Rroxscaffold_6G00419290

MACPF domain-containing protein CAD1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003665
Physical Location & Seq
Forward (+)
40775096 .. 40780320
5225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00419290.1

Sequence Viewer

Length: 1755 bp
ATGGGGGAAAATGTAGCAGCTTTGCACACTGCTGTTAACTCTGTACAGGCACTGGGAAGAGGGTTTGACGTGAACTTTGATACGAGGCTGCTTTATTGTAAGGGTGTGGCAGGGTCTAGGGTACTAGAGGTTGATGAGGAGCACACCAGGGACCTCTGCCTTTATGATGATGTAGTTGTGCCCAATGTTTCCAAGGATATTAAGCGCTCTCAGGAGTCCAGTGAGCGGCGGAGTTCTGGAGTCTGCAGCTTCAATGAGATGGTTGAGTATTTTAATCAAAAGTCTAATATATCTGGAGGCTATCCTGTGGGGAGTTTCAATTTTGCATTTAGCTTCAGCGGTTCAAAGCATGTTGATATGGCAGGAACAAAGACACTTTCTATGGATGGCCTCTATGTTCCACTTGCTAATATCCATCTTTTAAAATCCCCACTAATGTTACAAGAAAATGTGAAACAGGCTATCCCAACTTGTTGGGATCCCAAATCCTTGGCAAGCTTTATTGAAAATTTTGGTACTCATGTCATAACATCTGTAACTATTGGTGGCAAAGATGTAATTTACGTTAAACAACACCAGTCTTCTCCTTTGTCAACCATGGAGATCAAGAACTACATCCAAGACATTGGAAATCAGAGGTTCTCAGACACAGAAAGCAATACAAGTTCAGTTAAAATGAATTTCAAGGATAAGGGGTCTGATTCCGGTTTATTCAACAGTCAGGGGATATACCCTCAACCCACCACTGCACCACATCTTGCTGGGAAAGAAGATGTGACTGTCATTTTCCGAAGAAGGGGAGGAGATGATCTGGAACAAAACCACACCCGCTGGGCAAGAACTGTCCGGTCCTCTCCAGATGTTATTGAGATGACGTTTTTTCCAATAACAGCTCTCCTTGAAGGGGTAATTGGAAGGGAGCACCTTACTCGAGCTATTGGCCTTTACCTTGAATATAAACCTCCAATAGAAGAACTGAGATACTTCCTAGAGTTTCAAATTTCTCGAATATGGGCTCCTGTATATGACAGAAATCTTACCCACCAAAGAAAGGAACCTGTTTGCCCATCTTTGCAATTCAGCATAATGGGGCAAAAACTCTATGTCAGCCAAGAGCAGATAACAGTTGGACGGAAGCCAGTGACAGGCTTGCGGTTATGTCTCGAAGGAAGCAAGCAGAATCATCTTTGTATCCATCTTCAACACTTGGCATCTCTTCCAAAGATCCTTCTACCATATTGGGATTCCCATGTAGCAATTGGTGCTCCAAAGTGGCAAGGACCTGAGGAGCAGGATAGCCGATGGTTCGAGCCAGTGAAGTGGAAAAACTTCTCTCATGTGAGCACAACACCTATTGAGAACCCTGAAACCTTCATAGGCGATCTCTCTGGTGTCTACATTGTCACAGGAGCTCAGCTTGGAGTGTGGGATTTCGGATCAAAAAATGTTTTGTACATGAAGCTATTATATTCCAGGCTGCCTGGCTGCACTATACGAAGATCCTTGTGGGACCATTCCCCAAATGAAAAGTCAAAGAAAGGGACGTCTACTAGTTTAAATGTCAATGCCAGTGACTCAAGTTCCGGTTCAAAAGAAACTATTGCGGGTAACAAGTTGATGAAATTTTTGGATATGTCCGAGATGAGCAAGGGGCCACAAGATCATCCAGGTCATTGGCTGGTTACTGGTGGAAAGCTTGGTGTGGAAAAAGGAAAAATTGTCATAAGAGTGAAATATTCGCTGCTAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

584

Amino Acids

65.4

Weight (kDa)

8.42

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 104 - 316 2.1e-29 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1547
AccBSI CCGCTC 1 cut(s) 226
AccI GTMKAC 2 cut(s) 1395, 1547
AciI CCGC 6 cut(s) 226, 229, 339, 829, 1153, 1604
AclWI GGATC 5 cut(s) 473, 486, 1219, 1444, 1494
AcsI RAATTY 4 cut(s) 508, 679, 999, 1622
