Rmu_sc0006499.1_g000010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006499.1
OG0023021
Physical Location & Seq
Forward (+)
36316 .. 41909
5594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006499.1_g000010.1.cds

Sequence Viewer

Length: 786 bp
atgccagcggcaataccgacaccgctagggagcacaacgctaacggccacagtcaacaccgtccacaagtgcagcagtgttggcgtcacatcaactccaatttggcgaagttatgtgcctaaaggcttcatattcgtcgtggcggaccaccagcaggaacctcgtctcgctgttgcctaccctcttgtgacctctgatcgcccatgcatcgatcgtagacggagaatcaacaatccaaagtttgaagaacctctggcgagtttcaacgaatttcggcgactctggccaccttttgcgatgtatttgccttttctgcctccacctcacaccttgaagaacttgatcccaggagtccagaaccggcagccccaccttaagaaccggccggaaaaccaccgaaccggccaccggaatctctatacaactagcagcaaatattccaccggttcaccatcttccggacctccaatttccaccaaaatatggatgagtcgcctgaacaaattggactttgctccattgggaactactggctctgaatatcacaggtgggtttgtgatatccgccagcatctcaaggccgatggaatcttggatacgattctcgagcctagccaggacgtgctaactgttgagcaagctcaggctttggaagcaaatagagcggccttagaggcaaataacgcgaaagccatcatcctaatgactcgtcatatggatgattcgctccaatatgagtgtataaatgaagaagaccccagaaggctatgggtctcactccaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

261

Amino Acids

29.56

Weight (kDa)

9.23

Isoelectric Point (pI)

61.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023021)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001467.1_g000018 Rmu_sc0002157.1_g000007 Rmu_sc0006499.1_g000010 Rmu_ssc0000151.1_g000003

