Rmu_ssc0000381.1_g000032

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000381.1
OG0025464
Physical Location & Seq
Forward (+)
147459 .. 149337
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
atggccaatcctcaacatgaagaagaagaatcaaagtcagtcgtctccattgcctctacctcattaaggaagtcaaggaaatcaaagcgtccttcctggttcacccccaagaggttacttctcatattctgtgtgatcaacttggtaaattatgtggaccgcggagcaatatcaagcaacggcgtgaatgggaaccttgcaatttgcaggaatggtaaatgcaaacctgctaccggcatacaggggcattttttactgaacaacttttcagttggatgtctatcgtcagcttttatggttggacttctggtggcttctccaatttttgcagcactagcaaagagtgtaaatccatttaagctcattggagttggattgtcaatttggacttttgctgtaactggttgtggtttttcattcaacttcttgtctatcttaatctgccgcatgctagttggtgttggtgaggcttcgtttattagtcttgcagctccgttcattgatgataatgccccagctgctcaggtattggatcttttgtttctgtttatacctattcttttgcatttgtttgacctgaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

194

Amino Acids

20.96

Weight (kDa)

9.35

Isoelectric Point (pI)

25.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 79 - 174 1.8e-08 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025464)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0021508.1_g000001 Rmu_ssc0000381.1_g000032
rosa_roxburghii Rroxscaffold_7G00199190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 235
AccII CGCG 1 cut(s) 162
AciI CCGC 3 cut(s) 160, 162, 445
AclWI GGATC 1 cut(s) 540
AcoI YGGCCR 1 cut(s) 3
AfiI CCNNNNNNNGG 3 cut(s) 66, 111, 233
AgsI TTSAA 1 cut(s) 421
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 4 cut(s) 290, 361, 491, 518
AluI AGCT 4 cut(s) 290, 361, 491, 518
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 1 cut(s) 540
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 329, 488, 518
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 2 cut(s) 94, 476
AvaII GGWCC 1 cut(s) 157
AxyI CCTNAGG 1 cut(s) 578
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 3 cut(s) 341, 500, 505
BceAI ACGGC 1 cut(s) 196
BciT130I CCWGG 1 cut(s) 97
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 2 cut(s) 335, 452
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 4 cut(s) 330, 445, 489, 519
BlsI GCNGC 4 cut(s) 331, 446, 490, 520
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 97
Bpu10I CCTNAGC 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 111, 233
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 1 cut(s) 406
Bse21I CCTNAGG 1 cut(s) 578
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 280
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 281
BseJI GATNNNNATC 1 cut(s) 280
BseLI CCNNNNNNNGG 3 cut(s) 66, 111, 233
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 2 cut(s) 536, 569
BseNI ACTGG 1 cut(s) 406
BseXI GCAGC 3 cut(s) 341, 500, 505
BseYI CCCAGC 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 3 cut(s) 66, 111, 233
BsmAI GTCTC 1 cut(s) 49
BsmBI CGTCTC 1 cut(s) 49
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 135, 532
BspACI CCGC 3 cut(s) 160, 162, 445
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 535, 570
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 135, 532
Bst2UI CCWGG 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 2 cut(s) 522, 578
BstDSI CCRYGG 1 cut(s) 160
BstENI CCTNNNNNAGG 1 cut(s) 64
BstF5I GGATG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 162
BstKTI GATC 2 cut(s) 138, 535
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 2 cut(s) 135, 532
BstMWI GCNNNNNNNGC 2 cut(s) 335, 518
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 1 cut(s) 95
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 3 cut(s) 341, 500, 505
