RLG00000031906

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003665
Physical Location & Seq
Forward (+)
9388280 .. 9392960
4681 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031906

Sequence Viewer

Length: 1764 bp
ATGGCAACAAGAAGCTCGAGCTCAGAAGCTTTGACGACCACTCTGTCCAACTCAATCCAAGCTCTGGGTCGAGGCTTTGATGTGACTTCGGATATTAGGCTTCTGTATTGCAAAGGAGCACCTGGGTCAAGGCTTGTGAATCTGGATGAGAGTGGGGCTAAAGATCTTGCTGTTTCTGATGGGGTTGTGGTGCCAAATGTGCCTGCAGATATTGACTGCTCACCTGGGAAGAGAACCATTGAGAGAGTCCCTGTCTGTAGCTTCCATGAGATGGCAGAATACTTTAATAAGAAATCAGGTATATCAACCCGAATTCCCCTGGGAAGCTTCAATGCCATGTTTAATTTCACCGGTTCTTGGCAAGTTGATGCAGCAGCAACCAAATCCCTTGCTATGGTTGGATACTTTATTCCCCTATACAAAGTCAACTTAGAAACAGAAAATTTGGTTTTGCGTGATGAAATCAAGCGTGCTGTTCCTTACTCTTGGGATCCTGCAGCCTTGGCAAGTTTCATTGAGAACTTTGGTACACATATAGTCACATCTGCGACAATTGGTGGGAGAGATGTGGTCTATATCAGGCAGCACCAGTCATCTCCTTTATCATTATCGGACATTCAGAATTATGTGAAAGACATTGGTGATGATAGGTTTCTGGACTCAAAAGCCCAGTCAAGTGCTGGCCCCTTAAAATACAAGGACAAGGTTTCTTCCACTGACTCTCAAGTCTCATGGAACTTGAGAAAATCTCTCAAGTTCCAGTTGAAGAAAATGTCGCTTCTCCCAGTTGAAGTTACTGATGTTACGGTCATCTTCAGGAGAAGGGGAGGTGATGATCTTGAGCAGAGTCATTCCAAGTGGGCGGAGACGGTACAACTAGCACCAGATGTGATCAATATGACATTTACACCTATTGTTTCTCTTCTTGAAGGAGTGCCTGGAATAAAGCACTTGAGCCGTGCAATTGAGTTATATCTAGAGTACAAGCCTCCAATTGAAGATCTGCAGTATTTCTTGGACTTTCAAATTGCTCAGGTTTGGGCCCCAGAGCAAAATAATCTGCAAAGGAAAGAACCTGTCTGTTCATCCCTTCAATTCAGCTTGATGGGTCCCAAGCTTTATATCAGTCCGGATCAGGTAACAGTAGGCCGTAAGCCTGTCACTGGGCTCAGGCTTAACCTAGAAGGCAACAAGCAGAACCGACTTGCTATACATTTGCAGCATCTAGTGTCCCTTCCCAAGATTCTTCAACCCCATTGGGATGCCCATATGGCCATAGGTGCACCTAGGTGGCAAGGTCCAGAGGAGCAAGACAGTCGTTGGTTTGAGCCGATCAAGTGGAAGAACTTCTCCCACGTAAGCACTGCACCAATAGAGCATGTAGAGGCTTCTATTGGTGACCTCTCTGGTGTTCACATTGTCACAGGCGCTCAGCTTGGTGTTTGGGATTTTGGTGCCAAAAATGTTTTGCACCTCAAACTCCTCTTTTCAAAAGTACCAGGCTGCACAATCCGACGGTCTGTGTGGGATCACAGTCCCGCTGCCCCTTCTTCTGCACAGAGGCCTGATGGTTCTTCTTCATCACATTCAAATAGCAGAAACTCTGATGACAAAAAGGACAAACTAGCAAAGTTAGTAGACTTGACAGAAATGTCAAAGGGGCCGCAAGATATTCCTGGACATTGGTTAGTCACAGGAGCTAAGCTTGGAGTGGACAAGGGAAAGATTGTATTGCGTGTAAAATATTCCTTGCTAAACTACTGA

Protein Analysis

588

Amino Acids

65.01

Weight (kDa)

8.68

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 108 - 325 9.8e-33 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 43, 725, 1651
AccB1I GGYRCC 2 cut(s) 190, 1454
AccB7I CCANNNNNTGG 2 cut(s) 64, 271
AccI GTMKAC 1 cut(s) 1638
AccIII TCCGGA 1 cut(s) 1129
AciI CCGC 3 cut(s) 863, 1539, 1664
AclWI GGATC 4 cut(s) 485, 498, 1140, 1536
AcoI YGGCCR 1 cut(s) 1272
AcsI RAATTY 2 cut(s) 312, 442
AcuI CTGAAG 1 cut(s) 799
AfaI GTAC 4 cut(s) 529, 873, 983, 1497
AfiI CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1163
AgeI ACCGGT 1 cut(s) 350
AjnI CCWGG 6 cut(s) 121, 223, 318, 937, 1498, 1675
