Rmu_sc0007459.1_g000002

MACPF domain-containing protein CAD1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007459.1
OG0028052
Physical Location & Seq
Forward (+)
2794 .. 5564
2771 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 789 bp
atgggggaaaatgtagcagctttgcacactgctgttaactctgtacaggcactgggaagagggtttgacgtgaactttgatacgaggctgctttattgtaagggtgtggcagggtctagggtactagagcttgatgaggagcacaccagggacctctgcctttatgatgatgtagttgtgcccaatgtttccaaggatattaagcgctgtcaggagtccagtgggcggcggagttctggagtctgcagcttcaatgagatggttgagtattttaatcgaaagtctaatatatctggaggctatcctgtggggagtttcaattttgcatttagcttcagcggttcaaagcatgttgatatggcaggaacaaagacactttctatggatggcctctatgttccacttgctaagatccatcttttaaaatccccactaatgttacaacaaaatgtgaaacaggctgtcccaacttgttgggatcccaaatccttggcaagctttattgaaaattttggtactcatgtcataacatctgtaactattggtggcaaagatgtaatttacgttaaacaacaccagtcttctcctttgtcaaccatggagatcaagaactacatccaagacattggaaatcagaggttctcaaacacagaaagcaatacaagttcagttcaaatgaatttcaaggataaggggtctgattccggtttattcaacagtcaggggatataccctcaacccaccactgcaccacatcttgctgggaaagaagtatgtaacaactactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

262

Amino Acids

28.8

Weight (kDa)

7.03

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 104 - 234 7.9e-18 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028052)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g10020
rosa_multiflora Rmu_sc0007459.1_g000002

