MD05G1294000.v1.1

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003665
Physical Location & Seq
Reverse (-)
42665302 .. 42669726
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1294000.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGAAAGACACAGACCCTTCTTTTCCTATGGAATCCTCTGCTGTGGCGGCGAGAAGCTCCAGCTCTGCCGCGCTGACCACCACGCTCTCCAACTCAATCCAGGCTCTGGGTCGCGGTTTTGACGTCACTTCCGATATCAGGCTTCTCTACTGCAAAGGGGCACCCGGGTCGAGGCTCGTGATTCTGGAGGAGGATCAGGGGAAAGATCTTGTTCTTTCTGATGGGGTTGTTGTGCCGAATGTGCCCATTGATGTTGAGTGCGCGCCGGGGAAGAGGTCCATTGATAGAATTCACGTCTGCAGCTTCCACGAGATGGCAGAATACTTCAACAAGAAATCAGATGTATCAGGAAGCATTCCTCTTGGAAGCTTCAATGCCATGTTCAATTTCACCGGTTCTTGGCAAGTTGATGCAGCAGCAACCAAATCCCTCGCCATGATTGGATACTTCATTCCCCTATACAAAGTTAAATTAGAAAAGGATAATTTAGTTTTGCATGAGGAAATCAAGCGTGCTGTTCCTTACTCATGGGATCCTGCAGCCTTAGCAAGTTTTATCGAGAGTTATGGTACCCATATCGTCATATCTGCAACTATTGGTGGAAGAGATGTATTCTATATCAGGCAGCACAAGTCATCTCCTTTGTCAGCGGGGGACATTGAGAATTATGTGAAAGACATTGGTGATCATAGGTTTGTGGACTCAAAAAACCAGTCAAATCCAGGCCCATTAAAGTACAAGGATAAGGATGTTACAGTCATATTCAGGAGAAGAGGGGGTGATGATCTTGAGCAGAGTCATTCCAAGTGGGCCGAAACTGTACAACTAGCACCAGATGTGATTAACATGACATTTACACCCATTGTTTCTTTGCTTGAAGGAGTGCCTGGAATAAAGCACTTGAGTCGTGCGGTTGAGTTATATTTAGAGTACAAGCCACCAATCGAAGATCTGCAGTATTTCTTGGATTTTCAAATTGCTCAAGTTTGGGCCCCGGAGCAAAATAATCTGCAAAGAAAAGAACCTGTGTGTTCATCGCTTCAGTTCAGCTTGATGGGTCCGAAGCTTTACATCAGTCCAGATCAGGTAACAGTAGGCCGTAAGCCTGTCACAGGGCTTAGACTAAATCTAGAAGGCAGCAAACAGAACCGACTGGCTATACATTTGCAACATCTAGTGTCCCTTCCGAAAATTCTTCAACCCCATTGGGATGTGCACATGGCGATAGGTGCACCCAAGTGGCAAGGTCCTGAAGAGCAAGACAGCCGTTGGTTTGAGCCAATCAAGTGGAAGAACTTCTCCCATGTAAGCACTGCACCGATAGAGCATGTGGAAACCTCTATCGGTGACCTCTCTGGTGTTCACATTGTCACCGGGGCTCAGCTTGGTGTATGGGATTTTGGTGCCAAAAATGTATTGCACCTCAAACTCCTCTTCTCTAAAGTACCAGGCTGCACAATTAGGCGGTCTGTTTGGGATCACACCCCAGCCACCCCGTCTTCTGCACTGAAGACGGATGGTTCTTCTTCGTCACATTTAAACAACAGAAGCTCTGATGATAAAAAGGAAAAACTAGCTAAACTAGTGGACTTGACGGAAATGTCAAAGGGGCCGCAAGATATACCAGGTCATTGGTTAGTCACCGGGGCTAAGCTTGGAGTCGACAAGGGAAAGATTGTATTGCGCGTAAAGTACTCGTTGCTGAACTACTGA

Protein Analysis

571

Amino Acids

63.16

Weight (kDa)

7.32

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 122 - 308 4.5e-30 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1600
AatII GACGTC 1 cut(s) 126
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 3 cut(s) 160, 569, 1403
AccB7I CCANNNNNTGG 2 cut(s) 106, 313
AccI GTMKAC 1 cut(s) 1662
AccII CGCG 4 cut(s) 71, 114, 263, 1686
AciI CCGC 7 cut(s) 47, 69, 114, 650, 911, 1465, 1613
AclWI GGATC 4 cut(s) 201, 527, 540, 1485
AcsI RAATTY 2 cut(s) 288, 1191
AcuI CTGAAG 3 cut(s) 1025, 1272, 1529
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 6 cut(s) 571, 737, 822, 932, 1446, 1694
