MD04G1173300.v1.1
ERF Family

MAC/Perforin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003665
Physical Location & Seq
Forward (+)
26477172 .. 26481337
4166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1173300.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGGGAGAAAATGCAGCAGCTTTGCACACAGCTGTGAACTCCGTACAGGCATTAGGAAGAGGTTTCGACGTGAACTTCGATACAAGGTTGCTATACTGTAAGGGAGCTGTGGGTACGAGGATTCTGGAGCTTGACGAGGATTGCACCAGGGATATCTGCCTATATGATGAGATAGTTGTACCGGGTGTTTCGAGGGACATCAGGCATTCGCAGGAGGCCGGCGGCCGGCAGAGCTCTGGAGTCTGCAGCTTCCATGAGATGGTTGAGTATTTCAATCAAAAGTCTAACGCATCTGGCAGCTTTCCTCTTGGGAGTTTCAACTTTGCATTTAGCTTCACTGGTTCAAAACATATTGATGCTGCAGCCACGAAGACTCTTTGTGTGGATGGATTTTATATTCCACTTGCTAAGGTCCAACTTTTCAAATCCCCAATAGTGTTGCAAGAACATGTTAAACAGGCTGTCCCAAGTAGTTGGGATCCAGCATCCTTGGCAAGCTTTATTGAAAATTTTGGAACCCATGTCATAACATCAGTAACAATTGGTGGGAAAGATGTAATTTACGTTAAACAACACCATTCATCCCCATTGTCTACCATGGAGATCAAAAACTATGTCCAGGACATTGGAACTCAAAGGTTCTCAGATACAGAAAGCAATGGAACTTCAGGTCAAATGAAATTCAATGATAAGGGTGGTGATCCTGGTTTATTCAACAGTCAAGGGATATATCCTCAACCCACTAGTGCACCATATCTTGCTGGGAAAGAAGATGTCACGGTCATTTTCAGAAGAAGGGGAGGAGACGATCTGGAACAAAACCACACCCAATGGGCAAGAACTGTCTGTTCCTCTCCAGATGTAATCGAGATGACATTTTTTCCTATAGCGGCCCTACTGGAAGAGATACCTGGAAAAGAGCACCTGACTCGAGCTATTGGTCTTTACCTTGAATATAAGCCCCCAATAGAAGAGCTGAGATATTTCCTGGAGTTTCAAATTTCTCGAATTTGGGCTCCTGTATATGATAGAAATATTGGGCATCAAAGAAAGGAACCTGTTTGCCCATCCTTGCAATTCAGCATAATGGGGCAAAAGCTTTATGTCAGCCAAGAACAGGTTACTGTTAAACGGAAGCCAGTCACAGGCCTACGGTTGTATCTAGAAGGAAGCAAGCAGAATCGACTTTGTATCCATCTTCAGCACTTAGCATCTCTTCCAAAGATCCTTCAACCATATTGGGACAGTGATGTGGCAATTGGTGCTCCCAAGTGGCAAGGACCTGAAGAGCAAGACAGCCGATGGTTTGAACCAGTAAAATGGAAAAACTTCTCTCATGTGAGCACTGCACCAATTGAGAACCCTGAAACCTTCATAGGGGACCTCTCTGGTGTCTACATAGTCACTGGTGCACAGCTCGGAGTGTGGGACTTTGGATCGAGAAATGTCTTGTACATGAAGCTATCATATTCTAGGCTGCCAGGCTGCACCGTACGGAGATCCTTGTGGGATCATACGCCAAATGAAAAGTCAAAGAAAGCAACATCCACTGGTGACTCAAGTTCAGGTTCAAGAGAACATGTCGCTGGCAACAAGTTGGTGAAGGTTGTGGACATGTCGGAGATGAGCAAGGGACCACGAGACCATCCTGGTCATTGGCTGGTTACCGGTGGAAAGCTTGGCGTGGAGAGAGGGAAAATTGTTTTAAGAGTGAAATATTCGTTACTAAGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

579

Amino Acids

64.42

Weight (kDa)

7.64

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 104 - 316 3.3e-28 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 259
AccI GTMKAC 2 cut(s) 593, 1395
AciI CCGC 2 cut(s) 222, 890
AclWI GGATC 7 cut(s) 473, 486, 695, 1219, 1444, 1494, 1518
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 4 cut(s) 508, 680, 999, 1008
AcuI CTGAAG 3 cut(s) 651, 1184, 1305
AfaI GTAC 5 cut(s) 45, 115, 180, 1454, 1494
AfiI CCNNNNNNNGG 5 cut(s) 225, 259, 1117, 1145, 1377
AflIII ACRYGT 3 cut(s) 448, 1579, 1614
AgeI ACCGGT 1 cut(s) 1667
AhlI ACTAGT 1 cut(s) 743