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 226, 229, 339
AclWI GGATC 3 cut(s) 406, 473, 486
AcsI RAATTY 2 cut(s) 508, 679
AcuI CTGAAG 1 cut(s) 319
AfaI GTAC 3 cut(s) 45, 123, 517
AfeI AGCGCT 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 225
AgsI TTSAA 7 cut(s) 253, 319, 345, 506, 674, 685, 715
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 5 cut(s) 20, 130, 249, 333, 498
AluI AGCT 5 cut(s) 20, 130, 249, 333, 498
Alw21I GWGCWC 1 cut(s) 144
AlwI GGATC 3 cut(s) 406, 473, 486
AlwNI CAGNNNCTG 1 cut(s) 52
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 1 cut(s) 388
ApeKI GCWGC 3 cut(s) 17, 88, 246
ApoI RAATTY 2 cut(s) 508, 679
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 151
AvaII GGWCC 1 cut(s) 151
BaeGI GKGCMC 1 cut(s) 183
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BamHI GGATCC 1 cut(s) 478
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 29, 75, 258
BccI CCATC 3 cut(s) 253, 380, 423
BciT130I CCWGG 1 cut(s) 148
BfaI CTAG 2 cut(s) 117, 125
BfmI CTRYAG 1 cut(s) 244
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 4 cut(s) 18, 89, 227, 247
BlsI GCNGC 4 cut(s) 19, 90, 228, 248
Bme1390I CCNGG 1 cut(s) 148
Bme18I GGWCC 1 cut(s) 151
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 152, 480
BmrFI CCNGG 1 cut(s) 148
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 573
BpmI CTGGAG 2 cut(s) 258, 315
BsaJI CCNNGG 4 cut(s) 147, 192, 489, 597
BsaWI WCCGGW 1 cut(s) 704
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 3 cut(s) 57, 219, 577
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 4 cut(s) 147, 192, 489, 597
BseGI GGATG 2 cut(s) 391, 615
BseLI CCNNNNNNNGG 1 cut(s) 225
BseNI ACTGG 3 cut(s) 57, 219, 577
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 3 cut(s) 29, 75, 258
BseYI CCCAGC 1 cut(s) 761
BsgI GTGCAG 1 cut(s) 732
BshFI GGCC 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 705
BslFI GGGAC 2 cut(s) 164, 449
BslI CCNNNNNNNGG 1 cut(s) 225
BsmFI GGGAC 2 cut(s) 164, 449
BsnI GGCC 1 cut(s) 390
Bsp1286I GDGCHC 2 cut(s) 144, 183
Bsp1407I TGTACA 1 cut(s) 43
Bsp143I GATC 3 cut(s) 411, 478, 603
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 3 cut(s) 226, 229, 339
BspANI GGCC 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 152, 480
BspMAI CTGCAG 1 cut(s) 248
BspPI GGATC 3 cut(s) 406, 473, 486
BsrGI TGTACA 1 cut(s) 43
BsrI ACTGG 3 cut(s) 57, 219, 577
BssECI CCNNGG 4 cut(s) 147, 192, 489, 597
BssMI GATC 3 cut(s) 411, 478, 603
BssT1I CCWWGG 3 cut(s) 192, 489, 597
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 1 cut(s) 719
Bst6I CTCTTC 1 cut(s) 52
BstAUI TGTACA 1 cut(s) 43
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 2 cut(s) 391, 615
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 414, 481, 606
BstMBI GATC 3 cut(s) 411, 478, 603
BstNI CCWGG 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 146
BstSFI CTRYAG 1 cut(s) 244
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 29, 75, 258
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 2 cut(s) 411, 478
BstXI CCANNNNNNTGG 3 cut(s) 474, 490, 626
BstYI RGATCY 2 cut(s) 411, 478
BsuRI GGCC 1 cut(s) 390
BtgI CCRYGG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 2 cut(s) 391, 615
BtsI GCAGTG 2 cut(s) 27, 744
BtsIMutI CAGTG 4 cut(s) 27, 50, 226, 744
Cac8I GCNNGC 1 cut(s) 496
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 151
Csp6I GTAC 3 cut(s) 44, 122, 516
CviAII CATG 3 cut(s) 350, 521, 598
CviJI RGCY 9 cut(s) 20, 88, 130, 249, 300, 333, 390, 461, 498
CviKI_1 RGCY 9 cut(s) 20, 88, 130, 249, 300, 333, 390, 461, 498
CviQI GTAC 3 cut(s) 44, 122, 516
DdeI CTNAG 1 cut(s) 408
DpnI GATC 3 cut(s) 413, 480, 605
DpnII GATC 3 cut(s) 411, 478, 603
