RLG00000024987

MACPF domain-containing protein CAD1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003665
Physical Location & Seq
Forward (+)
41096688 .. 41099481
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024987

Sequence Viewer

Length: 1497 bp
ATGGTTGAGTATTTTAATCAAAAGTCTAATATATCTGGAGGCTATCCTGTGGGGAGTTTCAATTTTGCATTTAGCTTCAGCGGTTCAAAGCATGTTGATATGGCAGGAACAAAGACACTTTCTATGGATGGCCTCTATGTTCCACTTGCTAATATCCATCTTTTAAAATCCCCACTAATGTTACAAGAAAATGTGAAACAGGCTATCCCAACTTGTTGGGATCCCAAATCCTTGGCAAGCTTTATTGAAAATTTTGGTACTCATGTCATAACATCTGTAACTATTGGTGGCAAAGATGTAATTTATGTTAAACAACACCAGTCTTCTCCTTTGTCAACCATGGAGATCAAGAACTACATCCAAGACATTGGAAATCAGAGGTTCTCAGACACAGAAAGCAATACAAGTTCAGTTAAAATGAATTTCAAGGATAAGGGGTCTGATTCTGGTTTATTCAACAGTCAGGGGATATACCCTCAACCCACCACTGCACCACATCTTGCTGGAAAAGAAGATGTGACTGTCATTTTCCGAAGAAGGGGAGGAGATGATCTGGAACAAAACCACACCCGGTGGGCAAGTACTGTCCGGTCCTCTCCAGATGTTATCGAGATGACATTTTTTCCAATAACAGCTCTCCTTGAAGGGGTAATTGGAAGGGAGCACCTTACTCGAGCTGTTGGCCTTTACCTTGAATATAAACCTCCAATAGAAGAACTGAGATACTTCCTAGAGTTTCAAATTTCTCGAATATGGGCTCCTGTATATGACAGAAATCTTACCCACCAAAGAAAGGAACCTGTTTGCCCATCTTTGCAATTCAGCATAATGGGGCAAAAACTCTATGTCAGCCAAGAGCAGATAACAGTTGGACGGAAGCCAGTGACAGGCTTGCGGTTATGTCTCGAAGGAAGCAAGCAGAATCGTCTTTGTATCCATCTTCAACACTTGGCATCTCTTCCAAAGATCCTTCTACCATATTGGGATTCCCATGTAGCAATTGGTGCTCCAAAGTGGCAAGGACCTGAGGAGCAGGATAGCCGATGGTTCGAGCCAGTGAAGTGGAAAAACTTCTCTCATGTGAGCACAACACCCATTGAGAACCCTGAAACCTTTATAGGCGATCTATCTGGTGTCTACATCGTCACAGGAGCTCAGCTTGGAGTGTGGGATTTCGGATCAAAAAATGTTTTGTACATGAAGCTATTATATTCCAGGCTGCCTGGCTGCACTATACGAAGATCCTTGTGGGACCATTCCCCAAATGAAAAGTCAAAGAAAGGGACGTCTACTAGTTTAAATGTCAATGCCAGTGACTCAAGTTCCGGTTCAAAAGAAAACGTTGCGGGTAACAAGTTGATGAAATTTTTGGATATGTCCGAGATGAGCAAGGGGCCACAAGATCATCCAGGTCATTGGTTGGTTACTGGTGGAAAGCTTGGTGTGGAAAAAGGAAAAATTGTCTTAAGAGTGAAATATTCACTGCTAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

499

Amino Acids

55.86

Weight (kDa)

