Rmu_sc0004571.1_g000006

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004571.1
OG0030897
Physical Location & Seq
Forward (+)
23080 .. 28157
5078 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1446 bp
atgctattttttcttcccgaaatcatcgttgatcagtctttgcaacctgcaattgtggaaactgattctttaatcatccaacaacagttgtgtaaggctgctgttaacctttccagattgggtcagatttatgatgatctaggagtgcttcttgatgctatgcccaatgttcgaattatgcacacaagaagggaagccaacattgcagctcacctgatggctgcagaagctgttgctgctggtcaggctctcttttactctactgtaccttcctttctttgtaatgtgatagatgttgaactttgtttttcttctgtggatgcccaaatgctattttttcttcccgaaatcatcgttgaccagtctttgcaacctgcaattgtggaaactgattctttaatcatccaacaacagttgtgtaaggctgctgttaacctttccagattgggtcagatttatgatgatctaggagtgcttcttgatgctatgcccaatgttcgaattatgcacacaagaagggaagccaacattgcagctcacctgatggctgcagaagctgttgctgctggtcaggctctcttttactcttctgtaccttcctttctttgtaatgtgatagatgttgaactttctattgggaccatggtgtttccaaatcttttagcagctaccactagaggtctaccagatgaatctctaataacaaagccacctgaagccgaggcccaacccactcccactgccatcatccctacacccagaccagttcacgtcgaaaccctagcaagtctgtcagaactgtttccacccctggttcagtcaggcggcctccccagatacggacccacggcagccatcccgtccgcccaaaacttgcctctgtcgaccctacagccgcccgatccactcacaatccaattgtctccttcggttacccaatcagccggcgtcaagcctcaccgctcaatcagcaaagaacctgagatctccgccgccctgcaactcaaagcccaaatccacaacaggcgccgcagatccagacccgacccgccttgccttcaaactcgacaccggaagaacccttgctgttgtacacgcccaacacacaccagactagatcgacgtcggaagacgtcctctagcaggcatcaggcggcctccccagatacggacccacggcagccatcccgtccgcccaaaacttgcctctgtcgacactacagccgcccgatccactcacaatccaattgtctccttcggttacccaatcatccggcgtcaagcctcaccgctcaatcagcaaagaacctgagatctccgccgccctgcaactcaaagcccaaatccacaaccggcgccgcagatccagacccgacccgccttgccttcaaactcgacaccggaagaacccttgctgttgtacacgcccaacacacaccagactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

481

Amino Acids

52.43

Weight (kDa)

8.68

Isoelectric Point (pI)

64.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 9 - 77 2.5e-06 Reverse transcriptase-like
RVT_3 PF13456 117 - 186 2.1e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030897)

Species Orthologous Gene IDs
rosa_laevigata RLG00000029864
rosa_multiflora Rmu_sc0004571.1_g000006

