Rh3AG102800

MACPF domain-containing protein CAD1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0003665
Physical Location & Seq
Reverse (-)
8535865 .. 8541384
5520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG102800.1

Sequence Viewer

Length: 1491 bp
ATGGTTGAGTATTTTAATCAAAAGTCTAATATATCTGGAGGCTATCCTGTGGGGAGTTTCAATTTTGCATTTAGCTTCAGCGGTTCAAAGCATGTTGATATGGCAGGAACAAAGACACTTTCTATGGATGGCCTCTATGTTCCACTTGCTAAGATCCATCTTTTAATATCCCCACTAATGTTACAACAAAATGTGAAACAGGCTGTCCCAACTTGTTGGGATCCCAAATCCTTGGCAAGCTTTATTGAAAATTTTGGTACTCATGTCATAACATCTGTAACTATTGGTGGCAAAGATGTAATTTACGTTAAACAACACCAGTCTTCTCCTTTGTCAACCATGGAGATCAAGAACTACATCCAAGACATTGGAAATCAGAGGTTCTCAAACACAGAAAGCAATACAAGTTCAGTTCAAATGAATTTCAAGGATAAGGGGTCTGATTCCGGTTTATTCAACAGTCAGGGGATATACCCTCAACCCACCACTGCACCACATCTTGCTGGGAAAGAAGATGTGACTGTCATTTTCCGAAGAAGGGGAGGAGATGATCTGGAACAAAACCACACCCGGTGGGCAAGAACTGTCCGGTCCTCTCCAGATGTTATCGAGATGACATTTTTTCCAATAACAGCTCTCCTTGAAGGGGTAATTGGAAGGGAGCACCTTACTCGAGCTATTGGCCTTTACCTTGAATATAAACCTCCAATAGAAGAACTGAGATACTTCCTAGAGTTTCAAATTTCTCGAATATGGGCTCCTGTATATGACAGAAATCCTACCCACCAAAGAAAGGAACCTGTTTGCCCATCTTTGCAATTCAGCATAATGGGGCAAAAACTCTATGTCAGCCAAGAGCAGATAACAGTTGGACGGAAGCCAGTGACAGGCTTGCGGTTATGTCTCGAAGGAAGCAAGCAGAATCGTCTTTGTATCCATCTTCAACACTTGGCCTCTCTTCCAAAGATCCTTCTACCATATTGGGATTCCCATGTAGCAATTGGTGCTCCAAAGTGGCAAGGACCTGAGGAGCAGGATAGCCGATGGTTCGAGCCAGTGAAGTGGAAAAACTTCTCTCATGTGAGCACAACACCTATTGAGAACCCTGAAACCTTTATAGGCGATCTCTCTGGTGTCTACATCGTCACAGGAGCTCAGCTTGGAGTGTGGGATTTCGGATCAAAAAATGTTTTGTACATGAAGCTATTATATTCCAGGCTGCCTGGCTGCACCATACGAAGATCCTTGTGGGACCATTCCCCAAATGAAAAGTCAAAGAAAGGGACGTCTGCTAGTTTAAATGTCAATGCCAGTGACTCAAGTTCCGGTTCACATGTTGCGGGTAACAAGTTGATGAAATATTTGGATATTTCCGAGATGAGCAAGGGGCCACAAGATCATCCAGGTCATTGGTTGGTTACTGGTGGAAAGCTTGGTGTGGAAAAAGGAAAAATTGTCTTAAGAGTGAAATATTCGCTGCTAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

496

Amino Acids

55.64

Weight (kDa)