AleI CACNNNNGTG 1 cut(s) 1288
Alw21I GWGCWC 3 cut(s) 23, 121, 1285
Alw26I GTCTC 2 cut(s) 733, 860
Alw44I GTGCAC 1 cut(s) 1281
AlwI GGATC 4 cut(s) 485, 498, 1140, 1536
Ama87I CYCGRG 1 cut(s) 16
Aor13HI TCCGGA 1 cut(s) 1129
AoxI GGCC 6 cut(s) 682, 1041, 1147, 1272, 1562, 1661
ApaI GGGCCC 1 cut(s) 1045
ApaLI GTGCAC 1 cut(s) 1281
ApeKI GCWGC 7 cut(s) 371, 374, 497, 583, 1219, 1503, 1541
ApoI RAATTY 2 cut(s) 312, 442
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AsiGI ACCGGT 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 1346
AspA2I CCTAGG 1 cut(s) 1286
AspLEI GCGC 1 cut(s) 1430
AspS9I GGNCC 6 cut(s) 683, 1041, 1042, 1109, 1298, 1661
AsuHPI GGTGA 5 cut(s) 213, 340, 653, 842, 1409
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 2 cut(s) 1109, 1298
AvrII CCTAGG 1 cut(s) 1286
BaeGI GKGCMC 2 cut(s) 1045, 1285
BalI TGGCCA 1 cut(s) 1274
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 2 cut(s) 190, 1454
BanII GRGCYC 3 cut(s) 23, 1045, 1170
Bbv12I GWGCWC 3 cut(s) 23, 121, 1285
BbvI GCAGC 7 cut(s) 383, 386, 509, 595, 1231, 1490, 1528
BccI CCATC 4 cut(s) 173, 265, 1099, 1562
BceAI ACGGC 2 cut(s) 942, 1134
BcgI CGANNNNNNTGC 2 cut(s) 1298, 1332
BciT130I CCWGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
BciVI GTATCC 1 cut(s) 395
BclI TGATCA 1 cut(s) 891
BcoDI GTCTC 2 cut(s) 733, 860
BfaI CTAG 6 cut(s) 878, 977, 1181, 1226, 1287, 1625
BfmI CTRYAG 4 cut(s) 204, 256, 495, 1004
BfoI RGCGCY 1 cut(s) 1431
BfuI GTATCC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 1271
BglII AGATCT 2 cut(s) 163, 1000
BisI GCNGC 8 cut(s) 372, 375, 498, 584, 1220, 1504, 1542, 1664
BlnI CCTAGG 1 cut(s) 1286
BlpI GCTNAGC 2 cut(s) 1431, 1701
BlsI GCNGC 8 cut(s) 373, 376, 499, 585, 1221, 1505, 1543, 1665
Bme1390I CCNGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
Bme18I GGWCC 2 cut(s) 1109, 1298
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 6 cut(s) 683, 1041, 1042, 1109, 1298, 1661
BmiI GGNNCC 9 cut(s) 192, 492, 685, 1043, 1044, 1110, 1111, 1456, 1662
BmrFI CCNGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
BmrI ACTGGG 3 cut(s) 664, 779, 1173
BmsI GCATC 3 cut(s) 358, 1231, 1252
BmuI ACTGGG 3 cut(s) 664, 779, 1173
BplI GAGNNNNNCTC 2 cut(s) 733, 765
Bpu10I CCTNAGC 2 cut(s) 1032, 1169
Bpu1102I GCTNAGC 2 cut(s) 1431, 1701
BpuEI CTTGAG 5 cut(s) 708, 737, 760, 860, 973
BsaAI YACGTR 1 cut(s) 1357
BsaJI CCNNGG 6 cut(s) 122, 224, 318, 319, 501, 1286
BsaWI WCCGGW 2 cut(s) 350, 1129
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1163
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 5 cut(s) 589, 670, 760, 785, 1168
BseAI TCCGGA 1 cut(s) 1129
BseBI CCWGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
