Rmu_sc0004757.1_g000019

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004757.1
OG0031318
Physical Location & Seq
Forward (+)
84479 .. 91986
7508 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1464 bp
atgaggacgacttggttctccgtttgcctcccccaagaagctgaaactcatgcttggggaccttggtgtgggcaccaatggctcaccggcacctctaagaaagtgaaactaccagtaactccaatcaagttctctgctagatggttggacctggttgagagatcgggagggatgcttgttccagcagaggagagcaccaacctcgccacagggttcttctctaacctttcagaaaaggacttttgccttacggataaggaatttgggtgtgatctcttcggtcaccaaatcgatactcagcattggcgttacccttgcttcgctggtagtgtctgtggcagtagcactgtcagttggctcagagagactaggactaagagggaggggagcggcaggttggtgccgtgtacgattcctatttctcggaggaaactggatccaacttggtttttatcggataagaggattgcaatgaaggttggtggcggtgtggtgtttggtttcatatcttgtgcagccacgtactgggtgtttctcctcacttgtcgactatggggatggaggtttccaaggcaacgaatctactacttgggctccttatctaaggatggtgatgctcaacatggtggctatttaggctatgctggtgatggcttgaaggaagttagtaggggcgacctatcttctggcgatggcaacatacaacttggtagcggatacgttgtggagcagttgtttgctagggtttcagaggatttgggccattgggctcatgtattgggcttaatctggactgggatgaattggggcgatgaaggtgatgtgatagattgcattgacatctataagcagcctgctcttagtcatccacttctcaaaaatcatactatacagctgaatccgccaagtccctatccaagtggacttaatgaaacagaaagttctcatcaagatgacgagcaagtactgaaatcttggttattgaatgaagagtgcccagaaggaacaatccccattcttcgaactcaggcttttaatagttcttttcctaaatctagcccacctttcaatggaagcaacgttgagttcacgcctgcatctggtcatgagtacgccgtagctagtctttcaggtggccaatattacggagcacacgcatctgtgaacgtatggagccctttatcagtttttgaggagaatagtatatcccagatatgggtagcgggaggtcatgaacaagcacgaagtactgttgaagctggagagagaagtcatcctccaccttgctgtgctccggtgatcagtggccgaccgctgtctccggcaaccggtgaccggacttcggggaccggtgacgggaatccggcaaccgatgaccagaatcccgcgatcggtcaccggaatcctgaatccggctatcggactggtgactggattccggtgtctgaatttattgtcccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

487

Amino Acids

53.5

Weight (kDa)

5.68

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 272 - 344 1.1e-21 Neprosin activation peptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031318)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004757.1_g000019 Rmu_sc0009946.1_g000007

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 384
AccB1I GGYRCC 3 cut(s) 72, 89, 400
AccB7I CCANNNNNTGG 1 cut(s) 525
AccBSI CCGCTC 1 cut(s) 390
AccI GTMKAC 1 cut(s) 547
AccII CGCG 1 cut(s) 1388
AciI CCGC 7 cut(s) 390, 486, 714, 902, 1223, 1313, 1386
AclI AACGTT 1 cut(s) 1080
AclWI GGATC 2 cut(s) 431, 444
AcoI YGGCCR 2 cut(s) 1135, 1306
AcsI RAATTY 2 cut(s) 260, 1448
AfaI GTAC 5 cut(s) 409, 524, 966, 1112, 1249
AgeI ACCGGT 2 cut(s) 1328, 1349
AgsI TTSAA 4 cut(s) 658, 985, 1069, 1256
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 4 cut(s) 41, 895, 1121, 1259