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 483
AccBSI CCGCTC 1 cut(s) 665
AccI GTMKAC 1 cut(s) 217
AccII CGCG 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 458
AciI CCGC 5 cut(s) 8, 23, 143, 565, 665
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 4 cut(s) 45, 284, 383, 403
AcsI RAATTY 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 84
AfiI CCNNNNNNNGG 4 cut(s) 154, 408, 458, 483
AflII CTTAAG 1 cut(s) 374
AgeI ACCGGT 1 cut(s) 443
AgsI TTSAA 3 cut(s) 245, 265, 334
AjiI CACGTC 1 cut(s) 622
AjnI CCWGG 2 cut(s) 346, 615
AluBI AGCT 1 cut(s) 641
AluI AGCT 1 cut(s) 641
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 2 cut(s) 170, 778
AlwI GGATC 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 605
Aor13HI TCCGGA 1 cut(s) 458
AoxI GGCC 6 cut(s) 45, 284, 383, 403, 579, 666
ApeKI GCWGC 3 cut(s) 72, 364, 429
ApoI RAATTY 1 cut(s) 269
AsiGI ACCGGT 1 cut(s) 443
AspS9I GGNCC 2 cut(s) 145, 461
AsuHPI GGTGA 1 cut(s) 441
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 2 cut(s) 145, 461
BalI TGGCCA 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 759
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 84, 376, 441
BccI CCATC 3 cut(s) 460, 578, 701
BceAI ACGGC 1 cut(s) 60
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 286, 320
BciT130I CCWGG 2 cut(s) 348, 617
BciVI GTATCC 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 170, 778
BfaI CTAG 3 cut(s) 26, 426, 612
BfrI CTTAAG 1 cut(s) 374
BfuI GTATCC 1 cut(s) 589
BglI GCCNNNNNGGC 1 cut(s) 674
BisI GCNGC 5 cut(s) 9, 73, 365, 430, 666
BlsI GCNGC 5 cut(s) 10, 74, 366, 431, 667
Bme1390I CCNGG 2 cut(s) 348, 617
Bme18I GGWCC 2 cut(s) 145, 461
BmeT110I CYCGRG 1 cut(s) 605
BmgBI CACGTC 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 145, 461
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 348, 617
BmsI GCATC 2 cut(s) 216, 580
BpiI GAAGAC 1 cut(s) 759
Bpu10I CCTNAGC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 560
Bsa29I ATCGAT 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 778
BsaJI CCNNGG 1 cut(s) 346
BsaWI WCCGGW 3 cut(s) 408, 443, 458
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 408, 458, 483
Bse118I RCCGGY 4 cut(s) 360, 381, 401, 443
Bse1I ACTGG 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 458
BseBI CCWGG 2 cut(s) 348, 617
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 3 cut(s) 492, 696, 724
BseLI CCNNNNNNNGG 4 cut(s) 154, 408, 458, 483
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 1 cut(s) 535
BseX3I CGGCCG 1 cut(s) 383
BseXI GCAGC 3 cut(s) 84, 376, 441
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 1 cut(s) 686
Bsh1285I CGRYCG 2 cut(s) 214, 386
BshFI GGCC 6 cut(s) 47, 286, 385, 405, 581, 668
BshTI ACCGGT 1 cut(s) 443
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 2 cut(s) 214, 386
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 7 cut(s) 361, 382, 386, 402, 409, 444, 459
BslI CCNNNNNNNGG 4 cut(s) 154, 408, 458, 483
BsmAI GTCTC 2 cut(s) 170, 778
BsmBI CGTCTC 1 cut(s) 170
BsnI GGCC 6 cut(s) 47, 286, 385, 405, 581, 668
Bso31I GGTCTC 1 cut(s) 778
BsoBI CYCGRG 1 cut(s) 605
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 1 cut(s) 458
Bsp143I GATC 3 cut(s) 196, 211, 342
BspACI CCGC 5 cut(s) 8, 23, 143, 565, 665
BspANI GGCC 6 cut(s) 47, 286, 385, 405, 581, 668
BspCNI CTCAG 1 cut(s) 655
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 1 cut(s) 458
BspFNI CGCG 1 cut(s) 686
BspLI GGNNCC 1 cut(s) 159
BspPI GGATC 1 cut(s) 337
BspTI CTTAAG 1 cut(s) 374
BspTNI GGTCTC 1 cut(s) 778
BsrBI CCGCTC 1 cut(s) 665
BsrFI RCCGGY 4 cut(s) 360, 381, 401, 443
BsrI ACTGG 1 cut(s) 535
BssAI RCCGGY 4 cut(s) 360, 381, 401, 443
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 3 cut(s) 196, 211, 342
BssNI GRCGYC 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 348, 617
Bst4CI ACNGT 3 cut(s) 52, 61, 631
BstACI GRCGYC 1 cut(s) 84
BstAFI CTTAAG 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 6, 569, 639
BstDEI CTNAG 2 cut(s) 642, 670
BstF5I GGATG 3 cut(s) 492, 696, 724
BstFNI CGCG 1 cut(s) 686
BstKTI GATC 3 cut(s) 199, 214, 345
BstMAI GTCTC 2 cut(s) 170, 778
BstMBI GATC 3 cut(s) 196, 211, 342
BstMCI CGRYCG 2 cut(s) 214, 386
BstMWI GCNNNNNNNGC 7 cut(s) 81, 283, 313, 653, 662, 674, 683
BstNI CCWGG 2 cut(s) 348, 617
BstSCI CCNGG 2 cut(s) 346, 615
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 3 cut(s) 84, 376, 441
BstV2I GAAGAC 1 cut(s) 759
BstZI CGGCCG 1 cut(s) 383
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 6 cut(s) 47, 286, 385, 405, 581, 668
BsuTUI ATCGAT 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 311
BtrI CACGTC 1 cut(s) 622
BtsCI GGATG 3 cut(s) 492, 696, 724
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 3 cut(s) 6, 569, 639
Cfr10I RCCGGY 4 cut(s) 360, 381, 401, 443
Cfr13I GGNCC 2 cut(s) 145, 461
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 1 cut(s) 73
