Rroxscaffold_1G00064310

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003665
Physical Location & Seq
Reverse (-)
86116405 .. 86121366
4962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064310.1

Sequence Viewer

Length: 1671 bp
ATGGCAACAAGAAGCTCGAGCTCAGAAGCTTTGACGACCACTCTGTCCAACTCAATCCAAGCTCTGGGTCGAGGCTTTGATGTGACTTCGGATATTAGGCTTCTGTATTGCAAAGGAGCACCTGGGTCAAGGCTTGTGAATCTGGATGAGAGTGGGGCTAAAGATCTTGCTGTTTCTGATGGGGTTGTGGTGCCAAATGTGCCTGCAGATATTGACTGCTCACCTGGGAAGAGAACCATTGAGAGAGTCCCTGTCTGTAGCTTCCATGAGATGGCAGAATACTTTAATAAGAAATCAGGTATATCAACCCGAATTCCCCTGGGAAGCTTCAATGCCATGTTTAATTTCACCGGTTCTTGGCAAGTTGATGCAGCAGCAACCAAATCCCTTGCTATGGTTGGATACTTTATTCCCCTATACAAAGTCAACTTAGAAACAGAAAATTTGGTTTTGCATGATGAAATCAAGCGTGCTGTTCCTTACTCTTGGGATCCTGCAGCCTTGGCAAGTTTCATCGAGAACTTTGGTACACATATAGTCACATCTGCGACAATTGGTGGGAGAGATGTGGTCTATATCAGGCAGCACCAGTCATCTCCTTTATCATTATCGGACATTCAGAATTATGTGAAAGACATTGGTGATGATAGGTTTCTGGACTCAAAAGCCCAGTCAAGTGCTGGCCCCTTAAAATACAAGGACAAGGATGTTACAGTCATCTTCAGGAGAAGGGGAGGTGATGATCTTGAGCAGAGTCATTCCAAGTGGGCGGAGACAGTACAACTAGCACCAGATGTGATCAATATGATGTTTACACCTATTGTTTCTCTTCTTGAAGGAGTGCCTGGAATAAAGCACTTGAGCCGTGCAATTGAGTTATATCTAGAGTACAAGCCTCCAATTGAAGATCTGCAGTATTTCTTGGACTTTCAAATTGCTCAGGTTTGGGCCCCAGAGCAAAATAATCTGCAAAGGAAAGAACCTGTCTGTTCATCCCTTCAATTCAGCTTGATGGGTCCCAAGCTTTATATCAGTCCAGATCAGGTAACAGTAGGCCGTAAGCCTGTCACAGGGCTCAGGCTTAACCTAGAAGGCAACAAGCAGAACCGACTTGCTATACATTTGCAGCATCTAGTGTCCCTTCCCAAGATTCTTCAACCCCATTGGGATGCCCATATGGCCATAGGTGCACCTAGGTGGCAAGGTCCAGAGGAGCAAGACAGTCGTTGGTTTGAGCCGATCAAGTGGAAGAACTTCTCCCACGTAAGCACTGCACCAATAGAGCACGTAGAGACTTCTATTGGTGACCTCTCTGGTGTTAACATTGTCACAGGCGCTCAGCTTGGTGTATGGGATTTTGGTGCCAAAAATGTTTTGCACCTCAAACTCCTCTTTTCAAAAGTACCAGGCTGCACAATCCGACGGTCTGTGTGGGATCACAGTCCCGCCGCCCCTTCTTCTGCACAGAGGCCTGATGGTTCTTCTTCATCACATTCAAATAGCAGAAACTCTGATGACAAAAAGGACAAACTAGCAAAGTTAGTAGACTTGACAGAAATGTCAAAGGGGCCGCAAGATATTCCTGGACATTGGTTAGTCACAGGAGCTAAGCTTGGAGTGGACAAGGGAAAGATTGTATTGCGTGTAAAATATTCCTTGCTAAACTACTGA

Protein Analysis

556

Amino Acids

61.5

Weight (kDa)

7.7

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 108 - 294 8.6e-31 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 43, 1558
AccB1I GGYRCC 2 cut(s) 190, 1361
AccB7I CCANNNNNTGG 2 cut(s) 64, 271
AccI GTMKAC 1 cut(s) 1545
AciI CCGC 4 cut(s) 770, 1446, 1449, 1571
AclWI GGATC 3 cut(s) 485, 498, 1443
AcoI YGGCCR 1 cut(s) 1179
AcsI RAATTY 2 cut(s) 312, 442
AcuI CTGAAG 1 cut(s) 706
AfaI GTAC 4 cut(s) 529, 780, 890, 1404
AfiI CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1070
AgeI ACCGGT 1 cut(s) 350
AgsI TTSAA 8 cut(s) 331, 836, 905, 932, 1001, 1157, 1398, 1497