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1126, 1136
Acc36I ACCTGC 2 cut(s) 55, 382
AccB1I GGYRCC 2 cut(s) 1026, 1355
AccBSI CCGCTC 2 cut(s) 963, 1292
AccI GTMKAC 3 cut(s) 682, 884, 1213
AclWI GGATC 4 cut(s) 896, 1029, 1225, 1358
AcuI CTGAAG 1 cut(s) 735
AcyI GRCGYC 6 cut(s) 948, 1027, 1123, 1133, 1277, 1356
AfaI GTAC 4 cut(s) 267, 594, 1093, 1422
AfiI CCNNNNNNNGG 3 cut(s) 839, 1143, 1168
AgsI TTSAA 4 cut(s) 299, 626, 1061, 1390
AjiI CACGTC 1 cut(s) 772
AjnI CCWGG 1 cut(s) 810
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AluBI AGCT 5 cut(s) 209, 230, 536, 557, 668
AluI AGCT 5 cut(s) 209, 230, 536, 557, 668
Alw26I GTCTC 2 cut(s) 927, 1256
AlwI GGATC 4 cut(s) 896, 1029, 1225, 1358
AlwNI CAGNNNCTG 2 cut(s) 230, 557
AoxI GGCC 3 cut(s) 723, 826, 1155
Asp700I GAANNNNTTC 1 cut(s) 801
AspLEI GCGC 2 cut(s) 1029, 1358
AspS9I GGNCC 4 cut(s) 639, 724, 842, 1171
AsuHPI GGTGA 4 cut(s) 203, 530, 950, 1279
AsuII TTCGAA 2 cut(s) 172, 499
AvaII GGWCC 3 cut(s) 639, 842, 1171
BanI GGYRCC 2 cut(s) 1026, 1355
BbsI GAAGAC 1 cut(s) 1136
BccI CCATC 5 cut(s) 211, 538, 752, 863, 1192
BceAI ACGGC 2 cut(s) 864, 1193
BciT130I CCWGG 1 cut(s) 812
BclI TGATCA 1 cut(s) 31
BcoDI GTCTC 2 cut(s) 927, 1256
BfaI CTAG 7 cut(s) 140, 467, 675, 782, 1115, 1140, 1444
BfmI CTRYAG 4 cut(s) 222, 549, 890, 1219
BfoI RGCGCY 2 cut(s) 1030, 1359
BfuAI ACCTGC 2 cut(s) 55, 382
BglII AGATCT 2 cut(s) 984, 1313
Bme1390I CCNGG 1 cut(s) 812
Bme18I GGWCC 3 cut(s) 639, 842, 1171
BmgBI CACGTC 1 cut(s) 772
BmgT120I GGNCC 4 cut(s) 639, 724, 842, 1171
BmiI GGNNCC 5 cut(s) 640, 844, 1028, 1173, 1357
BmrFI CCNGG 1 cut(s) 812
BmsI GCATC 4 cut(s) 145, 310, 472, 1156
BpiI GAAGAC 1 cut(s) 1136
Bpu14I TTCGAA 2 cut(s) 172, 499
BsaHI GRCGYC 6 cut(s) 948, 1027, 1123, 1133, 1277, 1356
BsaJI CCNNGG 5 cut(s) 642, 720, 810, 846, 1175
BsaWI WCCGGW 2 cut(s) 1071, 1400
Bsc4I CCNNNNNNNGG 3 cut(s) 839, 1143, 1168
Bse118I RCCGGY 2 cut(s) 944, 1352
Bse1I ACTGG 2 cut(s) 361, 764
Bse3DI GCAATG 2 cut(s) 201, 528
BseBI CCWGG 1 cut(s) 812
BseDI CCNNGG 5 cut(s) 642, 720, 810, 846, 1175
BseGI GGATG 7 cut(s) 75, 325, 402, 747, 855, 1184, 1270
BseLI CCNNNNNNNGG 3 cut(s) 839, 1143, 1168
BseMI GCAATG 2 cut(s) 201, 528
BseMII CTCAG 2 cut(s) 972, 1301
BseNI ACTGG 2 cut(s) 361, 764
BshFI GGCC 3 cut(s) 725, 828, 1157
BshNI GGYRCC 2 cut(s) 1026, 1355
BsiSI CCGG 5 cut(s) 945, 1072, 1274, 1353, 1401