9.13

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 18 - 230 2.9e-29 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1289
AccI GTMKAC 1 cut(s) 1137
AciI CCGC 3 cut(s) 81, 895, 1340
AclWI GGATC 6 cut(s) 148, 215, 228, 961, 1186, 1236
AcsI RAATTY 3 cut(s) 250, 421, 741
AcuI CTGAAG 1 cut(s) 61
AcyI GRCGYC 1 cut(s) 1286
AdeI CACNNNGTG 1 cut(s) 573
AfaI GTAC 2 cut(s) 259, 1196
AfiI CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
AflII CTTAAG 1 cut(s) 1459
AflIII ACRYGT 1 cut(s) 1333
AjnI CCWGG 3 cut(s) 1214, 1222, 1402
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 8 cut(s) 75, 240, 635, 677, 1154, 1159, 1204, 1432
AluI AGCT 8 cut(s) 75, 240, 635, 677, 1154, 1159, 1204, 1432
Alw21I GWGCWC 4 cut(s) 666, 1009, 1088, 1156
Alw26I GTCTC 1 cut(s) 908
AlwI GGATC 6 cut(s) 148, 215, 228, 961, 1186, 1236
Ama87I CYCGRG 1 cut(s) 672
AoxI GGCC 4 cut(s) 130, 682, 951, 1388
ApeKI GCWGC 3 cut(s) 1219, 1227, 1477
ApoI RAATTY 3 cut(s) 250, 421, 741
ArsI GACNNNNNNTTYG 2 cut(s) 1256, 1288
Asp700I GAANNNNTTC 1 cut(s) 1070
AspS9I GGNCC 4 cut(s) 591, 1022, 1252, 1388
AsuC2I CCSGG 1 cut(s) 571
AvaI CYCGRG 1 cut(s) 672
AvaII GGWCC 3 cut(s) 591, 1022, 1252
AxyI CCTNAGG 1 cut(s) 1026
BaeI ACNNNNGTAYC 2 cut(s) 249, 282
BamHI GGATCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 760, 1156
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 4 cut(s) 666, 1009, 1088, 1156
BbvI GCAGC 3 cut(s) 1206, 1214, 1464
BccI CCATC 5 cut(s) 122, 165, 817, 945, 1038
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 3 cut(s) 1216, 1224, 1404
BciVI GTATCC 1 cut(s) 944
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 908
BfaI CTAG 2 cut(s) 731, 1293
BfrI CTTAAG 1 cut(s) 1459
BfuI GTATCC 1 cut(s) 944
BisI GCNGC 3 cut(s) 1220, 1228, 1478
BlpI GCTNAGC 1 cut(s) 1155
BlsI GCNGC 3 cut(s) 1221, 1229, 1479
Bme1390I CCNGG 4 cut(s) 571, 1216, 1224, 1404
Bme18I GGWCC 3 cut(s) 591, 1022, 1252
BmeT110I CYCGRG 1 cut(s) 672
BmgT120I GGNCC 4 cut(s) 591, 1022, 1252, 1388
BmiI GGNNCC 5 cut(s) 222, 759, 798, 1253, 1389
BmrFI CCNGG 4 cut(s) 571, 1216, 1224, 1404
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 2 cut(s) 57, 582
Bpu1102I GCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 1 cut(s) 1303
BpuMI CCSGG 1 cut(s) 571
BsaHI GRCGYC 1 cut(s) 1286
BsaJI CCNNGG 2 cut(s) 231, 339
BsaWI WCCGGW 3 cut(s) 446, 588, 1325
Bsc4I CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
Bse1I ACTGG 5 cut(s) 319, 881, 1055, 1311, 1426
Bse21I CCTNAGG 1 cut(s) 1026
BseBI CCWGG 3 cut(s) 1216, 1224, 1404
BseDI CCNNGG 2 cut(s) 231, 339
BseGI GGATG 3 cut(s) 133, 357, 1399
BseLI CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
BseMII CTCAG 3 cut(s) 710, 1017, 1169
BseNI ACTGG 5 cut(s) 319, 881, 1055, 1311, 1426
BseRI GAGGAG 2 cut(s) 558, 1043