AjnI CCWGG 6 cut(s) 121, 223, 318, 844, 1405, 1582
AleI CACNNNNGTG 1 cut(s) 1195
Alw21I GWGCWC 4 cut(s) 23, 121, 1192, 1287
Alw26I GTCTC 2 cut(s) 767, 1286
Alw44I GTGCAC 1 cut(s) 1188
AlwI GGATC 3 cut(s) 485, 498, 1443
Ama87I CYCGRG 1 cut(s) 16
AoxI GGCC 6 cut(s) 682, 948, 1054, 1179, 1469, 1568
ApaI GGGCCC 1 cut(s) 952
ApaLI GTGCAC 1 cut(s) 1188
ApeKI GCWGC 6 cut(s) 371, 374, 497, 583, 1126, 1410
ApoI RAATTY 2 cut(s) 312, 442
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AsiGI ACCGGT 1 cut(s) 350
Asp700I GAANNNNTTC 1 cut(s) 1253
AspA2I CCTAGG 1 cut(s) 1193
AspLEI GCGC 1 cut(s) 1337
AspS9I GGNCC 6 cut(s) 683, 948, 949, 1016, 1205, 1568
AsuHPI GGTGA 5 cut(s) 213, 340, 653, 749, 1316
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 2 cut(s) 1016, 1205
AvrII CCTAGG 1 cut(s) 1193
BaeGI GKGCMC 2 cut(s) 952, 1192
BalI TGGCCA 1 cut(s) 1181
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 2 cut(s) 190, 1361
BanII GRGCYC 3 cut(s) 23, 952, 1077
Bbv12I GWGCWC 4 cut(s) 23, 121, 1192, 1287
BbvI GCAGC 6 cut(s) 383, 386, 509, 595, 1138, 1397
BccI CCATC 4 cut(s) 173, 265, 1006, 1469
BceAI ACGGC 2 cut(s) 849, 1041
BcgI CGANNNNNNTGC 2 cut(s) 1205, 1239
BciT130I CCWGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
BciVI GTATCC 1 cut(s) 395
BclI TGATCA 1 cut(s) 798
BcoDI GTCTC 2 cut(s) 767, 1286
BfaI CTAG 6 cut(s) 785, 884, 1088, 1133, 1194, 1532
BfmI CTRYAG 4 cut(s) 204, 256, 495, 911
BfoI RGCGCY 1 cut(s) 1338
BfuI GTATCC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 1178
BglII AGATCT 2 cut(s) 163, 907
BisI GCNGC 8 cut(s) 372, 375, 498, 584, 1127, 1411, 1449, 1571
BlnI CCTAGG 1 cut(s) 1193
BlpI GCTNAGC 2 cut(s) 1338, 1608
BlsI GCNGC 8 cut(s) 373, 376, 499, 585, 1128, 1412, 1450, 1572
Bme1390I CCNGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
Bme18I GGWCC 2 cut(s) 1016, 1205
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 6 cut(s) 683, 948, 949, 1016, 1205, 1568
BmiI GGNNCC 9 cut(s) 192, 492, 685, 950, 951, 1017, 1018, 1363, 1569
BmrFI CCNGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 3 cut(s) 358, 1138, 1159
BmuI ACTGGG 1 cut(s) 664
Bpu10I CCTNAGC 2 cut(s) 939, 1076
Bpu1102I GCTNAGC 2 cut(s) 1338, 1608
BpuEI CTTGAG 2 cut(s) 767, 880
BsaAI YACGTR 2 cut(s) 1264, 1288
BsaJI CCNNGG 6 cut(s) 122, 224, 318, 319, 501, 1193
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1070
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 2 cut(s) 589, 670
BseBI CCWGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
BseDI CCNNGG 6 cut(s) 122, 224, 318, 319, 501, 1193
BseGI GGATG 4 cut(s) 151, 712, 992, 1174
