Rmu_sc0003630.1_g000006
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003630.1
OG0025400
Physical Location & Seq
Forward (+)
26687 .. 29802
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2151 bp
atggtggcagtaacgctagcatctgctcaggagcccaggcctgcaacctgctcggaccgctgtggtgatgtcgaaattccatatccattcggcatagagcctcgctgttatctggagttgccgggacaagaagaacagcaattcaaggtcacttgtgaccgggccaccacgccaccattactcaagttcttgaacggtgatatccttaatcaaagtattaccaacatttctgtaggtgtgaatgagatgcaagtaatgttgccaacatcccgaaactgctataccaacaattcatacgaagaatctttgtacagacacagtcagctcatccttccctctggttataccatctctgataaaaacacagtttacatcattgggtgtaacaaaataacactatatcaaggttttgttagccaagtcgacccagagccaaaagaaatcgagaaactggggacaagcggcgtgagcttgtgtcaagatcttcttggagagctcccccagaattgcagtggcatcgggtgtaccaaaggttccattgtaagtggactgcacaatattacgttggatgtgtggacaatcggtacaggccttggaagcacatcacagtggggactagactacccgtgtagctacggtttcattatggatgaacgcaatttcaccttcgccgggaatgcaacttttagagagctagacagcaccacaaggccggagcttccggtggttgttaactgggaaattgggaaggacaacagtgaagcagcctctaataatcaaatttcttatgcatgcaaggccgcaaatactgaatgtgtcaacggctcttctggtggatatttttgccggtgccagaaaggttaccaggggaacccatacgtccttgatggttgcgaaggtgtctgtttaagctttgtggtggtacttgtcctaacattttggatatattgcggaatgaagagaagaaaattcaggcaactcaaggagaagtacttcaaagagaatggtggcttattgttacaacaaaagttggagaatttcaatggacctcaagcagccaaaatctttacgaaagaagagcttaagaatgccacaaacaacttcgatgacagcggaaaaattggtgaaggaggctatggatttgtttacaaaggaacgttgccagataaaaaggaggttgctattaaggtgtcaaagagcaatgctccaatgacccaaagtaatcagttcattaatgagatagcaacagaaacagcaggatcactagcatacttgcactactccacttccatgcaaatcgtgcaccgagatgtgaaagcaaccaatatactactggatgagaacttcatagcaaaagtgtcggactttggagcttcaaaattggttcctgaagatcaaaatcagttgtctactttagtgcaagggacgctagtgttaagagtatggggtcaaagtcaacataaatgtttttcccttttctctctctcgttcttcttcttcttcattctcaatagcctccattaccatgaatttcaacatggtatcaaagcccatggttgtctgaccttcgatccttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcaagttctccgctgcaacttcgattcttcatcatcaaaaccttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-H2C2

Protein Analysis

716

Amino Acids

79.63

Weight (kDa)

8.18

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 17 - 85 1.5e-08 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 408 - 470 5.7e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 413 - 468 4.4e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025400)

Species Orthologous Gene IDs
rosa_laevigata RLG00000029327 RLG00000032270
rosa_multiflora Rmu_sc0003630.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 56
AccB1I GGYRCC 1 cut(s) 849
AccI GTMKAC 2 cut(s) 423, 1409
AclI AACGTT 1 cut(s) 1157
AclWI GGATC 2 cut(s) 1267, 1565
AcsI RAATTY 5 cut(s) 75, 780, 968, 1036, 1529
