Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0003665

Physical Location & Seq

Genomic Coordinates Reverse (-)

25050108 .. 25051396

Gene Structure 1289 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g22730.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGATCGATCCTCTTGAGCAGAGTCATCCCAAGTGGGCCGAAACTGTACAACTAGCACCAGATGTGATTAACATGACATTTACACCCATTGTCTCTTTGCTTGAAGGAGTGCCTGGAATAAAGCACTTGAGTCGCGCGATTGAGTTATATTTAGAGTACAAGCCACCAATTGAAGATCTGCAGTATTTCTTGGATTTTCAAATTGCTCAAGTTTGGGCCCCGGAGCATAATAATCTTCAAAGAAAAGAACCTGTGTGTACATCACTTCAGTTCAGCTTGATGGGACCGAAGCTTTACATCAGTCCGGATCAGGTAACAGTAGGCCGTAAGCCTGTCACAGGGCTTAGACTAAATCTAGAAGGCAGCAAACAGAACCGACTGGCTATACATTTGCAACATCTAGTGTCCCTTCCAAAAATTCTTCAACCCCATTGGGATGCGCACATGGCAATAGGTGCACCCAAGTGGCAAGGTCCGGAGGAGCAAGACAGCCGTTGGTTTGAACCAATCAAGTGGAAAAACTTCTCCCATGTAAGCACTGCACCGATAGAGCACGTGGAGGCTTCTATTGGTGACCTCTCCGGTGTTCACATTGTCACGGGGGCTCAGCTTGGTGTTTGGGATTTTGGTGCCAAAAATGTATTGCACCTCAAACTCCTCTTCTCCAAAGTACCAGGCTGCACTATCAGGCGTTCTGTTTGGGATCACAGCCCAGCCAGCCTGTCTTCTGCACAGAGGACGGATGGTTCTTCTTCATCGCATTTGAACAACAGAAGCTCTGACGATAAAAAGGACAAACTAGCAAAACTAGTGGACTTGACAGAAATGTCAAAGGGACCGCAAGATATACCAGGTCATTGGTTAGTCACCGGGGCTAAGCTTGGAGTCGACAAGGGAAAGATTATATTGCGCGTAAAATATTCCTTGCTGAACTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0010337 GO:0010565 GO:0010817 GO:0012501 GO:0019222 GO:0031323 GO:0032350 GO:0033554 GO:0034050 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0062012 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MACPF

Protein Analysis

313

Amino Acids

34.95

Weight (kDa)

8.39

Isoelectric Point (pI)