BseLI CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1070
BseMII CTCAG 4 cut(s) 36, 953, 1090, 1352
BseNI ACTGG 2 cut(s) 589, 670
BseRI GAGGAG 2 cut(s) 1226, 1379
BseSI GKGCMC 2 cut(s) 952, 1192
BseXI GCAGC 6 cut(s) 383, 386, 509, 595, 1138, 1397
BsgI GTGCAG 3 cut(s) 1257, 1396, 1446
BshFI GGCC 6 cut(s) 684, 950, 1056, 1181, 1471, 1570
BshNI GGYRCC 2 cut(s) 190, 1361
BshTI ACCGGT 1 cut(s) 350
BsiHKAI GWGCWC 4 cut(s) 23, 121, 1192, 1287
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 4 cut(s) 233, 1002, 1123, 1428
BslI CCNNNNNNNGG 5 cut(s) 64, 271, 357, 394, 1070
BsmAI GTCTC 2 cut(s) 767, 1286
BsmFI GGGAC 4 cut(s) 233, 1002, 1123, 1428
BsnI GGCC 6 cut(s) 684, 950, 1056, 1181, 1471, 1570
BsoBI CYCGRG 1 cut(s) 16
Bsp120I GGGCCC 1 cut(s) 948
Bsp1286I GDGCHC 6 cut(s) 23, 121, 952, 1077, 1192, 1287
Bsp143I GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
Bsp1720I GCTNAGC 2 cut(s) 1338, 1608
BspACI CCGC 4 cut(s) 770, 1446, 1449, 1571
BspANI GGCC 6 cut(s) 684, 950, 1056, 1181, 1471, 1570
BspCNI CTCAG 4 cut(s) 35, 952, 1089, 1351
BspLI GGNNCC 9 cut(s) 192, 492, 685, 950, 951, 1017, 1018, 1363, 1569
BspMAI CTGCAG 3 cut(s) 208, 499, 915
BspPI GGATC 3 cut(s) 485, 498, 1443
BspT107I GGYRCC 2 cut(s) 190, 1361
BsrFI RCCGGY 1 cut(s) 350
BsrI ACTGG 2 cut(s) 589, 670
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 6 cut(s) 122, 224, 318, 319, 501, 1193
BssMI GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
BssT1I CCWWGG 2 cut(s) 501, 1193
Bst2UI CCWGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
Bst4CI ACNGT 6 cut(s) 715, 778, 1051, 1223, 1425, 1442
Bst6I CTCTTC 2 cut(s) 224, 834
BstBAI YACGTR 2 cut(s) 1264, 1288
BstC8I GCNNGC 3 cut(s) 204, 471, 682
BstDEI CTNAG 6 cut(s) 22, 430, 939, 1076, 1338, 1608
BstEII GGTNACC 1 cut(s) 1304
BstENI CCTNNNNNAGG 1 cut(s) 1068
BstF5I GGATG 4 cut(s) 151, 712, 992, 1174
BstH2I RGCGCY 1 cut(s) 1338
BstHHI GCGC 1 cut(s) 1337
BstKTI GATC 8 cut(s) 166, 493, 745, 801, 910, 1042, 1242, 1438
BstMAI GTCTC 2 cut(s) 767, 1286
BstMBI GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
BstMWI GCNNNNNNNGC 4 cut(s) 199, 503, 1178, 1187
BstNI CCWGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
BstPI GGTNACC 1 cut(s) 1304
BstSCI CCNGG 6 cut(s) 121, 223, 318, 844, 1405, 1582
BstSFI CTRYAG 4 cut(s) 204, 256, 495, 911
BstSLI GKGCMC 2 cut(s) 952, 1192
BstV1I GCAGC 6 cut(s) 383, 386, 509, 595, 1138, 1397
BstX2I RGATCY 3 cut(s) 163, 490, 907
BstYI RGATCY 3 cut(s) 163, 490, 907
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 6 cut(s) 684, 950, 1056, 1181, 1471, 1570
BtsCI GGATG 4 cut(s) 151, 712, 992, 1174
