Rmu_sc0005631.1_g000002

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005631.1
OG0031346
Physical Location & Seq
Forward (+)
4406 .. 5715
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
atggttggccgcttgctagggccaaaagctcggtttcccggctttaaaggaacgatctcctacatatggcatatcaaatcgggtctcacgatccaggatgtgggtgatcacttcttgttccagtttgacggtgaagctgatcgcaaccgtgctctccacggtggaccttggttttataggaataccatgctcatggtaggagattttgatgggatgggttcagtggaggcagtccctctgtggagtttggagacatgggtggcggtaagaggtttgctggtggctcttcgaaacaagactgttttaaacctaattggatcaaacattggtcaggtgatccgttttgatcaaacggctctctgccgcaaggaggaggagcaaagaattcggttgacattggatactcgtcgtcgagtttgtacctggatgatgtttgaattctctgccacaattgaacttgagatcactctgatctatgaaaaggagcagtcgcttcaagggccgcaggctgctgcaatggccaatctgtcactggaggggctacgtatagactctgttttgggtgaacgtctgatccagaaggaggggactgcggaaggtatggagttggtggcatctccagagttggagggtgtacatgggatcatgcggatgtggaagaccatgcattactttccaaccaaacgagtgcctaaggtgattaccttgtatcatgtcccggcaactaagaataagatgtctataggtttgcaggtttcagagttggttctcgtgggctcaaaacgcaaggcagcgtttgagcttgctagggaaaggaaaaggtatttagctctcccttcttctgaattgcaagtcgatggaaatactactctgatgctgcatcacaagaatggtaaaatcattgtatcccccaagaagaagaaacttgggcaccctcgtggtattggagttgttgagagggccattgagacagagatttctgaggcggatggtggagagaaggaagtggtcatggaaggggaatctactccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

344

Amino Acids

38.61

Weight (kDa)

