Rmu_ssc0000480.1_g000021

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho- Glu-tRNA(Gln)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000480.1
OG0029508
Physical Location & Seq
Forward (+)
108772 .. 112497
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
atggcaggacaaagcattgatcttcataacaatggtaatctcctaagacaaggctggagactcaagttctttgattgtgcagatggttctcgtggattagttcatgatttgattccggtcaactatcgccggattacgtcacccgcggccactcacctgactactttgaaaattttgccggatgtccctaaagtagttgttggagatttcatcagtcgccggaaagagtttgtgtcctcctcctccgatgaagacgacgccgtcgcccacgacatggtgctcgtgatcagagagagagagtatgtcggtgacggtcatgctggggagagagagctcgtgatcagagagagagagtatgtcggtgacggtcatgctggggagagagagctcgtgatcagagagagagaccccacctccgctccttccctccaagtctcagacgccgtcctcgcccaggcctgcgacctcaacgacaggcttcgccggaatgaggagatgggaccctggccggggattttggagggctatacgccgccgtatgatgccaccaccgtcaggaagattaaggagctgggtgggattgtggtgggcaagactaatttggatgagtttgatgggaccctggccggggtccttatggcggtgaaggataacatatgtacggaggagatgccgtccacggcggggttccagggttttggagggctatacgccgccgtatga

Protein Analysis

240

Amino Acids

26.36

Weight (kDa)

5.03

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 172 - 204 2.7e-06 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0029508)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr0c29g0501181
rosa_multiflora Rmu_ssc0000480.1_g000021