AcuI CTGAAG 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 1544
AfaI GTAC 4 cut(s) 45, 123, 517, 1454
AfeI AGCGCT 1 cut(s) 206
AfiI CCNNNNNNNGG 5 cut(s) 225, 796, 904, 1051, 1145
AhlI ACTAGT 1 cut(s) 1550
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 4 cut(s) 146, 1472, 1480, 1666
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
Alw21I GWGCWC 5 cut(s) 144, 924, 1267, 1346, 1414
Alw26I GTCTC 1 cut(s) 1166
AlwI GGATC 5 cut(s) 473, 486, 1219, 1444, 1494
AlwNI CAGNNNCTG 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 930
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 3 cut(s) 388, 940, 1652
ApeKI GCWGC 6 cut(s) 17, 88, 246, 1477, 1485, 1741
ApoI RAATTY 4 cut(s) 508, 679, 999, 1622
ArsI GACNNNNNNTTYG 4 cut(s) 59, 91, 1514, 1546
Asp700I GAANNNNTTC 1 cut(s) 1328
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 5 cut(s) 151, 849, 1280, 1510, 1652
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 4 cut(s) 151, 849, 1280, 1510
AxyI CCTNAGG 1 cut(s) 1284
BaeGI GKGCMC 1 cut(s) 183
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BamHI GGATCC 1 cut(s) 478
BanII GRGCYC 2 cut(s) 1018, 1414
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 5 cut(s) 144, 924, 1267, 1346, 1414
BbvI GCAGC 6 cut(s) 29, 75, 258, 1464, 1472, 1728
BccI CCATC 6 cut(s) 253, 380, 423, 1075, 1203, 1296
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 4 cut(s) 148, 1474, 1482, 1668
BciVI GTATCC 1 cut(s) 1202
BcoDI GTCTC 1 cut(s) 1166
BcuI ACTAGT 1 cut(s) 1550
BfaI CTAG 4 cut(s) 117, 125, 989, 1551
BfmI CTRYAG 1 cut(s) 244
BfoI RGCGCY 1 cut(s) 208
BfuI GTATCC 1 cut(s) 1202
BisI GCNGC 7 cut(s) 18, 89, 227, 247, 1478, 1486, 1742
BlpI GCTNAGC 1 cut(s) 1413
BlsI GCNGC 7 cut(s) 19, 90, 228, 248, 1479, 1487, 1743
Bme1390I CCNGG 4 cut(s) 148, 1474, 1482, 1668
Bme18I GGWCC 4 cut(s) 151, 849, 1280, 1510
BmeT110I CYCGRG 1 cut(s) 930
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 5 cut(s) 151, 849, 1280, 1510, 1652
BmiI GGNNCC 6 cut(s) 152, 480, 1017, 1056, 1511, 1653
BmrFI CCNGG 4 cut(s) 148, 1474, 1482, 1668
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 1220
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 573
BpmI CTGGAG 3 cut(s) 258, 315, 840
Bpu1102I GCTNAGC 1 cut(s) 1413
BpuEI CTTGAG 1 cut(s) 1561
BsaHI GRCGYC 1 cut(s) 1544
BsaJI CCNNGG 4 cut(s) 147, 192, 489, 597
BsaWI WCCGGW 3 cut(s) 704, 846, 1583
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 796, 904, 1051, 1145