9.07

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 2 - 66 7.1e-09 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0031346)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005631.1_g000002 Rmu_sc0035580.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 742
AccB1I GGYRCC 1 cut(s) 930
AccB7I CCANNNNNTGG 1 cut(s) 100
AciI CCGC 7 cut(s) 10, 263, 364, 503, 593, 649, 986
AclWI GGATC 5 cut(s) 85, 325, 331, 568, 650
AcoI YGGCCR 2 cut(s) 7, 519
AcsI RAATTY 2 cut(s) 384, 437
AfaI GTAC 2 cut(s) 421, 636
AfiI CCNNNNNNNGG 4 cut(s) 66, 100, 370, 583
AgsI TTSAA 3 cut(s) 437, 455, 497
AjnI CCWGG 2 cut(s) 93, 422
AleI CACNNNNGTG 1 cut(s) 936
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
AluBI AGCT 4 cut(s) 29, 137, 802, 830
AluI AGCT 4 cut(s) 29, 137, 802, 830
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 3 cut(s) 89, 245, 962
AlwI GGATC 5 cut(s) 85, 325, 331, 568, 650
AoxI GGCC 5 cut(s) 7, 20, 500, 519, 960
ApeKI GCWGC 4 cut(s) 509, 512, 791, 877
ApoI RAATTY 2 cut(s) 384, 437
AspS9I GGNCC 4 cut(s) 20, 164, 500, 960
AsuC2I CCSGG 2 cut(s) 39, 719
AsuHPI GGTGA 5 cut(s) 116, 143, 346, 575, 709
AsuII TTCGAA 1 cut(s) 289
AvaII GGWCC 1 cut(s) 164
AxyI CCTNAGG 1 cut(s) 693
BaeGI GKGCMC 1 cut(s) 933
BalI TGGCCA 1 cut(s) 521
BanI GGYRCC 1 cut(s) 930
BanII GRGCYC 1 cut(s) 779
BauI CACGAG 2 cut(s) 770, 936
BbsI GAAGAC 1 cut(s) 665
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 4 cut(s) 496, 499, 803, 864
BccI CCATC 4 cut(s) 203, 208, 851, 983
BceAI ACGGC 1 cut(s) 369
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 2 cut(s) 95, 424
BciVI GTATCC 2 cut(s) 394, 916
BclI TGATCA 2 cut(s) 106, 346
BcnI CCSGG 2 cut(s) 39, 719
BcoDI GTCTC 3 cut(s) 89, 245, 962
BfaI CTAG 2 cut(s) 17, 807
BfmI CTRYAG 1 cut(s) 741
BfuAI ACCTGC 1 cut(s) 742
BfuI GTATCC 2 cut(s) 394, 916
BisI GCNGC 7 cut(s) 10, 364, 503, 510, 513, 792, 878
BlsI GCNGC 7 cut(s) 11, 365, 504, 511, 514, 793, 879
Bme1390I CCNGG 4 cut(s) 39, 95, 424, 719
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 4 cut(s) 20, 164, 500, 960
BmiI GGNNCC 1 cut(s) 932
BmrFI CCNGG 4 cut(s) 39, 95, 424, 719
BmsI GCATC 3 cut(s) 623, 864, 889
BpiI GAAGAC 1 cut(s) 665
BpmI CTGGAG 2 cut(s) 554, 603
Bpu14I TTCGAA 1 cut(s) 289
BpuEI CTTGAG 1 cut(s) 479
BpuMI CCSGG 2 cut(s) 39, 719
BsaAI YACGTR 1 cut(s) 545
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 157, 167
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 100, 370, 583
Bse1I ACTGG 2 cut(s) 121, 537
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 2 cut(s) 95, 424
BseDI CCNNGG 2 cut(s) 157, 167
BseGI GGATG 5 cut(s) 103, 219, 432, 657, 994
BseLI CCNNNNNNNGG 4 cut(s) 66, 100, 370, 583
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 1 cut(s) 972
BseNI ACTGG 2 cut(s) 121, 537
BseRI GAGGAG 2 cut(s) 386, 389
BseSI GKGCMC 1 cut(s) 933
BseXI GCAGC 4 cut(s) 496, 499, 803, 864
BshFI GGCC 5 cut(s) 9, 22, 502, 521, 962
BshNI GGYRCC 1 cut(s) 930
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 2 cut(s) 39, 719
BslFI GGGAC 3 cut(s) 218, 601, 701
BslI CCNNNNNNNGG 4 cut(s) 66, 100, 370, 583
BsmAI GTCTC 3 cut(s) 89, 245, 962
BsmFI GGGAC 3 cut(s) 218, 601, 701
BsnI GGCC 5 cut(s) 9, 22, 502, 521, 962
Bso31I GGTCTC 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 289
Bsp1286I GDGCHC 3 cut(s) 154, 779, 933
Bsp1407I TGTACA 1 cut(s) 634