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 1 cut(s) 1269
Cac8I GCNNGC 3 cut(s) 204, 471, 682
CfoI GCGC 1 cut(s) 1337
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 6 cut(s) 683, 948, 949, 1016, 1205, 1568
Csp6I GTAC 4 cut(s) 528, 779, 889, 1403
CspAI ACCGGT 1 cut(s) 350
CviAII CATG 3 cut(s) 266, 337, 455
CviQI GTAC 4 cut(s) 528, 779, 889, 1403
DdeI CTNAG 6 cut(s) 22, 430, 939, 1076, 1338, 1608
DpnI GATC 8 cut(s) 165, 492, 744, 800, 909, 1041, 1241, 1437
DpnII GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
DrdI GACNNNNNNGTC 2 cut(s) 43, 1558
DseDI GACNNNNNNGTC 2 cut(s) 43, 1558
EaeI YGGCCR 1 cut(s) 1179
Eam1104I CTCTTC 2 cut(s) 224, 834
EarI CTCTTC 2 cut(s) 224, 834
EciI GGCGGA 1 cut(s) 785
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 501, 1193
Eco147I AGGCCT 1 cut(s) 1471
Eco24I GRGCYC 3 cut(s) 23, 952, 1077
Eco47I GGWCC 2 cut(s) 1016, 1205
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 706
Eco88I CYCGRG 1 cut(s) 16
Eco91I GGTNACC 1 cut(s) 1304
EcoICRI GAGCTC 1 cut(s) 21
EcoNI CCTNNNNNAGG 1 cut(s) 1068
EcoO109I RGGNCCY 2 cut(s) 949, 1016
EcoO65I GGTNACC 1 cut(s) 1304
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 6 cut(s) 121, 223, 318, 844, 1405, 1582
EcoT14I CCWWGG 2 cut(s) 501, 1193
EcoT38I GRGCYC 3 cut(s) 23, 952, 1077
ErhI CCWWGG 2 cut(s) 501, 1193
FaeI CATG 3 cut(s) 269, 340, 458
FaqI GGGAC 4 cut(s) 233, 1002, 1123, 1428
FatI CATG 3 cut(s) 265, 336, 454
FauI CCCGC 1 cut(s) 1453
FauNDI CATATG 1 cut(s) 1176
FbaI TGATCA 1 cut(s) 798
FblI GTMKAC 1 cut(s) 1545
Fnu4HI GCNGC 8 cut(s) 372, 375, 498, 584, 1127, 1411, 1449, 1571
FokI GGATG 4 cut(s) 158, 719, 979, 1181
FriOI GRGCYC 3 cut(s) 23, 952, 1077
Fsp4HI GCNGC 8 cut(s) 372, 375, 498, 584, 1127, 1411, 1449, 1571
FspBI CTAG 6 cut(s) 785, 884, 1088, 1133, 1194, 1532
GlaI GCGC 1 cut(s) 1336
GluI GCNGC 8 cut(s) 372, 375, 498, 584, 1127, 1411, 1449, 1571
HaeII RGCGCY 1 cut(s) 1338
HaeIII GGCC 6 cut(s) 684, 950, 1056, 1181, 1471, 1570
HapII CCGG 1 cut(s) 351
HhaI GCGC 1 cut(s) 1337
Hin1II CATG 3 cut(s) 269, 340, 458
Hin6I GCGC 1 cut(s) 1335
HinP1I GCGC 1 cut(s) 1335
HincII GTYRAC 2 cut(s) 427, 1321
HindII GTYRAC 2 cut(s) 427, 1321
HindIII AAGCTT 4 cut(s) 27, 325, 1022, 1610
HinfI GANTC 5 cut(s) 139, 246, 659, 754, 1150
HpaI GTTAAC 1 cut(s) 1321
HpaII CCGG 1 cut(s) 351
HphI GGTGA 5 cut(s) 213, 340, 653, 749, 1316
Hpy166II GTNNAC 7 cut(s) 427, 530, 813, 1190, 1321, 1546, 1621
Hpy188I TCNGA 7 cut(s) 25, 91, 178, 613, 621, 1421, 1513
Hpy188III TCNNGA 9 cut(s) 143, 517, 656, 724, 746, 833, 884, 1037, 1208
Hpy8I GTNNAC 7 cut(s) 427, 530, 813, 1190, 1321, 1546, 1621
