Rmu_sc0006223.1_g000038

Protein of unknown function (DUF627)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006223.1
OG0030695
Physical Location & Seq
Reverse (-)
188694 .. 189595
902 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 657 bp
atggggaataagaagcgaaacgctgctccgcgttccaaacagtcgccggcggcggttgcgccgattgtcgatggcggagaggccgtcgttttggcccagcccgattctgctcccctcactctcgccgacgccgagtccaatagccctaacagcaacaaccacaacaacaacctgctagtcccgagcaacaacaagatcgaatcctcgccgtcgatcgaatcgggcggtggtaaggtgtttgattcggaggcttgcgccgccgcgaagctcgaatgcgagagggcgctgacggcgctccggcgcgggaaccacacgaagtgccgccctcgtcgccggactggccgaagtgtgagcagcaggtgcagatcgccgagatcttgtgctgcaggtcggattgggctgcaggcggtctgtgtccggtggaggagagcaggtgaggtctcggtagggagcaggctgcgcaggcgggttgcgggtccggtgctgggttgcagggatccgccttgctgtgcggggatggggccggtcttggcctgtggggtgagactggtgagggagaaacgagggtggaggctgaaggcacggggaaaaaagaagaaaaagttttgttcttctagggttgtgatttttcctcgatgggaagaagaaagaaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF627 DUF629 UCH

Protein Analysis

218

Amino Acids

23.58

Weight (kDa)

10.96

Isoelectric Point (pI)

90.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0030695)

Species Orthologous Gene IDs
rosa_laevigata RLG00000012720
rosa_multiflora Rmu_sc0006223.1_g000038