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 7 cut(s) 146, 618, 703, 910, 987, 1480, 1648
AleI CACNNNNGTG 1 cut(s) 32
Alw21I GWGCWC 6 cut(s) 236, 751, 924, 1267, 1346, 1414
Alw26I GTCTC 2 cut(s) 798, 1635
Alw44I GTGCAC 2 cut(s) 747, 1410
AlwI GGATC 7 cut(s) 473, 486, 695, 1219, 1444, 1494, 1518
Ama87I CYCGRG 1 cut(s) 930
AoxI GGCC 4 cut(s) 216, 223, 891, 1147
ApaLI GTGCAC 2 cut(s) 747, 1410
ApeKI GCWGC 8 cut(s) 14, 17, 246, 297, 359, 362, 1477, 1485
ApoI RAATTY 4 cut(s) 508, 680, 999, 1008
ArsI GACNNNNNNTTYG 6 cut(s) 59, 91, 600, 632, 1514, 1546
AsiGI ACCGGT 1 cut(s) 1667
Asp700I GAANNNNTTC 1 cut(s) 1328
AspS9I GGNCC 5 cut(s) 412, 892, 1280, 1381, 1634
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 3 cut(s) 710, 1565, 1612
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 4 cut(s) 412, 1280, 1381, 1634
BaeGI GKGCMC 2 cut(s) 751, 1414
BaeI ACNNNNGTAYC 2 cut(s) 1142, 1175
BamHI GGATCC 1 cut(s) 478
BanII GRGCYC 2 cut(s) 236, 1018
BauI CACGAG 1 cut(s) 1638
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 6 cut(s) 236, 751, 924, 1267, 1346, 1414
BbvI GCAGC 8 cut(s) 26, 29, 258, 309, 346, 374, 1464, 1472
BccI CCATC 6 cut(s) 253, 380, 1075, 1203, 1296, 1653
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 7 cut(s) 148, 620, 705, 912, 989, 1482, 1650
BciVI GTATCC 1 cut(s) 1202
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 2 cut(s) 798, 1635
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 3 cut(s) 744, 1163, 1473
BfmI CTRYAG 3 cut(s) 244, 360, 885
BfuI GTATCC 1 cut(s) 1202
Bme1390I CCNGG 8 cut(s) 148, 183, 620, 705, 912, 989, 1482, 1650
Bme18I GGWCC 4 cut(s) 412, 1280, 1381, 1634
BmeT110I CYCGRG 1 cut(s) 930
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 5 cut(s) 412, 892, 1280, 1381, 1634
BmiI GGNNCC 6 cut(s) 480, 517, 1017, 1056, 1382, 1635
BmrFI CCNGG 8 cut(s) 148, 183, 620, 705, 912, 989, 1482, 1650
BmsI GCATC 5 cut(s) 299, 346, 494, 1051, 1220
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 4 cut(s) 146, 258, 840, 1010
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 1543
BpuMI CCSGG 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 1635
BsaJI CCNNGG 3 cut(s) 147, 489, 597
BsaWI WCCGGW 1 cut(s) 1667
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 259, 1117, 1145, 1377
Bse118I RCCGGY 3 cut(s) 218, 225, 1667
Bse1I ACTGG 6 cut(s) 343, 903, 1139, 1313, 1411, 1555
Bse3DI GCAATG 1 cut(s) 664
BseBI CCWGG 7 cut(s) 148, 620, 705, 912, 989, 1482, 1650
BseDI CCNNGG 3 cut(s) 147, 489, 597
BseGI GGATG 6 cut(s) 391, 485, 581, 1067, 1544, 1645
BseLI CCNNNNNNNGG 5 cut(s) 225, 259, 1117, 1145, 1377
BseMI GCAATG 1 cut(s) 664
BseMII CTCAG 2 cut(s) 657, 968
BseNI ACTGG 6 cut(s) 343, 903, 1139, 1313, 1411, 1555