DraI TTTAAA 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 244
Eco130I CCWWGG 3 cut(s) 192, 489, 597
Eco47I GGWCC 1 cut(s) 151
Eco47III AGCGCT 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 3 cut(s) 192, 489, 597
ErhI CCWWGG 3 cut(s) 192, 489, 597
FaeI CATG 3 cut(s) 353, 524, 601
FaqI GGGAC 2 cut(s) 164, 449
FatI CATG 3 cut(s) 349, 520, 597
Fnu4HI GCNGC 4 cut(s) 18, 89, 227, 247
FokI GGATG 2 cut(s) 398, 602
Fsp4HI GCNGC 4 cut(s) 18, 89, 227, 247
FspBI CTAG 2 cut(s) 117, 125
GlaI GCGC 1 cut(s) 206
GluI GCNGC 4 cut(s) 18, 89, 227, 247
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 2 cut(s) 258, 315
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 1 cut(s) 390
HapII CCGG 1 cut(s) 705
HhaI GCGC 1 cut(s) 207
Hin1II CATG 3 cut(s) 353, 524, 601
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 2 cut(s) 37, 594
HindII GTYRAC 2 cut(s) 37, 594
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 3 cut(s) 215, 240, 701
HpaI GTTAAC 1 cut(s) 37
HpaII CCGG 1 cut(s) 705
Hpy166II GTNNAC 3 cut(s) 37, 73, 594
Hpy188I TCNGA 2 cut(s) 636, 700
Hpy188III TCNNGA 4 cut(s) 212, 237, 294, 607
Hpy8I GTNNAC 3 cut(s) 37, 73, 594
HpyCH4III ACNGT 1 cut(s) 719
HpyCH4IV ACGT 2 cut(s) 69, 564
HpyCH4V TGCA 4 cut(s) 25, 246, 326, 749
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 2 cut(s) 69, 564
Hsp92II CATG 3 cut(s) 353, 524, 601
HspAI GCGC 1 cut(s) 205
KspAI GTTAAC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 411, 478, 603
LmnI GCTCC 1 cut(s) 139
Lsp1109I GCAGC 3 cut(s) 29, 75, 258
MaeI CTAG 2 cut(s) 117, 125
MaeII ACGT 2 cut(s) 69, 564
MaeIII GTNAC 3 cut(s) 438, 535, 776
MalI GATC 3 cut(s) 413, 480, 605
MboI GATC 3 cut(s) 411, 478, 603
MboII GAAGA 2 cut(s) 69, 573
MflI RGATCY 2 cut(s) 411, 478
MhlI GDGCHC 2 cut(s) 144, 183
MluCI AATT 4 cut(s) 319, 508, 558, 679
MlyI GAGTC 2 cut(s) 224, 249
MnlI CCTC 8 cut(s) 53, 78, 130, 164, 290, 401, 630, 744
MseI TTAA 5 cut(s) 36, 201, 273, 422, 567
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 1 cut(s) 705
MspR9I CCNGG 1 cut(s) 148
MvaI CCWGG 1 cut(s) 148
NcoI CCATGG 1 cut(s) 597
NdeII GATC 3 cut(s) 411, 478, 603
NlaIII CATG 3 cut(s) 353, 524, 601
NlaIV GGNNCC 2 cut(s) 152, 480
NspI RCATGY 1 cut(s) 353
PfeI GAWTC 1 cut(s) 701
PkrI GCNGC 4 cut(s) 19, 90, 228, 248
PleI GAGTC 2 cut(s) 223, 248
PpsI GAGTC 2 cut(s) 223, 248
PpuMI RGGWCCY 1 cut(s) 151
Psp5II RGGWCCY 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 2 cut(s) 152, 480
PspPI GGNCC 1 cut(s) 151
PspPPI RGGWCCY 1 cut(s) 151
PstI CTGCAG 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 2 cut(s) 411, 478
RsaI GTAC 3 cut(s) 45, 123, 517
RsaNI GTAC 3 cut(s) 44, 122, 516
SaqAI TTAA 5 cut(s) 36, 201, 273, 422, 567
SatI GCNGC 4 cut(s) 18, 89, 227, 247
Sau3AI GATC 3 cut(s) 411, 478, 603
Sau96I GGNCC 1 cut(s) 151
SchI GAGTC 2 cut(s) 224, 249
ScrFI CCNGG 1 cut(s) 148
SduI GDGCHC 2 cut(s) 144, 183
SetI ASST 9 cut(s) 22, 72, 132, 156, 251, 335, 500, 567, 641
SfcI CTRYAG 1 cut(s) 244
SinI GGWCC 1 cut(s) 151
Sse9I AATT 4 cut(s) 319, 508, 558, 679
SsiI CCGC 3 cut(s) 226, 229, 339
SspMI CTAG 2 cut(s) 117, 125
StyD4I CCNGG 1 cut(s) 146
StyI CCWWGG 3 cut(s) 192, 489, 597
TaaI ACNGT 1 cut(s) 719
TaiI ACGT 2 cut(s) 72, 567
TaqI TCGA 1 cut(s) 277
TasI AATT 4 cut(s) 319, 508, 558, 679
TatI WGTACW 1 cut(s) 43
TauI GCSGC 1 cut(s) 229
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 5 cut(s) 36, 201, 273, 422, 567
Tru9I TTAA 5 cut(s) 36, 201, 273, 422, 567
TscAI CASTG 4 cut(s) 34, 57, 226, 751
TseI GCWGC 3 cut(s) 17, 88, 246
TspDTI ATGAA 1 cut(s) 692
TspRI CASTG 4 cut(s) 34, 57, 226, 751
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 2 cut(s) 508, 679
XceI RCATGY 1 cut(s) 353
XspI CTAG 2 cut(s) 117, 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.