AcuI CTGAAG 1 cut(s) 1410
AfaI GTAC 5 cut(s) 311, 526, 586, 924, 992
AfiI CCNNNNNNNGG 1 cut(s) 672
AflII CTTAAG 1 cut(s) 1082
AgsI TTSAA 6 cut(s) 145, 193, 997, 1042, 1377, 1535
AjnI CCWGG 2 cut(s) 35, 864
AluBI AGCT 9 cut(s) 325, 471, 496, 633, 694, 718, 912, 1081, 1373
AluI AGCT 9 cut(s) 325, 471, 496, 633, 694, 718, 912, 1081, 1373
Alw21I GWGCWC 2 cut(s) 498, 1305
Alw44I GTGCAC 1 cut(s) 1301
AlwI GGATC 2 cut(s) 1267, 1565
AoxI GGCC 5 cut(s) 38, 162, 589, 710, 798
ApaLI GTGCAC 1 cut(s) 1301
ApoI RAATTY 5 cut(s) 75, 780, 968, 1036, 1529
AseI ATTAAT 1 cut(s) 1233
Asp700I GAANNNNTTC 1 cut(s) 992
AspS9I GGNCC 3 cut(s) 55, 162, 1046
AsuC2I CCSGG 3 cut(s) 123, 161, 673
AsuHPI GGTGA 4 cut(s) 77, 209, 655, 1136
AsuNHI GCTAGC 1 cut(s) 16
AvaII GGWCC 2 cut(s) 55, 1046
BaeGI GKGCMC 1 cut(s) 1305
BanI GGYRCC 1 cut(s) 849
BanII GRGCYC 2 cut(s) 36, 498
Bbv12I GWGCWC 2 cut(s) 498, 1305
BccI CCATC 2 cut(s) 356, 881
BceAI ACGGC 1 cut(s) 838
BcgI CGANNNNNNTGC 6 cut(s) 33, 67, 499, 533, 1341, 1375
BciT130I CCWGG 2 cut(s) 37, 866
BcnI CCSGG 3 cut(s) 123, 161, 673
BfaI CTAG 5 cut(s) 17, 617, 695, 1265, 1430
BfmI CTRYAG 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 1082
BfuAI ACCTGC 1 cut(s) 56
BglII AGATCT 1 cut(s) 481
BmcAI AGTACT 1 cut(s) 992
Bme1390I CCNGG 5 cut(s) 37, 123, 161, 673, 866
Bme18I GGWCC 2 cut(s) 55, 1046
BmgT120I GGNCC 3 cut(s) 55, 162, 1046
BmiI GGNNCC 5 cut(s) 33, 535, 851, 872, 1386
BmrFI CCNGG 5 cut(s) 37, 123, 161, 673, 866
BmrI ACTGGG 2 cut(s) 461, 745
BmsI GCATC 3 cut(s) 29, 237, 525
BmtI GCTAGC 1 cut(s) 20
BmuI ACTGGG 2 cut(s) 461, 745
BplI GAGNNNNNCTC 2 cut(s) 1189, 1221
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 27
BpuEI CTTGAG 3 cut(s) 167, 965, 1035
BpuMI CCSGG 3 cut(s) 123, 161, 673
BsaBI GATNNNNATC 1 cut(s) 1398
BsaJI CCNNGG 4 cut(s) 35, 592, 865, 1552
BsaWI WCCGGW 1 cut(s) 721
Bsc4I CCNNNNNNNGG 1 cut(s) 672
Bse118I RCCGGY 1 cut(s) 846
Bse1I ACTGG 3 cut(s) 456, 740, 1338
Bse3DI GCAATG 1 cut(s) 1207
Bse8I GATNNNNATC 1 cut(s) 1398
BseBI CCWGG 2 cut(s) 37, 866
BseDI CCNNGG 4 cut(s) 35, 592, 865, 1552
BseGI GGATG 5 cut(s) 266, 327, 574, 655, 1342
BseJI GATNNNNATC 1 cut(s) 1398
BseLI CCNNNNNNNGG 1 cut(s) 672
BseMI GCAATG 1 cut(s) 1207
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 3 cut(s) 456, 740, 1338
BseSI GKGCMC 1 cut(s) 1305
BsgI GTGCAG 1 cut(s) 536
BshFI GGCC 5 cut(s) 40, 164, 591, 712, 800
BshNI GGYRCC 1 cut(s) 849
BsiHKAI GWGCWC 2 cut(s) 498, 1305
BsiSI CCGG 6 cut(s) 122, 160, 672, 713, 722, 847
BslFI GGGAC 4 cut(s) 138, 469, 627, 1438
BslI CCNNNNNNNGG 1 cut(s) 672
BsmFI GGGAC 4 cut(s) 138, 469, 627, 1438
