Rmu_sc0003562.1_g000003

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003562.1
OG0031270
Physical Location & Seq
Forward (+)
7112 .. 7696
585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
atggacaagattcaggaagttgttcctaaacttgacaaccagccagagcttctgcaaaagctttcagaagggaccttcacgctgaggattatgtatgagcagtttcagaacttgcttcaaatgggcccacttagccagctgacttcaatgcttccaggtgctgagctaatgccaaaaggtaatgataaggaaagccaggccaaaatcaagcggtacatgaccatgatggactcgatgacaaatgaagagttggacagcacaaatacaaaaatcattactgagtctcgtatggtgcgaatagcacgaggttctggtcgtctggtaagagaagtgatggagatgtttgaagagtacaagcgccttgctaaaatatggagcaaaatgaaaggaattaagattcctaagaagggtggtatcagtccgctaaaccaaaactttaatgcacagcaaatgagcaaagtcctacctccccagatgttgaagcagattggtggcatgggtggcttacagaacttgatgaagcaaatgggttcaatgggttcagctaaagaaatgatgggaatgcttggaggcggggacaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SRP54 SRP54_N SRP_SPB

Protein Analysis

194

Amino Acids

21.86

Weight (kDa)

9.64

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRP_SPB PF02978 25 - 124 1.1e-27 Signal peptide binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0031270)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003562.1_g000003 Rmu_sc0003562.1_g000005