45.82

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003665)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29690
fragaria_vesca	FvH4_6g10020 FvH4_6g10020 FvH4_6g10020 FvH4_7g07810 FvH4_7g07810 FvH4_7g07810
malus_domestica	MD04G1173300.v1.1 MD05G1294000.v1.1 MD12G1186700.v1.1
prunus_persica	Prupe.4G070000_v2.0.a1 Prupe.6G292200_v2.0.a1
pyrus_communis	pycom05g27130 pycom10g22730 pycom12g17520
rosa_chinensis	RchiOBHm_Chr3g0460701 RchiOBHm_Chr5g0012351
rosa_laevigata	RLG00000024987 RLG00000031906
rosa_multiflora	Rmu_co8488383.1_g000001 Rmu_sc0011069.1_g000008
rosa_roxburghii	Rroxscaffold_1G00064310 Rroxscaffold_6G00419290
rosa_rugosa	Rorug03G0045300 Rorug05G0000700
rosa_samantha	Rh3AG102800 Rh3BG106500 Rh3CG108400 Rh3DG108000 Rh5AG094800 Rh5BG091000 Rh5CG104100 Rh5DG090500
rosa_wichuraiana	Rw3G008750 Rw5G008280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	826
Acc16I	TGCGCA	1 cut(s)	441
AccB1I	GGYRCC	1 cut(s)	629
AccI	GTMKAC	1 cut(s)	888
AccII	CGCG	3 cut(s)	135, 137, 912
AccIII	TCCGGA	2 cut(s)	304, 475
AciI	CCGC	1 cut(s)	839
AclWI	GGATC	3 cut(s)	2, 315, 711
AcsI	RAATTY	1 cut(s)	417
AcuI	CTGAAG	1 cut(s)	251
AcvI	CACGTG	1 cut(s)	556
AfaI	GTAC	4 cut(s)	48, 158, 259, 672
AfiI	CCNNNNNNNGG	1 cut(s)	338
AgsI	TTSAA	7 cut(s)	104, 173, 200, 239, 425, 503, 766
AhlI	ACTAGT	1 cut(s)	808
AjnI	CCWGG	3 cut(s)	112, 673, 850
AleI	CACNNNNGTG	1 cut(s)	463
AloI	GAACNNNNNNTCC	2 cut(s)	730, 762
AluBI	AGCT	5 cut(s)	276, 292, 610, 777, 880
AluI	AGCT	5 cut(s)	276, 292, 610, 777, 880
Alw21I	GWGCWC	2 cut(s)	460, 555
Alw26I	GTCTC	1 cut(s)	97
Alw44I	GTGCAC	1 cut(s)	456
AlwI	GGATC	3 cut(s)	2, 315, 711
Aor13HI	TCCGGA	2 cut(s)	304, 475
AoxI	GGCC	3 cut(s)	36, 216, 322
ApaI	GGGCCC	1 cut(s)	220
ApaLI	GTGCAC	1 cut(s)	456
ApeKI	GCWGC	2 cut(s)	363, 678
ApoI	RAATTY	1 cut(s)	417
Asp700I	GAANNNNTTC	1 cut(s)	521
AspLEI	GCGC	3 cut(s)	137, 442, 912
AspS9I	GGNCC	6 cut(s)	36, 216, 217, 284, 473, 836
AsuC2I	CCSGG	2 cut(s)	221, 871
AsuHPI	GGTGA	2 cut(s)	584, 859
AvaII	GGWCC	3 cut(s)	284, 473, 836
BaeGI	GKGCMC	2 cut(s)	220, 460
BanI	GGYRCC	1 cut(s)	629
BanII	GRGCYC	2 cut(s)	220, 607
BbrPI	CACGTG	1 cut(s)	556
BbsI	GAAGAC	1 cut(s)	717
Bbv12I	GWGCWC	2 cut(s)	460, 555
BbvI	GCAGC	2 cut(s)	375, 665
BccI	CCATC	2 cut(s)	274, 737