AluI AGCT 4 cut(s) 41, 895, 1121, 1259
Alw21I GWGCWC 3 cut(s) 197, 1153, 1294
Alw26I GTCTC 2 cut(s) 359, 1323
AlwI GGATC 2 cut(s) 431, 444
AoxI GGCC 3 cut(s) 760, 1135, 1306
ApeKI GCWGC 2 cut(s) 515, 850
ApoI RAATTY 2 cut(s) 260, 1448
AsiGI ACCGGT 2 cut(s) 1328, 1349
AspS9I GGNCC 4 cut(s) 59, 148, 760, 1347
AsuII TTCGAA 1 cut(s) 1021
AvaII GGWCC 3 cut(s) 59, 148, 1347
BaeGI GKGCMC 2 cut(s) 75, 998
BalI TGGCCA 1 cut(s) 1137
BamHI GGATCC 1 cut(s) 436
BanI GGYRCC 3 cut(s) 72, 89, 400
BanII GRGCYC 3 cut(s) 596, 772, 1178
Bbv12I GWGCWC 3 cut(s) 197, 1153, 1294
BbvI GCAGC 2 cut(s) 527, 862
BccI CCATC 5 cut(s) 135, 552, 602, 644, 686
BceAI ACGGC 2 cut(s) 388, 1100
BcgI CGANNNNNNTGC 2 cut(s) 184, 218
BciT130I CCWGG 1 cut(s) 152
BciVI GTATCC 1 cut(s) 710
BclI TGATCA 1 cut(s) 1299
BcoDI GTCTC 2 cut(s) 359, 1323
BfaI CTAG 5 cut(s) 138, 369, 741, 1056, 1122
BfuAI ACCTGC 1 cut(s) 384
BfuI GTATCC 1 cut(s) 710
BisI GCNGC 3 cut(s) 391, 516, 851
BlsI GCNGC 3 cut(s) 392, 517, 852
BmcAI AGTACT 2 cut(s) 966, 1249
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 3 cut(s) 59, 148, 1347
BmgT120I GGNCC 4 cut(s) 59, 148, 760, 1347
BmiI GGNNCC 8 cut(s) 60, 74, 91, 402, 438, 595, 1175, 1348
BmrFI CCNGG 1 cut(s) 152
BmrI ACTGGG 2 cut(s) 535, 804
BmsI GCATC 4 cut(s) 162, 604, 1106, 1166
BmuI ACTGGG 2 cut(s) 535, 804
BoxI GACNNNNGTC 1 cut(s) 1315
BpmI CTGGAG 1 cut(s) 1281
Bpu14I TTCGAA 1 cut(s) 1021
Bsa29I ATCGAT 1 cut(s) 291
BsaAI YACGTR 1 cut(s) 522
BsaJI CCNNGG 2 cut(s) 62, 569
BsaWI WCCGGW 6 cut(s) 1294, 1328, 1335, 1349, 1398, 1438
BsaXI ACNNNNNCTCC 4 cut(s) 578, 608, 719, 749
Bse118I RCCGGY 3 cut(s) 86, 1328, 1349
Bse1I ACTGG 6 cut(s) 113, 438, 530, 799, 1429, 1436
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 1 cut(s) 152
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 62, 569
BseGI GGATG 6 cut(s) 177, 563, 613, 804, 865, 1273
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 3 cut(s) 311, 373, 1040
BseNI ACTGG 6 cut(s) 113, 438, 530, 799, 1429, 1436
BseRI GAGGAG 3 cut(s) 203, 527, 1208
BseSI GKGCMC 2 cut(s) 75, 998
BseXI GCAGC 2 cut(s) 527, 862
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 1388
Bsh1285I CGRYCG 2 cut(s) 1313, 1392
BshFI GGCC 3 cut(s) 762, 1137, 1308
BshNI GGYRCC 3 cut(s) 72, 89, 400
BshTI ACCGGT 2 cut(s) 1328, 1349
BshVI ATCGAT 1 cut(s) 291
BsiEI CGRYCG 2 cut(s) 1313, 1392
BsiHKAI GWGCWC 3 cut(s) 197, 1153, 1294
BslFI GGGAC 4 cut(s) 72, 894, 1360, 1442
BsmAI GTCTC 2 cut(s) 359, 1323
BsmFI GGGAC 4 cut(s) 72, 894, 1360, 1442
BsnI GGCC 3 cut(s) 762, 1137, 1308
Bsp119I TTCGAA 1 cut(s) 1021
Bsp1286I GDGCHC 8 cut(s) 75, 197, 596, 772, 998, 1153, 1178, 1294