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 211, 422, 573
AfaI GTAC 2 cut(s) 215, 353
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 5 cut(s) 119, 147, 347, 481, 534
AjnI CCWGG 2 cut(s) 154, 195
AluBI AGCT 5 cut(s) 49, 61, 139, 166, 545
AluI AGCT 5 cut(s) 49, 61, 139, 166, 545
Alw26I GTCTC 1 cut(s) 288
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 2 cut(s) 124, 198
ApaI GGGCCC 1 cut(s) 128
Asp700I GAANNNNTTC 1 cut(s) 21
AspLEI GCGC 1 cut(s) 360
AspS9I GGNCC 3 cut(s) 72, 124, 125
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 128
BauI CACGAG 1 cut(s) 303
BbvCI CCTCAGC 1 cut(s) 83
BccI CCATC 3 cut(s) 220, 328, 550
BciT130I CCWGG 2 cut(s) 156, 197
BcoDI GTCTC 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 361
BlpI GCTNAGC 1 cut(s) 162
Bme1390I CCNGG 2 cut(s) 156, 197
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 72, 124, 125
BmiI GGNNCC 2 cut(s) 73, 126
BmrFI CCNGG 2 cut(s) 156, 197
Bpu10I CCTNAGC 1 cut(s) 83
Bpu1102I GCTNAGC 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 407
BseBI CCWGG 2 cut(s) 156, 197
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMII CTCAG 3 cut(s) 74, 153, 270
BseSI GKGCMC 1 cut(s) 128
BshFI GGCC 2 cut(s) 126, 200
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 567
BsnI GGCC 2 cut(s) 126, 200
Bsp120I GGGCCC 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 128
Bsp1720I GCTNAGC 1 cut(s) 162
BspACI CCGC 3 cut(s) 211, 422, 573
BspANI GGCC 2 cut(s) 126, 200
BspCNI CTCAG 3 cut(s) 75, 154, 271
BspLI GGNNCC 2 cut(s) 73, 126
BssSI CACGAG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 303
Bst2UI CCWGG 2 cut(s) 156, 197
Bst6I CTCTTC 2 cut(s) 240, 342
BstC8I GCNNGC 1 cut(s) 137
BstDEI CTNAG 5 cut(s) 83, 131, 162, 279, 402
BstH2I RGCGCY 1 cut(s) 361
BstHHI GCGC 1 cut(s) 360
BstMAI GTCTC 1 cut(s) 288
BstMWI GCNNNNNNNGC 2 cut(s) 132, 501
BstNI CCWGG 2 cut(s) 156, 197
BstSCI CCNGG 2 cut(s) 154, 195
BstSLI GKGCMC 1 cut(s) 128
BsuRI GGCC 2 cut(s) 126, 200
Cac8I GCNNGC 1 cut(s) 137
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 1 cut(s) 360
Cfr13I GGNCC 3 cut(s) 72, 124, 125
Csp6I GTAC 2 cut(s) 214, 352
CviAII CATG 3 cut(s) 217, 223, 496
CviQI GTAC 2 cut(s) 214, 352
DdeI CTNAG 5 cut(s) 83, 131, 162, 279, 402
Eam1104I CTCTTC 2 cut(s) 240, 342
EarI CTCTTC 2 cut(s) 240, 342
Eco24I GRGCYC 1 cut(s) 128
Eco47I GGWCC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 72
EcoRII CCWGG 2 cut(s) 154, 195
EcoT38I GRGCYC 1 cut(s) 128
FaeI CATG 3 cut(s) 220, 226, 499
FaiI YATR 7 cut(s) 92, 96, 218, 224, 290, 373, 497
FaqI GGGAC 1 cut(s) 85
FatI CATG 3 cut(s) 216, 222, 495
FauI CCCGC 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 128
GlaI GCGC 1 cut(s) 359
HaeII RGCGCY 1 cut(s) 361
HaeIII GGCC 2 cut(s) 126, 200
HhaI GCGC 1 cut(s) 360
Hin1II CATG 3 cut(s) 220, 226, 499
Hin6I GCGC 1 cut(s) 358
HinP1I GCGC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 4 cut(s) 10, 230, 281, 397
Hpy188I TCNGA 2 cut(s) 67, 108
Hpy188III TCNNGA 1 cut(s) 14
HpyAV CCTTC 3 cut(s) 62, 85, 400
HpyCH4V TGCA 2 cut(s) 55, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 501
HpyF3I CTNAG 5 cut(s) 83, 131, 162, 279, 402
Hsp92II CATG 3 cut(s) 220, 226, 499
HspAI GCGC 1 cut(s) 358
LmnI GCTCC 1 cut(s) 375
MboII GAAGA 2 cut(s) 257, 359
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 1 cut(s) 390
MlyI GAGTC 2 cut(s) 224, 290
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 4 cut(s) 78, 299, 477, 563
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 2 cut(s) 393, 438
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 139
MspR9I CCNGG 2 cut(s) 156, 197
Mva1269I GAATGC 1 cut(s) 567
MvaI CCWGG 2 cut(s) 156, 197
MwoI GCNNNNNNNGC 2 cut(s) 132, 501
NlaIII CATG 3 cut(s) 220, 226, 499
NlaIV GGNNCC 2 cut(s) 73, 126
PcsI WCGNNNNNNNCGW 1 cut(s) 292
PctI GAATGC 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 2 cut(s) 10, 397
PleI GAGTC 2 cut(s) 224, 289
PpsI GAGTC 2 cut(s) 224, 289
PpuMI RGGWCCY 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 154, 195
PspGI CCWGG 2 cut(s) 154, 195
PspN4I GGNNCC 2 cut(s) 73, 126
PspOMI GGGCCC 1 cut(s) 124
PspPI GGNCC 3 cut(s) 72, 124, 125
PspPPI RGGWCCY 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 161
PvuII CAGCTG 1 cut(s) 139
RsaI GTAC 2 cut(s) 215, 353
RsaNI GTAC 2 cut(s) 214, 352
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 2 cut(s) 393, 438
Sau96I GGNCC 3 cut(s) 72, 124, 125
SchI GAGTC 2 cut(s) 224, 290
ScrFI CCNGG 2 cut(s) 156, 197
SduI GDGCHC 1 cut(s) 128
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 221
Sse9I AATT 1 cut(s) 390
SsiI CCGC 3 cut(s) 211, 422, 573
StyD4I CCNGG 2 cut(s) 154, 195
TaqI TCGA 1 cut(s) 233
TasI AATT 1 cut(s) 390
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 2 cut(s) 10, 397
Tru1I TTAA 2 cut(s) 393, 438
Tru9I TTAA 2 cut(s) 393, 438
TspDTI ATGAA 3 cut(s) 258, 398, 533
VpaK11BI GGWCC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.