BceAI	ACGGC	2 cut(s)	309, 477
BcgI	CGANNNNNNTGC	2 cut(s)	113, 147
BciT130I	CCWGG	3 cut(s)	114, 675, 852
BcnI	CCSGG	2 cut(s)	221, 871
BcoDI	GTCTC	1 cut(s)	97
BcuI	ACTAGT	1 cut(s)	808
BfaI	CTAG	5 cut(s)	53, 356, 401, 800, 809
BfmI	CTRYAG	1 cut(s)	179
BglII	AGATCT	1 cut(s)	175
BisI	GCNGC	2 cut(s)	364, 679
BlpI	GCTNAGC	2 cut(s)	606, 876
BlsI	GCNGC	2 cut(s)	365, 680
Bme1390I	CCNGG	5 cut(s)	114, 221, 675, 852, 871
Bme18I	GGWCC	3 cut(s)	284, 473, 836
BmgT120I	GGNCC	6 cut(s)	36, 216, 217, 284, 473, 836
BmiI	GGNNCC	5 cut(s)	218, 219, 285, 631, 837
BmrFI	CCNGG	5 cut(s)	114, 221, 675, 852, 871
BmsI	GCATC	1 cut(s)	427
BpiI	GAAGAC	1 cut(s)	717
Bpu1102I	GCTNAGC	2 cut(s)	606, 876
BpuEI	CTTGAG	3 cut(s)	35, 148, 192
BpuMI	CCSGG	2 cut(s)	221, 871
Bsa29I	ATCGAT	1 cut(s)	6
BsaAI	YACGTR	1 cut(s)	556
BsaJI	CCNNGG	2 cut(s)	219, 870
BsaWI	WCCGGW	3 cut(s)	304, 475, 581
Bsc4I	CCNNNNNNNGG	1 cut(s)	338
Bse1I	ACTGG	1 cut(s)	384
BseAI	TCCGGA	2 cut(s)	304, 475
BseBI	CCWGG	3 cut(s)	114, 675, 852
BseCI	ATCGAT	1 cut(s)	6
BseDI	CCNNGG	2 cut(s)	219, 870
BseGI	GGATG	3 cut(s)	25, 442, 748
BseLI	CCNNNNNNNGG	1 cut(s)	338
BseMII	CTCAG	1 cut(s)	620
BseNI	ACTGG	1 cut(s)	384
BseRI	GAGGAG	2 cut(s)	494, 647
BseSI	GKGCMC	2 cut(s)	220, 460
BseXI	GCAGC	2 cut(s)	375, 665
BseYI	CCCAGC	1 cut(s)	712
BsgI	GTGCAG	3 cut(s)	525, 664, 714
Bsh1236I	CGCG	3 cut(s)	135, 137, 912
BshFI	GGCC	3 cut(s)	38, 218, 324
BshNI	GGYRCC	1 cut(s)	629
BshVI	ATCGAT	1 cut(s)	6
BsiHKAI	GWGCWC	2 cut(s)	460, 555
BsiSI	CCGG	5 cut(s)	221, 305, 476, 582, 870
BslFI	GGGAC	3 cut(s)	297, 391, 849
BslI	CCNNNNNNNGG	1 cut(s)	338
BsmAI	GTCTC	1 cut(s)	97
BsmFI	GGGAC	3 cut(s)	297, 391, 849
BsnI	GGCC	3 cut(s)	38, 218, 324
Bsp120I	GGGCCC	1 cut(s)	216
Bsp1286I	GDGCHC	4 cut(s)	220, 460, 555, 607
Bsp13I	TCCGGA	2 cut(s)	304, 475
Bsp1407I	TGTACA	2 cut(s)	46, 257
Bsp143I	GATC	5 cut(s)	3, 7, 175, 307, 703
Bsp1720I	GCTNAGC	2 cut(s)	606, 876
BspACI	CCGC	1 cut(s)	839
BspANI	GGCC	3 cut(s)	38, 218, 324
BspCNI	CTCAG	1 cut(s)	619
BspDI	ATCGAT	1 cut(s)	6
BspEI	TCCGGA	2 cut(s)	304, 475
BspFNI	CGCG	3 cut(s)	135, 137, 912
BspLI	GGNNCC	5 cut(s)	218, 219, 285, 631, 837
BspMAI	CTGCAG	1 cut(s)	183
BspPI	GGATC	3 cut(s)	2, 315, 711
BspT107I	GGYRCC	1 cut(s)	629
BsrGI	TGTACA	2 cut(s)	46, 257
BsrI	ACTGG	1 cut(s)	384