BsmI GAATGC 2 cut(s) 682, 1093
BsnI GGCC 5 cut(s) 40, 164, 591, 712, 800
Bsp1286I GDGCHC 3 cut(s) 36, 498, 1305
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 4 cut(s) 481, 1259, 1393, 1570
Bsp19I CCATGG 1 cut(s) 1552
BspANI GGCC 5 cut(s) 40, 164, 591, 712, 800
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 5 cut(s) 33, 535, 851, 872, 1386
BspMI ACCTGC 1 cut(s) 56
BspOI GCTAGC 1 cut(s) 20
BspPI GGATC 2 cut(s) 1267, 1565
BspQI GCTCTTC 2 cut(s) 832, 1071
BspT107I GGYRCC 1 cut(s) 849
BspTI CTTAAG 1 cut(s) 1082
BsrDI GCAATG 1 cut(s) 1207
BsrFI RCCGGY 1 cut(s) 846
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 3 cut(s) 456, 740, 1338
BssAI RCCGGY 1 cut(s) 846
BssECI CCNNGG 4 cut(s) 35, 592, 865, 1552
BssMI GATC 4 cut(s) 481, 1259, 1393, 1570
BssT1I CCWWGG 2 cut(s) 592, 1552
Bst2UI CCWGG 2 cut(s) 37, 866
Bst4CI ACNGT 6 cut(s) 197, 320, 367, 609, 638, 758
Bst6I CTCTTC 3 cut(s) 832, 953, 1071
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 1 cut(s) 1300
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 3 cut(s) 18, 42, 793
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 1 cut(s) 1552
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 5 cut(s) 266, 327, 574, 655, 1342
BstKTI GATC 4 cut(s) 484, 1262, 1396, 1573
BstMBI GATC 4 cut(s) 481, 1259, 1393, 1570
BstMWI GCNNNNNNNGC 7 cut(s) 57, 468, 597, 677, 797, 1300, 1426
BstNI CCWGG 2 cut(s) 37, 866
BstNSI RCATGY 1 cut(s) 795
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 5 cut(s) 35, 121, 159, 671, 864
BstSFI CTRYAG 1 cut(s) 231
BstSLI GKGCMC 1 cut(s) 1305
BstX2I RGATCY 1 cut(s) 481
BstYI RGATCY 1 cut(s) 481
BsuRI GGCC 5 cut(s) 40, 164, 591, 712, 800
BtgI CCRYGG 1 cut(s) 1552
BtsCI GGATG 5 cut(s) 266, 327, 574, 655, 1342
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 3 cut(s) 517, 614, 763
BveI ACCTGC 1 cut(s) 56
Cac8I GCNNGC 3 cut(s) 18, 42, 793
Cfr10I RCCGGY 1 cut(s) 846
Cfr13I GGNCC 3 cut(s) 55, 162, 1046
CpoI CGGWCCG 1 cut(s) 55
CseI GACGC 1 cut(s) 1435
Csp6I GTAC 5 cut(s) 310, 525, 585, 923, 991
CspI CGGWCCG 1 cut(s) 55
CviAII CATG 5 cut(s) 792, 1291, 1526, 1538, 1553
CviQI GTAC 5 cut(s) 310, 525, 585, 923, 991
DdeI CTNAG 1 cut(s) 27
DpnI GATC 4 cut(s) 483, 1261, 1395, 1572
DpnII GATC 4 cut(s) 481, 1259, 1393, 1570
Eam1104I CTCTTC 3 cut(s) 832, 953, 1071
EarI CTCTTC 3 cut(s) 832, 953, 1071
Ecl136II GAGCTC 1 cut(s) 496
Eco130I CCWWGG 2 cut(s) 592, 1552
Eco147I AGGCCT 2 cut(s) 40, 591
Eco24I GRGCYC 2 cut(s) 36, 498
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 55, 1046
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 1410
Eco91I GGTNACC 1 cut(s) 860
