Rmu_sc0031054.1_g000001

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0031054.1
OG0021647
Physical Location & Seq
Reverse (-)
2571 .. 3081
511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0031054.1_g000001.1.cds

Sequence Viewer

Length: 435 bp
atgtggcctcttaactggcggtggctttcgccatgcatgatggggggaggctgtaccgatactgttccgaacaagggggaggacgcagatctttttctggtgattagccttgcttcctcggacccaacagtgactgtgtcagtgtcgcaacaattcattgcggcggggatctcactcctacaagcagaaggtcctcactacgtgaccatggtttacaagagggcaatgatggtgattggtgattatgacggcctcagcgatgtgatggatgttccgttcaatcgcttgcctatttgggtcgagatccatggattgctgctcgcttacactgcgaccatatgtgcgcaaaagctgggatctttttggccgatcagttcctccacgtggacaagcatcacttggagtgggaagaagttgtttgggttctggtgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

144

Amino Acids

15.95

Weight (kDa)

5.09

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021647)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co7990260.1_g000001 Rmu_sc0001701.1_g000006 Rmu_sc0031054.1_g000001
rosa_roxburghii Rroxscaffold_3G00249640
rosa_samantha Rh4BG098200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 345
AciI CCGC 3 cut(s) 19, 161, 164
AclWI GGATC 3 cut(s) 176, 298, 364
AcoI YGGCCR 1 cut(s) 365
AcvI CACGTG 1 cut(s) 384
AdeI CACNNNGTG 1 cut(s) 202
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 74, 384
AgsI TTSAA 1 cut(s) 280
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 1 cut(s) 352
AluI AGCT 1 cut(s) 352
AlwI GGATC 3 cut(s) 176, 298, 364
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 3 cut(s) 5, 250, 365
ApeKI GCWGC 1 cut(s) 316
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 2 cut(s) 121, 191
AsuHPI GGTGA 3 cut(s) 112, 244, 251
AvaII GGWCC 2 cut(s) 121, 191
BbrPI CACGTG 1 cut(s) 384
BbvCI CCTCAGC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 303
BccI CCATC 3 cut(s) 34, 223, 259
BceAI ACGGC 1 cut(s) 265
BglII AGATCT 1 cut(s) 88
BisI GCNGC 2 cut(s) 162, 317
BlsI GCNGC 2 cut(s) 163, 318
Bme18I GGWCC 2 cut(s) 121, 191
BmgT120I GGNCC 2 cut(s) 121, 191
BmiI GGNNCC 1 cut(s) 123
BmsI GCATC 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 254
BsaAI YACGTR 2 cut(s) 202, 384
BsaJI CCNNGG 3 cut(s) 117, 207, 307
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 384
Bse1I ACTGG 1 cut(s) 20
Bse3DI GCAATG 2 cut(s) 156, 231
BseDI CCNNGG 3 cut(s) 117, 207, 307
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 2 cut(s) 74, 384
BseMI GCAATG 2 cut(s) 156, 231
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 303
BseYI CCCAGC 1 cut(s) 352
BshFI GGCC 3 cut(s) 7, 252, 367
BslI CCNNNNNNNGG 2 cut(s) 74, 384
BsnI GGCC 3 cut(s) 7, 252, 367
Bsp143I GATC 5 cut(s) 88, 168, 303, 356, 369
Bsp19I CCATGG 2 cut(s) 207, 307
BspACI CCGC 3 cut(s) 19, 161, 164
BspANI GGCC 3 cut(s) 7, 252, 367
BspCNI CTCAG 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 3 cut(s) 176, 298, 364
BsrDI GCAATG 2 cut(s) 156, 231
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 3 cut(s) 117, 207, 307
BssMI GATC 5 cut(s) 88, 168, 303, 356, 369
BssT1I CCWWGG 2 cut(s) 207, 307
Bst4CI ACNGT 3 cut(s) 64, 130, 136
BstBAI YACGTR 2 cut(s) 202, 384
BstC8I GCNNGC 2 cut(s) 287, 321
BstDEI CTNAG 1 cut(s) 254
BstDSI CCRYGG 2 cut(s) 207, 307
BstF5I GGATG 1 cut(s) 274
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 5 cut(s) 91, 171, 306, 359, 372
BstMBI GATC 5 cut(s) 88, 168, 303, 356, 369
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 303
BstX2I RGATCY 4 cut(s) 88, 168, 303, 356
BstYI RGATCY 4 cut(s) 88, 168, 303, 356
BsuRI GGCC 3 cut(s) 7, 252, 367
BtgI CCRYGG 2 cut(s) 207, 307
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 3 cut(s) 135, 147, 327
Cac8I GCNNGC 2 cut(s) 287, 321
CaiI CAGNNNCTG 1 cut(s) 134
CfoI GCGC 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 121, 191