BspACI CCGC 7 cut(s) 10, 263, 364, 503, 593, 649, 986
BspANI GGCC 5 cut(s) 9, 22, 502, 521, 962
BspCNI CTCAG 1 cut(s) 973
BspLI GGNNCC 1 cut(s) 932
BspMI ACCTGC 1 cut(s) 742
BspPI GGATC 5 cut(s) 85, 325, 331, 568, 650
BspQI GCTCTTC 1 cut(s) 291
BspT104I TTCGAA 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 930
BspTNI GGTCTC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 522
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 2 cut(s) 121, 537
BssECI CCNNGG 2 cut(s) 157, 167
BssSI CACGAG 2 cut(s) 770, 936
BssT1I CCWWGG 1 cut(s) 167
Bst2BI CACGAG 2 cut(s) 770, 936
Bst2UI CCWGG 2 cut(s) 95, 424
Bst4CI ACNGT 4 cut(s) 131, 149, 161, 301
Bst6I CTCTTC 1 cut(s) 291
BstAUI TGTACA 1 cut(s) 634
BstBAI YACGTR 1 cut(s) 545
BstBI TTCGAA 1 cut(s) 289
BstC8I GCNNGC 3 cut(s) 14, 507, 804
BstDEI CTNAG 3 cut(s) 693, 726, 981
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 5 cut(s) 103, 219, 432, 657, 994
BstMAI GTCTC 3 cut(s) 89, 245, 962
BstMWI GCNNNNNNNGC 3 cut(s) 499, 518, 783
BstNI CCWGG 2 cut(s) 95, 424
BstSCI CCNGG 4 cut(s) 37, 93, 422, 717
BstSFI CTRYAG 1 cut(s) 741
BstSLI GKGCMC 1 cut(s) 933
BstSNI TACGTA 1 cut(s) 545
BstV1I GCAGC 4 cut(s) 496, 499, 803, 864
BstV2I GAAGAC 1 cut(s) 665
BstXI CCANNNNNNTGG 1 cut(s) 193
Bsu36I CCTNAGG 1 cut(s) 693
BsuI GTATCC 2 cut(s) 394, 916
BsuRI GGCC 5 cut(s) 9, 22, 502, 521, 962
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 5 cut(s) 103, 219, 432, 657, 994
BtsIMutI CAGTG 2 cut(s) 228, 530
BveI ACCTGC 1 cut(s) 742
Cac8I GCNNGC 3 cut(s) 14, 507, 804
Cfr13I GGNCC 4 cut(s) 20, 164, 500, 960
Csp6I GTAC 2 cut(s) 420, 635
CviAII CATG 8 cut(s) 187, 193, 255, 638, 646, 664, 713, 1012
CviQI GTAC 2 cut(s) 420, 635
DdeI CTNAG 3 cut(s) 693, 726, 981
DraI TTTAAA 2 cut(s) 46, 306
EaeI YGGCCR 2 cut(s) 7, 519
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
EciI GGCGGA 1 cut(s) 1001
Eco105I TACGTA 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 779
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 693
EcoRI GAATTC 2 cut(s) 384, 437
EcoRII CCWGG 2 cut(s) 93, 422
EcoT14I CCWWGG 1 cut(s) 167
EcoT22I ATGCAT 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 779
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 8 cut(s) 190, 196, 258, 641, 649, 667, 716, 1015
FaqI GGGAC 3 cut(s) 218, 601, 701
FatI CATG 8 cut(s) 186, 192, 254, 637, 645, 663, 712, 1011
FauNDI CATATG 1 cut(s) 65
FbaI TGATCA 2 cut(s) 106, 346
Fnu4HI GCNGC 7 cut(s) 10, 364, 503, 510, 513, 792, 878
FokI GGATG 5 cut(s) 110, 226, 439, 664, 1001
FriOI GRGCYC 1 cut(s) 779
Fsp4HI GCNGC 7 cut(s) 10, 364, 503, 510, 513, 792, 878
FspBI CTAG 2 cut(s) 17, 807
GluI GCNGC 7 cut(s) 10, 364, 503, 510, 513, 792, 878
GsuI CTGGAG 2 cut(s) 554, 603
HaeIII GGCC 5 cut(s) 9, 22, 502, 521, 962
HapII CCGG 2 cut(s) 39, 719
Hin1II CATG 8 cut(s) 190, 196, 258, 641, 649, 667, 716, 1015
HincII GTYRAC 1 cut(s) 393
HindII GTYRAC 1 cut(s) 393
HinfI GANTC 2 cut(s) 551, 1022
HpaII CCGG 2 cut(s) 39, 719
HphI GGTGA 5 cut(s) 116, 143, 346, 575, 709
Hpy166II GTNNAC 4 cut(s) 164, 393, 566, 635
Hpy188I TCNGA 6 cut(s) 471, 573, 760, 844, 873, 982
Hpy188III TCNNGA 3 cut(s) 88, 577, 620
Hpy8I GTNNAC 4 cut(s) 164, 393, 566, 635
Hpy99I CGWCG 2 cut(s) 411, 414
HpyAV CCTTC 5 cut(s) 574, 590, 846, 994, 1010
HpyCH4III ACNGT 4 cut(s) 131, 149, 161, 301
HpyCH4IV ACGT 2 cut(s) 544, 568
