BsiSI CCGG 2 cut(s) 834, 1022
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 2 cut(s) 215, 478
BsmFI GGGAC 1 cut(s) 624
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 2 cut(s) 190, 718
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 6 cut(s) 586, 655, 810, 952, 991, 997
BspACI CCGC 6 cut(s) 396, 740, 758, 897, 936, 962
BspANI GGCC 2 cut(s) 190, 718
BspCNI CTCAG 2 cut(s) 118, 685
BspPI GGATC 3 cut(s) 594, 947, 986
BsrDI GCAATG 2 cut(s) 454, 630
BsrGI TGTACA 1 cut(s) 828
BssECI CCNNGG 2 cut(s) 359, 713
BssMI GATC 6 cut(s) 586, 655, 810, 952, 991, 997
BssNI GRCGYC 2 cut(s) 894, 1025
Bst2UI CCWGG 3 cut(s) 60, 360, 715
Bst4CI ACNGT 4 cut(s) 211, 319, 349, 426
Bst6I CTCTTC 3 cut(s) 213, 408, 727
BstACI GRCGYC 2 cut(s) 894, 1025
BstAUI TGTACA 1 cut(s) 828
BstC8I GCNNGC 1 cut(s) 936
BstDEI CTNAG 4 cut(s) 126, 155, 280, 693
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 6 cut(s) 589, 658, 813, 955, 994, 1000
BstMAI GTCTC 2 cut(s) 215, 478
BstMBI GATC 6 cut(s) 586, 655, 810, 952, 991, 997
BstMWI GCNNNNNNNGC 3 cut(s) 11, 703, 940
BstNI CCWGG 3 cut(s) 60, 360, 715
BstSCI CCNGG 4 cut(s) 58, 358, 713, 1020
BstV1I GCAGC 4 cut(s) 131, 134, 910, 951
BstV2I GAAGAC 2 cut(s) 25, 1020
BstX2I RGATCY 1 cut(s) 991
BstYI RGATCY 1 cut(s) 991
BsuI GTATCC 2 cut(s) 235, 492
BsuRI GGCC 2 cut(s) 190, 718
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 4 cut(s) 315, 354, 573, 1042
Cac8I GCNNGC 1 cut(s) 936
CaiI CAGNNNCTG 2 cut(s) 59, 125
Cfr13I GGNCC 2 cut(s) 56, 717
CseI GACGC 2 cut(s) 902, 1014
Csp6I GTAC 3 cut(s) 212, 597, 829
CviAII CATG 4 cut(s) 68, 334, 386, 494
CviQI GTAC 3 cut(s) 212, 597, 829
DdeI CTNAG 4 cut(s) 126, 155, 280, 693
DpnI GATC 6 cut(s) 588, 657, 812, 954, 993, 999
DpnII GATC 6 cut(s) 586, 655, 810, 952, 991, 997
DriI GACNNNNNGTC 1 cut(s) 1024
Eam1104I CTCTTC 3 cut(s) 213, 408, 727
Eam1105I GACNNNNNGTC 1 cut(s) 1024
EarI CTCTTC 3 cut(s) 213, 408, 727
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 65
EcoO109I RGGNCCY 2 cut(s) 56, 717
EcoRII CCWGG 3 cut(s) 58, 358, 713
EcoT38I GRGCYC 1 cut(s) 67
FaeI CATG 4 cut(s) 71, 337, 389, 497
FaqI GGGAC 1 cut(s) 624
FatI CATG 4 cut(s) 67, 333, 385, 493
FauI CCCGC 1 cut(s) 943
FblI GTMKAC 1 cut(s) 793
Fnu4HI GCNGC 7 cut(s) 15, 120, 123, 740, 897, 924, 965
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 7 cut(s) 15, 120, 123, 740, 897, 924, 965
FspBI CTAG 3 cut(s) 215, 254, 612
GluI GCNGC 7 cut(s) 15, 120, 123, 740, 897, 924, 965
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 2 cut(s) 190, 718
HapII CCGG 2 cut(s) 834, 1022
HgaI GACGC 2 cut(s) 902, 1014
Hin1I GRCGYC 2 cut(s) 894, 1025
Hin1II CATG 4 cut(s) 71, 337, 389, 497
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 5 cut(s) 109, 150, 602, 637, 751
HpaI GTTAAC 1 cut(s) 300
HpaII CCGG 2 cut(s) 834, 1022
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 3 cut(s) 300, 794, 831
Hpy188I TCNGA 1 cut(s) 874
Hpy188III TCNNGA 2 cut(s) 54, 995
Hpy8I GTNNAC 3 cut(s) 300, 794, 831
Hpy99I CGWCG 4 cut(s) 739, 748, 751, 896
HpyAV CCTTC 2 cut(s) 398, 831
HpyCH4III ACNGT 4 cut(s) 211, 319, 349, 426
HpyCH4IV ACGT 1 cut(s) 972
HpyCH4V TGCA 5 cut(s) 17, 119, 163, 967, 1003
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 703, 940
HpyF3I CTNAG 4 cut(s) 126, 155, 280, 693