AfiI CCNNNNNNNGG 6 cut(s) 106, 138, 171, 313, 399, 1112
AgeI ACCGGT 1 cut(s) 392
AgsI TTSAA 6 cut(s) 328, 373, 385, 878, 974, 1199
AhlI ACTAGT 1 cut(s) 1582
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 5 cut(s) 99, 721, 886, 1447, 1624
AleI CACNNNNGTG 1 cut(s) 1237
Alw21I GWGCWC 2 cut(s) 1218, 1234
Alw44I GTGCAC 2 cut(s) 1214, 1230
AlwI GGATC 4 cut(s) 201, 527, 540, 1485
AlwNI CAGNNNCTG 1 cut(s) 106
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 5 cut(s) 724, 810, 990, 1096, 1610
ApaI GGGCCC 1 cut(s) 994
ApaLI GTGCAC 2 cut(s) 1214, 1230
ApeKI GCWGC 7 cut(s) 300, 413, 416, 539, 625, 1137, 1452
ApoI RAATTY 2 cut(s) 288, 1191
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
AsiGI ACCGGT 1 cut(s) 392
Asp700I GAANNNNTTC 1 cut(s) 1295
Asp718I GGTACC 1 cut(s) 569
AspLEI GCGC 4 cut(s) 73, 263, 265, 1686
AspS9I GGNCC 8 cut(s) 276, 725, 810, 990, 991, 1058, 1247, 1610
AsuC2I CCSGG 6 cut(s) 165, 166, 267, 995, 1375, 1645
AsuHPI GGTGA 6 cut(s) 382, 695, 791, 1358, 1363, 1633
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 3 cut(s) 276, 1058, 1247
BaeGI GKGCMC 5 cut(s) 163, 246, 994, 1218, 1234
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 3 cut(s) 160, 569, 1403
BanII GRGCYC 2 cut(s) 994, 1381
BauI CACGAG 2 cut(s) 176, 308
BbsI GAAGAC 2 cut(s) 1491, 1517
Bbv12I GWGCWC 2 cut(s) 1218, 1234
BbvI GCAGC 7 cut(s) 312, 425, 428, 551, 637, 1149, 1439
BccI CCATC 4 cut(s) 215, 307, 1048, 1511
BceAI ACGGC 2 cut(s) 1083, 1251
BcgI CGANNNNNNTGC 4 cut(s) 150, 184, 887, 921
BciT130I CCWGG 5 cut(s) 101, 723, 888, 1449, 1626
BciVI GTATCC 1 cut(s) 437
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 6 cut(s) 165, 166, 267, 995, 1375, 1645
BcuI ACTAGT 1 cut(s) 1582
BfaI CTAG 5 cut(s) 827, 1130, 1175, 1574, 1583
BfmI CTRYAG 3 cut(s) 298, 537, 953
BfuI GTATCC 1 cut(s) 437
BglII AGATCT 2 cut(s) 205, 949
BlpI GCTNAGC 2 cut(s) 1380, 1650
BmcAI AGTACT 1 cut(s) 1694
Bme18I GGWCC 3 cut(s) 276, 1058, 1247
BmeT110I CYCGRG 1 cut(s) 164
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 8 cut(s) 276, 725, 810, 990, 991, 1058, 1247, 1610
BmiI GGNNCC 8 cut(s) 162, 534, 571, 992, 993, 1059, 1405, 1611
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 2 cut(s) 1491, 1517
BpmI CTGGAG 2 cut(s) 43, 206
Bpu10I CCTNAGC 1 cut(s) 544
Bpu1102I GCTNAGC 2 cut(s) 1380, 1650
BpuEI CTTGAG 3 cut(s) 809, 922, 966
BpuMI CCSGG 6 cut(s) 165, 166, 267, 995, 1375, 1645
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 5 cut(s) 164, 266, 993, 1374, 1644
BsaWI WCCGGW 1 cut(s) 392
Bsc4I CCNNNNNNNGG 6 cut(s) 106, 138, 171, 313, 399, 1112
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 712, 1158