BseRI GAGGAG 1 cut(s) 816
BseSI GKGCMC 2 cut(s) 751, 1414
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 8 cut(s) 26, 29, 258, 309, 346, 374, 1464, 1472
BseYI CCCAGC 1 cut(s) 761
BsgI GTGCAG 2 cut(s) 1332, 1471
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 4 cut(s) 218, 225, 893, 1149
BshTI ACCGGT 1 cut(s) 1667
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 6 cut(s) 236, 751, 924, 1267, 1346, 1414
BsiHKCI CYCGRG 1 cut(s) 930
BsiSI CCGG 4 cut(s) 182, 219, 226, 1668
BsiWI CGTACG 1 cut(s) 1492
BslFI GGGAC 6 cut(s) 209, 449, 1256, 1394, 1442, 1647
BslI CCNNNNNNNGG 5 cut(s) 225, 259, 1117, 1145, 1377
BsmAI GTCTC 2 cut(s) 798, 1635
BsmBI CGTCTC 1 cut(s) 798
BsmFI GGGAC 6 cut(s) 209, 449, 1256, 1394, 1442, 1647
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 4 cut(s) 218, 225, 893, 1149
Bso31I GGTCTC 1 cut(s) 1635
BsoBI CYCGRG 1 cut(s) 930
Bsp1286I GDGCHC 7 cut(s) 236, 751, 924, 1018, 1267, 1346, 1414
Bsp1407I TGTACA 1 cut(s) 1452
Bsp143I GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 2 cut(s) 222, 890
BspANI GGCC 4 cut(s) 218, 225, 893, 1149
BspCNI CTCAG 2 cut(s) 656, 969
BspLI GGNNCC 6 cut(s) 480, 517, 1017, 1056, 1382, 1635
BspMAI CTGCAG 2 cut(s) 248, 364
BspPI GGATC 7 cut(s) 473, 486, 695, 1219, 1444, 1494, 1518
BspQI GCTCTTC 2 cut(s) 966, 1281
BspTNI GGTCTC 1 cut(s) 1635
BsrDI GCAATG 1 cut(s) 664
BsrFI RCCGGY 3 cut(s) 218, 225, 1667
BsrGI TGTACA 1 cut(s) 1452
BsrI ACTGG 6 cut(s) 343, 903, 1139, 1313, 1411, 1555
BssAI RCCGGY 3 cut(s) 218, 225, 1667
BssECI CCNNGG 3 cut(s) 147, 489, 597
BssMI GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
BssSI CACGAG 1 cut(s) 1638
BssT1I CCWWGG 2 cut(s) 489, 597
Bst2BI CACGAG 1 cut(s) 1638
Bst2UI CCWGG 7 cut(s) 148, 620, 705, 912, 989, 1482, 1650
Bst4CI ACNGT 8 cut(s) 98, 719, 781, 844, 1126, 1155, 1247, 1492
Bst6I CTCTTC 5 cut(s) 52, 897, 966, 1221, 1281
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstAUI TGTACA 1 cut(s) 1452
BstC8I GCNNGC 5 cut(s) 220, 227, 496, 1175, 1588
BstDEI CTNAG 5 cut(s) 408, 643, 977, 1207, 1727
BstDSI CCRYGG 1 cut(s) 597
BstEII GGTNACC 1 cut(s) 1663
BstF5I GGATG 6 cut(s) 391, 485, 581, 1067, 1544, 1645
BstKTI GATC 8 cut(s) 481, 606, 703, 811, 1227, 1439, 1502, 1513
BstMAI GTCTC 2 cut(s) 798, 1635
BstMBI GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 3 cut(s) 231, 491, 1262
BstNI CCWGG 7 cut(s) 148, 620, 705, 912, 989, 1482, 1650
BstNSI RCATGY 3 cut(s) 452, 1583, 1618
BstPI GGTNACC 1 cut(s) 1663
BstSCI CCNGG 8 cut(s) 146, 181, 618, 703, 910, 987, 1480, 1648
BstSFI CTRYAG 3 cut(s) 244, 360, 885
BstSLI GKGCMC 2 cut(s) 751, 1414
BstV1I GCAGC 8 cut(s) 26, 29, 258, 309, 346, 374, 1464, 1472