Bse1I ACTGG 7 cut(s) 57, 219, 577, 1139, 1313, 1569, 1690
Bse21I CCTNAGG 1 cut(s) 1284
BseBI CCWGG 4 cut(s) 148, 1474, 1482, 1668
BseDI CCNNGG 4 cut(s) 147, 192, 489, 597
BseGI GGATG 3 cut(s) 391, 615, 1663
BseLI CCNNNNNNNGG 5 cut(s) 225, 796, 904, 1051, 1145
BseMII CTCAG 5 cut(s) 224, 657, 968, 1275, 1427
BseNI ACTGG 7 cut(s) 57, 219, 577, 1139, 1313, 1569, 1690
BseRI GAGGAG 3 cut(s) 152, 816, 1301
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 6 cut(s) 29, 75, 258, 1464, 1472, 1728
BseYI CCCAGC 2 cut(s) 761, 831
BsgI GTGCAG 2 cut(s) 732, 1471
BshFI GGCC 3 cut(s) 390, 942, 1654
BsiHKAI GWGCWC 5 cut(s) 144, 924, 1267, 1346, 1414
BsiHKCI CYCGRG 1 cut(s) 930
BsiSI CCGG 3 cut(s) 705, 847, 1584
BslFI GGGAC 3 cut(s) 164, 1523, 1555
BslI CCNNNNNNNGG 5 cut(s) 225, 796, 904, 1051, 1145
BsmAI GTCTC 1 cut(s) 1166
BsmFI GGGAC 3 cut(s) 164, 1523, 1555
BsnI GGCC 3 cut(s) 390, 942, 1654
BsoBI CYCGRG 1 cut(s) 930
Bsp1286I GDGCHC 7 cut(s) 144, 183, 924, 1018, 1267, 1346, 1414
Bsp1407I TGTACA 2 cut(s) 43, 1452
Bsp143I GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
Bsp1720I GCTNAGC 1 cut(s) 1413
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 6 cut(s) 226, 229, 339, 829, 1153, 1604
BspANI GGCC 3 cut(s) 390, 942, 1654
BspCNI CTCAG 5 cut(s) 223, 656, 969, 1276, 1426
BspLI GGNNCC 6 cut(s) 152, 480, 1017, 1056, 1511, 1653
BspMAI CTGCAG 1 cut(s) 248
BspPI GGATC 5 cut(s) 473, 486, 1219, 1444, 1494
BsrBI CCGCTC 1 cut(s) 226
BsrGI TGTACA 2 cut(s) 43, 1452
BsrI ACTGG 7 cut(s) 57, 219, 577, 1139, 1313, 1569, 1690
BssECI CCNNGG 4 cut(s) 147, 192, 489, 597
BssMI GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
BssNI GRCGYC 1 cut(s) 1544
BssT1I CCWWGG 3 cut(s) 192, 489, 597
Bst2UI CCWGG 4 cut(s) 148, 1474, 1482, 1668
Bst4CI ACNGT 4 cut(s) 719, 781, 844, 1126
Bst6I CTCTTC 2 cut(s) 52, 1221
BstACI GRCGYC 1 cut(s) 1544
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstAUI TGTACA 2 cut(s) 43, 1452
BstC8I GCNNGC 3 cut(s) 496, 1151, 1175
BstDEI CTNAG 5 cut(s) 210, 643, 977, 1284, 1413
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 3 cut(s) 391, 615, 1663
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 8 cut(s) 481, 606, 811, 1227, 1384, 1439, 1502, 1663
BstMAI GTCTC 1 cut(s) 1166
BstMBI GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
BstMWI GCNNNNNNNGC 1 cut(s) 1262
BstNI CCWGG 4 cut(s) 148, 1474, 1482, 1668
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 4 cut(s) 146, 1472, 1480, 1666