BseBI CCWGG 5 cut(s) 101, 723, 888, 1449, 1626
BseDI CCNNGG 5 cut(s) 164, 266, 993, 1374, 1644
BseGI GGATG 3 cut(s) 754, 1216, 1522
BseLI CCNNNNNNNGG 6 cut(s) 106, 138, 171, 313, 399, 1112
BseMII CTCAG 1 cut(s) 1394
BseNI ACTGG 2 cut(s) 712, 1158
BsePI GCGCGC 1 cut(s) 261
BseRI GAGGAG 2 cut(s) 203, 1421
BseSI GKGCMC 5 cut(s) 163, 246, 994, 1218, 1234
BseXI GCAGC 7 cut(s) 312, 425, 428, 551, 637, 1149, 1439
BseYI CCCAGC 1 cut(s) 1486
BsgI GTGCAG 3 cut(s) 1299, 1438, 1488
Bsh1236I CGCG 4 cut(s) 71, 114, 263, 1686
BshFI GGCC 5 cut(s) 726, 812, 992, 1098, 1612
BshNI GGYRCC 3 cut(s) 160, 569, 1403
BshTI ACCGGT 1 cut(s) 392
BsiHKAI GWGCWC 2 cut(s) 1218, 1234
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 6 cut(s) 165, 266, 393, 995, 1374, 1644
BslFI GGGAC 2 cut(s) 668, 1165
BslI CCNNNNNNNGG 6 cut(s) 106, 138, 171, 313, 399, 1112
BsmFI GGGAC 2 cut(s) 668, 1165
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 5 cut(s) 726, 812, 992, 1098, 1612
BsoBI CYCGRG 1 cut(s) 164
Bsp120I GGGCCC 1 cut(s) 990
Bsp1286I GDGCHC 6 cut(s) 163, 246, 994, 1218, 1234, 1381
Bsp1407I TGTACA 1 cut(s) 820
Bsp143I GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
Bsp1720I GCTNAGC 2 cut(s) 1380, 1650
BspACI CCGC 7 cut(s) 47, 69, 114, 650, 911, 1465, 1613
BspANI GGCC 5 cut(s) 726, 812, 992, 1098, 1612
BspCNI CTCAG 1 cut(s) 1393
BspFNI CGCG 4 cut(s) 71, 114, 263, 1686
BspLI GGNNCC 8 cut(s) 162, 534, 571, 992, 993, 1059, 1405, 1611
BspMAI CTGCAG 3 cut(s) 302, 541, 957
BspPI GGATC 4 cut(s) 201, 527, 540, 1485
BspQI GCTCTTC 1 cut(s) 1248
BspT107I GGYRCC 3 cut(s) 160, 569, 1403
BsrFI RCCGGY 1 cut(s) 392
BsrGI TGTACA 1 cut(s) 820
BsrI ACTGG 2 cut(s) 712, 1158
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 5 cut(s) 164, 266, 993, 1374, 1644
BssHII GCGCGC 1 cut(s) 261
BssMI GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 2 cut(s) 176, 308
Bst2BI CACGAG 2 cut(s) 176, 308
Bst2UI CCWGG 5 cut(s) 101, 723, 888, 1449, 1626
Bst4CI ACNGT 3 cut(s) 757, 820, 1093
Bst6I CTCTTC 5 cut(s) 266, 598, 766, 1248, 1439
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 820
BstC8I GCNNGC 2 cut(s) 263, 513
BstDEI CTNAG 4 cut(s) 544, 1118, 1380, 1650
BstEII GGTNACC 1 cut(s) 1346
BstENI CCTNNNNNAGG 1 cut(s) 1110
BstF5I GGATG 3 cut(s) 754, 1216, 1522
BstFNI CGCG 4 cut(s) 71, 114, 263, 1686
BstHHI GCGC 4 cut(s) 73, 263, 265, 1686
BstKTI GATC 8 cut(s) 196, 208, 535, 688, 787, 952, 1084, 1480
BstMBI GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
BstMWI GCNNNNNNNGC 4 cut(s) 47, 241, 545, 1229
BstNI CCWGG 5 cut(s) 101, 723, 888, 1449, 1626
BstNSI RCATGY 1 cut(s) 1331
