Rmu_sc0041925.1_g000001

glutamine synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0041925.1
OG0031343
Physical Location & Seq
Reverse (-)
1003 .. 1674
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0041925.1_g000001.1.cds

Sequence Viewer

Length: 492 bp
atgcctggtcagtgggaattccaagttggaccaacagttggcatcgctgctggagatcaattatgggcagcgcgttacattttggagaggatcactgagatagctggagtggtggtttcatttgatccgaaaccaattcctggtgactggaacggtgctggtgcacacactaatttcagcaccaagaccatgagaaaggaaggaggctatgaggtgatcaagaaggcaattgggaagcttggattgaggcacaaggaacacattgccgcttatggcgaaggcaatgagcgccgcctcaccggacgacatgaaacagcagacattgacacattcgtatggggtgtggcgaaccgtggagcttcgataagagttgggagggacaccgagaaagaaggaaagggctattttgaggaccggaggccggcttctaacatggatccttatgttgtcacctccatgattgctgaaacaacactgctctggaaaccatag

Protein Analysis

163

Amino Acids

18.04

Weight (kDa)

7.97

Isoelectric Point (pI)

19.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0031343)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005567.1_g000001 Rmu_sc0041925.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 38, 140
AccII CGCG 1 cut(s) 73
AciI CCGC 2 cut(s) 267, 292
AclWI GGATC 4 cut(s) 98, 119, 431, 444
AcsI RAATTY 1 cut(s) 17
AfiI CCNNNNNNNGG 3 cut(s) 38, 140, 421
AjnI CCWGG 2 cut(s) 4, 139
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AluBI AGCT 3 cut(s) 104, 238, 359
AluI AGCT 3 cut(s) 104, 238, 359
Alw21I GWGCWC 1 cut(s) 166
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 4 cut(s) 98, 119, 431, 444
AoxI GGCC 1 cut(s) 419
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 47, 68
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 2 cut(s) 73, 291
AspS9I GGNCC 2 cut(s) 29, 412
AsuHPI GGTGA 4 cut(s) 155, 226, 289, 442
AvaII GGWCC 2 cut(s) 29, 412
BaeGI GKGCMC 1 cut(s) 166
BamHI GGATCC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 34, 80
BciT130I CCWGG 2 cut(s) 6, 141
BclI TGATCA 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 292
BisI GCNGC 4 cut(s) 48, 69, 267, 292
BlsI GCNGC 4 cut(s) 49, 70, 268, 293
Bme1390I CCNGG 2 cut(s) 6, 141
Bme18I GGWCC 2 cut(s) 29, 412
BmgT120I GGNCC 2 cut(s) 29, 412
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 2 cut(s) 6, 141
BmsI GCATC 1 cut(s) 51
BpmI CTGGAG 2 cut(s) 72, 126
BsaJI CCNNGG 1 cut(s) 352
BsaWI WCCGGW 2 cut(s) 299, 414
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 140, 421
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 152
Bse3DI GCAATG 2 cut(s) 261, 289
BseBI CCWGG 2 cut(s) 6, 141
BseDI CCNNGG 1 cut(s) 352
BseLI CCNNNNNNNGG 3 cut(s) 38, 140, 421
BseMI GCAATG 2 cut(s) 261, 289
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 2 cut(s) 34, 80
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 166
BsiSI CCGG 3 cut(s) 300, 415, 422
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 3 cut(s) 38, 140, 421
BsmFI GGGAC 1 cut(s) 392
BsnI GGCC 1 cut(s) 421
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 5 cut(s) 55, 90, 124, 216, 436
BspACI CCGC 2 cut(s) 267, 292
BspANI GGCC 1 cut(s) 421
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 438
BspPI GGATC 4 cut(s) 98, 119, 431, 444
BsrDI GCAATG 2 cut(s) 261, 289
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 5 cut(s) 55, 90, 124, 216, 436
Bst2UI CCWGG 2 cut(s) 6, 141
Bst4CI ACNGT 3 cut(s) 37, 155, 353
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 96
BstDSI CCRYGG 1 cut(s) 352
BstFNI CGCG 1 cut(s) 73
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 2 cut(s) 73, 291
BstKTI GATC 5 cut(s) 58, 93, 127, 219, 439
BstMBI GATC 5 cut(s) 55, 90, 124, 216, 436
BstMWI GCNNNNNNNGC 1 cut(s) 288
BstNI CCWGG 2 cut(s) 6, 141
BstSCI CCNGG 2 cut(s) 4, 139
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 2 cut(s) 34, 80
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 1 cut(s) 421