8.98

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 18 - 230 1.7e-29 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1289
AccI GTMKAC 2 cut(s) 1137, 1289
AciI CCGC 3 cut(s) 81, 895, 1346
AclI AACGTT 1 cut(s) 1341
AclWI GGATC 5 cut(s) 215, 228, 961, 1186, 1236
AcsI RAATTY 4 cut(s) 250, 421, 741, 1364
AcuI CTGAAG 1 cut(s) 61
AcyI GRCGYC 1 cut(s) 1286
AdeI CACNNNGTG 1 cut(s) 573
AfaI GTAC 3 cut(s) 259, 583, 1196
AfiI CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
AflII CTTAAG 1 cut(s) 1465
AhlI ACTAGT 1 cut(s) 1292
AjnI CCWGG 3 cut(s) 1214, 1222, 1408
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 8 cut(s) 75, 240, 635, 677, 1154, 1159, 1204, 1438
AluI AGCT 8 cut(s) 75, 240, 635, 677, 1154, 1159, 1204, 1438
Alw21I GWGCWC 4 cut(s) 666, 1009, 1088, 1156
Alw26I GTCTC 1 cut(s) 908
AlwI GGATC 5 cut(s) 215, 228, 961, 1186, 1236
Ama87I CYCGRG 1 cut(s) 672
AoxI GGCC 3 cut(s) 130, 682, 1394
ApeKI GCWGC 2 cut(s) 1219, 1227
ApoI RAATTY 4 cut(s) 250, 421, 741, 1364
ArsI GACNNNNNNTTYG 2 cut(s) 1256, 1288
Asp700I GAANNNNTTC 1 cut(s) 1070
AspS9I GGNCC 4 cut(s) 591, 1022, 1252, 1394
AsuC2I CCSGG 1 cut(s) 571
AvaI CYCGRG 1 cut(s) 672
AvaII GGWCC 3 cut(s) 591, 1022, 1252
AxyI CCTNAGG 1 cut(s) 1026
BaeI ACNNNNGTAYC 2 cut(s) 249, 282
BamHI GGATCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 760, 1156
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 4 cut(s) 666, 1009, 1088, 1156
BbvI GCAGC 2 cut(s) 1206, 1214
BccI CCATC 5 cut(s) 122, 165, 817, 945, 1038
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 3 cut(s) 1216, 1224, 1410
BciVI GTATCC 1 cut(s) 944
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 908
BcuI ACTAGT 1 cut(s) 1292
BfaI CTAG 2 cut(s) 731, 1293
BfrI CTTAAG 1 cut(s) 1465
BfuI GTATCC 1 cut(s) 944
BisI GCNGC 2 cut(s) 1220, 1228
BlpI GCTNAGC 1 cut(s) 1155
BlsI GCNGC 2 cut(s) 1221, 1229
BmcAI AGTACT 1 cut(s) 583
Bme1390I CCNGG 4 cut(s) 571, 1216, 1224, 1410
Bme18I GGWCC 3 cut(s) 591, 1022, 1252
BmeT110I CYCGRG 1 cut(s) 672
BmgT120I GGNCC 4 cut(s) 591, 1022, 1252, 1394
BmiI GGNNCC 5 cut(s) 222, 759, 798, 1253, 1395
BmrFI CCNGG 4 cut(s) 571, 1216, 1224, 1410
BmsI GCATC 1 cut(s) 962
BpiI GAAGAC 1 cut(s) 315
BpmI CTGGAG 2 cut(s) 57, 582
Bpu1102I GCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 1 cut(s) 1303
BpuMI CCSGG 1 cut(s) 571
BsaHI GRCGYC 1 cut(s) 1286
BsaJI CCNNGG 2 cut(s) 231, 339
BsaWI WCCGGW 2 cut(s) 588, 1325
Bsc4I CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
Bse1I ACTGG 5 cut(s) 319, 881, 1055, 1311, 1432
Bse21I CCTNAGG 1 cut(s) 1026
BseBI CCWGG 3 cut(s) 1216, 1224, 1410
BseDI CCNNGG 2 cut(s) 231, 339
BseGI GGATG 3 cut(s) 133, 357, 1405
BseLI CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