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 3 cut(s) 478, 1224, 1499
BstXI CCANNNNNNTGG 3 cut(s) 474, 626, 1320
BstYI RGATCY 3 cut(s) 478, 1224, 1499
BstZI CGGCCG 1 cut(s) 223
BsuI GTATCC 1 cut(s) 1202
BsuRI GGCC 4 cut(s) 218, 225, 893, 1149
BtgI CCRYGG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 6 cut(s) 391, 485, 581, 1067, 1544, 1645
BtsI GCAGTG 1 cut(s) 1344
BtsIMutI CAGTG 5 cut(s) 336, 1252, 1344, 1404, 1548
Cac8I GCNNGC 5 cut(s) 220, 227, 496, 1175, 1588
Cfr10I RCCGGY 3 cut(s) 218, 225, 1667
Cfr13I GGNCC 5 cut(s) 412, 892, 1280, 1381, 1634
Csp6I GTAC 5 cut(s) 44, 114, 179, 1453, 1493
CspAI ACCGGT 1 cut(s) 1667
CviAII CATG 8 cut(s) 254, 449, 521, 598, 1337, 1456, 1580, 1615
CviQI GTAC 5 cut(s) 44, 114, 179, 1453, 1493
DdeI CTNAG 5 cut(s) 408, 643, 977, 1207, 1727
DpnI GATC 8 cut(s) 480, 605, 702, 810, 1226, 1438, 1501, 1512
DpnII GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
EaeI YGGCCR 1 cut(s) 223
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 5 cut(s) 52, 897, 966, 1221, 1281
EarI CTCTTC 5 cut(s) 52, 897, 966, 1221, 1281
Ecl136II GAGCTC 1 cut(s) 234
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 489, 597
Eco147I AGGCCT 1 cut(s) 1149
Eco24I GRGCYC 2 cut(s) 236, 1018
Eco31I GGTCTC 1 cut(s) 1635
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 4 cut(s) 412, 1280, 1381, 1634
Eco52I CGGCCG 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 234
Eco57I CTGAAG 3 cut(s) 651, 1184, 1305
Eco88I CYCGRG 1 cut(s) 930
Eco91I GGTNACC 1 cut(s) 1663
EcoICRI GAGCTC 1 cut(s) 234
EcoO109I RGGNCCY 2 cut(s) 1280, 1381
EcoO65I GGTNACC 1 cut(s) 1663
EcoRII CCWGG 7 cut(s) 146, 618, 703, 910, 987, 1480, 1648
EcoRV GATATC 1 cut(s) 154
EcoT14I CCWWGG 2 cut(s) 489, 597
EcoT38I GRGCYC 2 cut(s) 236, 1018
ErhI CCWWGG 2 cut(s) 489, 597
Esp3I CGTCTC 1 cut(s) 798
FaeI CATG 8 cut(s) 257, 452, 524, 601, 1340, 1459, 1583, 1618
FaqI GGGAC 6 cut(s) 209, 449, 1256, 1394, 1442, 1647
FatI CATG 8 cut(s) 253, 448, 520, 597, 1336, 1455, 1579, 1614
FblI GTMKAC 2 cut(s) 593, 1395
FokI GGATG 6 cut(s) 398, 472, 568, 1054, 1531, 1632
FriOI GRGCYC 2 cut(s) 236, 1018
FspBI CTAG 3 cut(s) 744, 1163, 1473
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 4 cut(s) 146, 258, 840, 1010
HaeIII GGCC 4 cut(s) 218, 225, 893, 1149
HapII CCGG 4 cut(s) 182, 219, 226, 1668
Hin1II CATG 8 cut(s) 257, 452, 524, 601, 1340, 1459, 1583, 1618
HindIII AAGCTT 3 cut(s) 496, 1097, 1676
HinfI GANTC 6 cut(s) 121, 240, 373, 928, 1180, 1556
HpaII CCGG 4 cut(s) 182, 219, 226, 1668
HphI GGTGA 3 cut(s) 710, 1565, 1612
Hpy166II GTNNAC 7 cut(s) 37, 73, 594, 749, 1396, 1412, 1612
Hpy188I TCNGA 4 cut(s) 646, 791, 1421, 1621
Hpy188III TCNNGA 9 cut(s) 125, 237, 812, 857, 868, 1005, 1163, 1440, 1572