Bsp143I GATC 5 cut(s) 161, 271, 436, 1299, 1389
BspACI CCGC 7 cut(s) 390, 486, 714, 902, 1223, 1313, 1386
BspANI GGCC 3 cut(s) 762, 1137, 1308
BspCNI CTCAG 3 cut(s) 310, 372, 1039
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 1388
BspHI TCATGA 2 cut(s) 1105, 1231
BspLI GGNNCC 8 cut(s) 60, 74, 91, 402, 438, 595, 1175, 1348
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 431, 444
BspT104I TTCGAA 1 cut(s) 1021
BspT107I GGYRCC 3 cut(s) 72, 89, 400
BsrBI CCGCTC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 3 cut(s) 86, 1328, 1349
BsrI ACTGG 6 cut(s) 113, 438, 530, 799, 1429, 1436
BssAI RCCGGY 3 cut(s) 86, 1328, 1349
BssECI CCNNGG 2 cut(s) 62, 569
BssMI GATC 5 cut(s) 161, 271, 436, 1299, 1389
BssT1I CCWWGG 2 cut(s) 62, 569
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 349, 1252
Bst6I CTCTTC 2 cut(s) 281, 984
BstBAI YACGTR 1 cut(s) 522
BstBI TTCGAA 1 cut(s) 1021
BstC8I GCNNGC 2 cut(s) 855, 1095
BstDEI CTNAG 7 cut(s) 96, 297, 359, 375, 603, 860, 1026
BstEII GGTNACC 3 cut(s) 281, 1331, 1394
BstF5I GGATG 6 cut(s) 177, 563, 613, 804, 865, 1273
BstFNI CGCG 1 cut(s) 1388
BstKTI GATC 5 cut(s) 164, 274, 439, 1302, 1392
BstMAI GTCTC 2 cut(s) 359, 1323
BstMBI GATC 5 cut(s) 161, 271, 436, 1299, 1389
BstMCI CGRYCG 2 cut(s) 1313, 1392
BstMWI GCNNNNNNNGC 3 cut(s) 79, 636, 901
BstNI CCWGG 1 cut(s) 152
BstPAI GACNNNNGTC 1 cut(s) 1315
BstPI GGTNACC 3 cut(s) 281, 1331, 1394
BstSCI CCNGG 1 cut(s) 150
BstSLI GKGCMC 2 cut(s) 75, 998
BstUI CGCG 1 cut(s) 1388
BstV1I GCAGC 2 cut(s) 527, 862
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
Bsu15I ATCGAT 1 cut(s) 291
BsuI GTATCC 1 cut(s) 710
BsuRI GGCC 3 cut(s) 762, 1137, 1308
BsuTUI ATCGAT 1 cut(s) 291
BtgZI GCGATG 2 cut(s) 705, 825
BtsCI GGATG 6 cut(s) 177, 563, 613, 804, 865, 1273
BtsIMutI CAGTG 2 cut(s) 345, 1309
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 855, 1095
CciI TCATGA 2 cut(s) 1105, 1231
Cfr10I RCCGGY 3 cut(s) 86, 1328, 1349
Cfr13I GGNCC 4 cut(s) 59, 148, 760, 1347
ClaI ATCGAT 1 cut(s) 291
CsiI ACCWGGT 1 cut(s) 150
Csp6I GTAC 5 cut(s) 408, 523, 965, 1111, 1248
CspAI ACCGGT 2 cut(s) 1328, 1349
CviAII CATG 5 cut(s) 50, 623, 773, 1106, 1232
CviQI GTAC 5 cut(s) 408, 523, 965, 1111, 1248
DdeI CTNAG 7 cut(s) 96, 297, 359, 375, 603, 860, 1026
DpnI GATC 5 cut(s) 163, 273, 438, 1301, 1391
DpnII GATC 5 cut(s) 161, 271, 436, 1299, 1389
EaeI YGGCCR 2 cut(s) 1135, 1306
Eam1104I CTCTTC 2 cut(s) 281, 984
EarI CTCTTC 2 cut(s) 281, 984
EciI GGCGGA 1 cut(s) 891
Eco130I CCWWGG 2 cut(s) 62, 569
Eco24I GRGCYC 3 cut(s) 596, 772, 1178
Eco47I GGWCC 3 cut(s) 59, 148, 1347