BseMII CTCAG 4 cut(s) 399, 710, 1017, 1169
BseNI ACTGG 5 cut(s) 319, 881, 1055, 1311, 1432
BseRI GAGGAG 2 cut(s) 558, 1043
BseXI GCAGC 2 cut(s) 1206, 1214
BsgI GTGCAG 2 cut(s) 474, 1213
BshFI GGCC 3 cut(s) 132, 684, 1396
BsiHKAI GWGCWC 4 cut(s) 666, 1009, 1088, 1156
BsiHKCI CYCGRG 1 cut(s) 672
BsiSI CCGG 3 cut(s) 571, 589, 1326
BslFI GGGAC 2 cut(s) 1265, 1297
BslI CCNNNNNNNGG 4 cut(s) 538, 646, 793, 887
BsmAI GTCTC 1 cut(s) 908
BsmFI GGGAC 2 cut(s) 1265, 1297
BsnI GGCC 3 cut(s) 132, 684, 1396
BsoBI CYCGRG 1 cut(s) 672
Bsp1286I GDGCHC 5 cut(s) 666, 760, 1009, 1088, 1156
Bsp1407I TGTACA 1 cut(s) 1194
Bsp143I GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
Bsp1720I GCTNAGC 1 cut(s) 1155
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 81, 895, 1346
BspANI GGCC 3 cut(s) 132, 684, 1396
BspCNI CTCAG 4 cut(s) 398, 711, 1018, 1168
BspLI GGNNCC 5 cut(s) 222, 759, 798, 1253, 1395
BspPI GGATC 5 cut(s) 215, 228, 961, 1186, 1236
BspTI CTTAAG 1 cut(s) 1465
BsrGI TGTACA 1 cut(s) 1194
BsrI ACTGG 5 cut(s) 319, 881, 1055, 1311, 1432
BssECI CCNNGG 2 cut(s) 231, 339
BssMI GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
BssNI GRCGYC 1 cut(s) 1286
BssT1I CCWWGG 2 cut(s) 231, 339
Bst2UI CCWGG 3 cut(s) 1216, 1224, 1410
Bst4CI ACNGT 4 cut(s) 461, 523, 586, 868
Bst6I CTCTTC 1 cut(s) 963
BstACI GRCGYC 1 cut(s) 1286
BstAFI CTTAAG 1 cut(s) 1465
BstAPI GCANNNNNTGC 1 cut(s) 1004
BstAUI TGTACA 1 cut(s) 1194
BstC8I GCNNGC 3 cut(s) 238, 893, 917
BstDEI CTNAG 4 cut(s) 385, 719, 1026, 1155
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 133, 357, 1405
BstKTI GATC 8 cut(s) 223, 348, 553, 969, 1126, 1181, 1244, 1405
BstMAI GTCTC 1 cut(s) 908
BstMBI GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
BstMWI GCNNNNNNNGC 1 cut(s) 1004
BstNI CCWGG 3 cut(s) 1216, 1224, 1410
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 4 cut(s) 569, 1214, 1222, 1408
BstV1I GCAGC 2 cut(s) 1206, 1214
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 3 cut(s) 220, 966, 1241
BstXI CCANNNNNNTGG 5 cut(s) 216, 232, 368, 1062, 1416
BstYI RGATCY 3 cut(s) 220, 966, 1241
Bsu36I CCTNAGG 1 cut(s) 1026
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 3 cut(s) 132, 684, 1396
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 3 cut(s) 133, 357, 1405
BtsI GCAGTG 2 cut(s) 486, 1481
BtsIMutI CAGTG 5 cut(s) 486, 888, 1062, 1318, 1481
Cac8I GCNNGC 3 cut(s) 238, 893, 917
Cfr13I GGNCC 4 cut(s) 591, 1022, 1252, 1394
Csp6I GTAC 3 cut(s) 258, 582, 1195
CviAII CATG 6 cut(s) 92, 263, 340, 992, 1079, 1198
CviQI GTAC 3 cut(s) 258, 582, 1195
DdeI CTNAG 4 cut(s) 385, 719, 1026, 1155
DpnI GATC 8 cut(s) 222, 347, 552, 968, 1125, 1180, 1243, 1404
DpnII GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