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
Bsu36I CCTNAGG 1 cut(s) 578
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 1 cut(s) 281
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 449
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 157
Cfr42I CCGCGG 1 cut(s) 163
CseI GACGC 1 cut(s) 77
CviAII CATG 2 cut(s) 17, 448
CviJI RGCY 7 cut(s) 5, 290, 314, 361, 470, 491, 518
CviKI_1 RGCY 7 cut(s) 5, 290, 314, 361, 470, 491, 518
DdeI CTNAG 2 cut(s) 522, 578
DpnI GATC 2 cut(s) 137, 534
DpnII GATC 2 cut(s) 135, 532
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 157
Eco81I CCTNAGG 1 cut(s) 578
EcoNI CCTNNNNNAGG 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 95
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 2 cut(s) 20, 451
FaiI YATR 7 cut(s) 18, 125, 153, 239, 296, 449, 551
FatI CATG 2 cut(s) 16, 447
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 330, 445, 489, 519
FokI GGATG 1 cut(s) 288
Fsp4HI GCNGC 4 cut(s) 330, 445, 489, 519
FspBI CTAG 2 cut(s) 335, 452
GluI GCNGC 4 cut(s) 330, 445, 489, 519
GsaI CCCAGC 1 cut(s) 518
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 77
Hin1II CATG 2 cut(s) 20, 451
HinfI GANTC 1 cut(s) 29
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 94, 476
Hpy166II GTNNAC 2 cut(s) 102, 157
Hpy8I GTNNAC 2 cut(s) 102, 157
HpyAV CCTTC 1 cut(s) 102
HpyCH4V TGCA 6 cut(s) 200, 207, 222, 329, 488, 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 518
HpyF3I CTNAG 2 cut(s) 522, 578
Hsp92II CATG 2 cut(s) 20, 451
Ksp22I TGATCA 1 cut(s) 135
KspI CCGCGG 1 cut(s) 163
Kzo9I GATC 2 cut(s) 135, 532
LmnI GCTCC 2 cut(s) 164, 496
Lsp1109I GCAGC 3 cut(s) 341, 500, 505
MaeI CTAG 2 cut(s) 335, 452
MaeIII GTNAC 2 cut(s) 114, 397
MalI GATC 2 cut(s) 137, 534
MboI GATC 2 cut(s) 135, 532
MboII GAAGA 3 cut(s) 32, 35, 38
MflI RGATCY 1 cut(s) 532
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 148, 201, 321, 381
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 253, 280, 352
MnlI CCTC 6 cut(s) 21, 64, 70, 105, 460, 573
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 65, 357, 437
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 162, 518
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 335, 518
NdeII GATC 2 cut(s) 135, 532
NlaIII CATG 2 cut(s) 20, 451
NlaIV GGNNCC 1 cut(s) 194
NspI RCATGY 1 cut(s) 451
PaeI GCATGC 1 cut(s) 451
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 4 cut(s) 331, 446, 490, 520
Psp6I CCWGG 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 514
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 1 cut(s) 157
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 518
SacII CCGCGG 1 cut(s) 163
SaqAI TTAA 3 cut(s) 65, 357, 437
SatI GCNGC 4 cut(s) 330, 445, 489, 519
Sau3AI GATC 2 cut(s) 135, 532
Sau96I GGNCC 1 cut(s) 157
ScrFI CCNGG 1 cut(s) 97
Sfr303I CCGCGG 1 cut(s) 163
SgrBI CCGCGG 1 cut(s) 163
SinI GGWCC 1 cut(s) 157
SphI GCATGC 1 cut(s) 451
Sse9I AATT 4 cut(s) 148, 201, 321, 381
SsiI CCGC 3 cut(s) 160, 162, 445
SspMI CTAG 2 cut(s) 335, 452
StyD4I CCNGG 1 cut(s) 95
TasI AATT 4 cut(s) 148, 201, 321, 381
TauI GCSGC 1 cut(s) 447
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 3 cut(s) 65, 357, 437
Tru9I TTAA 3 cut(s) 65, 357, 437
TseI GCWGC 3 cut(s) 329, 488, 518
TspDTI ATGAA 3 cut(s) 33, 405, 487
TspGWI ACGGA 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 157
XagI CCTNNNNNAGG 1 cut(s) 64
XceI RCATGY 1 cut(s) 451
XspI CTAG 2 cut(s) 335, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.