BseDI CCNNGG 6 cut(s) 122, 224, 318, 319, 501, 1286
BseGI GGATG 3 cut(s) 151, 1085, 1267
BseLI CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1163
BseMII CTCAG 4 cut(s) 36, 1046, 1183, 1445
BseNI ACTGG 5 cut(s) 589, 670, 760, 785, 1168
BseRI GAGGAG 2 cut(s) 1319, 1472
BseSI GKGCMC 2 cut(s) 1045, 1285
BseXI GCAGC 7 cut(s) 383, 386, 509, 595, 1231, 1490, 1528
BsgI GTGCAG 3 cut(s) 1350, 1489, 1539
BshFI GGCC 6 cut(s) 684, 1043, 1149, 1274, 1564, 1663
BshNI GGYRCC 2 cut(s) 190, 1454
BshTI ACCGGT 1 cut(s) 350
BsiHKAI GWGCWC 3 cut(s) 23, 121, 1285
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 2 cut(s) 351, 1130
BslFI GGGAC 4 cut(s) 233, 1095, 1216, 1521
BslI CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1163
BsmAI GTCTC 2 cut(s) 733, 860
BsmBI CGTCTC 1 cut(s) 860
BsmFI GGGAC 4 cut(s) 233, 1095, 1216, 1521
BsnI GGCC 6 cut(s) 684, 1043, 1149, 1274, 1564, 1663
BsoBI CYCGRG 1 cut(s) 16
Bsp120I GGGCCC 1 cut(s) 1041
Bsp1286I GDGCHC 5 cut(s) 23, 121, 1045, 1170, 1285
Bsp13I TCCGGA 1 cut(s) 1129
Bsp143I GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
Bsp1720I GCTNAGC 2 cut(s) 1431, 1701
BspACI CCGC 3 cut(s) 863, 1539, 1664
BspANI GGCC 6 cut(s) 684, 1043, 1149, 1274, 1564, 1663
BspCNI CTCAG 4 cut(s) 35, 1045, 1182, 1444
BspEI TCCGGA 1 cut(s) 1129
BspLI GGNNCC 9 cut(s) 192, 492, 685, 1043, 1044, 1110, 1111, 1456, 1662
BspMAI CTGCAG 3 cut(s) 208, 499, 1008
BspPI GGATC 4 cut(s) 485, 498, 1140, 1536
BspT107I GGYRCC 2 cut(s) 190, 1454
BsrFI RCCGGY 1 cut(s) 350
BsrI ACTGG 5 cut(s) 589, 670, 760, 785, 1168
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 6 cut(s) 122, 224, 318, 319, 501, 1286
BssMI GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
BssT1I CCWWGG 2 cut(s) 501, 1286
Bst2UI CCWGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
Bst4CI ACNGT 6 cut(s) 808, 871, 1144, 1316, 1518, 1535
Bst6I CTCTTC 2 cut(s) 224, 927
BstBAI YACGTR 1 cut(s) 1357
BstC8I GCNNGC 3 cut(s) 204, 471, 682
BstDEI CTNAG 6 cut(s) 22, 430, 1032, 1169, 1431, 1701
BstEII GGTNACC 1 cut(s) 1397
BstF5I GGATG 3 cut(s) 151, 1085, 1267
BstH2I RGCGCY 1 cut(s) 1431
BstHHI GCGC 1 cut(s) 1430
BstKTI GATC 8 cut(s) 166, 493, 838, 894, 1003, 1135, 1335, 1531
BstMAI GTCTC 2 cut(s) 733, 860
BstMBI GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
BstMWI GCNNNNNNNGC 4 cut(s) 199, 503, 1271, 1280
BstNI CCWGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
BstNSI RCATGY 1 cut(s) 1382
BstPI GGTNACC 1 cut(s) 1397
BstSCI CCNGG 6 cut(s) 121, 223, 318, 937, 1498, 1675
BstSFI CTRYAG 4 cut(s) 204, 256, 495, 1004
BstSLI GKGCMC 2 cut(s) 1045, 1285
BstV1I GCAGC 7 cut(s) 383, 386, 509, 595, 1231, 1490, 1528
BstX2I RGATCY 3 cut(s) 163, 490, 1000