DraI TTTAAA 2 cut(s) 165, 1299
DraIII CACNNNGTG 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
Ecl136II GAGCTC 1 cut(s) 1154
Eco130I CCWWGG 2 cut(s) 231, 339
Eco24I GRGCYC 2 cut(s) 760, 1156
Eco47I GGWCC 3 cut(s) 591, 1022, 1252
Eco53kI GAGCTC 1 cut(s) 1154
Eco57I CTGAAG 1 cut(s) 61
Eco81I CCTNAGG 1 cut(s) 1026
Eco88I CYCGRG 1 cut(s) 672
EcoICRI GAGCTC 1 cut(s) 1154
EcoO109I RGGNCCY 1 cut(s) 1022
EcoRII CCWGG 3 cut(s) 1214, 1222, 1408
EcoT14I CCWWGG 2 cut(s) 231, 339
EcoT38I GRGCYC 2 cut(s) 760, 1156
ErhI CCWWGG 2 cut(s) 231, 339
FaeI CATG 6 cut(s) 95, 266, 343, 995, 1082, 1201
FaqI GGGAC 2 cut(s) 1265, 1297
FatI CATG 6 cut(s) 91, 262, 339, 991, 1078, 1197
FauI CCCGC 1 cut(s) 1339
FblI GTMKAC 2 cut(s) 1137, 1289
Fnu4HI GCNGC 2 cut(s) 1220, 1228
FokI GGATG 3 cut(s) 140, 344, 1392
FriOI GRGCYC 2 cut(s) 760, 1156
Fsp4HI GCNGC 2 cut(s) 1220, 1228
FspBI CTAG 2 cut(s) 731, 1293
GluI GCNGC 2 cut(s) 1220, 1228
GsuI CTGGAG 2 cut(s) 57, 582
HaeIII GGCC 3 cut(s) 132, 684, 1396
HapII CCGG 3 cut(s) 571, 589, 1326
Hin1I GRCGYC 1 cut(s) 1286
Hin1II CATG 6 cut(s) 95, 266, 343, 995, 1082, 1201
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 2 cut(s) 238, 1436
HinfI GANTC 4 cut(s) 443, 922, 986, 1316
HpaII CCGG 3 cut(s) 571, 589, 1326
Hpy166II GTNNAC 3 cut(s) 336, 1138, 1290
Hpy188I TCNGA 6 cut(s) 378, 388, 442, 533, 1178, 1381
Hpy188III TCNNGA 7 cut(s) 36, 349, 554, 599, 610, 747, 905
Hpy8I GTNNAC 3 cut(s) 336, 1138, 1290
HpyAV CCTTC 5 cut(s) 531, 638, 651, 902, 980
HpyCH4III ACNGT 4 cut(s) 461, 523, 586, 868
HpyCH4IV ACGT 2 cut(s) 1286, 1341
HpyCH4V TGCA 4 cut(s) 68, 491, 817, 1230
HpyF10VI GCNNNNNNNGC 1 cut(s) 1004
HpyF3I CTNAG 4 cut(s) 385, 719, 1026, 1155
HpySE526I ACGT 2 cut(s) 1286, 1341
Hsp92I GRCGYC 1 cut(s) 1286
Hsp92II CATG 6 cut(s) 95, 266, 343, 995, 1082, 1201
Kzo9I GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
LmnI GCTCC 5 cut(s) 661, 763, 1012, 1030, 1151
Lsp1109I GCAGC 2 cut(s) 1206, 1214
LweI GCATC 1 cut(s) 962
MaeI CTAG 2 cut(s) 731, 1293
MaeII ACGT 2 cut(s) 1286, 1341
MaeIII GTNAC 8 cut(s) 180, 277, 517, 883, 1144, 1313, 1349, 1423
MalI GATC 8 cut(s) 222, 347, 552, 968, 1125, 1180, 1243, 1404
MboI GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
MboII GAAGA 7 cut(s) 315, 524, 546, 725, 932, 950, 1251
MfeI CAATTG 1 cut(s) 999
MflI RGATCY 3 cut(s) 220, 966, 1241
MhlI GDGCHC 5 cut(s) 666, 760, 1009, 1088, 1156
MlyI GAGTC 1 cut(s) 1310
MmeI TCCRAC 1 cut(s) 850
MnlI CCTC 8 cut(s) 32, 143, 372, 486, 536, 604, 714, 1021
MroXI GAANNNNTTC 1 cut(s) 1070
MseI TTAA 6 cut(s) 15, 164, 309, 414, 1298, 1466
MspA1I CMGCKG 1 cut(s) 81
MspCI CTTAAG 1 cut(s) 1465
MspI CCGG 3 cut(s) 571, 589, 1326
MspR9I CCNGG 4 cut(s) 571, 1216, 1224, 1410