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 274
AccBSI CCGCTC 1 cut(s) 419
AccII CGCG 1 cut(s) 146
AciI CCGC 7 cut(s) 144, 146, 417, 533, 641, 683, 714
AcoI YGGCCR 3 cut(s) 147, 506, 624
AcsI RAATTY 1 cut(s) 171
AcyI GRCGYC 2 cut(s) 258, 441
AfaI GTAC 1 cut(s) 661
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 4 cut(s) 453, 503, 621, 690
AluBI AGCT 3 cut(s) 334, 388, 571
AluI AGCT 3 cut(s) 334, 388, 571
Alw21I GWGCWC 3 cut(s) 282, 336, 390
Alw26I GTCTC 3 cut(s) 52, 399, 439
AoxI GGCC 4 cut(s) 147, 456, 506, 624
ApoI RAATTY 1 cut(s) 171
AspS9I GGNCC 3 cut(s) 500, 618, 631
AsuC2I CCSGG 2 cut(s) 510, 628
AsuHPI GGTGA 5 cut(s) 132, 146, 320, 374, 655
AvaII GGWCC 3 cut(s) 500, 618, 631
BanII GRGCYC 2 cut(s) 336, 390
BauI CACGAG 4 cut(s) 90, 281, 335, 389
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 3 cut(s) 282, 336, 390
BccI CCATC 3 cut(s) 77, 490, 608
BceAI ACGGC 6 cut(s) 245, 428, 520, 658, 696, 701
BciT130I CCWGG 4 cut(s) 455, 505, 623, 692
BclI TGATCA 3 cut(s) 285, 339, 393
BcnI CCSGG 2 cut(s) 510, 628
BcoDI GTCTC 3 cut(s) 52, 399, 439
BisI GCNGC 3 cut(s) 147, 533, 714
BlsI GCNGC 3 cut(s) 148, 534, 715
Bme1390I CCNGG 6 cut(s) 455, 505, 510, 623, 628, 692
Bme18I GGWCC 3 cut(s) 500, 618, 631
BmgT120I GGNCC 3 cut(s) 500, 618, 631
BmiI GGNNCC 6 cut(s) 501, 502, 619, 620, 632, 689
BmrFI CCNGG 6 cut(s) 455, 505, 510, 623, 628, 692
BmsI GCATC 2 cut(s) 532, 660
BpiI GAAGAC 1 cut(s) 258
BpmI CTGGAG 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 2 cut(s) 510, 628
BsaHI GRCGYC 2 cut(s) 258, 441
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 8 cut(s) 144, 453, 503, 509, 621, 627, 678, 691
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 6 cut(s) 398, 403, 428, 433, 659, 689
BseBI CCWGG 4 cut(s) 455, 505, 623, 692
BseDI CCNNGG 8 cut(s) 144, 453, 503, 509, 621, 627, 678, 691
BseGI GGATG 2 cut(s) 187, 610
BseMII CTCAG 1 cut(s) 450
BseRI GAGGAG 4 cut(s) 229, 232, 506, 680
BseYI CCCAGC 3 cut(s) 320, 374, 571
BsgI GTGCAG 1 cut(s) 99
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 4 cut(s) 149, 458, 508, 626
BsiHKAI GWGCWC 3 cut(s) 282, 336, 390
BsiSI CCGG 7 cut(s) 116, 130, 179, 220, 484, 509, 627
BslFI GGGAC 3 cut(s) 170, 513, 631
BsmAI GTCTC 3 cut(s) 52, 399, 439
BsmFI GGGAC 3 cut(s) 170, 513, 631
BsnI GGCC 4 cut(s) 149, 458, 508, 626
Bso31I GGTCTC 1 cut(s) 399
Bsp1286I GDGCHC 3 cut(s) 282, 336, 390
Bsp143I GATC 4 cut(s) 19, 285, 339, 393
BspACI CCGC 7 cut(s) 144, 146, 417, 533, 641, 683, 714
BspANI GGCC 4 cut(s) 149, 458, 508, 626
BspCNI CTCAG 1 cut(s) 449
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 6 cut(s) 501, 502, 619, 620, 632, 689
BspTNI GGTCTC 1 cut(s) 399
BsrBI CCGCTC 1 cut(s) 419
BssECI CCNNGG 8 cut(s) 144, 453, 503, 509, 621, 627, 678, 691
BssMI GATC 4 cut(s) 19, 285, 339, 393
BssNI GRCGYC 2 cut(s) 258, 441
BssSI CACGAG 4 cut(s) 90, 281, 335, 389
Bst2BI CACGAG 4 cut(s) 90, 281, 335, 389
Bst2UI CCWGG 4 cut(s) 455, 505, 623, 692
Bst4CI ACNGT 3 cut(s) 314, 368, 553
BstACI GRCGYC 2 cut(s) 258, 441
BstC8I GCNNGC 1 cut(s) 460
BstDEI CTNAG 2 cut(s) 44, 436
BstDSI CCRYGG 2 cut(s) 144, 678
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 2 cut(s) 187, 610
BstFNI CGCG 1 cut(s) 146
BstKTI GATC 4 cut(s) 22, 288, 342, 396
BstMAI GTCTC 3 cut(s) 52, 399, 439
BstMBI GATC 4 cut(s) 19, 285, 339, 393
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 4 cut(s) 455, 505, 623, 692
BstSCI CCNGG 6 cut(s) 453, 503, 508, 621, 626, 690
BstUI CGCG 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 258
BstXI CCANNNNNNTGG 1 cut(s) 698
BsuRI GGCC 4 cut(s) 149, 458, 508, 626
BtgI CCRYGG 2 cut(s) 144, 678
BtsCI GGATG 2 cut(s) 187, 610
Cac8I GCNNGC 1 cut(s) 460
CciI TCATGA 1 cut(s) 103
Cfr13I GGNCC 3 cut(s) 500, 618, 631
Cfr42I CCGCGG 1 cut(s) 147
CseI GACGC 2 cut(s) 266, 449
Csp6I GTAC 1 cut(s) 660
CviAII CATG 4 cut(s) 104, 274, 317, 371
CviQI GTAC 1 cut(s) 660
DdeI CTNAG 2 cut(s) 44, 436
DpnI GATC 4 cut(s) 21, 287, 341, 395
DpnII GATC 4 cut(s) 19, 285, 339, 393
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 3 cut(s) 147, 506, 624
Ecl136II GAGCTC 2 cut(s) 334, 388
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 2 cut(s) 336, 390
Eco31I GGTCTC 1 cut(s) 399
Eco47I GGWCC 3 cut(s) 500, 618, 631
Eco53kI GAGCTC 2 cut(s) 334, 388
EcoICRI GAGCTC 2 cut(s) 334, 388
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoO109I RGGNCCY 3 cut(s) 500, 618, 631