BstYI RGATCY 3 cut(s) 163, 490, 1000
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 6 cut(s) 684, 1043, 1149, 1274, 1564, 1663
BtsCI GGATG 3 cut(s) 151, 1085, 1267
BtsI GCAGTG 1 cut(s) 1362
BtsIMutI CAGTG 3 cut(s) 714, 1161, 1362
Cac8I GCNNGC 3 cut(s) 204, 471, 682
CfoI GCGC 1 cut(s) 1430
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 6 cut(s) 683, 1041, 1042, 1109, 1298, 1661
Csp6I GTAC 4 cut(s) 528, 872, 982, 1496
CspAI ACCGGT 1 cut(s) 350
CviAII CATG 4 cut(s) 266, 337, 732, 1379
CviQI GTAC 4 cut(s) 528, 872, 982, 1496
DdeI CTNAG 6 cut(s) 22, 430, 1032, 1169, 1431, 1701
DpnI GATC 8 cut(s) 165, 492, 837, 893, 1002, 1134, 1334, 1530
DpnII GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
DrdI GACNNNNNNGTC 3 cut(s) 43, 725, 1651
DseDI GACNNNNNNGTC 3 cut(s) 43, 725, 1651
EaeI YGGCCR 1 cut(s) 1272
Eam1104I CTCTTC 2 cut(s) 224, 927
EarI CTCTTC 2 cut(s) 224, 927
EciI GGCGGA 1 cut(s) 878
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 501, 1286
Eco147I AGGCCT 1 cut(s) 1564
Eco24I GRGCYC 3 cut(s) 23, 1045, 1170
Eco47I GGWCC 2 cut(s) 1109, 1298
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 799
Eco88I CYCGRG 1 cut(s) 16
Eco91I GGTNACC 1 cut(s) 1397
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 2 cut(s) 1042, 1109
EcoO65I GGTNACC 1 cut(s) 1397
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 6 cut(s) 121, 223, 318, 937, 1498, 1675
EcoT14I CCWWGG 2 cut(s) 501, 1286
EcoT38I GRGCYC 3 cut(s) 23, 1045, 1170
ErhI CCWWGG 2 cut(s) 501, 1286
Esp3I CGTCTC 1 cut(s) 860
FaeI CATG 4 cut(s) 269, 340, 735, 1382
FaqI GGGAC 4 cut(s) 233, 1095, 1216, 1521
FatI CATG 4 cut(s) 265, 336, 731, 1378
FauI CCCGC 1 cut(s) 1546
FauNDI CATATG 1 cut(s) 1269
FbaI TGATCA 1 cut(s) 891
FblI GTMKAC 1 cut(s) 1638
Fnu4HI GCNGC 8 cut(s) 372, 375, 498, 584, 1220, 1504, 1542, 1664
FokI GGATG 3 cut(s) 158, 1072, 1274
FriOI GRGCYC 3 cut(s) 23, 1045, 1170
Fsp4HI GCNGC 8 cut(s) 372, 375, 498, 584, 1220, 1504, 1542, 1664
FspBI CTAG 6 cut(s) 878, 977, 1181, 1226, 1287, 1625
GlaI GCGC 1 cut(s) 1429
GluI GCNGC 8 cut(s) 372, 375, 498, 584, 1220, 1504, 1542, 1664
HaeII RGCGCY 1 cut(s) 1431
HaeIII GGCC 6 cut(s) 684, 1043, 1149, 1274, 1564, 1663
HapII CCGG 2 cut(s) 351, 1130
HhaI GCGC 1 cut(s) 1430
Hin1II CATG 4 cut(s) 269, 340, 735, 1382
Hin6I GCGC 1 cut(s) 1428
HinP1I GCGC 1 cut(s) 1428
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 4 cut(s) 27, 325, 1115, 1703
HinfI GANTC 6 cut(s) 139, 246, 659, 719, 847, 1243
HpaII CCGG 2 cut(s) 351, 1130
HphI GGTGA 5 cut(s) 213, 340, 653, 842, 1409
Hpy166II GTNNAC 6 cut(s) 427, 530, 1283, 1414, 1639, 1714
Hpy188I TCNGA 7 cut(s) 25, 91, 178, 613, 621, 1514, 1606