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 3 cut(s) 839, 1143, 1168
BsmAI GTCTC 2 cut(s) 927, 1256
BsmFI GGGAC 1 cut(s) 652
BsnI GGCC 3 cut(s) 725, 828, 1157
Bsp119I TTCGAA 2 cut(s) 172, 499
Bsp1407I TGTACA 2 cut(s) 1091, 1420
Bsp19I CCATGG 1 cut(s) 642
BspANI GGCC 3 cut(s) 725, 828, 1157
BspCNI CTCAG 2 cut(s) 973, 1302
BspLI GGNNCC 5 cut(s) 640, 844, 1028, 1173, 1357
BspMAI CTGCAG 2 cut(s) 226, 553
BspMI ACCTGC 2 cut(s) 55, 382
BspPI GGATC 4 cut(s) 896, 1029, 1225, 1358
BspT104I TTCGAA 2 cut(s) 172, 499
BspT107I GGYRCC 2 cut(s) 1026, 1355
BsrBI CCGCTC 2 cut(s) 963, 1292
BsrDI GCAATG 2 cut(s) 201, 528
BsrFI RCCGGY 2 cut(s) 944, 1352
BsrGI TGTACA 2 cut(s) 1091, 1420
BsrI ACTGG 2 cut(s) 361, 764
BssAI RCCGGY 2 cut(s) 944, 1352
BssECI CCNNGG 5 cut(s) 642, 720, 810, 846, 1175
BssNI GRCGYC 6 cut(s) 948, 1027, 1123, 1133, 1277, 1356
BssT1I CCWWGG 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 812
Bst4CI ACNGT 4 cut(s) 87, 265, 414, 801
Bst6I CTCTTC 1 cut(s) 592
BstACI GRCGYC 6 cut(s) 948, 1027, 1123, 1133, 1277, 1356
BstAUI TGTACA 2 cut(s) 1091, 1420
BstBI TTCGAA 2 cut(s) 172, 499
BstC8I GCNNGC 2 cut(s) 946, 1145
BstDEI CTNAG 2 cut(s) 981, 1310
BstDSI CCRYGG 3 cut(s) 642, 846, 1175
BstEII GGTNACC 2 cut(s) 931, 1260
BstENI CCTNNNNNAGG 1 cut(s) 1141
BstF5I GGATG 7 cut(s) 75, 325, 402, 747, 855, 1184, 1270
BstH2I RGCGCY 2 cut(s) 1030, 1359
BstHHI GCGC 2 cut(s) 1029, 1358
BstMAI GTCTC 2 cut(s) 927, 1256
BstNI CCWGG 1 cut(s) 812
BstPI GGTNACC 2 cut(s) 931, 1260
BstSCI CCNGG 1 cut(s) 810
BstSFI CTRYAG 4 cut(s) 222, 549, 890, 1219
BstV2I GAAGAC 1 cut(s) 1136
BstX2I RGATCY 4 cut(s) 984, 1034, 1313, 1363
BstYI RGATCY 4 cut(s) 984, 1034, 1313, 1363
BsuRI GGCC 3 cut(s) 725, 828, 1157
BtgI CCRYGG 3 cut(s) 642, 846, 1175
BtrI CACGTC 1 cut(s) 772
BtsCI GGATG 7 cut(s) 75, 325, 402, 747, 855, 1184, 1270
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 1 cut(s) 738
BveI ACCTGC 2 cut(s) 55, 382
Cac8I GCNNGC 2 cut(s) 946, 1145
CaiI CAGNNNCTG 2 cut(s) 230, 557
CfoI GCGC 2 cut(s) 1029, 1358
Cfr10I RCCGGY 2 cut(s) 944, 1352
Cfr13I GGNCC 4 cut(s) 639, 724, 842, 1171
CseI GACGC 2 cut(s) 937, 1266
Csp6I GTAC 4 cut(s) 266, 593, 1092, 1421
CviAII CATG 1 cut(s) 643
CviQI GTAC 4 cut(s) 266, 593, 1092, 1421
DdeI CTNAG 2 cut(s) 981, 1310
DinI GGCGCC 2 cut(s) 1028, 1357
Eam1104I CTCTTC 1 cut(s) 592
EarI CTCTTC 1 cut(s) 592
EciI GGCGGA 4 cut(s) 853, 979, 1182, 1308
Eco130I CCWWGG 1 cut(s) 642