EcoICRI GAGCTC 1 cut(s) 496
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 2 cut(s) 35, 864
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 2 cut(s) 592, 1552
EcoT22I ATGCAT 1 cut(s) 793
EcoT38I GRGCYC 2 cut(s) 36, 498
ErhI CCWWGG 2 cut(s) 592, 1552
FaeI CATG 5 cut(s) 795, 1294, 1529, 1541, 1556
FalI AAGNNNNNCTT 4 cut(s) 471, 503, 1065, 1097
FaqI GGGAC 4 cut(s) 138, 469, 627, 1438
FatI CATG 5 cut(s) 791, 1290, 1525, 1537, 1552
FblI GTMKAC 2 cut(s) 423, 1409
FokI GGATG 5 cut(s) 253, 314, 581, 662, 1349
FriOI GRGCYC 2 cut(s) 36, 498
FspBI CTAG 5 cut(s) 17, 617, 695, 1265, 1430
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 5 cut(s) 40, 164, 591, 712, 800
HapII CCGG 6 cut(s) 122, 160, 672, 713, 722, 847
HgaI GACGC 1 cut(s) 1435
Hin1II CATG 5 cut(s) 795, 1294, 1529, 1541, 1556
HincII GTYRAC 4 cut(s) 424, 733, 820, 1457
HindII GTYRAC 4 cut(s) 424, 733, 820, 1457
HindIII AAGCTT 1 cut(s) 910
HpaI GTTAAC 1 cut(s) 733
HpaII CCGG 6 cut(s) 122, 160, 672, 713, 722, 847
HphI GGTGA 4 cut(s) 77, 209, 655, 1136
Hpy188I TCNGA 4 cut(s) 55, 355, 1363, 1563
Hpy188III TCNNGA 7 cut(s) 29, 113, 190, 270, 445, 479, 1388
HpyAV CCTTC 7 cut(s) 341, 676, 742, 890, 1121, 1576, 1584
HpyCH4III ACNGT 6 cut(s) 197, 320, 367, 609, 638, 758
HpyCH4IV ACGT 3 cut(s) 563, 879, 1157
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 468, 597, 677, 797, 1300, 1426
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 3 cut(s) 563, 879, 1157
Hsp92II CATG 5 cut(s) 795, 1294, 1529, 1541, 1556
KspAI GTTAAC 1 cut(s) 733
Kzo9I GATC 4 cut(s) 481, 1259, 1393, 1570
LguI GCTCTTC 2 cut(s) 832, 1071
LmnI GCTCC 5 cut(s) 31, 501, 715, 1210, 1370
LweI GCATC 3 cut(s) 29, 237, 525
MaeI CTAG 5 cut(s) 17, 617, 695, 1265, 1430
MaeII ACGT 3 cut(s) 563, 879, 1157
MaeIII GTNAC 6 cut(s) 10, 148, 155, 383, 860, 1017
MalI GATC 4 cut(s) 483, 1261, 1395, 1572
MboI GATC 4 cut(s) 481, 1259, 1393, 1570
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 3 cut(s) 36, 498, 1305
MmeI TCCRAC 3 cut(s) 546, 1011, 1341
MnlI CCTC 7 cut(s) 111, 346, 778, 1059, 1124, 1168, 1526
Mph1103I ATGCAT 1 cut(s) 793
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 8 cut(s) 207, 732, 908, 1083, 1185, 1233, 1436, 2149
MslI CAYNNNNRTG 4 cut(s) 607, 1289, 1308, 1524
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 6 cut(s) 122, 160, 672, 713, 722, 847
MspR9I CCNGG 5 cut(s) 37, 123, 161, 673, 866
Mva1269I GAATGC 2 cut(s) 682, 1093
MvaI CCWGG 2 cut(s) 37, 866
MwoI GCNNNNNNNGC 7 cut(s) 57, 468, 597, 677, 797, 1300, 1426
NciI CCSGG 3 cut(s) 123, 161, 673
NcoI CCATGG 1 cut(s) 1552
NdeII GATC 4 cut(s) 481, 1259, 1393, 1570