Hpy99I CGWCG 1 cut(s) 1425
HpyAV CCTTC 6 cut(s) 723, 830, 1007, 1085, 1151, 1464
HpyCH4III ACNGT 6 cut(s) 715, 778, 1051, 1223, 1425, 1442
HpyCH4IV ACGT 2 cut(s) 1263, 1287
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 503, 1178, 1187
HpyF3I CTNAG 6 cut(s) 22, 430, 939, 1076, 1338, 1608
HpySE526I ACGT 2 cut(s) 1263, 1287
Hsp92II CATG 3 cut(s) 269, 340, 458
HspAI GCGC 1 cut(s) 1335
KflI GGGWCCC 1 cut(s) 1016
Ksp22I TGATCA 1 cut(s) 798
KspAI GTTAAC 1 cut(s) 1321
Kzo9I GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
LmnI GCTCC 3 cut(s) 116, 1213, 1604
Lsp1109I GCAGC 6 cut(s) 383, 386, 509, 595, 1138, 1397
LweI GCATC 3 cut(s) 358, 1138, 1159
MaeI CTAG 6 cut(s) 785, 884, 1088, 1133, 1194, 1532
MaeII ACGT 2 cut(s) 1263, 1287
MaeIII GTNAC 8 cut(s) 82, 538, 709, 1045, 1066, 1304, 1327, 1597
MalI GATC 8 cut(s) 165, 492, 744, 800, 909, 1041, 1241, 1437
MboI GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
MboII GAAGA 9 cut(s) 241, 712, 821, 917, 1145, 1261, 1449, 1473, 1476
MfeI CAATTG 3 cut(s) 552, 870, 900
MflI RGATCY 3 cut(s) 163, 490, 907
MhlI GDGCHC 6 cut(s) 23, 121, 952, 1077, 1192, 1287
MlsI TGGCCA 1 cut(s) 1181
MluCI AATT 9 cut(s) 312, 343, 442, 552, 622, 870, 900, 933, 1001
MluNI TGGCCA 1 cut(s) 1181
MlyI GAGTC 3 cut(s) 255, 653, 763
MmeI TCCRAC 3 cut(s) 72, 379, 1444
MnlI CCTC 8 cut(s) 65, 728, 906, 1204, 1319, 1391, 1400, 1461
Mox20I TGGCCA 1 cut(s) 1181
MroXI GAANNNNTTC 1 cut(s) 1253
MscI TGGCCA 1 cut(s) 1181
MseI TTAA 5 cut(s) 285, 342, 689, 1083, 1320
MslI CAYNNNNRTG 2 cut(s) 1167, 1195
Msp20I TGGCCA 1 cut(s) 1181
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
MunI CAATTG 3 cut(s) 552, 870, 900
MvaI CCWGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
MwoI GCNNNNNNNGC 4 cut(s) 199, 503, 1178, 1187
NdeI CATATG 1 cut(s) 1176
NdeII GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
NlaIII CATG 3 cut(s) 269, 340, 458
NlaIV GGNNCC 9 cut(s) 192, 492, 685, 950, 951, 1017, 1018, 1363, 1569
NmuCI GTSAC 6 cut(s) 82, 538, 1066, 1304, 1327, 1597
OliI CACNNNNGTG 1 cut(s) 1195
PaeR7I CTCGAG 1 cut(s) 16
PasI CCCWGGG 1 cut(s) 319
PceI AGGCCT 1 cut(s) 1471
PdmI GAANNNNTTC 1 cut(s) 1253
PfeI GAWTC 2 cut(s) 139, 1150
PflMI CCANNNNNTGG 2 cut(s) 64, 271
PfoI TCCNGGA 1 cut(s) 1582
PinAI ACCGGT 1 cut(s) 350
PkrI GCNGC 8 cut(s) 373, 376, 499, 585, 1128, 1412, 1450, 1572
PleI GAGTC 3 cut(s) 254, 653, 762
PpsI GAGTC 3 cut(s) 254, 653, 762
Ppu21I YACGTR 2 cut(s) 1264, 1288
PpuMI RGGWCCY 1 cut(s) 1016
Psp124BI GAGCTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 1016
Psp6I CCWGG 6 cut(s) 121, 223, 318, 844, 1405, 1582
