FvH4_6g10020

MACPF domain-containing protein CAD1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003665
Physical Location & Seq
Reverse (-)
5943195 .. 5949453
6259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10020.t4

Sequence Viewer

Length: 1773 bp
ATGGGGGAAAATGTAGCAGCTTTGCATACTGCTATTAACGCTGTACAGGCACTGGGAAGAGGGTTTGACGTGAACTTTGATACGAGGCTGCTTTATTGTAAGGGTGTGGCAGGGTCTAGGGTGATAGAGGTTGATGAGGAGCACACCAGGAACCTCTGCCTTTATGATGATGTAGTTGTGCCTAATGTTTCCAGGGATATTAAGCACTCTCACCAGTCCAGTGAGCGGCGGAGTACTGGAGTCTGCAGTTTTAATGAGATGGTCGAGTATTTTAATGATAGGTCTAATATATCTGGAAGCTATCCTGTGGGGAGTTTCAATTTTGCATTTAGCTTCAGTGGTTCAAAGCATGTTGATATGGCAGGAACAAAGACACTTTCTATGGATGGCCTCTATATTCCACTTGCTAAAGTCCATCTTTTAAAATCCCCACTAATGTTACAAGAAAATGTGAAACAGGCTATCCCAACTTGTTGGGATCCCACATCCTTGGCAAGCTTTATTGAAAATTTTGGTACTCATGTCATAACATCTGTAACTATTGGCGGCAAAGATGTAATTTACGTTAAACAACACCAGTCTTCTCCTTTGTCAACCATGGAGATCAAGAACTACATCCAAAACATTGGGAATCAGAGGTTCTCAGGCACAGAAAGCAATACAAGTTCAGGTCAAATGAATTTCAAGGATAAGGGGTCTGATTCCGGTTTATTCAACAGTCAGGGGATCTACCCTCAACCCACTACGGCACCATATCTTGCTGTGAAAGAAGATGTGACTGTCATTTTTCGAAGAAGGGGAGGAGACGAGCTGGAACAAAACCACACCCGGTGGGCAAGAACTGTCCGGTCCTCTCCAGATGTAATTGAGATGACATTTTTTCCTATAGCAGCTCTCCTTGAAGAGGTGATTGGAAGGGAGCACCTTACTCGAGCTATTGGTCTTTATCTTGAATATAAGCCTCCAATAGAAGAACTCAGATACTTCCTAGAGTTTCAAATTCCTCGAATATGGGCTCCTGTATATGACAGAAATCTTGCCCACCAAAGAAAGGAACCTGTTTGCCCATCTTTGCAATTCAGCATAATGGGGCAAAAACTCTATGTCAGCCAAGAGCAGCAAACTTTTTGCATGCAGATTACAGTTGGACGGAAGCCAGTGACGGGCTTGCGGTTATGTCTTGAAGGAAGCAAGCAGAATCGTCTTTGTATCCATCTTCAACACTTGGCATCTCTTCCTAAGATCCTTCTACCATATTGGGATACCCATGTAGCAATCGGTGCTCCAAAGTGGCAAGGCCCTGAGGAGCAGGATAGCCGATGGTTTGAGCCAGTGAAGTGGAAAAATTTCTCTCATGTGAGCACAACACCTATTGAGAATCCTGAAACCTTCATAGGCGATCTTTCTGGTGTCTACATAGTCACAGGAGCACAGCTTGGAGTGTGGGATTTCGGATCAAAAAATGTTTTGTACATGAAGCTATTATATTCCAGGCTGCCGGGCTGCACTATACGAAGATCTTTCTGGGATCATTCCCCAAATGAAAAATCAAAGAAAGGGACATCTACTAGTTTAAATTTCAATGCCGGTGACTCAAGTTCCGGTTCAAGAGAAAATGTTGCGGGTAACAAATTGACGAAATTTTTGGATATGTCTGAGATGAGCAAGGGGCCTCAAGATCATCCAGGTCATTGGTTGGTTACCGGTGGAAAGCTTGGTGTGGAAAAAGGAAAAATTGTCTTGAGAGTGAAATATTCGCTGCTAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

591

Amino Acids

66.28

Weight (kDa)

8.53

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 104 - 316 1.2e-28 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 748
AccBSI CCGCTC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 1413
AciI CCGC 5 cut(s) 226, 229, 546, 1171, 1622
AclWI GGATC 6 cut(s) 473, 486, 734, 1237, 1462, 1536