Eco47I GGWCC 3 cut(s) 639, 842, 1171
Eco57I CTGAAG 1 cut(s) 735
Eco91I GGTNACC 2 cut(s) 931, 1260
EcoNI CCTNNNNNAGG 1 cut(s) 1141
EcoO65I GGTNACC 2 cut(s) 931, 1260
EcoRII CCWGG 1 cut(s) 810
EcoT14I CCWWGG 1 cut(s) 642
EgeI GGCGCC 2 cut(s) 1028, 1357
EheI GGCGCC 2 cut(s) 1028, 1357
ErhI CCWWGG 1 cut(s) 642
FaeI CATG 1 cut(s) 646
FaiI YATR 7 cut(s) 132, 161, 179, 459, 488, 506, 644
FaqI GGGAC 1 cut(s) 652
FatI CATG 1 cut(s) 642
FauI CCCGC 2 cut(s) 1056, 1385
FbaI TGATCA 1 cut(s) 31
FblI GTMKAC 3 cut(s) 682, 884, 1213
FokI GGATG 7 cut(s) 62, 332, 389, 734, 842, 1171, 1257
FspBI CTAG 7 cut(s) 140, 467, 675, 782, 1115, 1140, 1444
GlaI GCGC 2 cut(s) 1028, 1357
HaeII RGCGCY 2 cut(s) 1030, 1359
HaeIII GGCC 3 cut(s) 725, 828, 1157
HapII CCGG 5 cut(s) 945, 1072, 1274, 1353, 1401
HgaI GACGC 2 cut(s) 937, 1266
HhaI GCGC 2 cut(s) 1029, 1358
Hin1I GRCGYC 6 cut(s) 948, 1027, 1123, 1133, 1277, 1356
Hin1II CATG 1 cut(s) 646
Hin6I GCGC 2 cut(s) 1027, 1356
HinP1I GCGC 2 cut(s) 1027, 1356
HincII GTYRAC 5 cut(s) 106, 358, 433, 885, 1214
HindII GTYRAC 5 cut(s) 106, 358, 433, 885, 1214
HinfI GANTC 3 cut(s) 65, 392, 692
HpaI GTTAAC 2 cut(s) 106, 433
HpaII CCGG 5 cut(s) 945, 1072, 1274, 1353, 1401
HphI GGTGA 4 cut(s) 203, 530, 950, 1279
Hpy166II GTNNAC 9 cut(s) 106, 358, 433, 683, 769, 885, 1094, 1214, 1423
Hpy188I TCNGA 4 cut(s) 126, 453, 796, 1128
Hpy188III TCNNGA 8 cut(s) 17, 114, 152, 344, 441, 479, 1038, 1367
Hpy8I GTNNAC 9 cut(s) 106, 358, 433, 683, 769, 885, 1094, 1214, 1423
Hpy99I CGWCG 3 cut(s) 776, 1125, 1128
HpyAV CCTTC 8 cut(s) 183, 279, 510, 606, 936, 1067, 1265, 1396
HpyCH4III ACNGT 4 cut(s) 87, 265, 414, 801
HpyCH4IV ACGT 3 cut(s) 771, 1123, 1133
HpyF3I CTNAG 2 cut(s) 981, 1310
HpySE526I ACGT 3 cut(s) 771, 1123, 1133
Hsp92I GRCGYC 6 cut(s) 948, 1027, 1123, 1133, 1277, 1356
Hsp92II CATG 1 cut(s) 646
HspAI GCGC 2 cut(s) 1027, 1356
KasI GGCGCC 2 cut(s) 1026, 1355
KroI GCCGGC 1 cut(s) 944
KroNI GCCGGC 1 cut(s) 946
Ksp22I TGATCA 1 cut(s) 31
KspAI GTTAAC 2 cut(s) 106, 433
LweI GCATC 4 cut(s) 145, 310, 472, 1156
MaeI CTAG 7 cut(s) 140, 467, 675, 782, 1115, 1140, 1444
MaeII ACGT 3 cut(s) 771, 1123, 1133
MaeIII GTNAC 2 cut(s) 931, 1260
MbiI CCGCTC 2 cut(s) 963, 1292
MboII GAAGA 7 cut(s) 5, 303, 332, 579, 1087, 1141, 1416
MfeI CAATTG 4 cut(s) 51, 378, 917, 1246
MflI RGATCY 4 cut(s) 984, 1034, 1313, 1363
MluCI AATT 6 cut(s) 51, 174, 378, 501, 917, 1246
Mly113I GGCGCC 2 cut(s) 1027, 1356
