Rmu_sc0004311.1_g000037

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004311.1
OG0031298
Physical Location & Seq
Reverse (-)
149307 .. 152232
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 702 bp
atggcaacctccaactcctctcgtactactgttgatttgaagctcatgattgatagaaagcgcaagaaagtcttgtttgctgaagcaggcaaggattttgaggattttctcactacaagaaaactgatcatttacgacgctgaatttacggcctcctccgccaaaatcaagtcactctctgtctccgagatcacaccccctcagctctccaacctctcaattgtcaaatccgcctccaccttctacttcgcctccgccttcgccagtgagccatcggaggcggagctccataatgatcaagagtttgtctgtgatggcaaagacgattccagtggtgaggaaaacacagagacccagcaaagcttcgtagagaggaagcatcagtttcctggaatgccaaatctcaagaaggatgtccttctaaaacctagggcaccagttggtggttctgataatgtttttcttcctttgttgaccaacaatgaatcaaatgctaagaagttttacgtgtcgactacgggagaaagggccagccatggaggcggctgtgtgagaggcgttgttacgtacatgataatggatgatttggaagtaaagcctatgtccaccatttcaagtataactctgcttaacaacttcaacgttaaggatgttggtacccttgaagagaaggtggttcctctagacatggataaggtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

233

Amino Acids

25.69

Weight (kDa)