BstPI GGTNACC 1 cut(s) 1346
BstSFI CTRYAG 3 cut(s) 298, 537, 953
BstSLI GKGCMC 5 cut(s) 163, 246, 994, 1218, 1234
BstUI CGCG 4 cut(s) 71, 114, 263, 1686
BstV1I GCAGC 7 cut(s) 312, 425, 428, 551, 637, 1149, 1439
BstV2I GAAGAC 2 cut(s) 1491, 1517
BstX2I RGATCY 3 cut(s) 205, 532, 949
BstXI CCANNNNNNTGG 2 cut(s) 1287, 1632
BstYI RGATCY 3 cut(s) 205, 532, 949
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 5 cut(s) 726, 812, 992, 1098, 1612
BtgZI GCGATG 1 cut(s) 1020
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 3 cut(s) 754, 1216, 1522
BtsI GCAGTG 1 cut(s) 1311
BtsIMutI CAGTG 2 cut(s) 1311, 1505
Cac8I GCNNGC 2 cut(s) 263, 513
CaiI CAGNNNCTG 1 cut(s) 106
CfoI GCGC 4 cut(s) 73, 263, 265, 1686
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 8 cut(s) 276, 725, 810, 990, 991, 1058, 1247, 1610
Cfr9I CCCGGG 1 cut(s) 164
CsiI ACCWGGT 1 cut(s) 1624
Csp6I GTAC 6 cut(s) 570, 736, 821, 931, 1445, 1693
CspAI ACCGGT 1 cut(s) 392
CviAII CATG 8 cut(s) 379, 436, 497, 528, 847, 1219, 1304, 1328
CviQI GTAC 6 cut(s) 570, 736, 821, 931, 1445, 1693
DdeI CTNAG 4 cut(s) 544, 1118, 1380, 1650
DpnI GATC 8 cut(s) 195, 207, 534, 687, 786, 951, 1083, 1479
DpnII GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
DraI TTTAAA 1 cut(s) 1539
DrdI GACNNNNNNGTC 1 cut(s) 1600
DseDI GACNNNNNNGTC 1 cut(s) 1600
Eam1104I CTCTTC 5 cut(s) 266, 598, 766, 1248, 1439
EarI CTCTTC 5 cut(s) 266, 598, 766, 1248, 1439
Eco24I GRGCYC 2 cut(s) 994, 1381
Eco32I GATATC 1 cut(s) 136
Eco47I GGWCC 3 cut(s) 276, 1058, 1247
Eco57I CTGAAG 3 cut(s) 1025, 1272, 1529
Eco88I CYCGRG 1 cut(s) 164
Eco91I GGTNACC 1 cut(s) 1346
EcoNI CCTNNNNNAGG 1 cut(s) 1110
EcoO109I RGGNCCY 2 cut(s) 991, 1247
EcoO65I GGTNACC 1 cut(s) 1346
EcoRI GAATTC 1 cut(s) 288
EcoRII CCWGG 5 cut(s) 99, 721, 886, 1447, 1624
EcoRV GATATC 1 cut(s) 136
EcoT38I GRGCYC 2 cut(s) 994, 1381
FaeI CATG 8 cut(s) 382, 439, 500, 531, 850, 1222, 1307, 1331
FaqI GGGAC 2 cut(s) 668, 1165
FatI CATG 8 cut(s) 378, 435, 496, 527, 846, 1218, 1303, 1327
FauI CCCGC 1 cut(s) 643
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 1 cut(s) 1662
FokI GGATG 3 cut(s) 761, 1223, 1529
FriOI GRGCYC 2 cut(s) 994, 1381
FspBI CTAG 5 cut(s) 827, 1130, 1175, 1574, 1583
GlaI GCGC 4 cut(s) 72, 262, 264, 1685
GsaI CCCAGC 1 cut(s) 1490
GsuI CTGGAG 2 cut(s) 43, 206
HaeIII GGCC 5 cut(s) 726, 812, 992, 1098, 1612
HapII CCGG 6 cut(s) 165, 266, 393, 995, 1374, 1644
HhaI GCGC 4 cut(s) 73, 263, 265, 1686
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 8 cut(s) 382, 439, 500, 531, 850, 1222, 1307, 1331
Hin6I GCGC 4 cut(s) 71, 261, 263, 1684
HinP1I GCGC 4 cut(s) 71, 261, 263, 1684