Eco91I GGTNACC 3 cut(s) 281, 1331, 1394
EcoO109I RGGNCCY 1 cut(s) 59
EcoO65I GGTNACC 3 cut(s) 281, 1331, 1394
EcoRII CCWGG 1 cut(s) 150
EcoT14I CCWWGG 2 cut(s) 62, 569
EcoT38I GRGCYC 3 cut(s) 596, 772, 1178
ErhI CCWWGG 2 cut(s) 62, 569
FaeI CATG 5 cut(s) 53, 626, 776, 1109, 1235
FaqI GGGAC 4 cut(s) 72, 894, 1360, 1442
FatI CATG 5 cut(s) 49, 622, 772, 1105, 1231
FauI CCCGC 2 cut(s) 1216, 1393
FbaI TGATCA 1 cut(s) 1299
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 391, 516, 851
FokI GGATG 6 cut(s) 184, 570, 620, 811, 852, 1260
FriOI GRGCYC 3 cut(s) 596, 772, 1178
Fsp4HI GCNGC 3 cut(s) 391, 516, 851
FspBI CTAG 5 cut(s) 138, 369, 741, 1056, 1122
GluI GCNGC 3 cut(s) 391, 516, 851
GsuI CTGGAG 1 cut(s) 1281
HaeIII GGCC 3 cut(s) 762, 1137, 1308
Hin1II CATG 5 cut(s) 53, 626, 776, 1109, 1235
HincII GTYRAC 1 cut(s) 548
HindII GTYRAC 1 cut(s) 548
HinfI GANTC 8 cut(s) 412, 579, 898, 1360, 1381, 1402, 1409, 1435
Hpy166II GTNNAC 5 cut(s) 408, 548, 923, 1089, 1165
Hpy188I TCNGA 7 cut(s) 232, 362, 426, 457, 751, 1422, 1447
Hpy188III TCNNGA 6 cut(s) 165, 790, 950, 1106, 1232, 1406
Hpy8I GTNNAC 5 cut(s) 408, 548, 923, 1089, 1165
HpyAV CCTTC 4 cut(s) 469, 652, 809, 995
HpyCH4III ACNGT 2 cut(s) 349, 1252
HpyCH4IV ACGT 4 cut(s) 521, 720, 1080, 1167
HpyCH4V TGCA 4 cut(s) 470, 515, 834, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 636, 901
HpyF3I CTNAG 7 cut(s) 96, 297, 359, 375, 603, 860, 1026
HpySE526I ACGT 4 cut(s) 521, 720, 1080, 1167
Hsp92II CATG 5 cut(s) 53, 626, 776, 1109, 1235
Ksp22I TGATCA 1 cut(s) 1299
Kzo9I GATC 5 cut(s) 161, 271, 436, 1299, 1389
LmnI GCTCC 6 cut(s) 387, 599, 727, 1148, 1173, 1297
Lsp1109I GCAGC 2 cut(s) 527, 862
LweI GCATC 4 cut(s) 162, 604, 1106, 1166
MabI ACCWGGT 1 cut(s) 150
MaeI CTAG 5 cut(s) 138, 369, 741, 1056, 1122
MaeII ACGT 4 cut(s) 521, 720, 1080, 1167
MaeIII GTNAC 7 cut(s) 115, 281, 308, 1331, 1352, 1394, 1427
MalI GATC 5 cut(s) 163, 273, 438, 1301, 1391
MbiI CCGCTC 1 cut(s) 390
MboI GATC 5 cut(s) 161, 271, 436, 1299, 1389
MboII GAAGA 5 cut(s) 208, 268, 675, 1001, 1010
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 8 cut(s) 75, 197, 596, 772, 998, 1153, 1178, 1294
MlsI TGGCCA 1 cut(s) 1137
MluCI AATT 3 cut(s) 260, 802, 1448
MluNI TGGCCA 1 cut(s) 1137
MmeI TCCRAC 2 cut(s) 126, 464
Mox20I TGGCCA 1 cut(s) 1137
MscI TGGCCA 1 cut(s) 1137
MseI TTAA 3 cut(s) 785, 927, 1035
MslI CAYNNNNRTG 1 cut(s) 951
Msp20I TGGCCA 1 cut(s) 1137
MspA1I CMGCKG 2 cut(s) 895, 1315
MspR9I CCNGG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 152
MvnI CGCG 1 cut(s) 1388
MwoI GCNNNNNNNGC 3 cut(s) 79, 636, 901
NdeII GATC 5 cut(s) 161, 271, 436, 1299, 1389