BseXI GCAGC 3 cut(s) 1206, 1214, 1464
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 2 cut(s) 474, 1213
BshFI GGCC 4 cut(s) 132, 684, 953, 1390
BsiHKAI GWGCWC 4 cut(s) 666, 1009, 1088, 1156
BsiHKCI CYCGRG 1 cut(s) 672
BsiSI CCGG 4 cut(s) 447, 571, 589, 1326
BslFI GGGAC 3 cut(s) 191, 1265, 1297
BslI CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
BsmAI GTCTC 1 cut(s) 908
BsmFI GGGAC 3 cut(s) 191, 1265, 1297
BsnI GGCC 4 cut(s) 132, 684, 953, 1390
BsoBI CYCGRG 1 cut(s) 672
Bsp1286I GDGCHC 5 cut(s) 666, 760, 1009, 1088, 1156
Bsp1407I TGTACA 1 cut(s) 1194
Bsp143I GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
Bsp1720I GCTNAGC 1 cut(s) 1155
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 81, 895, 1340
BspANI GGCC 4 cut(s) 132, 684, 953, 1390
BspCNI CTCAG 3 cut(s) 711, 1018, 1168
BspLI GGNNCC 5 cut(s) 222, 759, 798, 1253, 1389
BspPI GGATC 6 cut(s) 148, 215, 228, 961, 1186, 1236
BspTI CTTAAG 1 cut(s) 1459
BsrGI TGTACA 1 cut(s) 1194
BsrI ACTGG 5 cut(s) 319, 881, 1055, 1311, 1426
BssECI CCNNGG 2 cut(s) 231, 339
BssMI GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
BssNI GRCGYC 1 cut(s) 1286
BssT1I CCWWGG 2 cut(s) 231, 339
Bst2UI CCWGG 3 cut(s) 1216, 1224, 1404
Bst4CI ACNGT 4 cut(s) 461, 523, 586, 868
Bst6I CTCTTC 1 cut(s) 963
BstACI GRCGYC 1 cut(s) 1286
BstAFI CTTAAG 1 cut(s) 1459
BstAPI GCANNNNNTGC 1 cut(s) 1004
BstAUI TGTACA 1 cut(s) 1194
BstC8I GCNNGC 3 cut(s) 238, 893, 917
BstDEI CTNAG 4 cut(s) 150, 719, 1026, 1155
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 133, 357, 1399
BstKTI GATC 9 cut(s) 156, 223, 348, 553, 969, 1126, 1181, 1244, 1399
BstMAI GTCTC 1 cut(s) 908
BstMBI GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
BstMWI GCNNNNNNNGC 1 cut(s) 1004
BstNI CCWGG 3 cut(s) 1216, 1224, 1404
BstNSI RCATGY 2 cut(s) 95, 1337
BstSCI CCNGG 4 cut(s) 569, 1214, 1222, 1402
BstV1I GCAGC 3 cut(s) 1206, 1214, 1464
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 4 cut(s) 153, 220, 966, 1241
BstXI CCANNNNNNTGG 5 cut(s) 216, 232, 368, 1062, 1410
BstYI RGATCY 4 cut(s) 153, 220, 966, 1241
Bsu36I CCTNAGG 1 cut(s) 1026
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 4 cut(s) 132, 684, 953, 1390
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 3 cut(s) 133, 357, 1399
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 4 cut(s) 486, 888, 1062, 1318
Cac8I GCNNGC 3 cut(s) 238, 893, 917
Cfr13I GGNCC 4 cut(s) 591, 1022, 1252, 1388
Csp6I GTAC 2 cut(s) 258, 1195
CviAII CATG 7 cut(s) 92, 263, 340, 992, 1079, 1198, 1334
CviQI GTAC 2 cut(s) 258, 1195
DdeI CTNAG 4 cut(s) 150, 719, 1026, 1155
DpnI GATC 9 cut(s) 155, 222, 347, 552, 968, 1125, 1180, 1243, 1398
DpnII GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
DraI TTTAAA 1 cut(s) 1299
DraIII CACNNNGTG 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