AcsI RAATTY 6 cut(s) 508, 679, 999, 1345, 1576, 1640
AcuI CTGAAG 1 cut(s) 319
AdeI CACNNNGTG 1 cut(s) 831
AfaI GTAC 4 cut(s) 45, 235, 517, 1472
AfiI CCNNNNNNNGG 4 cut(s) 225, 904, 1051, 1163
AgeI ACCGGT 1 cut(s) 1703
AhlI ACTAGT 1 cut(s) 1568
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 4 cut(s) 146, 191, 1490, 1684
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
Alw21I GWGCWC 5 cut(s) 144, 924, 1285, 1364, 1432
Alw26I GTCTC 1 cut(s) 798
AlwI GGATC 6 cut(s) 473, 486, 734, 1237, 1462, 1536
AlwNI CAGNNNCTG 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 930
AoxI GGCC 3 cut(s) 388, 1297, 1670
ApeKI GCWGC 7 cut(s) 17, 88, 890, 1117, 1495, 1503, 1759
ApoI RAATTY 6 cut(s) 508, 679, 999, 1345, 1576, 1640
ArsI GACNNNNNNTTYG 4 cut(s) 59, 91, 1627, 1659
AsiGI ACCGGT 1 cut(s) 1703
Asp700I GAANNNNTTC 1 cut(s) 1346
AspS9I GGNCC 3 cut(s) 849, 1298, 1670
AsuC2I CCSGG 2 cut(s) 829, 1500
AsuHPI GGTGA 4 cut(s) 133, 203, 919, 1601
AsuII TTCGAA 1 cut(s) 790
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 1 cut(s) 849
AxyI CCTNAGG 1 cut(s) 1302
BaeI ACNNNNGTAYC 4 cut(s) 507, 540, 1254, 1287
BamHI GGATCC 1 cut(s) 478
BanI GGYRCC 1 cut(s) 748
BanII GRGCYC 1 cut(s) 1018
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 5 cut(s) 144, 924, 1285, 1364, 1432
BbvI GCAGC 7 cut(s) 29, 75, 902, 1129, 1482, 1490, 1746
BccI CCATC 6 cut(s) 253, 380, 423, 1075, 1221, 1314
BceAI ACGGC 1 cut(s) 762
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 4 cut(s) 148, 193, 1492, 1686
BciVI GTATCC 2 cut(s) 1220, 1255
BcnI CCSGG 2 cut(s) 829, 1500
BcoDI GTCTC 1 cut(s) 798
BcuI ACTAGT 1 cut(s) 1568
BfaI CTAG 3 cut(s) 117, 989, 1569
BfmI CTRYAG 2 cut(s) 244, 885
BfuI GTATCC 2 cut(s) 1220, 1255
BglII AGATCT 1 cut(s) 1517
BisI GCNGC 9 cut(s) 18, 89, 227, 547, 891, 1118, 1496, 1504, 1760
BlsI GCNGC 9 cut(s) 19, 90, 228, 548, 892, 1119, 1497, 1505, 1761
BmcAI AGTACT 1 cut(s) 235
Bme1390I CCNGG 6 cut(s) 148, 193, 829, 1492, 1500, 1686
Bme18I GGWCC 1 cut(s) 849
BmeT110I CYCGRG 1 cut(s) 930
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 849, 1298, 1670
BmiI GGNNCC 6 cut(s) 152, 480, 750, 1017, 1056, 1671
BmrFI CCNGG 6 cut(s) 148, 193, 829, 1492, 1500, 1686
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 1238
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 573
BpmI CTGGAG 2 cut(s) 258, 840
Bpu14I TTCGAA 1 cut(s) 790
BpuEI CTTGAG 3 cut(s) 1579, 1659, 1762
BpuMI CCSGG 2 cut(s) 829, 1500