Hpy8I GTNNAC 7 cut(s) 37, 73, 594, 749, 1396, 1412, 1612
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 5 cut(s) 789, 1160, 1238, 1381, 1597
HpyCH4III ACNGT 8 cut(s) 98, 719, 781, 844, 1126, 1155, 1247, 1492
HpyCH4IV ACGT 2 cut(s) 69, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 491, 1262
HpyF3I CTNAG 5 cut(s) 408, 643, 977, 1207, 1727
HpySE526I ACGT 2 cut(s) 69, 564
Hsp92II CATG 8 cut(s) 257, 452, 524, 601, 1340, 1459, 1583, 1618
KroI GCCGGC 2 cut(s) 218, 225
KroNI GCCGGC 2 cut(s) 220, 227
Kzo9I GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
LguI GCTCTTC 2 cut(s) 966, 1281
LmnI GCTCC 4 cut(s) 104, 127, 1021, 1270
Lsp1109I GCAGC 8 cut(s) 26, 29, 258, 309, 346, 374, 1464, 1472
LweI GCATC 5 cut(s) 299, 346, 494, 1051, 1220
MaeI CTAG 3 cut(s) 744, 1163, 1473
MaeII ACGT 2 cut(s) 69, 564
MaeIII GTNAC 8 cut(s) 535, 775, 1120, 1141, 1402, 1553, 1663, 1722
MalI GATC 8 cut(s) 480, 605, 702, 810, 1226, 1438, 1501, 1512
MboI GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
MboII GAAGA 9 cut(s) 69, 382, 782, 804, 914, 983, 1190, 1208, 1298
MfeI CAATTG 3 cut(s) 540, 1257, 1353
MflI RGATCY 3 cut(s) 478, 1224, 1499
MhlI GDGCHC 7 cut(s) 236, 751, 924, 1018, 1267, 1346, 1414
MlyI GAGTC 4 cut(s) 249, 367, 922, 1550
MmeI TCCRAC 2 cut(s) 439, 1599
MroNI GCCGGC 2 cut(s) 218, 225
MroXI GAANNNNTTC 1 cut(s) 1328
MseI TTAA 4 cut(s) 453, 567, 1128, 1706
MslI CAYNNNNRTG 2 cut(s) 32, 354
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 4 cut(s) 182, 219, 226, 1668
MspR9I CCNGG 8 cut(s) 148, 183, 620, 705, 912, 989, 1482, 1650
MunI CAATTG 3 cut(s) 540, 1257, 1353
Mva1269I GAATGC 1 cut(s) 205
MvaI CCWGG 7 cut(s) 148, 620, 705, 912, 989, 1482, 1650
MwoI GCNNNNNNNGC 3 cut(s) 231, 491, 1262
NaeI GCCGGC 2 cut(s) 220, 227
NciI CCSGG 1 cut(s) 183
NcoI CCATGG 1 cut(s) 597
NdeII GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
NgoMIV GCCGGC 2 cut(s) 218, 225
NlaIII CATG 8 cut(s) 257, 452, 524, 601, 1340, 1459, 1583, 1618
NlaIV GGNNCC 6 cut(s) 480, 517, 1017, 1056, 1382, 1635
NmuCI GTSAC 4 cut(s) 775, 1141, 1402, 1553
NspI RCATGY 3 cut(s) 452, 1583, 1618
OliI CACNNNNGTG 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 930
PceI AGGCCT 1 cut(s) 1149
PciI ACATGT 3 cut(s) 448, 1579, 1614
PciSI GCTCTTC 2 cut(s) 966, 1281
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 205
PdiI GCCGGC 2 cut(s) 220, 227
PdmI GAANNNNTTC 1 cut(s) 1328
PfeI GAWTC 2 cut(s) 121, 1180
Pfl23II CGTACG 1 cut(s) 1492
PflMI CCANNNNNTGG 1 cut(s) 259
PfoI TCCNGGA 2 cut(s) 618, 987
PinAI ACCGGT 1 cut(s) 1667
PleI GAGTC 4 cut(s) 248, 367, 922, 1550
PpsI GAGTC 4 cut(s) 248, 367, 922, 1550
PpuMI RGGWCCY 2 cut(s) 1280, 1381