CseI GACGC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 54
CviAII CATG 4 cut(s) 33, 37, 208, 308
CviJI RGCY 7 cut(s) 7, 25, 51, 108, 252, 352, 367
CviKI_1 RGCY 7 cut(s) 7, 25, 51, 108, 252, 352, 367
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 1 cut(s) 254
DpnI GATC 5 cut(s) 90, 170, 305, 358, 371
DpnII GATC 5 cut(s) 88, 168, 303, 356, 369
DraIII CACNNNGTG 1 cut(s) 202
EaeI YGGCCR 1 cut(s) 365
Eco130I CCWWGG 2 cut(s) 207, 307
Eco47I GGWCC 2 cut(s) 121, 191
Eco72I CACGTG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 191
EcoT14I CCWWGG 2 cut(s) 207, 307
EcoT22I ATGCAT 1 cut(s) 38
ErhI CCWWGG 2 cut(s) 207, 307
FaeI CATG 4 cut(s) 36, 40, 211, 311
FaiI YATR 7 cut(s) 34, 38, 209, 246, 309, 338, 340
FalI AAGNNNNNCTT 2 cut(s) 382, 414
FatI CATG 4 cut(s) 32, 36, 207, 307
FauI CCCGC 1 cut(s) 157
FauNDI CATATG 1 cut(s) 338
Fnu4HI GCNGC 2 cut(s) 162, 317
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 2 cut(s) 162, 317
FspI TGCGCA 1 cut(s) 345
GlaI GCGC 1 cut(s) 345
GluI GCNGC 2 cut(s) 162, 317
GsaI CCCAGC 1 cut(s) 356
HaeIII GGCC 3 cut(s) 7, 252, 367
HgaI GACGC 1 cut(s) 92
HhaI GCGC 1 cut(s) 346
Hin1II CATG 4 cut(s) 36, 40, 211, 311
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HphI GGTGA 3 cut(s) 112, 244, 251
Hpy166II GTNNAC 2 cut(s) 214, 387
Hpy188I TCNGA 2 cut(s) 69, 121
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 2 cut(s) 214, 387
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 3 cut(s) 64, 130, 136
HpyCH4IV ACGT 2 cut(s) 201, 383
HpyCH4V TGCA 1 cut(s) 36
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 2 cut(s) 201, 383
Hsp92II CATG 4 cut(s) 36, 40, 211, 311
HspAI GCGC 1 cut(s) 344
Kzo9I GATC 5 cut(s) 88, 168, 303, 356, 369
LpnPI CCDG 3 cut(s) 83, 338, 412
Lsp1109I GCAGC 1 cut(s) 303
LweI GCATC 1 cut(s) 402
MaeII ACGT 2 cut(s) 201, 383
MaeIII GTNAC 2 cut(s) 130, 202
MalI GATC 5 cut(s) 90, 170, 305, 358, 371
MboI GATC 5 cut(s) 88, 168, 303, 356, 369
MboII GAAGA 1 cut(s) 421
MflI RGATCY 4 cut(s) 88, 168, 303, 356
MluCI AATT 1 cut(s) 152
MnlI CCTC 8 cut(s) 18, 41, 73, 127, 204, 213, 263, 388
Mph1103I ATGCAT 1 cut(s) 38
MseI TTAA 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 329
NcoI CCATGG 2 cut(s) 207, 307
NdeI CATATG 1 cut(s) 338
NdeII GATC 5 cut(s) 88, 168, 303, 356, 369
NlaIII CATG 4 cut(s) 36, 40, 211, 311
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 130, 202
NsbI TGCGCA 1 cut(s) 345
NsiI ATGCAT 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PflFI GACNNNGTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 163, 318
PmaCI CACGTG 1 cut(s) 384
PmlI CACGTG 1 cut(s) 384
Ppu21I YACGTR 2 cut(s) 202, 384
PpuMI RGGWCCY 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 191
PspCI CACGTG 1 cut(s) 384
PspFI CCCAGC 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 2 cut(s) 121, 191
PspPPI RGGWCCY 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 4 cut(s) 88, 168, 303, 356
PsyI GACNNNGTC 1 cut(s) 136
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 2 cut(s) 162, 317
Sau3AI GATC 5 cut(s) 88, 168, 303, 356, 369
Sau96I GGNCC 2 cut(s) 121, 191
SetI ASST 4 cut(s) 193, 204, 354, 386
SfaNI GCATC 1 cut(s) 402
SinI GGWCC 2 cut(s) 121, 191
Sse9I AATT 1 cut(s) 152
SsiI CCGC 3 cut(s) 19, 161, 164
StyI CCWWGG 2 cut(s) 207, 307
TaaI ACNGT 3 cut(s) 64, 130, 136
TaiI ACGT 2 cut(s) 204, 386
TaqI TCGA 1 cut(s) 300
TasI AATT 1 cut(s) 152
TauI GCSGC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 3 cut(s) 135, 147, 334
TseFI GTSAC 2 cut(s) 130, 202
TseI GCWGC 1 cut(s) 316
Tsp45I GTSAC 2 cut(s) 130, 202
TspDTI ATGAA 1 cut(s) 145
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 3 cut(s) 135, 147, 334
Tth111I GACNNNGTC 1 cut(s) 136
VpaK11BI GGWCC 2 cut(s) 121, 191
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.