BsaJI CCNNGG 3 cut(s) 192, 489, 597
BsaWI WCCGGW 4 cut(s) 704, 846, 1601, 1703
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 904, 1051, 1163
Bse118I RCCGGY 2 cut(s) 1586, 1703
Bse1I ACTGG 7 cut(s) 57, 214, 219, 241, 577, 1157, 1331
Bse21I CCTNAGG 1 cut(s) 1302
BseBI CCWGG 4 cut(s) 148, 193, 1492, 1686
BseDI CCNNGG 3 cut(s) 192, 489, 597
BseGI GGATG 4 cut(s) 391, 485, 615, 1681
BseLI CCNNNNNNNGG 4 cut(s) 225, 904, 1051, 1163
BseMII CTCAG 4 cut(s) 657, 991, 1293, 1647
BseNI ACTGG 7 cut(s) 57, 214, 219, 241, 577, 1157, 1331
BseRI GAGGAG 3 cut(s) 152, 816, 1319
BseXI GCAGC 7 cut(s) 29, 75, 902, 1129, 1482, 1490, 1746
BsgI GTGCAG 1 cut(s) 1489
BshFI GGCC 3 cut(s) 390, 1299, 1672
BshNI GGYRCC 1 cut(s) 748
BshTI ACCGGT 1 cut(s) 1703
BsiHKAI GWGCWC 5 cut(s) 144, 924, 1285, 1364, 1432
BsiHKCI CYCGRG 1 cut(s) 930
BsiSI CCGG 7 cut(s) 705, 829, 847, 1499, 1587, 1602, 1704
BslFI GGGAC 1 cut(s) 1573
BslI CCNNNNNNNGG 4 cut(s) 225, 904, 1051, 1163
BsmAI GTCTC 1 cut(s) 798
BsmBI CGTCTC 1 cut(s) 798
BsmFI GGGAC 1 cut(s) 1573
BsnI GGCC 3 cut(s) 390, 1299, 1672
BsoBI CYCGRG 1 cut(s) 930
Bsp119I TTCGAA 1 cut(s) 790
Bsp1286I GDGCHC 6 cut(s) 144, 924, 1018, 1285, 1364, 1432
Bsp1407I TGTACA 2 cut(s) 43, 1470
Bsp143I GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 5 cut(s) 226, 229, 546, 1171, 1622
BspANI GGCC 3 cut(s) 390, 1299, 1672
BspCNI CTCAG 4 cut(s) 656, 990, 1294, 1648
BspLI GGNNCC 6 cut(s) 152, 480, 750, 1017, 1056, 1671
BspMAI CTGCAG 1 cut(s) 248
BspPI GGATC 6 cut(s) 473, 486, 734, 1237, 1462, 1536
BspT104I TTCGAA 1 cut(s) 790
BspT107I GGYRCC 1 cut(s) 748
BsrBI CCGCTC 1 cut(s) 226
BsrFI RCCGGY 2 cut(s) 1586, 1703
BsrGI TGTACA 2 cut(s) 43, 1470
BsrI ACTGG 7 cut(s) 57, 214, 219, 241, 577, 1157, 1331
BssAI RCCGGY 2 cut(s) 1586, 1703
BssECI CCNNGG 3 cut(s) 192, 489, 597
BssMI GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
BssT1I CCWWGG 2 cut(s) 489, 597
Bst2UI CCWGG 4 cut(s) 148, 193, 1492, 1686
Bst4CI ACNGT 4 cut(s) 719, 781, 844, 1144
Bst6I CTCTTC 3 cut(s) 52, 897, 1239
BstAPI GCANNNNNTGC 1 cut(s) 1280
BstAUI TGTACA 2 cut(s) 43, 1470
BstBI TTCGAA 1 cut(s) 790
BstC8I GCNNGC 4 cut(s) 496, 1133, 1169, 1193
BstDEI CTNAG 5 cut(s) 643, 977, 1239, 1302, 1656
BstDSI CCRYGG 1 cut(s) 597
BstEII GGTNACC 1 cut(s) 1699
BstENI CCTNNNNNAGG 1 cut(s) 902
BstF5I GGATG 4 cut(s) 391, 485, 615, 1681
BstKTI GATC 9 cut(s) 481, 606, 729, 1245, 1402, 1457, 1520, 1531, 1681
BstMAI GTCTC 1 cut(s) 798