Ecl136II GAGCTC 1 cut(s) 1154
Eco130I CCWWGG 2 cut(s) 231, 339
Eco24I GRGCYC 2 cut(s) 760, 1156
Eco47I GGWCC 3 cut(s) 591, 1022, 1252
Eco53kI GAGCTC 1 cut(s) 1154
Eco57I CTGAAG 1 cut(s) 61
Eco81I CCTNAGG 1 cut(s) 1026
Eco88I CYCGRG 1 cut(s) 672
EcoICRI GAGCTC 1 cut(s) 1154
EcoO109I RGGNCCY 1 cut(s) 1022
EcoRII CCWGG 3 cut(s) 1214, 1222, 1402
EcoT14I CCWWGG 2 cut(s) 231, 339
EcoT38I GRGCYC 2 cut(s) 760, 1156
ErhI CCWWGG 2 cut(s) 231, 339
FaeI CATG 7 cut(s) 95, 266, 343, 995, 1082, 1201, 1337
FaqI GGGAC 3 cut(s) 191, 1265, 1297
FatI CATG 7 cut(s) 91, 262, 339, 991, 1078, 1197, 1333
FauI CCCGC 1 cut(s) 1333
FblI GTMKAC 1 cut(s) 1137
Fnu4HI GCNGC 3 cut(s) 1220, 1228, 1478
FokI GGATG 3 cut(s) 140, 344, 1386
FriOI GRGCYC 2 cut(s) 760, 1156
Fsp4HI GCNGC 3 cut(s) 1220, 1228, 1478
FspBI CTAG 2 cut(s) 731, 1293
GluI GCNGC 3 cut(s) 1220, 1228, 1478
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 2 cut(s) 57, 582
HaeIII GGCC 4 cut(s) 132, 684, 953, 1390
HapII CCGG 4 cut(s) 447, 571, 589, 1326
Hin1I GRCGYC 1 cut(s) 1286
Hin1II CATG 7 cut(s) 95, 266, 343, 995, 1082, 1201, 1337
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 2 cut(s) 238, 1430
HinfI GANTC 4 cut(s) 443, 922, 986, 1316
HpaII CCGG 4 cut(s) 447, 571, 589, 1326
Hpy166II GTNNAC 3 cut(s) 336, 1138, 1331
Hpy188I TCNGA 5 cut(s) 378, 442, 533, 1178, 1375
Hpy188III TCNNGA 7 cut(s) 36, 349, 554, 599, 610, 747, 905
Hpy8I GTNNAC 3 cut(s) 336, 1138, 1331
HpyAV CCTTC 5 cut(s) 531, 638, 651, 902, 980
HpyCH4III ACNGT 4 cut(s) 461, 523, 586, 868
HpyCH4IV ACGT 2 cut(s) 306, 1286
HpyCH4V TGCA 4 cut(s) 68, 491, 817, 1230
HpyF10VI GCNNNNNNNGC 1 cut(s) 1004
HpyF3I CTNAG 4 cut(s) 150, 719, 1026, 1155
HpySE526I ACGT 2 cut(s) 306, 1286
Hsp92I GRCGYC 1 cut(s) 1286
Hsp92II CATG 7 cut(s) 95, 266, 343, 995, 1082, 1201, 1337
Kzo9I GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
LmnI GCTCC 5 cut(s) 661, 763, 1012, 1030, 1151
Lsp1109I GCAGC 3 cut(s) 1206, 1214, 1464
MaeI CTAG 2 cut(s) 731, 1293
MaeII ACGT 2 cut(s) 306, 1286
MaeIII GTNAC 8 cut(s) 180, 277, 517, 883, 1144, 1313, 1343, 1417
MalI GATC 9 cut(s) 155, 222, 347, 552, 968, 1125, 1180, 1243, 1398
MboI GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
MboII GAAGA 7 cut(s) 315, 524, 546, 725, 932, 950, 1251
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 4 cut(s) 153, 220, 966, 1241
MhlI GDGCHC 5 cut(s) 666, 760, 1009, 1088, 1156
MluCI AATT 9 cut(s) 61, 250, 300, 421, 651, 741, 818, 999, 1452
MlyI GAGTC 1 cut(s) 1310
MmeI TCCRAC 1 cut(s) 850
MnlI CCTC 9 cut(s) 32, 143, 372, 486, 536, 604, 714, 964, 1021
MroXI GAANNNNTTC 1 cut(s) 1070
MseI TTAA 5 cut(s) 15, 164, 309, 1298, 1460
MspA1I CMGCKG 1 cut(s) 81
MspCI CTTAAG 1 cut(s) 1459
MspI CCGG 4 cut(s) 447, 571, 589, 1326
MspR9I CCNGG 4 cut(s) 571, 1216, 1224, 1404