EcoRII CCWGG 4 cut(s) 453, 503, 621, 690
EcoT38I GRGCYC 2 cut(s) 336, 390
FaeI CATG 4 cut(s) 107, 277, 320, 374
FaqI GGGAC 3 cut(s) 170, 513, 631
FatI CATG 4 cut(s) 103, 273, 316, 370
FauI CCCGC 2 cut(s) 151, 676
FauNDI CATATG 1 cut(s) 656
FbaI TGATCA 3 cut(s) 285, 339, 393
Fnu4HI GCNGC 3 cut(s) 147, 533, 714
FokI GGATG 2 cut(s) 194, 617
FriOI GRGCYC 2 cut(s) 336, 390
Fsp4HI GCNGC 3 cut(s) 147, 533, 714
GluI GCNGC 3 cut(s) 147, 533, 714
GsaI CCCAGC 3 cut(s) 324, 378, 575
GsuI CTGGAG 1 cut(s) 76
HaeIII GGCC 4 cut(s) 149, 458, 508, 626
HapII CCGG 7 cut(s) 116, 130, 179, 220, 484, 509, 627
HgaI GACGC 2 cut(s) 266, 449
Hin1I GRCGYC 2 cut(s) 258, 441
Hin1II CATG 4 cut(s) 107, 277, 320, 374
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 2 cut(s) 60, 112
HpaII CCGG 7 cut(s) 116, 130, 179, 220, 484, 509, 627
HphI GGTGA 5 cut(s) 132, 146, 320, 374, 655
Hpy166II GTNNAC 2 cut(s) 121, 678
Hpy188I TCNGA 5 cut(s) 247, 290, 344, 398, 439
Hpy188III TCNNGA 5 cut(s) 104, 283, 337, 391, 556
Hpy8I GTNNAC 2 cut(s) 121, 678
Hpy99I CGWCG 2 cut(s) 260, 266
HpyAV CCTTC 2 cut(s) 432, 640
HpyCH4III ACNGT 3 cut(s) 314, 368, 553
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 1 cut(s) 80
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 2 cut(s) 44, 436
HpySE526I ACGT 1 cut(s) 137
Hsp92I GRCGYC 2 cut(s) 258, 441
Hsp92II CATG 4 cut(s) 107, 277, 320, 374
KflI GGGWCCC 2 cut(s) 500, 618
Ksp22I TGATCA 3 cut(s) 285, 339, 393
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 4 cut(s) 19, 285, 339, 393
LmnI GCTCC 2 cut(s) 424, 568
LweI GCATC 2 cut(s) 532, 660
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 3 cut(s) 138, 308, 362
MalI GATC 4 cut(s) 21, 287, 341, 395
MbiI CCGCTC 1 cut(s) 419
MboI GATC 4 cut(s) 19, 285, 339, 393
MboII GAAGA 3 cut(s) 14, 263, 571
MhlI GDGCHC 3 cut(s) 282, 336, 390
MluCI AATT 2 cut(s) 171, 598
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 181
MseI TTAA 1 cut(s) 564
MslI CAYNNNNRTG 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 7 cut(s) 116, 130, 179, 220, 484, 509, 627
MspR9I CCNGG 6 cut(s) 455, 505, 510, 623, 628, 692
MvaI CCWGG 4 cut(s) 455, 505, 623, 692
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 449
NciI CCSGG 2 cut(s) 510, 628
NdeI CATATG 1 cut(s) 656
NdeII GATC 4 cut(s) 19, 285, 339, 393
NlaIII CATG 4 cut(s) 107, 277, 320, 374
NlaIV GGNNCC 6 cut(s) 501, 502, 619, 620, 632, 689
NmuCI GTSAC 3 cut(s) 138, 308, 362
PagI TCATGA 1 cut(s) 103
PceI AGGCCT 1 cut(s) 458
PfeI GAWTC 1 cut(s) 112
PflFI GACNNNGTC 2 cut(s) 260, 443
PflMI CCANNNNNTGG 1 cut(s) 274
PkrI GCNGC 3 cut(s) 148, 534, 715
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 3 cut(s) 500, 618, 631
Psp124BI GAGCTC 2 cut(s) 336, 390
Psp5II RGGWCCY 3 cut(s) 500, 618, 631
Psp6I CCWGG 4 cut(s) 453, 503, 621, 690
PspFI CCCAGC 3 cut(s) 320, 374, 571
PspGI CCWGG 4 cut(s) 453, 503, 621, 690
PspN4I GGNNCC 6 cut(s) 501, 502, 619, 620, 632, 689
PspPI GGNCC 3 cut(s) 500, 618, 631
PspPPI RGGWCCY 3 cut(s) 500, 618, 631
PsyI GACNNNGTC 2 cut(s) 260, 443
RsaI GTAC 1 cut(s) 661
RsaNI GTAC 1 cut(s) 660
RseI CAYNNNNRTG 1 cut(s) 30
SacI GAGCTC 2 cut(s) 336, 390
SacII CCGCGG 1 cut(s) 147
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 3 cut(s) 147, 533, 714
Sau3AI GATC 4 cut(s) 19, 285, 339, 393
Sau96I GGNCC 3 cut(s) 500, 618, 631
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 6 cut(s) 455, 505, 510, 623, 628, 692
SduI GDGCHC 3 cut(s) 282, 336, 390
SetI ASST 7 cut(s) 140, 159, 336, 390, 416, 468, 573
SfaNI GCATC 2 cut(s) 532, 660
Sfr303I CCGCGG 1 cut(s) 147
SgrBI CCGCGG 1 cut(s) 147
SinI GGWCC 3 cut(s) 500, 618, 631
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 2 cut(s) 171, 598
SseBI AGGCCT 1 cut(s) 458
SsiI CCGC 7 cut(s) 144, 146, 417, 533, 641, 683, 714
SstI GAGCTC 2 cut(s) 336, 390
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 6 cut(s) 453, 503, 508, 621, 626, 690
TaaI ACNGT 3 cut(s) 314, 368, 553
TaiI ACGT 1 cut(s) 140
TasI AATT 2 cut(s) 171, 598
TauI GCSGC 3 cut(s) 149, 535, 716
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TseFI GTSAC 3 cut(s) 138, 308, 362
Tsp45I GTSAC 3 cut(s) 138, 308, 362
TspDTI ATGAA 4 cut(s) 14, 92, 199, 264
TspGWI ACGGA 1 cut(s) 677
Tth111I GACNNNGTC 2 cut(s) 260, 443
Van91I CCANNNNNTGG 1 cut(s) 274
VpaK11BI GGWCC 3 cut(s) 500, 618, 631
XagI CCTNNNNNAGG 1 cut(s) 452
XapI RAATTY 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.