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 348, 422
Acc16I TGCGCA 1 cut(s) 461
Acc36I ACCTGC 4 cut(s) 180, 348, 377, 422
AccII CGCG 3 cut(s) 31, 263, 303
AclWI GGATC 2 cut(s) 491, 504
AcoI YGGCCR 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 129
AdeI CACNNNGTG 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 485
AhdI GACNNNNNGTC 1 cut(s) 133
AluBI AGCT 1 cut(s) 268
AluI AGCT 1 cut(s) 268
Alw26I GTCTC 2 cut(s) 445, 538
AlwI GGATC 2 cut(s) 491, 504
Ama87I CYCGRG 1 cut(s) 181
AoxI GGCC 5 cut(s) 81, 93, 340, 521, 531
ApeKI GCWGC 5 cut(s) 23, 354, 383, 400, 457
AspLEI GCGC 6 cut(s) 61, 257, 286, 295, 303, 462
AspS9I GGNCC 3 cut(s) 94, 476, 521
AsuHPI GGTGA 3 cut(s) 446, 553, 562
AvaI CYCGRG 1 cut(s) 181
AvaII GGWCC 1 cut(s) 476
BamHI GGATCC 1 cut(s) 496
BbvI GCAGC 5 cut(s) 10, 366, 370, 387, 444
BccI CCATC 3 cut(s) 65, 511, 630
BceAI ACGGC 3 cut(s) 68, 193, 306
BcoDI GTCTC 2 cut(s) 445, 538
BfaI CTAG 3 cut(s) 176, 615, 655
BfmI CTRYAG 2 cut(s) 384, 401
BfoI RGCGCY 2 cut(s) 287, 296
BfuAI ACCTGC 4 cut(s) 180, 348, 377, 422
BglI GCCNNNNNGGC 1 cut(s) 339
BglII AGATCT 1 cut(s) 374
BisI GCNGC 9 cut(s) 24, 51, 258, 261, 322, 355, 384, 401, 458
BlsI GCNGC 9 cut(s) 25, 52, 259, 262, 323, 356, 385, 402, 459
Bme18I GGWCC 1 cut(s) 476
BmeRI GACNNNNNGTC 1 cut(s) 133
BmeT110I CYCGRG 1 cut(s) 181
BmgT120I GGNCC 3 cut(s) 94, 476, 521
BmiI GGNNCC 4 cut(s) 308, 477, 498, 522
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 445
BsaWI WCCGGW 2 cut(s) 417, 478
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 418, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse118I RCCGGY 2 cut(s) 46, 523
Bse1I ACTGG 2 cut(s) 343, 552
BseGI GGATG 1 cut(s) 522
BseLI CCNNNNNNNGG 1 cut(s) 485
BseNI ACTGG 2 cut(s) 343, 552
BseRI GAGGAG 1 cut(s) 439
BseXI GCAGC 5 cut(s) 10, 366, 370, 387, 444
BseYI CCCAGC 2 cut(s) 96, 484
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 3 cut(s) 31, 263, 303
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 5 cut(s) 83, 95, 342, 523, 533
BsiEI CGRYCG 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 181
BsiSI CCGG 6 cut(s) 47, 298, 334, 418, 479, 524
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 2 cut(s) 445, 538
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 5 cut(s) 83, 95, 342, 523, 533
Bso31I GGTCTC 1 cut(s) 445
BsoBI CYCGRG 1 cut(s) 181
Bsp143I GATC 5 cut(s) 195, 213, 365, 374, 496
BspANI GGCC 5 cut(s) 83, 95, 342, 523, 533
BspFNI CGCG 3 cut(s) 31, 263, 303
BspLI GGNNCC 4 cut(s) 308, 477, 498, 522
BspMAI CTGCAG 2 cut(s) 388, 405
BspMI ACCTGC 4 cut(s) 180, 348, 377, 422
BspPI GGATC 2 cut(s) 491, 504
BspTNI GGTCTC 1 cut(s) 445
BsrFI RCCGGY 2 cut(s) 46, 523
BsrI ACTGG 2 cut(s) 343, 552
BssAI RCCGGY 2 cut(s) 46, 523
BssMI GATC 5 cut(s) 195, 213, 365, 374, 496
BssNI GRCGYC 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 42
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 360
BstC8I GCNNGC 5 cut(s) 48, 253, 405, 455, 464
BstF5I GGATG 1 cut(s) 522
BstFNI CGCG 3 cut(s) 31, 263, 303
BstH2I RGCGCY 2 cut(s) 287, 296
BstHHI GCGC 6 cut(s) 61, 257, 286, 295, 303, 462
BstKTI GATC 5 cut(s) 198, 216, 368, 377, 499
BstMAI GTCTC 2 cut(s) 445, 538
BstMBI GATC 5 cut(s) 195, 213, 365, 374, 496
BstMCI CGRYCG 1 cut(s) 216
BstSFI CTRYAG 2 cut(s) 384, 401
BstUI CGCG 3 cut(s) 31, 263, 303
BstV1I GCAGC 5 cut(s) 10, 366, 370, 387, 444
BstX2I RGATCY 2 cut(s) 374, 496
BstYI RGATCY 2 cut(s) 374, 496
BsuRI GGCC 5 cut(s) 83, 95, 342, 523, 533
BtsCI GGATG 1 cut(s) 522
BveI ACCTGC 4 cut(s) 180, 348, 377, 422
Cac8I GCNNGC 5 cut(s) 48, 253, 405, 455, 464