BtgI CCRYGG 1 cut(s) 352
BtgZI GCGATG 1 cut(s) 28
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 3 cut(s) 17, 93, 473
Cac8I GCNNGC 1 cut(s) 423
CfoI GCGC 2 cut(s) 73, 291
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 29, 412
CviAII CATG 4 cut(s) 190, 308, 433, 457
CviJI RGCY 7 cut(s) 104, 207, 238, 359, 402, 421, 425
CviKI_1 RGCY 7 cut(s) 104, 207, 238, 359, 402, 421, 425
DdeI CTNAG 1 cut(s) 96
DpnI GATC 5 cut(s) 57, 92, 126, 218, 438
DpnII GATC 5 cut(s) 55, 90, 124, 216, 436
Eco47I GGWCC 2 cut(s) 29, 412
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 4, 139
FaeI CATG 4 cut(s) 193, 311, 436, 460
FaqI GGGAC 1 cut(s) 392
FatI CATG 4 cut(s) 189, 307, 432, 456
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 4 cut(s) 48, 69, 267, 292
Fsp4HI GCNGC 4 cut(s) 48, 69, 267, 292
GlaI GCGC 2 cut(s) 72, 290
GluI GCNGC 4 cut(s) 48, 69, 267, 292
GsuI CTGGAG 2 cut(s) 72, 126
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 1 cut(s) 421
HapII CCGG 3 cut(s) 300, 415, 422
HhaI GCGC 2 cut(s) 73, 291
Hin1II CATG 4 cut(s) 193, 311, 436, 460
Hin6I GCGC 2 cut(s) 71, 289
HinP1I GCGC 2 cut(s) 71, 289
HindIII AAGCTT 1 cut(s) 236
HpaII CCGG 3 cut(s) 300, 415, 422
HphI GGTGA 4 cut(s) 155, 226, 289, 442
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 1 cut(s) 129
Hpy188III TCNNGA 2 cut(s) 220, 481
Hpy8I GTNNAC 1 cut(s) 164
HpyAV CCTTC 4 cut(s) 194, 217, 272, 386
HpyCH4III ACNGT 3 cut(s) 37, 155, 353
HpyCH4V TGCA 1 cut(s) 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 288
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 4 cut(s) 193, 311, 436, 460
HspAI GCGC 2 cut(s) 71, 289
KroI GCCGGC 1 cut(s) 421
KroNI GCCGGC 1 cut(s) 423
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 5 cut(s) 55, 90, 124, 216, 436
LmnI GCTCC 1 cut(s) 356
Lsp1109I GCAGC 2 cut(s) 34, 80
LweI GCATC 1 cut(s) 51
MaeIII GTNAC 3 cut(s) 74, 143, 448
MalI GATC 5 cut(s) 57, 92, 126, 218, 438
MboI GATC 5 cut(s) 55, 90, 124, 216, 436
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 5 cut(s) 17, 59, 135, 172, 228
MmeI TCCRAC 1 cut(s) 7
MnlI CCTC 9 cut(s) 81, 197, 205, 240, 305, 369, 403, 411, 463
MroNI GCCGGC 1 cut(s) 421
MslI CAYNNNNRTG 2 cut(s) 334, 455
MspI CCGG 3 cut(s) 300, 415, 422
MspR9I CCNGG 2 cut(s) 6, 141
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 2 cut(s) 6, 141
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 1 cut(s) 288
NaeI GCCGGC 1 cut(s) 423
NdeII GATC 5 cut(s) 55, 90, 124, 216, 436
NgoMIV GCCGGC 1 cut(s) 421
NlaIII CATG 4 cut(s) 193, 311, 436, 460
NlaIV GGNNCC 1 cut(s) 438
NmuCI GTSAC 2 cut(s) 143, 448
PdiI GCCGGC 1 cut(s) 423
PflMI CCANNNNNTGG 2 cut(s) 38, 140
PkrI GCNGC 4 cut(s) 49, 70, 268, 293
Psp6I CCWGG 2 cut(s) 4, 139
PspGI CCWGG 2 cut(s) 4, 139
PspN4I GGNNCC 1 cut(s) 438
PspPI GGNCC 2 cut(s) 29, 412
PsuI RGATCY 1 cut(s) 436
RseI CAYNNNNRTG 2 cut(s) 334, 455
SatI GCNGC 4 cut(s) 48, 69, 267, 292
Sau3AI GATC 5 cut(s) 55, 90, 124, 216, 436
Sau96I GGNCC 2 cut(s) 29, 412
ScrFI CCNGG 2 cut(s) 6, 141
SduI GDGCHC 1 cut(s) 166
SetI ASST 5 cut(s) 106, 216, 240, 361, 455
SfaNI GCATC 1 cut(s) 51
SinI GGWCC 2 cut(s) 29, 412
SmiMI CAYNNNNRTG 2 cut(s) 334, 455
Sse9I AATT 5 cut(s) 17, 59, 135, 172, 228
SsiI CCGC 2 cut(s) 267, 292
StyD4I CCNGG 2 cut(s) 4, 139
TaaI ACNGT 3 cut(s) 37, 155, 353
TaqI TCGA 1 cut(s) 362
TasI AATT 5 cut(s) 17, 59, 135, 172, 228
TauI GCSGC 2 cut(s) 269, 294
TscAI CASTG 3 cut(s) 17, 100, 480
TseFI GTSAC 2 cut(s) 143, 448
TseI GCWGC 2 cut(s) 47, 68
Tsp45I GTSAC 2 cut(s) 143, 448
TspDTI ATGAA 2 cut(s) 108, 324
TspRI CASTG 3 cut(s) 17, 100, 480
Van91I CCANNNNNTGG 2 cut(s) 38, 140
VneI GTGCAC 1 cut(s) 162
VpaK11BI GGWCC 2 cut(s) 29, 412
XapI RAATTY 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.