BstMBI GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
BstMWI GCNNNNNNNGC 4 cut(s) 38, 47, 654, 1280
BstNI CCWGG 4 cut(s) 148, 193, 1492, 1686
BstNSI RCATGY 2 cut(s) 353, 1135
BstPI GGTNACC 1 cut(s) 1699
BstSCI CCNGG 6 cut(s) 146, 191, 827, 1490, 1498, 1684
BstSFI CTRYAG 2 cut(s) 244, 885
BstV1I GCAGC 7 cut(s) 29, 75, 902, 1129, 1482, 1490, 1746
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 4 cut(s) 478, 726, 1242, 1517
BstXI CCANNNNNNTGG 5 cut(s) 474, 490, 626, 1338, 1692
BstYI RGATCY 4 cut(s) 478, 726, 1242, 1517
Bsu36I CCTNAGG 1 cut(s) 1302
BsuI GTATCC 2 cut(s) 1220, 1255
BsuRI GGCC 3 cut(s) 390, 1299, 1672
BtgI CCRYGG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 4 cut(s) 391, 485, 615, 1681
BtsIMutI CAGTG 5 cut(s) 50, 226, 343, 1164, 1338
Cac8I GCNNGC 4 cut(s) 496, 1133, 1169, 1193
CaiI CAGNNNCTG 1 cut(s) 52
Cfr10I RCCGGY 2 cut(s) 1586, 1703
Cfr13I GGNCC 3 cut(s) 849, 1298, 1670
Csp6I GTAC 4 cut(s) 44, 234, 516, 1471
CspAI ACCGGT 1 cut(s) 1703
CviAII CATG 7 cut(s) 350, 521, 598, 1132, 1268, 1355, 1474
CviQI GTAC 4 cut(s) 44, 234, 516, 1471
DdeI CTNAG 5 cut(s) 643, 977, 1239, 1302, 1656
DpnI GATC 9 cut(s) 480, 605, 728, 1244, 1401, 1456, 1519, 1530, 1680
DpnII GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
DraI TTTAAA 2 cut(s) 423, 1575
DraIII CACNNNGTG 1 cut(s) 831
Eam1104I CTCTTC 3 cut(s) 52, 897, 1239
EarI CTCTTC 3 cut(s) 52, 897, 1239
EciI GGCGGA 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 489, 597
Eco24I GRGCYC 1 cut(s) 1018
Eco47I GGWCC 1 cut(s) 849
Eco57I CTGAAG 1 cut(s) 319
Eco81I CCTNAGG 1 cut(s) 1302
Eco88I CYCGRG 1 cut(s) 930
Eco91I GGTNACC 1 cut(s) 1699
EcoNI CCTNNNNNAGG 1 cut(s) 902
EcoO109I RGGNCCY 2 cut(s) 1298, 1670
EcoO65I GGTNACC 1 cut(s) 1699
EcoRII CCWGG 4 cut(s) 146, 191, 1490, 1684
EcoT14I CCWWGG 2 cut(s) 489, 597
EcoT38I GRGCYC 1 cut(s) 1018
ErhI CCWWGG 2 cut(s) 489, 597
Esp3I CGTCTC 1 cut(s) 798
FaeI CATG 7 cut(s) 353, 524, 601, 1135, 1271, 1358, 1477
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 1 cut(s) 1573
FatI CATG 7 cut(s) 349, 520, 597, 1131, 1267, 1354, 1473
FauI CCCGC 1 cut(s) 1615
FblI GTMKAC 1 cut(s) 1413
Fnu4HI GCNGC 9 cut(s) 18, 89, 227, 547, 891, 1118, 1496, 1504, 1760
FokI GGATG 4 cut(s) 398, 472, 602, 1668
FriOI GRGCYC 1 cut(s) 1018
Fsp4HI GCNGC 9 cut(s) 18, 89, 227, 547, 891, 1118, 1496, 1504, 1760
FspBI CTAG 3 cut(s) 117, 989, 1569
GluI GCNGC 9 cut(s) 18, 89, 227, 547, 891, 1118, 1496, 1504, 1760