CfoI GCGC 6 cut(s) 61, 257, 286, 295, 303, 462
Cfr10I RCCGGY 2 cut(s) 46, 523
Cfr13I GGNCC 3 cut(s) 94, 476, 521
CseI GACGC 1 cut(s) 137
DpnI GATC 5 cut(s) 197, 215, 367, 376, 498
DpnII GATC 5 cut(s) 195, 213, 365, 374, 496
DraIII CACNNNGTG 1 cut(s) 318
DriI GACNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 340
Eam1105I GACNNNNNGTC 1 cut(s) 133
EciI GGCGGA 2 cut(s) 90, 489
Eco31I GGTCTC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 181
FaqI GGGAC 1 cut(s) 164
FauI CCCGC 4 cut(s) 296, 459, 466, 505
Fnu4HI GCNGC 9 cut(s) 24, 51, 258, 261, 322, 355, 384, 401, 458
FokI GGATG 1 cut(s) 529
Fsp4HI GCNGC 9 cut(s) 24, 51, 258, 261, 322, 355, 384, 401, 458
FspBI CTAG 3 cut(s) 176, 615, 655
FspI TGCGCA 1 cut(s) 461
GlaI GCGC 6 cut(s) 60, 256, 285, 294, 302, 461
GluI GCNGC 9 cut(s) 24, 51, 258, 261, 322, 355, 384, 401, 458
GsaI CCCAGC 2 cut(s) 100, 488
HaeII RGCGCY 2 cut(s) 287, 296
HaeIII GGCC 5 cut(s) 83, 95, 342, 523, 533
HapII CCGG 6 cut(s) 47, 298, 334, 418, 479, 524
HgaI GACGC 1 cut(s) 137
HhaI GCGC 6 cut(s) 61, 257, 286, 295, 303, 462
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 6 cut(s) 59, 255, 284, 293, 301, 460
HinP1I GCGC 6 cut(s) 59, 255, 284, 293, 301, 460
HinfI GANTC 5 cut(s) 104, 134, 200, 218, 242
HpaII CCGG 6 cut(s) 47, 298, 334, 418, 479, 524
HphI GGTGA 3 cut(s) 446, 553, 562
Hpy188I TCNGA 2 cut(s) 247, 393
Hpy188III TCNNGA 1 cut(s) 181
Hpy99I CGWCG 4 cut(s) 89, 131, 214, 333
HpyAV CCTTC 1 cut(s) 571
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4V TGCA 4 cut(s) 363, 386, 403, 492
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 6 cut(s) 59, 255, 284, 293, 301, 460
KroI GCCGGC 1 cut(s) 46
KroNI GCCGGC 1 cut(s) 48
Kzo9I GATC 5 cut(s) 195, 213, 365, 374, 496
LmnI GCTCC 4 cut(s) 31, 115, 300, 450
Lsp1109I GCAGC 5 cut(s) 10, 366, 370, 387, 444
MaeI CTAG 3 cut(s) 176, 615, 655
MalI GATC 5 cut(s) 197, 215, 367, 376, 498
MboI GATC 5 cut(s) 195, 213, 365, 374, 496
MboII GAAGA 4 cut(s) 603, 607, 653, 656
MflI RGATCY 2 cut(s) 374, 496
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 371
MreI CGCCGGCG 1 cut(s) 46
MroNI GCCGGC 1 cut(s) 46
MspI CCGG 6 cut(s) 47, 298, 334, 418, 479, 524
Mva1269I GAATGC 1 cut(s) 278
MvnI CGCG 3 cut(s) 31, 263, 303
NaeI GCCGGC 1 cut(s) 48
NdeII GATC 5 cut(s) 195, 213, 365, 374, 496
NgoMIV GCCGGC 1 cut(s) 46
NlaIV GGNNCC 4 cut(s) 308, 477, 498, 522
NmeAIII GCCGAG 2 cut(s) 157, 396
NsbI TGCGCA 1 cut(s) 461
PaqCI CACCTGC 2 cut(s) 348, 422
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 1 cut(s) 278
PdiI GCCGGC 1 cut(s) 48
PfeI GAWTC 4 cut(s) 104, 200, 218, 242
PkrI GCNGC 9 cut(s) 25, 52, 259, 262, 323, 356, 385, 402, 459
Ple19I CGATCG 1 cut(s) 216
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PspFI CCCAGC 2 cut(s) 96, 484
PspN4I GGNNCC 4 cut(s) 308, 477, 498, 522
PspPI GGNCC 3 cut(s) 94, 476, 521
PstI CTGCAG 2 cut(s) 388, 405
PsuI RGATCY 2 cut(s) 374, 496
PvuI CGATCG 1 cut(s) 216
SatI GCNGC 9 cut(s) 24, 51, 258, 261, 322, 355, 384, 401, 458
Sau3AI GATC 5 cut(s) 195, 213, 365, 374, 496
Sau96I GGNCC 3 cut(s) 94, 476, 521
SchI GAGTC 1 cut(s) 143
SetI ASST 7 cut(s) 174, 237, 270, 362, 391, 436, 441
SfcI CTRYAG 2 cut(s) 384, 401
SgrAI CRCCGGYG 1 cut(s) 46
SinI GGWCC 1 cut(s) 476
SspMI CTAG 3 cut(s) 176, 615, 655
TaaI ACNGT 1 cut(s) 42
TaqI TCGA 6 cut(s) 69, 198, 212, 216, 270, 634
TauI GCSGC 4 cut(s) 53, 260, 263, 324
TfiI GAWTC 4 cut(s) 104, 200, 218, 242
TseI GCWGC 5 cut(s) 23, 354, 383, 400, 457
VpaK11BI GGWCC 1 cut(s) 476
XspI CTAG 3 cut(s) 176, 615, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.