BssECI	CCNNGG	2 cut(s)	219, 870
BssMI	GATC	5 cut(s)	3, 7, 175, 307, 703
Bst2UI	CCWGG	3 cut(s)	114, 675, 852
Bst4CI	ACNGT	2 cut(s)	46, 319
Bst6I	CTCTTC	1 cut(s)	665
BstAPI	GCANNNNNTGC	1 cut(s)	455
BstAUI	TGTACA	2 cut(s)	46, 257
BstBAI	YACGTR	1 cut(s)	556
BstC8I	GCNNGC	1 cut(s)	718
BstDEI	CTNAG	3 cut(s)	344, 606, 876
BstEII	GGTNACC	1 cut(s)	572
BstENI	CCTNNNNNAGG	1 cut(s)	336
BstF5I	GGATG	3 cut(s)	25, 442, 748
BstFNI	CGCG	3 cut(s)	135, 137, 912
BstHHI	GCGC	3 cut(s)	137, 442, 912
BstKTI	GATC	5 cut(s)	6, 10, 178, 310, 706
BstMAI	GTCTC	1 cut(s)	97
BstMBI	GATC	5 cut(s)	3, 7, 175, 307, 703
BstMWI	GCNNNNNNNGC	3 cut(s)	446, 455, 717
BstNI	CCWGG	3 cut(s)	114, 675, 852
BstPI	GGTNACC	1 cut(s)	572
BstSCI	CCNGG	5 cut(s)	112, 219, 673, 850, 869
BstSFI	CTRYAG	1 cut(s)	179
BstSLI	GKGCMC	2 cut(s)	220, 460
BstUI	CGCG	3 cut(s)	135, 137, 912
BstV1I	GCAGC	2 cut(s)	375, 665
BstV2I	GAAGAC	1 cut(s)	717
BstX2I	RGATCY	1 cut(s)	175
BstXI	CCANNNNNNTGG	2 cut(s)	513, 858
BstYI	RGATCY	1 cut(s)	175
Bsu15I	ATCGAT	1 cut(s)	6
BsuRI	GGCC	3 cut(s)	38, 218, 324
BsuTUI	ATCGAT	1 cut(s)	6
BtgZI	GCGATG	1 cut(s)	741
BtsCI	GGATG	3 cut(s)	25, 442, 748
BtsI	GCAGTG	1 cut(s)	537
BtsIMutI	CAGTG	1 cut(s)	537
Cac8I	GCNNGC	1 cut(s)	718
CfoI	GCGC	3 cut(s)	137, 442, 912
Cfr13I	GGNCC	6 cut(s)	36, 216, 217, 284, 473, 836
ClaI	ATCGAT	1 cut(s)	6
CsiI	ACCWGGT	1 cut(s)	850
Csp6I	GTAC	4 cut(s)	47, 157, 258, 671
CspCI	CAANNNNNGTGG	2 cut(s)	792, 827
CviAII	CATG	3 cut(s)	73, 445, 530
CviQI	GTAC	4 cut(s)	47, 157, 258, 671
DdeI	CTNAG	3 cut(s)	344, 606, 876
DpnI	GATC	5 cut(s)	5, 9, 177, 309, 705
DpnII	GATC	5 cut(s)	3, 7, 175, 307, 703
DrdI	GACNNNNNNGTC	1 cut(s)	826
DseDI	GACNNNNNNGTC	1 cut(s)	826
Eam1104I	CTCTTC	1 cut(s)	665
EarI	CTCTTC	1 cut(s)	665
Eco24I	GRGCYC	2 cut(s)	220, 607
Eco47I	GGWCC	3 cut(s)	284, 473, 836
Eco57I	CTGAAG	1 cut(s)	251
Eco72I	CACGTG	1 cut(s)	556
Eco91I	GGTNACC	1 cut(s)	572
EcoNI	CCTNNNNNAGG	1 cut(s)	336
EcoO109I	RGGNCCY	1 cut(s)	217
EcoO65I	GGTNACC	1 cut(s)	572
EcoRII	CCWGG	3 cut(s)	112, 673, 850
EcoT38I	GRGCYC	2 cut(s)	220, 607
FaeI	CATG	3 cut(s)	76, 448, 533
FaiI	YATR	8 cut(s)	74, 148, 228, 386, 446, 531, 848, 905
FaqI	GGGAC	3 cut(s)	297, 391, 849
FatI	CATG	3 cut(s)	72, 444, 529
FblI	GTMKAC	1 cut(s)	888
Fnu4HI	GCNGC	2 cut(s)	364, 679