NlaIII CATG 5 cut(s) 53, 626, 776, 1109, 1235
NlaIV GGNNCC 8 cut(s) 60, 74, 91, 402, 438, 595, 1175, 1348
NmuCI GTSAC 5 cut(s) 281, 1331, 1352, 1394, 1427
NspV TTCGAA 1 cut(s) 1021
PagI TCATGA 2 cut(s) 1105, 1231
PfeI GAWTC 8 cut(s) 412, 579, 898, 1360, 1381, 1402, 1409, 1435
PflMI CCANNNNNTGG 1 cut(s) 525
PinAI ACCGGT 2 cut(s) 1328, 1349
PkrI GCNGC 3 cut(s) 392, 517, 852
Ple19I CGATCG 1 cut(s) 1392
Ppu21I YACGTR 1 cut(s) 522
PpuMI RGGWCCY 1 cut(s) 59
PshAI GACNNNNGTC 1 cut(s) 1315
Psp1406I AACGTT 1 cut(s) 1080
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 150
PspEI GGTNACC 3 cut(s) 281, 1331, 1394
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 8 cut(s) 60, 74, 91, 402, 438, 595, 1175, 1348
PspPI GGNCC 4 cut(s) 59, 148, 760, 1347
PspPPI RGGWCCY 1 cut(s) 59
PsuI RGATCY 1 cut(s) 436
PvuI CGATCG 1 cut(s) 1392
PvuII CAGCTG 1 cut(s) 895
RsaI GTAC 5 cut(s) 409, 524, 966, 1112, 1249
RsaNI GTAC 5 cut(s) 408, 523, 965, 1111, 1248
RseI CAYNNNNRTG 1 cut(s) 951
SalI GTCGAC 1 cut(s) 546
SaqAI TTAA 3 cut(s) 785, 927, 1035
SatI GCNGC 3 cut(s) 391, 516, 851
Sau3AI GATC 5 cut(s) 161, 271, 436, 1299, 1389
Sau96I GGNCC 4 cut(s) 59, 148, 760, 1347
ScaI AGTACT 2 cut(s) 966, 1249
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 8 cut(s) 75, 197, 596, 772, 998, 1153, 1178, 1294
SexAI ACCWGGT 1 cut(s) 150
SfaNI GCATC 4 cut(s) 162, 604, 1106, 1166
SfuI TTCGAA 1 cut(s) 1021
SinI GGWCC 3 cut(s) 59, 148, 1347
SmiMI CAYNNNNRTG 1 cut(s) 951
Sse9I AATT 3 cut(s) 260, 802, 1448
SsiI CCGC 7 cut(s) 390, 486, 714, 902, 1223, 1313, 1386
SspI AATATT 1 cut(s) 1142
SspMI CTAG 5 cut(s) 138, 369, 741, 1056, 1122
StyD4I CCNGG 1 cut(s) 150
StyI CCWWGG 2 cut(s) 62, 569
TaaI ACNGT 2 cut(s) 349, 1252
TaiI ACGT 4 cut(s) 524, 723, 1083, 1170
TaqI TCGA 3 cut(s) 291, 547, 1021
TaqII GACCGA 2 cut(s) 269, 1382
TasI AATT 3 cut(s) 260, 802, 1448
TatI WGTACW 2 cut(s) 964, 1247
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 8 cut(s) 412, 579, 898, 1360, 1381, 1402, 1409, 1435
Tru1I TTAA 3 cut(s) 785, 927, 1035
Tru9I TTAA 3 cut(s) 785, 927, 1035
TscAI CASTG 2 cut(s) 352, 1309
TseFI GTSAC 5 cut(s) 281, 1331, 1352, 1394, 1427
TseI GCWGC 2 cut(s) 515, 850
Tsp45I GTSAC 5 cut(s) 281, 1331, 1352, 1394, 1427
TspDTI ATGAA 7 cut(s) 488, 493, 815, 828, 945, 1002, 1248
TspGWI ACGGA 3 cut(s) 10, 266, 1161
TspRI CASTG 2 cut(s) 352, 1309
Van91I CCANNNNNTGG 1 cut(s) 525
VpaK11BI GGWCC 3 cut(s) 59, 148, 1347
XapI RAATTY 2 cut(s) 260, 1448
XmiI GTMKAC 1 cut(s) 547
XspI CTAG 5 cut(s) 138, 369, 741, 1056, 1122
ZrmI AGTACT 2 cut(s) 966, 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.