MunI CAATTG 1 cut(s) 999
MvaI CCWGG 3 cut(s) 1216, 1224, 1410
MwoI GCNNNNNNNGC 1 cut(s) 1004
NciI CCSGG 1 cut(s) 571
NcoI CCATGG 1 cut(s) 339
NdeII GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
NlaIII CATG 6 cut(s) 95, 266, 343, 995, 1082, 1201
NlaIV GGNNCC 5 cut(s) 222, 759, 798, 1253, 1395
NmuCI GTSAC 4 cut(s) 517, 883, 1144, 1313
NspI RCATGY 1 cut(s) 95
PaeR7I CTCGAG 1 cut(s) 672
PdmI GAANNNNTTC 1 cut(s) 1070
PfeI GAWTC 3 cut(s) 443, 922, 986
PkrI GCNGC 2 cut(s) 1221, 1229
PleI GAGTC 1 cut(s) 1310
PpsI GAGTC 1 cut(s) 1310
PpuMI RGGWCCY 1 cut(s) 1022
Psp124BI GAGCTC 1 cut(s) 1156
Psp1406I AACGTT 1 cut(s) 1341
Psp5II RGGWCCY 1 cut(s) 1022
Psp6I CCWGG 3 cut(s) 1214, 1222, 1408
PspGI CCWGG 3 cut(s) 1214, 1222, 1408
PspN4I GGNNCC 5 cut(s) 222, 759, 798, 1253, 1395
PspPI GGNCC 4 cut(s) 591, 1022, 1252, 1394
PspPPI RGGWCCY 1 cut(s) 1022
PspXI VCTCGAGB 1 cut(s) 672
PsuI RGATCY 3 cut(s) 220, 966, 1241
RsaI GTAC 3 cut(s) 259, 583, 1196
RsaNI GTAC 3 cut(s) 258, 582, 1195
SacI GAGCTC 1 cut(s) 1156
SaqAI TTAA 6 cut(s) 15, 164, 309, 414, 1298, 1466
SatI GCNGC 2 cut(s) 1220, 1228
Sau3AI GATC 8 cut(s) 220, 345, 550, 966, 1123, 1178, 1241, 1402
Sau96I GGNCC 4 cut(s) 591, 1022, 1252, 1394
ScaI AGTACT 1 cut(s) 583
SchI GAGTC 1 cut(s) 1310
ScrFI CCNGG 4 cut(s) 571, 1216, 1224, 1410
SduI GDGCHC 5 cut(s) 666, 760, 1009, 1088, 1156
SfaNI GCATC 1 cut(s) 962
Sfr274I CTCGAG 1 cut(s) 672
SinI GGWCC 3 cut(s) 591, 1022, 1252
SlaI CTCGAG 1 cut(s) 672
SmlI CTYRAG 3 cut(s) 672, 1318, 1465
SmoI CTYRAG 3 cut(s) 672, 1318, 1465
SpeI ACTAGT 1 cut(s) 1292
SsiI CCGC 3 cut(s) 81, 895, 1346
SspI AATATT 1 cut(s) 1478
SspMI CTAG 2 cut(s) 731, 1293
SstI GAGCTC 1 cut(s) 1156
StyD4I CCNGG 4 cut(s) 569, 1214, 1222, 1408
StyI CCWWGG 2 cut(s) 231, 339
TaaI ACNGT 4 cut(s) 461, 523, 586, 868
TaiI ACGT 2 cut(s) 1289, 1344
TaqI TCGA 5 cut(s) 609, 673, 748, 906, 1050
TatI WGTACW 2 cut(s) 581, 1194
TfiI GAWTC 3 cut(s) 443, 922, 986
Tru1I TTAA 6 cut(s) 15, 164, 309, 414, 1298, 1466
Tru9I TTAA 6 cut(s) 15, 164, 309, 414, 1298, 1466
TscAI CASTG 5 cut(s) 493, 888, 1062, 1318, 1488
TseFI GTSAC 4 cut(s) 517, 883, 1144, 1313
TseI GCWGC 2 cut(s) 1219, 1227
Tsp45I GTSAC 4 cut(s) 517, 883, 1144, 1313
TspDTI ATGAA 4 cut(s) 434, 1214, 1281, 1376
TspGWI ACGGA 1 cut(s) 889
TspRI CASTG 5 cut(s) 493, 888, 1062, 1318, 1488
Vha464I CTTAAG 1 cut(s) 1465
VpaK11BI GGWCC 3 cut(s) 591, 1022, 1252
XapI RAATTY 4 cut(s) 250, 421, 741, 1364
XceI RCATGY 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XhoI CTCGAG 1 cut(s) 672
XmiI GTMKAC 2 cut(s) 1137, 1289
XmnI GAANNNNTTC 1 cut(s) 1070
XspI CTAG 2 cut(s) 731, 1293
ZraI GACGTC 1 cut(s) 1287
ZrmI AGTACT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.