GsuI CTGGAG 2 cut(s) 258, 840
HaeIII GGCC 3 cut(s) 390, 1299, 1672
HapII CCGG 7 cut(s) 705, 829, 847, 1499, 1587, 1602, 1704
Hin1II CATG 7 cut(s) 353, 524, 601, 1135, 1271, 1358, 1477
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HindIII AAGCTT 2 cut(s) 496, 1712
HinfI GANTC 6 cut(s) 240, 631, 701, 1198, 1378, 1592
HpaII CCGG 7 cut(s) 705, 829, 847, 1499, 1587, 1602, 1704
HphI GGTGA 4 cut(s) 133, 203, 919, 1601
Hpy166II GTNNAC 3 cut(s) 73, 594, 1414
Hpy188I TCNGA 5 cut(s) 636, 700, 980, 1454, 1657
Hpy188III TCNNGA 9 cut(s) 294, 607, 857, 950, 1181, 1382, 1608, 1676, 1741
Hpy8I GTNNAC 3 cut(s) 73, 594, 1414
HpyAV CCTTC 5 cut(s) 789, 909, 1178, 1256, 1399
HpyCH4III ACNGT 4 cut(s) 719, 781, 844, 1144
HpyCH4IV ACGT 2 cut(s) 69, 564
HpyCH4V TGCA 7 cut(s) 25, 246, 326, 1075, 1131, 1135, 1506
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 47, 654, 1280
HpyF3I CTNAG 5 cut(s) 643, 977, 1239, 1302, 1656
HpySE526I ACGT 2 cut(s) 69, 564
Hsp92II CATG 7 cut(s) 353, 524, 601, 1135, 1271, 1358, 1477
Kzo9I GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
LmnI GCTCC 6 cut(s) 139, 919, 1021, 1288, 1306, 1427
Lsp1109I GCAGC 7 cut(s) 29, 75, 902, 1129, 1482, 1490, 1746
LweI GCATC 1 cut(s) 1238
MaeI CTAG 3 cut(s) 117, 989, 1569
MaeII ACGT 2 cut(s) 69, 564
MaeIII GTNAC 8 cut(s) 438, 535, 775, 1159, 1420, 1589, 1625, 1699
MalI GATC 9 cut(s) 480, 605, 728, 1244, 1401, 1456, 1519, 1530, 1680
MbiI CCGCTC 1 cut(s) 226
MboI GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
MboII GAAGA 9 cut(s) 69, 573, 782, 804, 914, 983, 1208, 1226, 1527
MflI RGATCY 4 cut(s) 478, 726, 1242, 1517
MhlI GDGCHC 6 cut(s) 144, 924, 1018, 1285, 1364, 1432
MlyI GAGTC 2 cut(s) 249, 1586
MmeI TCCRAC 1 cut(s) 1126
MroXI GAANNNNTTC 1 cut(s) 1346
MseI TTAA 7 cut(s) 36, 201, 252, 273, 422, 567, 1574
MspI CCGG 7 cut(s) 705, 829, 847, 1499, 1587, 1602, 1704
MspR9I CCNGG 6 cut(s) 148, 193, 829, 1492, 1500, 1686
MvaI CCWGG 4 cut(s) 148, 193, 1492, 1686
MwoI GCNNNNNNNGC 4 cut(s) 38, 47, 654, 1280
NciI CCSGG 2 cut(s) 829, 1500
NcoI CCATGG 1 cut(s) 597
NdeII GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
NlaIII CATG 7 cut(s) 353, 524, 601, 1135, 1271, 1358, 1477
NlaIV GGNNCC 6 cut(s) 152, 480, 750, 1017, 1056, 1671
NmuCI GTSAC 4 cut(s) 775, 1159, 1420, 1589
NspI RCATGY 2 cut(s) 353, 1135
NspV TTCGAA 1 cut(s) 790
PaeI GCATGC 1 cut(s) 1135
PaeR7I CTCGAG 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 1346