HincII GTYRAC 1 cut(s) 1663
HindII GTYRAC 1 cut(s) 1663
HindIII AAGCTT 3 cut(s) 367, 1064, 1652
HinfI GANTC 6 cut(s) 32, 181, 701, 796, 904, 1659
HpaII CCGG 6 cut(s) 165, 266, 393, 995, 1374, 1644
HphI GGTGA 6 cut(s) 382, 695, 791, 1358, 1363, 1633
Hpy166II GTNNAC 6 cut(s) 700, 1216, 1232, 1363, 1588, 1663
Hpy188I TCNGA 6 cut(s) 133, 220, 340, 1062, 1188, 1555
Hpy188III TCNNGA 9 cut(s) 178, 185, 348, 559, 766, 788, 1079, 1130, 1250
Hpy8I GTNNAC 6 cut(s) 700, 1216, 1232, 1363, 1588, 1663
HpyAV CCTTC 4 cut(s) 27, 872, 1127, 1193
HpyCH4III ACNGT 3 cut(s) 757, 820, 1093
HpyCH4IV ACGT 2 cut(s) 123, 294
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 241, 545, 1229
HpyF3I CTNAG 4 cut(s) 544, 1118, 1380, 1650
HpySE526I ACGT 2 cut(s) 123, 294
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 8 cut(s) 382, 439, 500, 531, 850, 1222, 1307, 1331
HspAI GCGC 4 cut(s) 71, 261, 263, 1684
KpnI GGTACC 1 cut(s) 573
Ksp22I TGATCA 1 cut(s) 685
Kzo9I GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
LguI GCTCTTC 1 cut(s) 1248
LmnI GCTCC 2 cut(s) 62, 997
Lsp1109I GCAGC 7 cut(s) 312, 425, 428, 551, 637, 1149, 1439
LweI GCATC 1 cut(s) 400
MabI ACCWGGT 1 cut(s) 1624
MaeI CTAG 5 cut(s) 827, 1130, 1175, 1574, 1583
MaeII ACGT 2 cut(s) 123, 294
MaeIII GTNAC 8 cut(s) 124, 751, 1087, 1108, 1346, 1369, 1530, 1639
MalI GATC 8 cut(s) 195, 207, 534, 687, 786, 951, 1083, 1479
MboI GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
MflI RGATCY 3 cut(s) 205, 532, 949
MhlI GDGCHC 6 cut(s) 163, 246, 994, 1218, 1234, 1381
MluCI AATT 8 cut(s) 288, 385, 470, 484, 664, 975, 1191, 1458
MlyI GAGTC 4 cut(s) 695, 805, 913, 1668
MmeI TCCRAC 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 4 cut(s) 468, 731, 843, 1538
MslI CAYNNNNRTG 2 cut(s) 1209, 1237
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 6 cut(s) 165, 266, 393, 995, 1374, 1644
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 5 cut(s) 101, 723, 888, 1449, 1626
MvnI CGCG 4 cut(s) 71, 114, 263, 1686
MwoI GCNNNNNNNGC 4 cut(s) 47, 241, 545, 1229
NciI CCSGG 6 cut(s) 165, 166, 267, 995, 1375, 1645
NdeII GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
NlaIII CATG 8 cut(s) 382, 439, 500, 531, 850, 1222, 1307, 1331
NlaIV GGNNCC 8 cut(s) 162, 534, 571, 992, 993, 1059, 1405, 1611
NmuCI GTSAC 6 cut(s) 124, 1108, 1346, 1369, 1530, 1639
NspI RCATGY 1 cut(s) 1331
OliI CACNNNNGTG 1 cut(s) 1237
PauI GCGCGC 1 cut(s) 261
PciSI GCTCTTC 1 cut(s) 1248
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 2 cut(s) 32, 181
PflMI CCANNNNNTGG 2 cut(s) 106, 313
PinAI ACCGGT 1 cut(s) 392
PleI GAGTC 4 cut(s) 695, 804, 912, 1667
PpsI GAGTC 4 cut(s) 695, 804, 912, 1667