5.36

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 172 - 227 1.9e-07 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031298)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004311.1_g000037
rosa_samantha Rh7DG039800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 2 cut(s) 433, 656
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 512
AciI CCGC 5 cut(s) 159, 231, 255, 281, 543
AclI AACGTT 1 cut(s) 642
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 3 cut(s) 25, 569, 658
AfiI CCNNNNNNNGG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 4 cut(s) 40, 615, 640, 665
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 4 cut(s) 43, 205, 286, 363
AluI AGCT 4 cut(s) 43, 205, 286, 363
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 2 cut(s) 187, 344
AoxI GGCC 2 cut(s) 150, 528
ApoI RAATTY 1 cut(s) 143
Asp718I GGTACC 1 cut(s) 656
AspA2I CCTAGG 1 cut(s) 428
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 528
AsuHPI GGTGA 1 cut(s) 347
AvrII CCTAGG 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 2 cut(s) 433, 656
BanII GRGCYC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 288
BbvCI CCTCAGC 1 cut(s) 201
BccI CCATC 2 cut(s) 280, 308
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 1 cut(s) 390
BclI TGATCA 2 cut(s) 126, 295
BcoDI GTCTC 2 cut(s) 187, 344
BfaI CTAG 2 cut(s) 429, 683
BglI GCCNNNNNGGC 1 cut(s) 540
BisI GCNGC 1 cut(s) 544
BlnI CCTAGG 1 cut(s) 428
BlsI GCNGC 1 cut(s) 545
Bme1390I CCNGG 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 528
BmiI GGNNCC 3 cut(s) 435, 658, 678
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 1 cut(s) 388
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 389
BsaAI YACGTR 2 cut(s) 508, 567
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 428, 535
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 3 cut(s) 264, 330, 437
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 428, 535
BseGI GGATG 3 cut(s) 418, 586, 655
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 3 cut(s) 264, 330, 437
BseRI GAGGAG 2 cut(s) 7, 145
BseSI GKGCMC 1 cut(s) 436
BseYI CCCAGC 1 cut(s) 354
BshFI GGCC 2 cut(s) 152, 530
BshNI GGYRCC 2 cut(s) 433, 656
BsiHKAI GWGCWC 1 cut(s) 288
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 2 cut(s) 187, 344
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 2 cut(s) 152, 530
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 288, 436
Bsp143I GATC 3 cut(s) 126, 189, 295
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 5 cut(s) 159, 231, 255, 281, 543
BspANI GGCC 2 cut(s) 152, 530
BspCNI CTCAG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 3 cut(s) 435, 658, 678
BspT107I GGYRCC 2 cut(s) 433, 656
BspTNI GGTCTC 1 cut(s) 344
BsrI ACTGG 3 cut(s) 264, 330, 437
BssECI CCNNGG 2 cut(s) 428, 535
BssMI GATC 3 cut(s) 126, 189, 295
BssT1I CCWWGG 2 cut(s) 428, 535
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 660
BstBAI YACGTR 2 cut(s) 508, 567
BstC8I GCNNGC 2 cut(s) 88, 532
BstDEI CTNAG 2 cut(s) 201, 495
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 3 cut(s) 418, 586, 655
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 3 cut(s) 129, 192, 298
BstMAI GTCTC 2 cut(s) 187, 344
BstMBI GATC 3 cut(s) 126, 189, 295
BstMWI GCNNNNNNNGC 2 cut(s) 158, 540
BstNI CCWGG 1 cut(s) 390
BstSCI CCNGG 1 cut(s) 388
BstSLI GKGCMC 1 cut(s) 436
BstSNI TACGTA 1 cut(s) 567
BsuRI GGCC 2 cut(s) 152, 530
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 3 cut(s) 418, 586, 655
BtsIMutI CAGTG 2 cut(s) 271, 337
Cac8I GCNNGC 2 cut(s) 88, 532
CciI TCATGA 1 cut(s) 45
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 1 cut(s) 528
CseI GACGC 1 cut(s) 146
Csp6I GTAC 3 cut(s) 24, 568, 657
CviAII CATG 4 cut(s) 46, 536, 571, 688
CviQI GTAC 3 cut(s) 24, 568, 657
DdeI CTNAG 2 cut(s) 201, 495
DpnI GATC 3 cut(s) 128, 191, 297
DpnII GATC 3 cut(s) 126, 189, 295
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
EciI GGCGGA 4 cut(s) 148, 220, 244, 296
Ecl136II GAGCTC 1 cut(s) 286
Eco105I TACGTA 1 cut(s) 567
Eco130I CCWWGG 2 cut(s) 428, 535
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 2 cut(s) 428, 535
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 2 cut(s) 428, 535
FaeI CATG 4 cut(s) 49, 539, 574, 691
FaiI YATR 7 cut(s) 47, 291, 537, 572, 602, 620, 689
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 4 cut(s) 45, 535, 570, 687
FbaI TGATCA 2 cut(s) 126, 295
FblI GTMKAC 1 cut(s) 512
Fnu4HI GCNGC 1 cut(s) 544
FokI GGATG 3 cut(s) 425, 593, 662
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 544
FspBI CTAG 2 cut(s) 429, 683
GlaI GCGC 1 cut(s) 62
GluI GCNGC 1 cut(s) 544
GsaI CCCAGC 1 cut(s) 358
HaeIII GGCC 2 cut(s) 152, 530
HgaI GACGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 63
Hin1II CATG 4 cut(s) 49, 539, 574, 691
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HincII GTYRAC 2 cut(s) 474, 513
HindII GTYRAC 2 cut(s) 474, 513
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 2 cut(s) 326, 485
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 3 cut(s) 474, 513, 606
Hpy188I TCNGA 3 cut(s) 187, 277, 451
Hpy188III TCNNGA 4 cut(s) 46, 299, 406, 683
Hpy8I GTNNAC 3 cut(s) 474, 513, 606
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 5 cut(s) 250, 268, 403, 428, 664
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 3 cut(s) 507, 566, 642
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 540
HpyF3I CTNAG 2 cut(s) 201, 495
HpySE526I ACGT 3 cut(s) 507, 566, 642
Hsp92II CATG 4 cut(s) 49, 539, 574, 691
HspAI GCGC 1 cut(s) 61
KpnI GGTACC 1 cut(s) 660
Ksp22I TGATCA 2 cut(s) 126, 295
Kzo9I GATC 3 cut(s) 126, 189, 295
LmnI GCTCC 2 cut(s) 283, 291
LpnPI CCDG 8 cut(s) 72, 277, 343, 368, 375, 402, 450, 544
LweI GCATC 1 cut(s) 388
MaeI CTAG 2 cut(s) 429, 683
MaeII ACGT 3 cut(s) 507, 566, 642
MaeIII GTNAC 2 cut(s) 171, 562
MalI GATC 3 cut(s) 128, 191, 297
MboI GATC 3 cut(s) 126, 189, 295
MboII GAAGA 2 cut(s) 455, 677
MfeI CAATTG 1 cut(s) 219
MhlI GDGCHC 2 cut(s) 288, 436
MluCI AATT 2 cut(s) 143, 219
MmeI TCCRAC 2 cut(s) 36, 234
MseI TTAA 2 cut(s) 630, 645
MslI CAYNNNNRTG 1 cut(s) 575
MspR9I CCNGG 1 cut(s) 390
MunI CAATTG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 158, 540
NcoI CCATGG 1 cut(s) 535
NdeII GATC 3 cut(s) 126, 189, 295
NlaIII CATG 4 cut(s) 49, 539, 574, 691
NlaIV GGNNCC 3 cut(s) 435, 658, 678
NmuCI GTSAC 1 cut(s) 171
PagI TCATGA 1 cut(s) 45
PctI GAATGC 1 cut(s) 399
PfeI GAWTC 2 cut(s) 326, 485
PflMI CCANNNNNTGG 1 cut(s) 443
PfoI TCCNGGA 1 cut(s) 388
PkrI GCNGC 1 cut(s) 545
Ppu21I YACGTR 2 cut(s) 508, 567
Psp124BI GAGCTC 1 cut(s) 288
Psp1406I AACGTT 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 354
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 3 cut(s) 435, 658, 678
PspPI GGNCC 1 cut(s) 528
RsaI GTAC 3 cut(s) 25, 569, 658
RsaNI GTAC 3 cut(s) 24, 568, 657
RseI CAYNNNNRTG 1 cut(s) 575
SacI GAGCTC 1 cut(s) 288
SalI GTCGAC 1 cut(s) 511
SaqAI TTAA 2 cut(s) 630, 645
SatI GCNGC 1 cut(s) 544
Sau3AI GATC 3 cut(s) 126, 189, 295
Sau96I GGNCC 1 cut(s) 528
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 2 cut(s) 288, 436
SfaNI GCATC 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SnaBI TACGTA 1 cut(s) 567
Sse9I AATT 2 cut(s) 143, 219
SsiI CCGC 5 cut(s) 159, 231, 255, 281, 543
SspMI CTAG 2 cut(s) 429, 683
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 388
StyI CCWWGG 2 cut(s) 428, 535
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 3 cut(s) 510, 569, 645
TaqI TCGA 1 cut(s) 512
TasI AATT 2 cut(s) 143, 219
TauI GCSGC 1 cut(s) 546
TfiI GAWTC 2 cut(s) 326, 485
Tru1I TTAA 2 cut(s) 630, 645
Tru9I TTAA 2 cut(s) 630, 645
TscAI CASTG 2 cut(s) 271, 337
TseFI GTSAC 1 cut(s) 171
Tsp45I GTSAC 1 cut(s) 171
TspDTI ATGAA 1 cut(s) 498
TspRI CASTG 2 cut(s) 271, 337
Van91I CCANNNNNTGG 1 cut(s) 443
XapI RAATTY 1 cut(s) 143
XbaI TCTAGA 1 cut(s) 682
XmaJI CCTAGG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 512
XspI CTAG 2 cut(s) 429, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.