MunI CAATTG 1 cut(s) 999
MvaI CCWGG 3 cut(s) 1216, 1224, 1404
MwoI GCNNNNNNNGC 1 cut(s) 1004
NciI CCSGG 1 cut(s) 571
NcoI CCATGG 1 cut(s) 339
NdeII GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
NlaIII CATG 7 cut(s) 95, 266, 343, 995, 1082, 1201, 1337
NlaIV GGNNCC 5 cut(s) 222, 759, 798, 1253, 1389
NmuCI GTSAC 4 cut(s) 517, 883, 1144, 1313
NspI RCATGY 2 cut(s) 95, 1337
PaeR7I CTCGAG 1 cut(s) 672
PciI ACATGT 1 cut(s) 1333
PdmI GAANNNNTTC 1 cut(s) 1070
PfeI GAWTC 3 cut(s) 443, 922, 986
PkrI GCNGC 3 cut(s) 1221, 1229, 1479
PleI GAGTC 1 cut(s) 1310
PpsI GAGTC 1 cut(s) 1310
PpuMI RGGWCCY 1 cut(s) 1022
PscI ACATGT 1 cut(s) 1333
Psp124BI GAGCTC 1 cut(s) 1156
Psp5II RGGWCCY 1 cut(s) 1022
Psp6I CCWGG 3 cut(s) 1214, 1222, 1402
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 3 cut(s) 1214, 1222, 1402
PspN4I GGNNCC 5 cut(s) 222, 759, 798, 1253, 1389
PspPI GGNCC 4 cut(s) 591, 1022, 1252, 1388
PspPPI RGGWCCY 1 cut(s) 1022
PspXI VCTCGAGB 1 cut(s) 672
PsuI RGATCY 4 cut(s) 153, 220, 966, 1241
RsaI GTAC 2 cut(s) 259, 1196
RsaNI GTAC 2 cut(s) 258, 1195
SacI GAGCTC 1 cut(s) 1156
SaqAI TTAA 5 cut(s) 15, 164, 309, 1298, 1460
SatI GCNGC 3 cut(s) 1220, 1228, 1478
Sau3AI GATC 9 cut(s) 153, 220, 345, 550, 966, 1123, 1178, 1241, 1396
Sau96I GGNCC 4 cut(s) 591, 1022, 1252, 1388
SchI GAGTC 1 cut(s) 1310
ScrFI CCNGG 4 cut(s) 571, 1216, 1224, 1404
SduI GDGCHC 5 cut(s) 666, 760, 1009, 1088, 1156
Sfr274I CTCGAG 1 cut(s) 672
SinI GGWCC 3 cut(s) 591, 1022, 1252
SlaI CTCGAG 1 cut(s) 672
SmlI CTYRAG 3 cut(s) 672, 1318, 1459
SmoI CTYRAG 3 cut(s) 672, 1318, 1459
Sse9I AATT 9 cut(s) 61, 250, 300, 421, 651, 741, 818, 999, 1452
SsiI CCGC 3 cut(s) 81, 895, 1340
SspI AATATT 2 cut(s) 1361, 1472
SspMI CTAG 2 cut(s) 731, 1293
SstI GAGCTC 1 cut(s) 1156
StyD4I CCNGG 4 cut(s) 569, 1214, 1222, 1402
StyI CCWWGG 2 cut(s) 231, 339
TaaI ACNGT 4 cut(s) 461, 523, 586, 868
TaiI ACGT 2 cut(s) 309, 1289
TaqI TCGA 5 cut(s) 609, 673, 748, 906, 1050
TasI AATT 9 cut(s) 61, 250, 300, 421, 651, 741, 818, 999, 1452
TatI WGTACW 1 cut(s) 1194
TfiI GAWTC 3 cut(s) 443, 922, 986
Tru1I TTAA 5 cut(s) 15, 164, 309, 1298, 1460
Tru9I TTAA 5 cut(s) 15, 164, 309, 1298, 1460
TscAI CASTG 4 cut(s) 493, 888, 1062, 1318
TseFI GTSAC 4 cut(s) 517, 883, 1144, 1313
TseI GCWGC 3 cut(s) 1219, 1227, 1477
Tsp45I GTSAC 4 cut(s) 517, 883, 1144, 1313
TspDTI ATGAA 4 cut(s) 434, 1214, 1281, 1370
TspGWI ACGGA 1 cut(s) 889
TspRI CASTG 4 cut(s) 493, 888, 1062, 1318
Vha464I CTTAAG 1 cut(s) 1459
VpaK11BI GGWCC 3 cut(s) 591, 1022, 1252
XapI RAATTY 3 cut(s) 250, 421, 741
XceI RCATGY 2 cut(s) 95, 1337
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XhoI CTCGAG 1 cut(s) 672
XmiI GTMKAC 1 cut(s) 1137
XmnI GAANNNNTTC 1 cut(s) 1070
XspI CTAG 2 cut(s) 731, 1293
ZraI GACGTC 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.