BstSFI CTRYAG 1 cut(s) 244
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 6 cut(s) 29, 75, 258, 1464, 1472, 1728
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 3 cut(s) 478, 1224, 1499
BstXI CCANNNNNNTGG 5 cut(s) 474, 490, 626, 1320, 1674
BstYI RGATCY 3 cut(s) 478, 1224, 1499
Bsu36I CCTNAGG 1 cut(s) 1284
BsuI GTATCC 1 cut(s) 1202
BsuRI GGCC 3 cut(s) 390, 942, 1654
BtgI CCRYGG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 3 cut(s) 391, 615, 1663
BtsI GCAGTG 2 cut(s) 27, 744
BtsIMutI CAGTG 7 cut(s) 27, 50, 226, 744, 1146, 1320, 1576
Cac8I GCNNGC 3 cut(s) 496, 1151, 1175
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 5 cut(s) 151, 849, 1280, 1510, 1652
Csp6I GTAC 4 cut(s) 44, 122, 516, 1453
CviAII CATG 6 cut(s) 350, 521, 598, 1250, 1337, 1456
CviQI GTAC 4 cut(s) 44, 122, 516, 1453
DdeI CTNAG 5 cut(s) 210, 643, 977, 1284, 1413
DpnI GATC 8 cut(s) 480, 605, 810, 1226, 1383, 1438, 1501, 1662
DpnII GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
DraI TTTAAA 2 cut(s) 423, 1557
Eam1104I CTCTTC 2 cut(s) 52, 1221
EarI CTCTTC 2 cut(s) 52, 1221
EciI GGCGGA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 1412
Eco130I CCWWGG 3 cut(s) 192, 489, 597
Eco24I GRGCYC 2 cut(s) 1018, 1414
Eco47I GGWCC 4 cut(s) 151, 849, 1280, 1510
Eco47III AGCGCT 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 1412
Eco57I CTGAAG 1 cut(s) 319
Eco81I CCTNAGG 1 cut(s) 1284
Eco88I CYCGRG 1 cut(s) 930
EcoICRI GAGCTC 1 cut(s) 1412
EcoO109I RGGNCCY 2 cut(s) 151, 1280
EcoRII CCWGG 4 cut(s) 146, 1472, 1480, 1666
EcoT14I CCWWGG 3 cut(s) 192, 489, 597
EcoT38I GRGCYC 2 cut(s) 1018, 1414
ErhI CCWWGG 3 cut(s) 192, 489, 597
FaeI CATG 6 cut(s) 353, 524, 601, 1253, 1340, 1459
FaqI GGGAC 3 cut(s) 164, 1523, 1555
FatI CATG 6 cut(s) 349, 520, 597, 1249, 1336, 1455
FauI CCCGC 2 cut(s) 836, 1597
FblI GTMKAC 2 cut(s) 1395, 1547
Fnu4HI GCNGC 7 cut(s) 18, 89, 227, 247, 1478, 1486, 1742
FokI GGATG 3 cut(s) 398, 602, 1650
FriOI GRGCYC 2 cut(s) 1018, 1414
Fsp4HI GCNGC 7 cut(s) 18, 89, 227, 247, 1478, 1486, 1742
FspBI CTAG 4 cut(s) 117, 125, 989, 1551
GlaI GCGC 1 cut(s) 206
GluI GCNGC 7 cut(s) 18, 89, 227, 247, 1478, 1486, 1742
GsaI CCCAGC 2 cut(s) 765, 835
GsuI CTGGAG 3 cut(s) 258, 315, 840
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 3 cut(s) 390, 942, 1654
HapII CCGG 3 cut(s) 705, 847, 1584
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 1544
Hin1II CATG 6 cut(s) 353, 524, 601, 1253, 1340, 1459