HpySE526I ACGT 1 cut(s) 972
Hsp92I GRCGYC 2 cut(s) 894, 1025
Hsp92II CATG 4 cut(s) 71, 337, 389, 497
KspAI GTTAAC 1 cut(s) 300
Kzo9I GATC 6 cut(s) 586, 655, 810, 952, 991, 997
LmnI GCTCC 4 cut(s) 62, 289, 363, 703
Lsp1109I GCAGC 4 cut(s) 131, 134, 910, 951
MaeI CTAG 3 cut(s) 215, 254, 612
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 2 cut(s) 70, 799
MalI GATC 6 cut(s) 588, 657, 812, 954, 993, 999
MboI GATC 6 cut(s) 586, 655, 810, 952, 991, 997
MboII GAAGA 6 cut(s) 25, 230, 278, 425, 714, 1020
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 991
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 5 cut(s) 49, 84, 130, 459, 534
MlyI GAGTC 3 cut(s) 159, 646, 745
MmeI TCCRAC 3 cut(s) 418, 795, 897
MnlI CCTC 9 cut(s) 409, 434, 437, 547, 730, 875, 880, 966, 969
MseI TTAA 8 cut(s) 146, 233, 299, 339, 375, 501, 662, 975
MslI CAYNNNNRTG 1 cut(s) 492
MspA1I CMGCKG 2 cut(s) 125, 964
MspI CCGG 2 cut(s) 834, 1022
MspR9I CCNGG 4 cut(s) 60, 360, 715, 1022
MunI CAATTG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 163
MvaI CCWGG 3 cut(s) 60, 360, 715
MwoI GCNNNNNNNGC 3 cut(s) 11, 703, 940
NciI CCSGG 1 cut(s) 1022
NdeII GATC 6 cut(s) 586, 655, 810, 952, 991, 997
NlaIII CATG 4 cut(s) 71, 337, 389, 497
NmuCI GTSAC 2 cut(s) 70, 799
PcsI WCGNNNNNNNCGW 2 cut(s) 740, 743
PctI GAATGC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 109, 602
PkrI GCNGC 7 cut(s) 16, 121, 124, 741, 898, 925, 966
PleI GAGTC 3 cut(s) 158, 645, 745
PpsI GAGTC 3 cut(s) 158, 645, 745
PpuMI RGGWCCY 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 67
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 3 cut(s) 58, 358, 713
PspGI CCWGG 3 cut(s) 58, 358, 713
PspPI GGNCC 2 cut(s) 56, 717
PspPPI RGGWCCY 1 cut(s) 56
PstNI CAGNNNCTG 2 cut(s) 59, 125
PsuI RGATCY 1 cut(s) 991
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 3 cut(s) 213, 598, 830
RsaNI GTAC 3 cut(s) 212, 597, 829
RseI CAYNNNNRTG 1 cut(s) 492
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 8 cut(s) 146, 233, 299, 339, 375, 501, 662, 975
SatI GCNGC 7 cut(s) 15, 120, 123, 740, 897, 924, 965
Sau3AI GATC 6 cut(s) 586, 655, 810, 952, 991, 997
Sau96I GGNCC 2 cut(s) 56, 717
SchI GAGTC 3 cut(s) 159, 646, 745
ScrFI CCNGG 4 cut(s) 60, 360, 715, 1022
SduI GDGCHC 1 cut(s) 67
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 2 cut(s) 196, 528
SmoI CTYRAG 2 cut(s) 196, 528
Sse9I AATT 5 cut(s) 49, 84, 130, 459, 534
SsiI CCGC 6 cut(s) 396, 740, 758, 897, 936, 962
SspI AATATT 1 cut(s) 144
SspMI CTAG 3 cut(s) 215, 254, 612
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 4 cut(s) 58, 358, 713, 1020
TaaI ACNGT 4 cut(s) 211, 319, 349, 426
TaiI ACGT 1 cut(s) 975
TaqI TCGA 4 cut(s) 21, 507, 951, 1017
TasI AATT 5 cut(s) 49, 84, 130, 459, 534
TatI WGTACW 2 cut(s) 211, 828
TauI GCSGC 2 cut(s) 742, 899
TfiI GAWTC 2 cut(s) 109, 602
Tru1I TTAA 8 cut(s) 146, 233, 299, 339, 375, 501, 662, 975
Tru9I TTAA 8 cut(s) 146, 233, 299, 339, 375, 501, 662, 975
TscAI CASTG 3 cut(s) 322, 354, 573
TseFI GTSAC 2 cut(s) 70, 799
TseI GCWGC 5 cut(s) 14, 119, 122, 923, 964
Tsp45I GTSAC 2 cut(s) 70, 799
TspDTI ATGAA 2 cut(s) 279, 482
TspRI CASTG 3 cut(s) 322, 354, 573
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 793
XspI CTAG 3 cut(s) 215, 254, 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.