Hpy188III TCNNGA 8 cut(s) 143, 656, 817, 839, 926, 977, 1130, 1301
Hpy8I GTNNAC 6 cut(s) 427, 530, 1283, 1414, 1639, 1714
Hpy99I CGWCG 1 cut(s) 1518
HpyAV CCTTC 6 cut(s) 816, 923, 1100, 1178, 1244, 1557
HpyCH4III ACNGT 6 cut(s) 808, 871, 1144, 1316, 1518, 1535
HpyCH4IV ACGT 1 cut(s) 1356
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 503, 1271, 1280
HpyF3I CTNAG 6 cut(s) 22, 430, 1032, 1169, 1431, 1701
HpySE526I ACGT 1 cut(s) 1356
Hsp92II CATG 4 cut(s) 269, 340, 735, 1382
HspAI GCGC 1 cut(s) 1428
KflI GGGWCCC 1 cut(s) 1109
Kpn2I TCCGGA 1 cut(s) 1129
Ksp22I TGATCA 1 cut(s) 891
Kzo9I GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
LmnI GCTCC 3 cut(s) 116, 1306, 1697
Lsp1109I GCAGC 7 cut(s) 383, 386, 509, 595, 1231, 1490, 1528
LweI GCATC 3 cut(s) 358, 1231, 1252
MaeI CTAG 6 cut(s) 878, 977, 1181, 1226, 1287, 1625
MaeII ACGT 1 cut(s) 1356
MaeIII GTNAC 9 cut(s) 82, 538, 793, 802, 1138, 1159, 1397, 1420, 1690
MalI GATC 8 cut(s) 165, 492, 837, 893, 1002, 1134, 1334, 1530
MboI GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
MfeI CAATTG 3 cut(s) 552, 963, 993
MflI RGATCY 3 cut(s) 163, 490, 1000
MhlI GDGCHC 5 cut(s) 23, 121, 1045, 1170, 1285
MlsI TGGCCA 1 cut(s) 1274
MluCI AATT 9 cut(s) 312, 343, 442, 552, 622, 963, 993, 1026, 1094
MluNI TGGCCA 1 cut(s) 1274
MlyI GAGTC 4 cut(s) 255, 653, 713, 856
MmeI TCCRAC 3 cut(s) 72, 379, 1537
MnlI CCTC 9 cut(s) 65, 821, 999, 1297, 1378, 1412, 1484, 1493, 1554
Mox20I TGGCCA 1 cut(s) 1274
MroI TCCGGA 1 cut(s) 1129
MroXI GAANNNNTTC 1 cut(s) 1346
MscI TGGCCA 1 cut(s) 1274
MseI TTAA 4 cut(s) 285, 342, 689, 1176
MslI CAYNNNNRTG 2 cut(s) 1260, 1288
Msp20I TGGCCA 1 cut(s) 1274
MspA1I CMGCKG 1 cut(s) 1541
MspI CCGG 2 cut(s) 351, 1130
MspR9I CCNGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
MunI CAATTG 3 cut(s) 552, 963, 993
MvaI CCWGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
MwoI GCNNNNNNNGC 4 cut(s) 199, 503, 1271, 1280
NdeI CATATG 1 cut(s) 1269
NdeII GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
NlaIII CATG 4 cut(s) 269, 340, 735, 1382
NlaIV GGNNCC 9 cut(s) 192, 492, 685, 1043, 1044, 1110, 1111, 1456, 1662
NmuCI GTSAC 6 cut(s) 82, 538, 1159, 1397, 1420, 1690
NspI RCATGY 1 cut(s) 1382
OliI CACNNNNGTG 1 cut(s) 1288
PaeR7I CTCGAG 1 cut(s) 16
PasI CCCWGGG 1 cut(s) 319
PceI AGGCCT 1 cut(s) 1564
PdmI GAANNNNTTC 1 cut(s) 1346
PfeI GAWTC 2 cut(s) 139, 1243
PflMI CCANNNNNTGG 2 cut(s) 64, 271
PfoI TCCNGGA 1 cut(s) 1675
PinAI ACCGGT 1 cut(s) 350
PkrI GCNGC 8 cut(s) 373, 376, 499, 585, 1221, 1505, 1543, 1665
PleI GAGTC 4 cut(s) 254, 653, 713, 855
PpsI GAGTC 4 cut(s) 254, 653, 713, 855
Ppu21I YACGTR 1 cut(s) 1357
PpuMI RGGWCCY 1 cut(s) 1109
Psp124BI GAGCTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 1109