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 2 cut(s) 37, 594
HindII GTYRAC 2 cut(s) 37, 594
HindIII AAGCTT 2 cut(s) 496, 1694
HinfI GANTC 6 cut(s) 215, 240, 701, 1180, 1244, 1574
HpaI GTTAAC 1 cut(s) 37
HpaII CCGG 3 cut(s) 705, 847, 1584
Hpy166II GTNNAC 5 cut(s) 37, 73, 594, 1396, 1548
Hpy188I TCNGA 6 cut(s) 636, 646, 700, 791, 1436, 1639
Hpy188III TCNNGA 8 cut(s) 212, 237, 294, 607, 812, 857, 1005, 1163
Hpy8I GTNNAC 5 cut(s) 37, 73, 594, 1396, 1548
HpyAV CCTTC 6 cut(s) 789, 896, 909, 1160, 1238, 1381
HpyCH4III ACNGT 4 cut(s) 719, 781, 844, 1126
HpyCH4IV ACGT 4 cut(s) 69, 564, 875, 1544
HpyCH4V TGCA 6 cut(s) 25, 246, 326, 749, 1075, 1488
HpyF10VI GCNNNNNNNGC 1 cut(s) 1262
HpyF3I CTNAG 5 cut(s) 210, 643, 977, 1284, 1413
HpySE526I ACGT 4 cut(s) 69, 564, 875, 1544
Hsp92I GRCGYC 1 cut(s) 1544
Hsp92II CATG 6 cut(s) 353, 524, 601, 1253, 1340, 1459
HspAI GCGC 1 cut(s) 205
KspAI GTTAAC 1 cut(s) 37
Kzo9I GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
LmnI GCTCC 6 cut(s) 139, 919, 1021, 1270, 1288, 1409
Lsp1109I GCAGC 6 cut(s) 29, 75, 258, 1464, 1472, 1728
LweI GCATC 1 cut(s) 1220
MaeI CTAG 4 cut(s) 117, 125, 989, 1551
MaeII ACGT 4 cut(s) 69, 564, 875, 1544
MaeIII GTNAC 8 cut(s) 438, 535, 775, 1141, 1402, 1571, 1607, 1681
MalI GATC 8 cut(s) 480, 605, 810, 1226, 1383, 1438, 1501, 1662
MbiI CCGCTC 1 cut(s) 226
MboI GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
MboII GAAGA 8 cut(s) 69, 573, 782, 804, 983, 1190, 1208, 1509
MfeI CAATTG 1 cut(s) 1257
MflI RGATCY 3 cut(s) 478, 1224, 1499
MhlI GDGCHC 7 cut(s) 144, 183, 924, 1018, 1267, 1346, 1414
MlyI GAGTC 3 cut(s) 224, 249, 1568
MmeI TCCRAC 1 cut(s) 1108
MroXI GAANNNNTTC 1 cut(s) 1328
MseI TTAA 7 cut(s) 36, 201, 273, 422, 567, 672, 1556
MslI CAYNNNNRTG 1 cut(s) 1727
MspA1I CMGCKG 2 cut(s) 339, 831
MspI CCGG 3 cut(s) 705, 847, 1584
MspR9I CCNGG 4 cut(s) 148, 1474, 1482, 1668
MunI CAATTG 1 cut(s) 1257
MvaI CCWGG 4 cut(s) 148, 1474, 1482, 1668
MwoI GCNNNNNNNGC 1 cut(s) 1262
NcoI CCATGG 1 cut(s) 597
NdeII GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
NlaIII CATG 6 cut(s) 353, 524, 601, 1253, 1340, 1459
NlaIV GGNNCC 6 cut(s) 152, 480, 1017, 1056, 1511, 1653
NmuCI GTSAC 4 cut(s) 775, 1141, 1402, 1571
NspI RCATGY 1 cut(s) 353
PaeR7I CTCGAG 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 1328
PfeI GAWTC 3 cut(s) 701, 1180, 1244
PkrI GCNGC 7 cut(s) 19, 90, 228, 248, 1479, 1487, 1743
PleI GAGTC 3 cut(s) 223, 248, 1568
PpsI GAGTC 3 cut(s) 223, 248, 1568