PfeI GAWTC 4 cut(s) 631, 701, 1198, 1378
PinAI ACCGGT 1 cut(s) 1703
PkrI GCNGC 9 cut(s) 19, 90, 228, 548, 892, 1119, 1497, 1505, 1761
PleI GAGTC 2 cut(s) 248, 1586
PpsI GAGTC 2 cut(s) 248, 1586
Psp6I CCWGG 4 cut(s) 146, 191, 1490, 1684
PspEI GGTNACC 1 cut(s) 1699
PspGI CCWGG 4 cut(s) 146, 191, 1490, 1684
PspN4I GGNNCC 6 cut(s) 152, 480, 750, 1017, 1056, 1671
PspPI GGNCC 3 cut(s) 849, 1298, 1670
PspXI VCTCGAGB 1 cut(s) 930
PstI CTGCAG 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 4 cut(s) 478, 726, 1242, 1517
RsaI GTAC 4 cut(s) 45, 235, 517, 1472
RsaNI GTAC 4 cut(s) 44, 234, 516, 1471
SaqAI TTAA 7 cut(s) 36, 201, 252, 273, 422, 567, 1574
SatI GCNGC 9 cut(s) 18, 89, 227, 547, 891, 1118, 1496, 1504, 1760
Sau3AI GATC 9 cut(s) 478, 603, 726, 1242, 1399, 1454, 1517, 1528, 1678
Sau96I GGNCC 3 cut(s) 849, 1298, 1670
ScaI AGTACT 1 cut(s) 235
SchI GAGTC 2 cut(s) 249, 1586
ScrFI CCNGG 6 cut(s) 148, 193, 829, 1492, 1500, 1686
SduI GDGCHC 6 cut(s) 144, 924, 1018, 1285, 1364, 1432
SfaNI GCATC 1 cut(s) 1238
SfcI CTRYAG 2 cut(s) 244, 885
Sfr274I CTCGAG 1 cut(s) 930
SfuI TTCGAA 1 cut(s) 790
SinI GGWCC 1 cut(s) 849
SlaI CTCGAG 1 cut(s) 930
SmlI CTYRAG 4 cut(s) 930, 1594, 1674, 1741
SmoI CTYRAG 4 cut(s) 930, 1594, 1674, 1741
SpeI ACTAGT 1 cut(s) 1568
SphI GCATGC 1 cut(s) 1135
SsiI CCGC 5 cut(s) 226, 229, 546, 1171, 1622
SspI AATATT 1 cut(s) 1754
SspMI CTAG 3 cut(s) 117, 989, 1569
StyD4I CCNGG 6 cut(s) 146, 191, 827, 1490, 1498, 1684
StyI CCWWGG 2 cut(s) 489, 597
TaaI ACNGT 4 cut(s) 719, 781, 844, 1144
TaiI ACGT 2 cut(s) 72, 567
TaqI TCGA 4 cut(s) 264, 790, 931, 1006
TatI WGTACW 3 cut(s) 43, 233, 1470
TauI GCSGC 2 cut(s) 229, 549
TfiI GAWTC 4 cut(s) 631, 701, 1198, 1378
Tru1I TTAA 7 cut(s) 36, 201, 252, 273, 422, 567, 1574
Tru9I TTAA 7 cut(s) 36, 201, 252, 273, 422, 567, 1574
TscAI CASTG 5 cut(s) 57, 226, 343, 1164, 1338
TseFI GTSAC 4 cut(s) 775, 1159, 1420, 1589
TseI GCWGC 7 cut(s) 17, 88, 890, 1117, 1495, 1503, 1759
Tsp45I GTSAC 4 cut(s) 775, 1159, 1420, 1589
TspDTI ATGAA 4 cut(s) 692, 1381, 1490, 1557
TspGWI ACGGA 1 cut(s) 1165
TspRI CASTG 5 cut(s) 57, 226, 343, 1164, 1338
VpaK11BI GGWCC 1 cut(s) 849
XagI CCTNNNNNAGG 1 cut(s) 902
XapI RAATTY 6 cut(s) 508, 679, 999, 1345, 1576, 1640
XceI RCATGY 2 cut(s) 353, 1135
XhoI CTCGAG 1 cut(s) 930
XmiI GTMKAC 1 cut(s) 1413
XmnI GAANNNNTTC 1 cut(s) 1346
XspI CTAG 3 cut(s) 117, 989, 1569
ZrmI AGTACT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.