PspEI GGTNACC 1 cut(s) 1304
PspGI CCWGG 6 cut(s) 121, 223, 318, 844, 1405, 1582
PspN4I GGNNCC 9 cut(s) 192, 492, 685, 950, 951, 1017, 1018, 1363, 1569
PspOMI GGGCCC 1 cut(s) 948
PspPI GGNCC 6 cut(s) 683, 948, 949, 1016, 1205, 1568
PspPPI RGGWCCY 1 cut(s) 1016
PspXI VCTCGAGB 1 cut(s) 16
PstI CTGCAG 3 cut(s) 208, 499, 915
PsuI RGATCY 3 cut(s) 163, 490, 907
RsaI GTAC 4 cut(s) 529, 780, 890, 1404
RsaNI GTAC 4 cut(s) 528, 779, 889, 1403
RseI CAYNNNNRTG 2 cut(s) 1167, 1195
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 5 cut(s) 285, 342, 689, 1083, 1320
SatI GCNGC 8 cut(s) 372, 375, 498, 584, 1127, 1411, 1449, 1571
Sau3AI GATC 8 cut(s) 163, 490, 742, 798, 907, 1039, 1239, 1435
Sau96I GGNCC 6 cut(s) 683, 948, 949, 1016, 1205, 1568
SchI GAGTC 3 cut(s) 255, 653, 763
ScrFI CCNGG 6 cut(s) 123, 225, 320, 846, 1407, 1584
SduI GDGCHC 6 cut(s) 23, 121, 952, 1077, 1192, 1287
SfaNI GCATC 3 cut(s) 358, 1138, 1159
SfcI CTRYAG 4 cut(s) 204, 256, 495, 911
Sfr274I CTCGAG 1 cut(s) 16
SinI GGWCC 2 cut(s) 1016, 1205
SlaI CTCGAG 1 cut(s) 16
SmiMI CAYNNNNRTG 2 cut(s) 1167, 1195
SmlI CTYRAG 3 cut(s) 16, 746, 859
SmoI CTYRAG 3 cut(s) 16, 746, 859
Sse9I AATT 9 cut(s) 312, 343, 442, 552, 622, 870, 900, 933, 1001
SseBI AGGCCT 1 cut(s) 1471
SsiI CCGC 4 cut(s) 770, 1446, 1449, 1571
SspI AATATT 1 cut(s) 1652
SspMI CTAG 6 cut(s) 785, 884, 1088, 1133, 1194, 1532
SstI GAGCTC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 1471
StyD4I CCNGG 6 cut(s) 121, 223, 318, 844, 1405, 1582
StyI CCWWGG 2 cut(s) 501, 1193
TaaI ACNGT 6 cut(s) 715, 778, 1051, 1223, 1425, 1442
TaiI ACGT 2 cut(s) 1266, 1290
TaqI TCGA 3 cut(s) 17, 70, 516
TasI AATT 9 cut(s) 312, 343, 442, 552, 622, 870, 900, 933, 1001
TatI WGTACW 2 cut(s) 778, 888
TauI GCSGC 2 cut(s) 1451, 1573
TfiI GAWTC 2 cut(s) 139, 1150
Tru1I TTAA 5 cut(s) 285, 342, 689, 1083, 1320
Tru9I TTAA 5 cut(s) 285, 342, 689, 1083, 1320
TscAI CASTG 1 cut(s) 1276
TseFI GTSAC 6 cut(s) 82, 538, 1066, 1304, 1327, 1597
TseI GCWGC 6 cut(s) 371, 374, 497, 583, 1126, 1410
Tsp45I GTSAC 6 cut(s) 82, 538, 1066, 1304, 1327, 1597
TspDTI ATGAA 4 cut(s) 474, 502, 981, 1476
TspRI CASTG 1 cut(s) 1276
Van91I CCANNNNNTGG 2 cut(s) 64, 271
VneI GTGCAC 1 cut(s) 1188
VpaK11BI GGWCC 2 cut(s) 1016, 1205
XagI CCTNNNNNAGG 1 cut(s) 1068
XapI RAATTY 2 cut(s) 312, 442
XbaI TCTAGA 1 cut(s) 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XhoI CTCGAG 1 cut(s) 16
XmaJI CCTAGG 1 cut(s) 1193
XmiI GTMKAC 1 cut(s) 1545
XmnI GAANNNNTTC 1 cut(s) 1253
XspI CTAG 6 cut(s) 785, 884, 1088, 1133, 1194, 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.