PpuMI RGGWCCY 1 cut(s) 1247
Psp5II RGGWCCY 1 cut(s) 1247
Psp6I CCWGG 5 cut(s) 99, 721, 886, 1447, 1624
PspEI GGTNACC 1 cut(s) 1346
PspFI CCCAGC 1 cut(s) 1486
PspGI CCWGG 5 cut(s) 99, 721, 886, 1447, 1624
PspN4I GGNNCC 8 cut(s) 162, 534, 571, 992, 993, 1059, 1405, 1611
PspOMI GGGCCC 1 cut(s) 990
PspPI GGNCC 8 cut(s) 276, 725, 810, 990, 991, 1058, 1247, 1610
PspPPI RGGWCCY 1 cut(s) 1247
PstI CTGCAG 3 cut(s) 302, 541, 957
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 3 cut(s) 205, 532, 949
PteI GCGCGC 1 cut(s) 261
RsaI GTAC 6 cut(s) 571, 737, 822, 932, 1446, 1694
RsaNI GTAC 6 cut(s) 570, 736, 821, 931, 1445, 1693
RseI CAYNNNNRTG 2 cut(s) 1209, 1237
SalI GTCGAC 1 cut(s) 1661
SapI GCTCTTC 1 cut(s) 1248
SaqAI TTAA 4 cut(s) 468, 731, 843, 1538
Sau3AI GATC 8 cut(s) 193, 205, 532, 685, 784, 949, 1081, 1477
Sau96I GGNCC 8 cut(s) 276, 725, 810, 990, 991, 1058, 1247, 1610
ScaI AGTACT 1 cut(s) 1694
SchI GAGTC 4 cut(s) 695, 805, 913, 1668
SduI GDGCHC 6 cut(s) 163, 246, 994, 1218, 1234, 1381
SexAI ACCWGGT 1 cut(s) 1624
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 3 cut(s) 298, 537, 953
SinI GGWCC 3 cut(s) 276, 1058, 1247
SmaI CCCGGG 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 1209, 1237
SmlI CTYRAG 3 cut(s) 788, 901, 981
SmoI CTYRAG 3 cut(s) 788, 901, 981
SpeI ACTAGT 1 cut(s) 1582
Sse9I AATT 8 cut(s) 288, 385, 470, 484, 664, 975, 1191, 1458
SsiI CCGC 7 cut(s) 47, 69, 114, 650, 911, 1465, 1613
SspMI CTAG 5 cut(s) 827, 1130, 1175, 1574, 1583
TaaI ACNGT 3 cut(s) 757, 820, 1093
TaiI ACGT 2 cut(s) 126, 297
TaqI TCGA 4 cut(s) 170, 558, 945, 1662
TasI AATT 8 cut(s) 288, 385, 470, 484, 664, 975, 1191, 1458
TatI WGTACW 4 cut(s) 735, 820, 930, 1692
TauI GCSGC 3 cut(s) 50, 71, 1615
TfiI GAWTC 2 cut(s) 32, 181
Tru1I TTAA 4 cut(s) 468, 731, 843, 1538
Tru9I TTAA 4 cut(s) 468, 731, 843, 1538
TscAI CASTG 2 cut(s) 1318, 1512
TseFI GTSAC 6 cut(s) 124, 1108, 1346, 1369, 1530, 1639
TseI GCWGC 7 cut(s) 300, 413, 416, 539, 625, 1137, 1452
Tsp45I GTSAC 6 cut(s) 124, 1108, 1346, 1369, 1530, 1639
TspDTI ATGAA 3 cut(s) 17, 439, 1023
TspGWI ACGGA 2 cut(s) 1529, 1610
TspMI CCCGGG 1 cut(s) 164
TspRI CASTG 2 cut(s) 1318, 1512
Van91I CCANNNNNTGG 2 cut(s) 106, 313
VneI GTGCAC 2 cut(s) 1214, 1230
VpaK11BI GGWCC 3 cut(s) 276, 1058, 1247
XagI CCTNNNNNAGG 1 cut(s) 1110
XapI RAATTY 2 cut(s) 288, 1191
XbaI TCTAGA 1 cut(s) 1129
XceI RCATGY 1 cut(s) 1331
XmaI CCCGGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 1662
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 5 cut(s) 827, 1130, 1175, 1574, 1583
ZraI GACGTC 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.