FokI	GGATG	3 cut(s)	12, 449, 755
FriOI	GRGCYC	2 cut(s)	220, 607
Fsp4HI	GCNGC	2 cut(s)	364, 679
FspAI	RTGCGCAY	1 cut(s)	441
FspBI	CTAG	5 cut(s)	53, 356, 401, 800, 809
FspI	TGCGCA	1 cut(s)	441
GlaI	GCGC	3 cut(s)	136, 441, 911
GluI	GCNGC	2 cut(s)	364, 679
GsaI	CCCAGC	1 cut(s)	716
HaeIII	GGCC	3 cut(s)	38, 218, 324
HapII	CCGG	5 cut(s)	221, 305, 476, 582, 870
HhaI	GCGC	3 cut(s)	137, 442, 912
Hin1II	CATG	3 cut(s)	76, 448, 533
Hin6I	GCGC	3 cut(s)	135, 440, 910
HinP1I	GCGC	3 cut(s)	135, 440, 910
HincII	GTYRAC	1 cut(s)	889
HindII	GTYRAC	1 cut(s)	889
HindIII	AAGCTT	2 cut(s)	290, 878
HinfI	GANTC	3 cut(s)	22, 130, 885
HpaII	CCGG	5 cut(s)	221, 305, 476, 582, 870
HphI	GGTGA	2 cut(s)	584, 859
Hpy166II	GTNNAC	5 cut(s)	258, 458, 589, 814, 889
Hpy188I	TCNGA	1 cut(s)	781
Hpy188III	TCNNGA	4 cut(s)	14, 305, 356, 476
Hpy8I	GTNNAC	5 cut(s)	258, 458, 589, 814, 889
HpyAV	CCTTC	3 cut(s)	98, 353, 419
HpyCH4III	ACNGT	2 cut(s)	46, 319
HpyCH4IV	ACGT	1 cut(s)	555
HpyCH4V	TGCA	7 cut(s)	181, 394, 458, 542, 646, 681, 731
HpyF10VI	GCNNNNNNNGC	3 cut(s)	446, 455, 717
HpyF3I	CTNAG	3 cut(s)	344, 606, 876
HpySE526I	ACGT	1 cut(s)	555
Hsp92II	CATG	3 cut(s)	76, 448, 533
HspAI	GCGC	3 cut(s)	135, 440, 910
Kpn2I	TCCGGA	2 cut(s)	304, 475
Kzo9I	GATC	5 cut(s)	3, 7, 175, 307, 703
LmnI	GCTCC	2 cut(s)	223, 481
Lsp1109I	GCAGC	2 cut(s)	375, 665
LweI	GCATC	1 cut(s)	427
MabI	ACCWGGT	1 cut(s)	850
MaeI	CTAG	5 cut(s)	53, 356, 401, 800, 809
MaeII	ACGT	1 cut(s)	555
MaeIII	GTNAC	5 cut(s)	313, 334, 572, 595, 865
MalI	GATC	5 cut(s)	5, 9, 177, 309, 705
MboI	GATC	5 cut(s)	3, 7, 175, 307, 703
MboII	GAAGA	7 cut(s)	185, 227, 413, 652, 717, 741, 744
MfeI	CAATTG	1 cut(s)	168
MflI	RGATCY	1 cut(s)	175
MhlI	GDGCHC	4 cut(s)	220, 460, 555, 607
MluCI	AATT	3 cut(s)	168, 201, 417
MlyI	GAGTC	3 cut(s)	31, 139, 894
MnlI	CCTC	7 cut(s)	21, 472, 553, 587, 659, 668, 729
MroI	TCCGGA	2 cut(s)	304, 475
MroXI	GAANNNNTTC	1 cut(s)	521
MseI	TTAA	1 cut(s)	69
MslI	CAYNNNNRTG	2 cut(s)	435, 463
MspI	CCGG	5 cut(s)	221, 305, 476, 582, 870
MspR9I	CCNGG	5 cut(s)	114, 221, 675, 852, 871
MunI	CAATTG	1 cut(s)	168
MvaI	CCWGG	3 cut(s)	114, 675, 852
MvnI	CGCG	3 cut(s)	135, 137, 912
MwoI	GCNNNNNNNGC	3 cut(s)	446, 455, 717
NciI	CCSGG	2 cut(s)	221, 871
NdeII	GATC	5 cut(s)	3, 7, 175, 307, 703