HpyCH4V TGCA 5 cut(s) 515, 667, 751, 850, 880
HpyF10VI GCNNNNNNNGC 3 cut(s) 499, 518, 783
HpyF3I CTNAG 3 cut(s) 693, 726, 981
HpySE526I ACGT 2 cut(s) 544, 568
Hsp92II CATG 8 cut(s) 190, 196, 258, 641, 649, 667, 716, 1015
Ksp22I TGATCA 2 cut(s) 106, 346
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 2 cut(s) 376, 484
Lsp1109I GCAGC 4 cut(s) 496, 499, 803, 864
LweI GCATC 3 cut(s) 623, 864, 889
MaeI CTAG 2 cut(s) 17, 807
MaeII ACGT 2 cut(s) 544, 568
MaeIII GTNAC 1 cut(s) 528
MboII GAAGA 5 cut(s) 278, 670, 831, 928, 931
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 3 cut(s) 154, 779, 933
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 5 cut(s) 312, 384, 437, 450, 845
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 1 cut(s) 545
MmeI TCCRAC 2 cut(s) 606, 701
Mox20I TGGCCA 1 cut(s) 521
Mph1103I ATGCAT 1 cut(s) 669
MscI TGGCCA 1 cut(s) 521
MseI TTAA 2 cut(s) 45, 305
MslI CAYNNNNRTG 4 cut(s) 191, 650, 888, 936
Msp20I TGGCCA 1 cut(s) 521
MspI CCGG 2 cut(s) 39, 719
MspR9I CCNGG 4 cut(s) 39, 95, 424, 719
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 2 cut(s) 95, 424
MwoI GCNNNNNNNGC 3 cut(s) 499, 518, 783
NciI CCSGG 2 cut(s) 39, 719
NdeI CATATG 1 cut(s) 65
NlaIII CATG 8 cut(s) 190, 196, 258, 641, 649, 667, 716, 1015
NlaIV GGNNCC 1 cut(s) 932
NmuCI GTSAC 1 cut(s) 528
NsiI ATGCAT 1 cut(s) 669
NspV TTCGAA 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PfeI GAWTC 1 cut(s) 1022
PflMI CCANNNNNTGG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 7 cut(s) 11, 365, 504, 511, 514, 793, 879
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
Ppu21I YACGTR 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 93, 422
PspGI CCWGG 2 cut(s) 93, 422
PspN4I GGNNCC 1 cut(s) 932
PspPI GGNCC 4 cut(s) 20, 164, 500, 960
RsaI GTAC 2 cut(s) 421, 636
RsaNI GTAC 2 cut(s) 420, 635
RseI CAYNNNNRTG 4 cut(s) 191, 650, 888, 936
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 2 cut(s) 45, 305
SatI GCNGC 7 cut(s) 10, 364, 503, 510, 513, 792, 878
Sau96I GGNCC 4 cut(s) 20, 164, 500, 960
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 4 cut(s) 39, 95, 424, 719
SduI GDGCHC 3 cut(s) 154, 779, 933
SfaNI GCATC 3 cut(s) 623, 864, 889
SfcI CTRYAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 289
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 4 cut(s) 191, 650, 888, 936
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
SnaBI TACGTA 1 cut(s) 545
Sse9I AATT 5 cut(s) 312, 384, 437, 450, 845
SsiI CCGC 7 cut(s) 10, 263, 364, 503, 593, 649, 986
SspMI CTAG 2 cut(s) 17, 807
StyD4I CCNGG 4 cut(s) 37, 93, 422, 717
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 4 cut(s) 131, 149, 161, 301
TaiI ACGT 2 cut(s) 547, 571
TaqI TCGA 3 cut(s) 289, 412, 855
TasI AATT 5 cut(s) 312, 384, 437, 450, 845
TatI WGTACW 1 cut(s) 634
TauI GCSGC 3 cut(s) 12, 366, 505
TfiI GAWTC 1 cut(s) 1022
Tru1I TTAA 2 cut(s) 45, 305
Tru9I TTAA 2 cut(s) 45, 305
TscAI CASTG 2 cut(s) 228, 537
TseFI GTSAC 1 cut(s) 528
TseI GCWGC 4 cut(s) 509, 512, 791, 877
Tsp45I GTSAC 1 cut(s) 528
TspDTI ATGAA 1 cut(s) 492
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 2 cut(s) 228, 537
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 2 cut(s) 384, 437
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XspI CTAG 2 cut(s) 17, 807
Zsp2I ATGCAT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.