MmeI TCCRAC 3 cut(s) 103, 430, 1106
MnlI CCTC 9 cut(s) 671, 715, 839, 888, 966, 1147, 1168, 1217, 1295
MroNI GCCGGC 1 cut(s) 944
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 4 cut(s) 71, 105, 398, 432
MspI CCGG 5 cut(s) 945, 1072, 1274, 1353, 1401
MspR9I CCNGG 1 cut(s) 812
MunI CAATTG 4 cut(s) 51, 378, 917, 1246
MvaI CCWGG 1 cut(s) 812
NaeI GCCGGC 1 cut(s) 946
NarI GGCGCC 2 cut(s) 1027, 1356
NcoI CCATGG 1 cut(s) 642
NgoMIV GCCGGC 1 cut(s) 944
NlaIII CATG 1 cut(s) 646
NlaIV GGNNCC 5 cut(s) 640, 844, 1028, 1173, 1357
NmeAIII GCCGAG 1 cut(s) 745
NspV TTCGAA 2 cut(s) 172, 499
PdiI GCCGGC 1 cut(s) 946
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 3 cut(s) 65, 392, 692
PluTI GGCGCC 2 cut(s) 1030, 1359
Psp6I CCWGG 1 cut(s) 810
PspEI GGTNACC 2 cut(s) 931, 1260
PspGI CCWGG 1 cut(s) 810
PspN4I GGNNCC 5 cut(s) 640, 844, 1028, 1173, 1357
PspPI GGNCC 4 cut(s) 639, 724, 842, 1171
PstI CTGCAG 2 cut(s) 226, 553
PstNI CAGNNNCTG 2 cut(s) 230, 557
PsuI RGATCY 4 cut(s) 984, 1034, 1313, 1363
RsaI GTAC 4 cut(s) 267, 594, 1093, 1422
RsaNI GTAC 4 cut(s) 266, 593, 1092, 1421
SalI GTCGAC 2 cut(s) 883, 1212
SaqAI TTAA 4 cut(s) 71, 105, 398, 432
Sau96I GGNCC 4 cut(s) 639, 724, 842, 1171
ScrFI CCNGG 1 cut(s) 812
SfaNI GCATC 4 cut(s) 145, 310, 472, 1156
SfcI CTRYAG 4 cut(s) 222, 549, 890, 1219
SfoI GGCGCC 2 cut(s) 1028, 1357
SfuI TTCGAA 2 cut(s) 172, 499
SinI GGWCC 3 cut(s) 639, 842, 1171
Sse9I AATT 6 cut(s) 51, 174, 378, 501, 917, 1246
SspDI GGCGCC 2 cut(s) 1026, 1355
SspMI CTAG 7 cut(s) 140, 467, 675, 782, 1115, 1140, 1444
StyD4I CCNGG 1 cut(s) 810
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 4 cut(s) 87, 265, 414, 801
TaiI ACGT 3 cut(s) 774, 1126, 1136
TaqI TCGA 8 cut(s) 172, 499, 774, 884, 1066, 1120, 1213, 1395
TasI AATT 6 cut(s) 51, 174, 378, 501, 917, 1246
TatI WGTACW 2 cut(s) 1091, 1420
TauI GCSGC 8 cut(s) 828, 898, 995, 1032, 1157, 1227, 1324, 1361
TfiI GAWTC 3 cut(s) 65, 392, 692
Tru1I TTAA 4 cut(s) 71, 105, 398, 432
Tru9I TTAA 4 cut(s) 71, 105, 398, 432
TscAI CASTG 1 cut(s) 745
TspDTI ATGAA 1 cut(s) 705
TspGWI ACGGA 2 cut(s) 855, 1184
TspRI CASTG 1 cut(s) 745
VpaK11BI GGWCC 3 cut(s) 639, 842, 1171
XagI CCTNNNNNAGG 1 cut(s) 1141
XmiI GTMKAC 3 cut(s) 682, 884, 1213
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 7 cut(s) 140, 467, 675, 782, 1115, 1140, 1444
ZraI GACGTC 2 cut(s) 1124, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.