PscI ACATGT 3 cut(s) 448, 1579, 1614
Psp124BI GAGCTC 1 cut(s) 236
Psp5II RGGWCCY 2 cut(s) 1280, 1381
Psp6I CCWGG 7 cut(s) 146, 618, 703, 910, 987, 1480, 1648
PspEI GGTNACC 1 cut(s) 1663
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 7 cut(s) 146, 618, 703, 910, 987, 1480, 1648
PspLI CGTACG 1 cut(s) 1492
PspN4I GGNNCC 6 cut(s) 480, 517, 1017, 1056, 1382, 1635
PspPI GGNCC 5 cut(s) 412, 892, 1280, 1381, 1634
PspPPI RGGWCCY 2 cut(s) 1280, 1381
PspXI VCTCGAGB 1 cut(s) 930
PstI CTGCAG 2 cut(s) 248, 364
PsuI RGATCY 3 cut(s) 478, 1224, 1499
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 5 cut(s) 45, 115, 180, 1454, 1494
RsaNI GTAC 5 cut(s) 44, 114, 179, 1453, 1493
RseI CAYNNNNRTG 2 cut(s) 32, 354
SacI GAGCTC 1 cut(s) 236
SapI GCTCTTC 2 cut(s) 966, 1281
SaqAI TTAA 4 cut(s) 453, 567, 1128, 1706
Sau3AI GATC 8 cut(s) 478, 603, 700, 808, 1224, 1436, 1499, 1510
Sau96I GGNCC 5 cut(s) 412, 892, 1280, 1381, 1634
SchI GAGTC 4 cut(s) 249, 367, 922, 1550
ScrFI CCNGG 8 cut(s) 148, 183, 620, 705, 912, 989, 1482, 1650
SduI GDGCHC 7 cut(s) 236, 751, 924, 1018, 1267, 1346, 1414
SfaNI GCATC 5 cut(s) 299, 346, 494, 1051, 1220
SfcI CTRYAG 3 cut(s) 244, 360, 885
Sfr274I CTCGAG 1 cut(s) 930
SinI GGWCC 4 cut(s) 412, 1280, 1381, 1634
SlaI CTCGAG 1 cut(s) 930
SmiMI CAYNNNNRTG 2 cut(s) 32, 354
SmlI CTYRAG 2 cut(s) 930, 1558
SmoI CTYRAG 2 cut(s) 930, 1558
SpeI ACTAGT 1 cut(s) 743
SseBI AGGCCT 1 cut(s) 1149
SsiI CCGC 2 cut(s) 222, 890
SspI AATATT 2 cut(s) 1036, 1718
SspMI CTAG 3 cut(s) 744, 1163, 1473
SstI GAGCTC 1 cut(s) 236
StuI AGGCCT 1 cut(s) 1149
StyD4I CCNGG 8 cut(s) 146, 181, 618, 703, 910, 987, 1480, 1648
StyI CCWWGG 2 cut(s) 489, 597
TaaI ACNGT 8 cut(s) 98, 719, 781, 844, 1126, 1155, 1247, 1492
TaiI ACGT 2 cut(s) 72, 567
TaqI TCGA 8 cut(s) 66, 78, 191, 867, 931, 1006, 1183, 1439
TatI WGTACW 1 cut(s) 1452
TauI GCSGC 2 cut(s) 225, 893
TfiI GAWTC 2 cut(s) 121, 1180
Tru1I TTAA 4 cut(s) 453, 567, 1128, 1706
Tru9I TTAA 4 cut(s) 453, 567, 1128, 1706
TscAI CASTG 5 cut(s) 343, 1252, 1351, 1411, 1555
TseFI GTSAC 4 cut(s) 775, 1141, 1402, 1553
TseI GCWGC 8 cut(s) 14, 17, 246, 297, 359, 362, 1477, 1485
Tsp45I GTSAC 4 cut(s) 775, 1141, 1402, 1553
TspDTI ATGAA 5 cut(s) 570, 692, 1363, 1472, 1539
TspGWI ACGGA 3 cut(s) 31, 1147, 1510
TspRI CASTG 5 cut(s) 343, 1252, 1351, 1411, 1555
Van91I CCANNNNNTGG 1 cut(s) 259
VneI GTGCAC 2 cut(s) 747, 1410
VpaK11BI GGWCC 4 cut(s) 412, 1280, 1381, 1634
XapI RAATTY 4 cut(s) 508, 680, 999, 1008
XbaI TCTAGA 1 cut(s) 1162
XceI RCATGY 3 cut(s) 452, 1583, 1618
XhoI CTCGAG 1 cut(s) 930
XmiI GTMKAC 2 cut(s) 593, 1395
XmnI GAANNNNTTC 1 cut(s) 1328
XspI CTAG 3 cut(s) 744, 1163, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.