Psp6I CCWGG 6 cut(s) 121, 223, 318, 937, 1498, 1675
PspEI GGTNACC 1 cut(s) 1397
PspGI CCWGG 6 cut(s) 121, 223, 318, 937, 1498, 1675
PspN4I GGNNCC 9 cut(s) 192, 492, 685, 1043, 1044, 1110, 1111, 1456, 1662
PspOMI GGGCCC 1 cut(s) 1041
PspPI GGNCC 6 cut(s) 683, 1041, 1042, 1109, 1298, 1661
PspPPI RGGWCCY 1 cut(s) 1109
PspXI VCTCGAGB 1 cut(s) 16
PstI CTGCAG 3 cut(s) 208, 499, 1008
PsuI RGATCY 3 cut(s) 163, 490, 1000
RsaI GTAC 4 cut(s) 529, 873, 983, 1497
RsaNI GTAC 4 cut(s) 528, 872, 982, 1496
RseI CAYNNNNRTG 2 cut(s) 1260, 1288
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 4 cut(s) 285, 342, 689, 1176
SatI GCNGC 8 cut(s) 372, 375, 498, 584, 1220, 1504, 1542, 1664
Sau3AI GATC 8 cut(s) 163, 490, 835, 891, 1000, 1132, 1332, 1528
Sau96I GGNCC 6 cut(s) 683, 1041, 1042, 1109, 1298, 1661
SchI GAGTC 4 cut(s) 255, 653, 713, 856
ScrFI CCNGG 6 cut(s) 123, 225, 320, 939, 1500, 1677
SduI GDGCHC 5 cut(s) 23, 121, 1045, 1170, 1285
SfaNI GCATC 3 cut(s) 358, 1231, 1252
SfcI CTRYAG 4 cut(s) 204, 256, 495, 1004
Sfr274I CTCGAG 1 cut(s) 16
SinI GGWCC 2 cut(s) 1109, 1298
SlaI CTCGAG 1 cut(s) 16
SmiMI CAYNNNNRTG 2 cut(s) 1260, 1288
SmlI CTYRAG 6 cut(s) 16, 723, 739, 752, 839, 952
SmoI CTYRAG 6 cut(s) 16, 723, 739, 752, 839, 952
Sse9I AATT 9 cut(s) 312, 343, 442, 552, 622, 963, 993, 1026, 1094
SseBI AGGCCT 1 cut(s) 1564
SsiI CCGC 3 cut(s) 863, 1539, 1664
SspI AATATT 1 cut(s) 1745
SspMI CTAG 6 cut(s) 878, 977, 1181, 1226, 1287, 1625
SstI GAGCTC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 1564
StyD4I CCNGG 6 cut(s) 121, 223, 318, 937, 1498, 1675
StyI CCWWGG 2 cut(s) 501, 1286
TaaI ACNGT 6 cut(s) 808, 871, 1144, 1316, 1518, 1535
TaiI ACGT 1 cut(s) 1359
TaqI TCGA 2 cut(s) 17, 70
TasI AATT 9 cut(s) 312, 343, 442, 552, 622, 963, 993, 1026, 1094
TatI WGTACW 1 cut(s) 981
TauI GCSGC 1 cut(s) 1666
TfiI GAWTC 2 cut(s) 139, 1243
Tru1I TTAA 4 cut(s) 285, 342, 689, 1176
Tru9I TTAA 4 cut(s) 285, 342, 689, 1176
TscAI CASTG 3 cut(s) 721, 1168, 1369
TseFI GTSAC 6 cut(s) 82, 538, 1159, 1397, 1420, 1690
TseI GCWGC 7 cut(s) 371, 374, 497, 583, 1219, 1503, 1541
Tsp45I GTSAC 6 cut(s) 82, 538, 1159, 1397, 1420, 1690
TspDTI ATGAA 4 cut(s) 474, 502, 1074, 1569
TspRI CASTG 3 cut(s) 721, 1168, 1369
Van91I CCANNNNNTGG 2 cut(s) 64, 271
VneI GTGCAC 1 cut(s) 1281
VpaK11BI GGWCC 2 cut(s) 1109, 1298
XapI RAATTY 2 cut(s) 312, 442
XbaI TCTAGA 1 cut(s) 976
XceI RCATGY 1 cut(s) 1382
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XhoI CTCGAG 1 cut(s) 16
XmaJI CCTAGG 1 cut(s) 1286
XmiI GTMKAC 1 cut(s) 1638
XmnI GAANNNNTTC 1 cut(s) 1346
XspI CTAG 6 cut(s) 878, 977, 1181, 1226, 1287, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.