PpuMI RGGWCCY 2 cut(s) 151, 1280
Psp124BI GAGCTC 1 cut(s) 1414
Psp5II RGGWCCY 2 cut(s) 151, 1280
Psp6I CCWGG 4 cut(s) 146, 1472, 1480, 1666
PspFI CCCAGC 2 cut(s) 761, 831
PspGI CCWGG 4 cut(s) 146, 1472, 1480, 1666
PspN4I GGNNCC 6 cut(s) 152, 480, 1017, 1056, 1511, 1653
PspPI GGNCC 5 cut(s) 151, 849, 1280, 1510, 1652
PspPPI RGGWCCY 2 cut(s) 151, 1280
PspXI VCTCGAGB 1 cut(s) 930
PstI CTGCAG 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 3 cut(s) 478, 1224, 1499
RsaI GTAC 4 cut(s) 45, 123, 517, 1454
RsaNI GTAC 4 cut(s) 44, 122, 516, 1453
RseI CAYNNNNRTG 1 cut(s) 1727
SacI GAGCTC 1 cut(s) 1414
SaqAI TTAA 7 cut(s) 36, 201, 273, 422, 567, 672, 1556
SatI GCNGC 7 cut(s) 18, 89, 227, 247, 1478, 1486, 1742
Sau3AI GATC 8 cut(s) 478, 603, 808, 1224, 1381, 1436, 1499, 1660
Sau96I GGNCC 5 cut(s) 151, 849, 1280, 1510, 1652
SchI GAGTC 3 cut(s) 224, 249, 1568
ScrFI CCNGG 4 cut(s) 148, 1474, 1482, 1668
SduI GDGCHC 7 cut(s) 144, 183, 924, 1018, 1267, 1346, 1414
SfaNI GCATC 1 cut(s) 1220
SfcI CTRYAG 1 cut(s) 244
Sfr274I CTCGAG 1 cut(s) 930
SinI GGWCC 4 cut(s) 151, 849, 1280, 1510
SlaI CTCGAG 1 cut(s) 930
SmiMI CAYNNNNRTG 1 cut(s) 1727
SmlI CTYRAG 2 cut(s) 930, 1576
SmoI CTYRAG 2 cut(s) 930, 1576
SpeI ACTAGT 1 cut(s) 1550
SsiI CCGC 6 cut(s) 226, 229, 339, 829, 1153, 1604
SspI AATATT 1 cut(s) 1736
SspMI CTAG 4 cut(s) 117, 125, 989, 1551
SstI GAGCTC 1 cut(s) 1414
StyD4I CCNGG 4 cut(s) 146, 1472, 1480, 1666
StyI CCWWGG 3 cut(s) 192, 489, 597
TaaI ACNGT 4 cut(s) 719, 781, 844, 1126
TaiI ACGT 4 cut(s) 72, 567, 878, 1547
TaqI TCGA 4 cut(s) 931, 1006, 1164, 1308
TatI WGTACW 2 cut(s) 43, 1452
TauI GCSGC 1 cut(s) 229
TfiI GAWTC 3 cut(s) 701, 1180, 1244
Tru1I TTAA 7 cut(s) 36, 201, 273, 422, 567, 672, 1556
Tru9I TTAA 7 cut(s) 36, 201, 273, 422, 567, 672, 1556
TscAI CASTG 7 cut(s) 34, 57, 226, 751, 1146, 1320, 1576
TseFI GTSAC 4 cut(s) 775, 1141, 1402, 1571
TseI GCWGC 6 cut(s) 17, 88, 246, 1477, 1485, 1741
Tsp45I GTSAC 4 cut(s) 775, 1141, 1402, 1571
TspDTI ATGAA 5 cut(s) 692, 1363, 1472, 1539, 1634
TspGWI ACGGA 1 cut(s) 1147
TspRI CASTG 7 cut(s) 34, 57, 226, 751, 1146, 1320, 1576
VpaK11BI GGWCC 4 cut(s) 151, 849, 1280, 1510
XapI RAATTY 4 cut(s) 508, 679, 999, 1622
XceI RCATGY 1 cut(s) 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 1256
XhoI CTCGAG 1 cut(s) 930
XmiI GTMKAC 2 cut(s) 1395, 1547
XmnI GAANNNNTTC 1 cut(s) 1328
XspI CTAG 4 cut(s) 117, 125, 989, 1551
ZraI GACGTC 1 cut(s) 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.