NlaIII	CATG	3 cut(s)	76, 448, 533
NlaIV	GGNNCC	5 cut(s)	218, 219, 285, 631, 837
NmuCI	GTSAC	4 cut(s)	334, 572, 595, 865
NsbI	TGCGCA	1 cut(s)	441
OliI	CACNNNNGTG	1 cut(s)	463
PdmI	GAANNNNTTC	1 cut(s)	521
PkrI	GCNGC	2 cut(s)	365, 680
PleI	GAGTC	3 cut(s)	30, 138, 893
PmaCI	CACGTG	1 cut(s)	556
PmlI	CACGTG	1 cut(s)	556
PpsI	GAGTC	3 cut(s)	30, 138, 893
Ppu21I	YACGTR	1 cut(s)	556
Psp6I	CCWGG	3 cut(s)	112, 673, 850
PspCI	CACGTG	1 cut(s)	556
PspEI	GGTNACC	1 cut(s)	572
PspFI	CCCAGC	1 cut(s)	712
PspGI	CCWGG	3 cut(s)	112, 673, 850
PspN4I	GGNNCC	5 cut(s)	218, 219, 285, 631, 837
PspOMI	GGGCCC	1 cut(s)	216
PspPI	GGNCC	6 cut(s)	36, 216, 217, 284, 473, 836
PstI	CTGCAG	1 cut(s)	183
PsuI	RGATCY	1 cut(s)	175
RsaI	GTAC	4 cut(s)	48, 158, 259, 672
RsaNI	GTAC	4 cut(s)	47, 157, 258, 671
RseI	CAYNNNNRTG	2 cut(s)	435, 463
SalI	GTCGAC	1 cut(s)	887
SaqAI	TTAA	1 cut(s)	69
SatI	GCNGC	2 cut(s)	364, 679
Sau3AI	GATC	5 cut(s)	3, 7, 175, 307, 703
Sau96I	GGNCC	6 cut(s)	36, 216, 217, 284, 473, 836
SchI	GAGTC	3 cut(s)	31, 139, 894
ScrFI	CCNGG	5 cut(s)	114, 221, 675, 852, 871
SduI	GDGCHC	4 cut(s)	220, 460, 555, 607
SexAI	ACCWGGT	1 cut(s)	850
SfaNI	GCATC	1 cut(s)	427
SfcI	CTRYAG	1 cut(s)	179
SinI	GGWCC	3 cut(s)	284, 473, 836
SmiMI	CAYNNNNRTG	2 cut(s)	435, 463
SmlI	CTYRAG	3 cut(s)	14, 127, 207
SmoI	CTYRAG	3 cut(s)	14, 127, 207
SpeI	ACTAGT	1 cut(s)	808
Sse9I	AATT	3 cut(s)	168, 201, 417
SsiI	CCGC	1 cut(s)	839
SspI	AATATT	1 cut(s)	920
SspMI	CTAG	5 cut(s)	53, 356, 401, 800, 809
StyD4I	CCNGG	5 cut(s)	112, 219, 673, 850, 869
TaaI	ACNGT	2 cut(s)	46, 319
TaiI	ACGT	1 cut(s)	558
TaqI	TCGA	2 cut(s)	6, 888
TaqII	GACCGA	1 cut(s)	301
TasI	AATT	3 cut(s)	168, 201, 417
TatI	WGTACW	3 cut(s)	46, 156, 257
Tru1I	TTAA	1 cut(s)	69
Tru9I	TTAA	1 cut(s)	69
TscAI	CASTG	1 cut(s)	544
TseFI	GTSAC	4 cut(s)	334, 572, 595, 865
TseI	GCWGC	2 cut(s)	363, 678
Tsp45I	GTSAC	4 cut(s)	334, 572, 595, 865
TspDTI	ATGAA	1 cut(s)	744
TspGWI	ACGGA	1 cut(s)	755
TspRI	CASTG	1 cut(s)	544
VneI	GTGCAC	1 cut(s)	456
VpaK11BI	GGWCC	3 cut(s)	284, 473, 836
XagI	CCTNNNNNAGG	1 cut(s)	336
XapI	RAATTY	1 cut(s)	417
XbaI	TCTAGA	1 cut(s)	355
XmiI	GTMKAC	1 cut(s)	888
XmnI	GAANNNNTTC	1 cut(s)	521
XspI	CTAG	5 cut(s)	53, 356, 401, 800, 809

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.