NheI GCTAGC 1 cut(s) 16
NlaIII CATG 5 cut(s) 795, 1294, 1529, 1541, 1556
NlaIV GGNNCC 5 cut(s) 33, 535, 851, 872, 1386
NmuCI GTSAC 2 cut(s) 148, 155
NsiI ATGCAT 1 cut(s) 793
NspI RCATGY 1 cut(s) 795
PaeI GCATGC 1 cut(s) 795
PceI AGGCCT 2 cut(s) 40, 591
PciSI GCTCTTC 2 cut(s) 832, 1071
PctI GAATGC 2 cut(s) 682, 1093
PdmI GAANNNNTTC 1 cut(s) 992
PflFI GACNNNGTC 1 cut(s) 318
PshBI ATTAAT 1 cut(s) 1233
Psp124BI GAGCTC 1 cut(s) 498
Psp1406I AACGTT 1 cut(s) 1157
Psp6I CCWGG 2 cut(s) 35, 864
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 2 cut(s) 35, 864
PspN4I GGNNCC 5 cut(s) 33, 535, 851, 872, 1386
PspPI GGNCC 3 cut(s) 55, 162, 1046
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
PsuI RGATCY 1 cut(s) 481
PsyI GACNNNGTC 1 cut(s) 318
RsaI GTAC 5 cut(s) 311, 526, 586, 924, 992
RsaNI GTAC 5 cut(s) 310, 525, 585, 923, 991
RseI CAYNNNNRTG 4 cut(s) 607, 1289, 1308, 1524
Rsr2I CGGWCCG 1 cut(s) 55
RsrII CGGWCCG 1 cut(s) 55
SacI GAGCTC 1 cut(s) 498
SalI GTCGAC 1 cut(s) 422
SapI GCTCTTC 2 cut(s) 832, 1071
SaqAI TTAA 8 cut(s) 207, 732, 908, 1083, 1185, 1233, 1436, 2149
Sau3AI GATC 4 cut(s) 481, 1259, 1393, 1570
Sau96I GGNCC 3 cut(s) 55, 162, 1046
ScaI AGTACT 1 cut(s) 992
ScrFI CCNGG 5 cut(s) 37, 123, 161, 673, 866
SduI GDGCHC 3 cut(s) 36, 498, 1305
SfaNI GCATC 3 cut(s) 29, 237, 525
SfcI CTRYAG 1 cut(s) 231
SinI GGWCC 2 cut(s) 55, 1046
SmiMI CAYNNNNRTG 4 cut(s) 607, 1289, 1308, 1524
SmlI CTYRAG 4 cut(s) 182, 980, 1050, 1082
SmoI CTYRAG 4 cut(s) 182, 980, 1050, 1082
SphI GCATGC 1 cut(s) 795
SseBI AGGCCT 2 cut(s) 40, 591
SspI AATATT 1 cut(s) 559
SspMI CTAG 5 cut(s) 17, 617, 695, 1265, 1430
SstI GAGCTC 1 cut(s) 498
StuI AGGCCT 2 cut(s) 40, 591
StyD4I CCNGG 5 cut(s) 35, 121, 159, 671, 864
StyI CCWWGG 2 cut(s) 592, 1552
TaaI ACNGT 6 cut(s) 197, 320, 367, 609, 638, 758
TaiI ACGT 3 cut(s) 566, 882, 1160
TatI WGTACW 2 cut(s) 309, 990
TauI GCSGC 2 cut(s) 465, 803
Tru1I TTAA 8 cut(s) 207, 732, 908, 1083, 1185, 1233, 1436, 2149
Tru9I TTAA 8 cut(s) 207, 732, 908, 1083, 1185, 1233, 1436, 2149
TscAI CASTG 3 cut(s) 517, 614, 763
TseFI GTSAC 2 cut(s) 148, 155
Tsp45I GTSAC 2 cut(s) 148, 155
TspRI CASTG 3 cut(s) 517, 614, 763
Tth111I GACNNNGTC 1 cut(s) 318
Vha464I CTTAAG 1 cut(s) 1082
VneI GTGCAC 1 cut(s) 1301
VpaK11BI GGWCC 2 cut(s) 55, 1046
VspI ATTAAT 1 cut(s) 1233
XapI RAATTY 5 cut(s) 75, 780, 968, 1036, 1529
XceI RCATGY 1 cut(s) 795
XcmI CCANNNNNNNNNTGG 2 cut(s) 509, 1330
XmiI GTMKAC 2 cut(s) 423, 1409
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 5 cut(s) 17, 617, 695, 1265, 1430
ZrmI AGTACT 1 cut(s) 992
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.