CspAI ACCGGT 1 cut(s) 443
CviAII CATG 1 cut(s) 204
DdeI CTNAG 2 cut(s) 642, 670
DpnI GATC 3 cut(s) 198, 213, 344
DpnII GATC 3 cut(s) 196, 211, 342
EaeI YGGCCR 4 cut(s) 45, 284, 383, 403
EagI CGGCCG 1 cut(s) 383
EciI GGCGGA 2 cut(s) 158, 554
EclXI CGGCCG 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 778
Eco32I GATATC 1 cut(s) 562
Eco47I GGWCC 2 cut(s) 145, 461
Eco52I CGGCCG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 605
EcoRII CCWGG 2 cut(s) 346, 615
EcoRV GATATC 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 209
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 1 cut(s) 207
FatI CATG 1 cut(s) 203
FauNDI CATATG 1 cut(s) 714
FblI GTMKAC 1 cut(s) 217
Fnu4HI GCNGC 5 cut(s) 9, 73, 365, 430, 666
FokI GGATG 3 cut(s) 499, 683, 731
Fsp4HI GCNGC 5 cut(s) 9, 73, 365, 430, 666
FspBI CTAG 3 cut(s) 26, 426, 612
GluI GCNGC 5 cut(s) 9, 73, 365, 430, 666
HaeIII GGCC 6 cut(s) 47, 286, 385, 405, 581, 668
HapII CCGG 7 cut(s) 361, 382, 386, 402, 409, 444, 459
HgaI GACGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 1 cut(s) 207
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 9 cut(s) 225, 279, 351, 412, 490, 588, 601, 706, 722
HpaII CCGG 7 cut(s) 361, 382, 386, 402, 409, 444, 459
HphI GGTGA 1 cut(s) 441
Hpy166II GTNNAC 4 cut(s) 55, 64, 218, 449
Hpy188I TCNGA 2 cut(s) 196, 538
Hpy188III TCNNGA 3 cut(s) 355, 459, 605
Hpy8I GTNNAC 4 cut(s) 55, 64, 218, 449
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 756
HpyCH4III ACNGT 3 cut(s) 52, 61, 631
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 2 cut(s) 72, 207
HpyF10VI GCNNNNNNNGC 7 cut(s) 81, 283, 313, 653, 662, 674, 683
HpyF3I CTNAG 2 cut(s) 642, 670
HpySE526I ACGT 1 cut(s) 621
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 1 cut(s) 207
Kpn2I TCCGGA 1 cut(s) 458
Kzo9I GATC 3 cut(s) 196, 211, 342
LmnI GCTCC 3 cut(s) 30, 520, 732
Lsp1109I GCAGC 3 cut(s) 84, 376, 441
LweI GCATC 2 cut(s) 216, 580
MaeI CTAG 3 cut(s) 26, 426, 612
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 2 cut(s) 85, 187
MalI GATC 3 cut(s) 198, 213, 344
MbiI CCGCTC 1 cut(s) 665
MboI GATC 3 cut(s) 196, 211, 342
MboII GAAGA 5 cut(s) 257, 346, 447, 761, 764
MhlI GDGCHC 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 4 cut(s) 99, 269, 468, 503
MluNI TGGCCA 1 cut(s) 286
MlyI GAGTC 4 cut(s) 273, 360, 499, 700
MnlI CCTC 8 cut(s) 171, 192, 202, 261, 327, 333, 474, 667
Mox20I TGGCCA 1 cut(s) 286
Mph1103I ATGCAT 1 cut(s) 209
MroI TCCGGA 1 cut(s) 458
MscI TGGCCA 1 cut(s) 286
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 2 cut(s) 701, 717
Msp20I TGGCCA 1 cut(s) 286
MspA1I CMGCKG 1 cut(s) 8
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 7 cut(s) 361, 382, 386, 402, 409, 444, 459
MspR9I CCNGG 2 cut(s) 348, 617
MvaI CCWGG 2 cut(s) 348, 617
MvnI CGCG 1 cut(s) 686
MwoI GCNNNNNNNGC 7 cut(s) 81, 283, 313, 653, 662, 674, 683
NdeI CATATG 1 cut(s) 714
NdeII GATC 3 cut(s) 196, 211, 342
NlaIII CATG 1 cut(s) 207
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 2 cut(s) 85, 187
NsiI ATGCAT 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 605
PfeI GAWTC 5 cut(s) 225, 412, 588, 601, 722
PflMI CCANNNNNTGG 1 cut(s) 483
PinAI ACCGGT 1 cut(s) 443
PkrI GCNGC 5 cut(s) 10, 74, 366, 431, 667
Ple19I CGATCG 1 cut(s) 214
PleI GAGTC 4 cut(s) 273, 359, 498, 700
PpsI GAGTC 4 cut(s) 273, 359, 498, 700
Psp6I CCWGG 2 cut(s) 346, 615
PspGI CCWGG 2 cut(s) 346, 615
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 2 cut(s) 145, 461
PvuI CGATCG 1 cut(s) 214
RseI CAYNNNNRTG 2 cut(s) 701, 717
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 5 cut(s) 9, 73, 365, 430, 666
Sau3AI GATC 3 cut(s) 196, 211, 342
Sau96I GGNCC 2 cut(s) 145, 461
SchI GAGTC 4 cut(s) 273, 360, 499, 700
ScrFI CCNGG 2 cut(s) 348, 617
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 2 cut(s) 216, 580
Sfr274I CTCGAG 1 cut(s) 605
SinI GGWCC 2 cut(s) 145, 461
SlaI CTCGAG 1 cut(s) 605
SmiMI CAYNNNNRTG 2 cut(s) 701, 717
SmlI CTYRAG 3 cut(s) 374, 575, 605
SmoI CTYRAG 3 cut(s) 374, 575, 605
Sse9I AATT 4 cut(s) 99, 269, 468, 503
SsiI CCGC 5 cut(s) 8, 23, 143, 565, 665
SspI AATATT 1 cut(s) 437
SspMI CTAG 3 cut(s) 26, 426, 612
StyD4I CCNGG 2 cut(s) 346, 615
TaaI ACNGT 3 cut(s) 52, 61, 631
TaiI ACGT 1 cut(s) 624
TaqI TCGA 2 cut(s) 210, 606
TasI AATT 4 cut(s) 99, 269, 468, 503
TauI GCSGC 2 cut(s) 11, 668
TfiI GAWTC 5 cut(s) 225, 412, 588, 601, 722
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 2 cut(s) 85, 187
TseI GCWGC 3 cut(s) 72, 364, 429
Tsp45I GTSAC 2 cut(s) 85, 187
TspDTI ATGAA 2 cut(s) 118, 762
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 1 cut(s) 82
Van91I CCANNNNNTGG 1 cut(s) 483
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 145, 461
XapI RAATTY 1 cut(s) 269
XhoI CTCGAG 1 cut(s) 605
XmiI GTMKAC 1 cut(s) 217
XspI CTAG 3 cut(s) 26, 426, 612
Zsp2I ATGCAT 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.